UOW Laboratory Safety 2000
UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
Sheet......1.. of.......1...
******APPENDIX 10 - MICROBIAL HAZARDS (ACDP GROUPS 1 & 2 ONLY)******
Title of Experiment
Room/Laboratory
Site
1.
IDENTIFICATION PRACTICAL
Cavendish
6th floor lab
Brief description of work
Identification of 6 strains of bacteria using Cowan & Steel first-stage identification, API 20E and Enterotube
2.
LIST OF CULTURES USED (continue on a separate sheet if necessary)
Manufacturers Safety Data
Computer Data Base
Product Label
Other (Specify)
NAME OF CULTURE
CULTURE
TYPE
MAX.
QUANTITY
EXPOSED
TO
e.g. 20 ml
broth for
UB, 15 ml
agar for
plate.
DATA
AVAILABLE
KNOWN HAZARDS
(See Note;
Use numbers)
Microorganism (ACDP Category
1 or 2 only).
Give full name, strain code(s) and
UOW number (if known).
e.g. broth
culture,
Petri dish,
fermenter
Escherichia coli
Broth, plate
20ml
Mycobacterium phlei
Broth, plate
“
“
Klebsiella pneumoniae
Broth, plate
“
“
Pseudomonas aeruginosa
Broth, plate
“
“
Enterococcus faecalis
Broth, plate
“
“
AC
DP
2
1
Staphylococcus epidermidis
20 (Biological hazard).
ACDP = Advisory
Committee on Dangerous
Pathogens.
20 (Biological hazard)
ACDP Category 2
pathogens
ACDP Category 1
NOTE: 1) EXPLOSIVE 2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OPERATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
PERSONNEL INVOLVED WITH CULTURES (continue on a separate sheet if necessary)
NAME/CLASS
4.
INITS
STATUS
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Pitzulu
L
Technician
O
Miles
E
Lecturer
O
Parry
M
Lecturer
O
Redway
K
Lecturer
O
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE CULTURES
(e.g. students [name class], cleaners, maintenance staff, contractors, visitors, storekeepers, etc.)
Students on this module, Medical Microbiology (3MED666), who do this practical once in Semester 2.
Any staff or other students sharing the same laboratory or prep room at, or near, the time of this
practical.
UOW Laboratory Safety 2000
5.
EMERGENCY PROCEDURES
if any of the cultures or procedures identified above is likely to pose a special hazard in an emergency,
then identify below action to be taken
SPILLAGE/UNCONTROLLED RELEASE:
FIRE:
Follow instructions in Appendix 2 of UOW Regulations
for the Use of Biological Materials (Procedure for
dropped or spilt cultures).
N/A
If personnel are affected (fume, contamination, etc.) treatment to be adopted:
(N.B. Antidotes and special treatment may be obtained through.............N/A...............)
Wash affected area of skin with germicidal soap. Change contaminated clothes and wash with detergent and water.
If eyes affected, wash with plenty of water.
6.
CONTROL MEASURES TO BE ADOPTED
(NB. Consider following points, appropriate Biosciences Safety Codes plus any other.  = ESSENTIAL)
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
. Sufficient general ventilation


. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.)
NB. gloves essential for human body fluids but consider them for other samples also.
Goggles should also to be considered for some samples.
. Safe disposal/storage of hazardous substances/cultures
. Regular cleaning of the workplace
. Adequate washing facilities
STORAGE - SAFETY CONSIDERATIONS
HANDLING PRECAUTIONS
Use secure, fully labelled containers in fridge, freezer or
incubator.
Follow instructions in Appendix 1 of UOW Regulations
for the Use of Biological Materials (Code of Practice for
the Use of Microorganisms). Plus all students must
receive adequate practical instruction from the lecturer in
charge.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Follow instructions in Appendix 1. Technicians to follow instructions in Appendix 8 (Instructions for technical staff).
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks and their frequency, on the adequacy and maintenance of the control measures during the
course of the experiment)
(N.B. Also state if any health surveillance is necessary; are exposure time logs/maintenance/inspection test
records available for inspection? Medical monitoring not normally required for ACDP Group 1 and 2 organisms
but note para 4 of Chemical Safety Regulations (p. 26) and the footnote (p. 28). )
Lecturer in charge of class must ensure that students follow all instructions and safety procedures as detailed in
the UOW Regulations for the Use of Biological Materials, the Codes of Practice and the relevant Appendices
during the course of the experiment.
medical monitoring - NO
occupational health nurse informed - NO
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary).
All experiments must comply with the UOW Regulations for the Use of Biological Materials, the Codes of
Practice and the relevant Appendices. This form is for ACDP Group 1 and 2 microorganisms only.
Microorganisms in ACDP Groups 3 and 4 are not allowed on University property. The use of all microbial
cultures and biologically hazardous materials must be cleared with the Biological Safety Officer (Keith Redway)
before they are brought onto University property or ordered. COSHH Risk Assessment for chemicals requires a
different form (Appendix 4 of Chemical Safety Regulations.) Assessment for class activity may not be
sufficient for technician preparation - consider double risk assessment. For guidance notes on the
completion of COSHH forms see page 32.
9.
Name of Assessor:
K.Redway
Signed:
K. Redway
Status of Assessor:
Lecturer
Date:
22th February 2005
Keith Redway