Course Syllabus
BI 434 Determinative Bacteriology
Course description
To study the methods in isolation, identification and classification of bacteria including
solving the problems of bacterial classification
Third year and fourth year students
Assoc.Prof. Sariya Sindermsuk
Semeter I,
Lecture
Wednesday 10.00 – 12.00 น.
Laboratory Wednesday 13.00 – 16.00 น.
Room number
Lecture
10-503
Laboratory 10-303
Students
Instructor
Class time
Theory
Teaching schedule :
Week number
1-2
3
4
Topics
Introduction
Numerical Taxonomy
Nucleic acids in bacterial classification
Genetic methods in bacterial classification
Serology and chemotaxonomy
KINGDOM PROCARYOTAE
Division I. The Cyanobacteria
Division II The Bacteria
PHOTOTROPHIC BACTERIA
Order I. Rhodospirillales
Family I. Rhodospirillaceae
Family II. Chromatiaceae
Family III. Chlorobiaceae
2
5
6
7–8
9
10
11
THE GLIDING BACTERIA
Order I. Myxobacterales
Family I. Myxococcaceae
Family II. Archangiaceae
Family III. Cystobacteraceae
Family IV. Polyangiaceae
Order II. Cytophagales
Family I. Cytophagaceae
Family II. Beggiatoaceae
Family III. Simonsiellaceae
THE SHEATHED BACTERIA
BUDDING and/or APPENDAGED BACTERIA
THE SPIROCHETES
Order I. Spirochaetales
Family I. Spirochaetaceae
SPIRAL AND CURVED BACTERIA
Family I. sSpirillaceae
GRAM-NEGATIVE AEROBIC RODS AND COCCI
Family I. Pseudomonadaceae
Family II. Azotobacteraceae
Family III. Rhizobiaceae
Family IV. Methylomonadaceae
Family V. Halobacteriaceae
Mid-term Examination
GRAM-NEGATIVE FACULTATIVELY ANEROBIC ROOS
Family I. Enterobacteriaceae
Family II. Vibrionaceae
GRAM-NEGATIVE ANAEROBIC BACTERIA
Family I. Bacteroidaceae
GRAM-NEGATIVE COCCI AND COCCOBACILLI
Family I. Neisseriaceae
3
12
13
14
GRAM-NEGATIVE ANAEROBIC COCCI
Family I. Veillonellaceae
GRAM-NEGATIVE, CHEMOLITHOTROPHIC BACTERIA
a. Organisms oxidizing ammonia or nitrite
Family I. Nitrobacteraceae
b. Organisms metabolizing sulfur
c. Organisms depositing iron or manganese oxides
Family I. Siderocapsaceae
METHANE-PRODUCING BACTERIA
Family I. Methanobacteriaceae
GRAM-POSITIVE COCCI
a. Aerobic and/or facultatively anaerobic
Family I. Micrococcaceae
Family II. Streptococcaceae
b. Anaerobic
Family III. Peptococcaceae
ENDOSPORE-FORMING RODS AND COCCI
Family I. Bacillaceae
GRAM-POSITIVE, ASPOROGENOUS ROD-SHAPED
BACTERIA
Family I. Lactobacillaceae
ACTINOMYCETES AND RELATED ORGANISMS
Coryneform Group of Bacteria
a. Human and Animal Parasites and Pathogens
b. Plant Pathogenic Corynebacteria
c. Non-pathogenic Corynebacteria
Family I. Propionibacteriaceae
Order I. Actinomycetales
Family I. Actinomycetaceae
Family II. Mycobacteriaceae
Family III. Frankiaceae
4
15
16
Family IV. Actinoplanaceae
Family V. Dermatophilaceae
Family VI. Nocardiaceae
Family VII. Streptomycetaceae
Family VIII. Micromonosporaceae
THE RICKETTSIAS
Order I. Rickettsiales
Family I. Rickettsiaceae
Tribe I. Rickettsieae
Tribe II. Ehrlichieae
Tribe III. Wolbachieae
Family II. Bartonellaceae
Family III. Anaplasmataceae
Order II. Chlamydiales
Family I. Chlamydiaceae
THE MYCOPLASMAS
Class I. Mollicutes
Order I. Mycoplasmatales
Family I. Mycoplasmataceae
Family II. Acholeplasmataceae
Final Examination
Laboratory practices
Teaching schedule :
Week number
1
2
3
4-6
Topics
Study on CYANOBACTERIA including culturing of this organism
Cultivation of PHOTOTROPHS from water resources
Cultivation of THE GLIDING BACTERIA in soil
Study of families of the GRAM-NEGATIVE
AEROBIC RODS AND COCCI :
Fam. Pseudomonadaceae
Fam. Azotobacteraceae
5
Week number
7-9
10-11
12
13
14-15
16
Lecture :
Topics
Fam. Rhizobiaceae
Study of GRAM-NEGATIVE
FACULTATIVELY ANAEROBIC RODS :
Fam. Enterobacteriaceae
Fam. Vibrionaceae
which will be isolated from animal feces and sea food.
Study on GRAM-POSITIVE COCCI by isolation from food,
water, soil and diary products :
Fam. Micrococcaceae
Fam. Streptococcaceae
Study on ENDOSPORE FORMING ROD AND COCCI in
Fam. Bacillaceae both aerobe and anaerobe
by isolation from soil
Study on GRAM-POSITIVE ASPOROGENEOUS RODSHAPED BACTERIA in diary products including pure
culture isolation
Study on ACTINOMYCETES from soil and the submission of
culture
Final Examination
Midterm examination
Final examination
Laboratory :
submission of isolated bacteria
Paper laboratory
45%
55%
90%
10%
6
References
1.* Buchanan R. E. and Gibbons N. E., co-editors. 1975. Bergey's manual of
Determinative Bacteriology. 8th edition. Baltimore, Md: Williams & Wilkins.
2. Gibbs B. M. and Skinner F. A. 1966. Identification Methods for Microbiologist Part A
and B. Chatnam, Kent: W. and J. Mackay Co. Ltd.
3. Holt, J. G. ed. 1984. Bergey's manuals of systematic bacteriology. Vol. 1. pp. 1-23.
Baltimore, Md: Williams and Wilkins.
4. Koneman E. W. et. al. 1988. Color Atlas and Textbook of Diagnostic Microbiology. 3nd
edition. Philadelphia, Pen.. Lippincott Co.
5. Lansing M. P., Harley J. P. and Klein D. A. 1990. Microbiology. 2nd edition, pp. 404-407.
Dubuque, I A: Wm. C. Brown Publishers.
6. Norris J. R. and Ribbons D. W. 1971. Methods in Microbiology. Bortholomew press,
Dorking: Adland and Son Ltd.