Name _________________________________ Period __________ LESSON 4.2: Powering the Cell- Cellular Respiration QUICK REVIEW…. What are the two stages of photosynthesis? ____________________________________________________ What happens in the chloroplasts? ___________________________________________________________ Study the cycles, reactants and products labeled in the picture below…. Color stage/cycle names in yellow (2 items); Color reactants and products in light green (8 items) LIGHT IS ENERGY…… Light –Dependent Reactions: Energy Source: _______________ Reactants: Products: _______________ Calvin Cycle: Energy Source: Reactants: _________________ _________________ _______________ _________________ _______________ _________________ _______________ Products: _________________ _______________ _________________ _______________ _________________ WHAT PROCESS IS PICTURED ABOVE? ___________________________________ I. Breathing versus Cellular Respiration • _____________________________: physical process that allows animals and humans to come into contact with gases in the air. • _____________________________: chemical process that releases energy from organic compounds (food), gradually converting it into energy that is stored in ATP molecules 1 II. Chemical Pathways 1. What is the raw material used by cells to provide the energy your body needs to function? 2. What do cells synthesize from this raw material? 3. Cells do not BURN glucose, they slowly release energy from it and other food compounds through several pathways (processes) a. 1st pathway ______________________: releases only a small amount of energy (2 net ATP) i. If oxygen________________, it will lead to two other pathways that release a lot of energy: Krebs cycle & Electron Transport Chain ii. If oxygen ____________________: glycolysis is followed by a different pathway: Alcoholic Fermentation or Lactic Acid Fermentation 4. What does glycolysis mean literally? 5. What does aerobic mean? ___________________________________ what pathways after glycolysis follow aerobic respiration? ____________________ & _______________________________________ 6. What do we call Glycolysis followed by the Krebs Cycle and the Electron Transport Chain? 7. Write the chemical equation that shows the cellular respiration reaction: 8. Where does the glucose used in cellular respiration come from? III. Glycolysis 9. Takes place in the ___________________________________________________________________ outside of the ________________________________. 10. Define cytosol: ____________________________________________________________________________________ ____________________________________________________________________________________ 11. Define cytoplasm: ____________________________________________________________________________________ ____________________________________________________________________________________ 2 12. Glycolysis coverts _______________ with the help of ___ATP molecules and eventually releases ___ATP molecules; for a net gain of ___ ATP molecules. 13. Look at the diagram below, what else is produced during glycolysis? 2 ATP used up become 2 ADP 2 NADH & 2 H+ produced 4 ATP produced Net gain = 2 ATP Steps of Glycolysis: Step 1: breaks 1 molecule of glucose in half, producing 2 molecules of pyruvic acid (a 3-carbon compound) Step 2: 2 NAD+ ; electron carrier accepts 4 high-energy electrons transfers them to 2 NADH molecules and 2 H+ thus passing the energy stored in the glucose Step 3: 4 ADP added producing 4 ATP; net gain 2 ATP Step 4: 2 remaining pyruvic acids enter Krebs Cycle in presence of oxygen; IF no oxygen another pathway is followed 14. Glycolysis is a fast/ slow process. 15. Glycolysis alone ______________________ require oxygen. 16. What happens if a cell generates large amounts of ATP from glycolysis? 3 IV. Anaerobic to Aerobic Respiration 17. When was oxygen added to Earth’s atmosphere? ___________________________________________ 18. Why was this period known as the “oxygen catastrophe”? V. Mitochondrion Structure 19. Mitochondrion has two separate membranes: ______________ and ____________ membrane. 20. Three compartments: ________________________________, _________________________, and ______________. 21. Label the inner membrane, outer membrane, intermembrane space, cristae space, and matrix on the picture below: 22. What structure divides this organelle into three compartments? _______________________________ 23. Where is the intermembrane space located? 24. How is the cristae space formed? 25. What is the matrix enclosed by? What stage takes place here? 26. Where is the Electron Transport Chain located? 4 VI. Aerobic Respiration: The Krebs Cycle and Electron Transport Chain 27. Where is 90% of the unused chemical energy from glucose now stored? 28. How is this energy extracted? The Krebs Cycle: 2nd stage of Cellular Respiration 29. Here ______________________________ is broken down into ______________________________. 30. What is the first compound formed? __________________________________________ 31. What is another name for the Krebs Cycle? _____________________________________ Steps of the Krebs Cycle: (1) (2) (3) (4) (5) Pyruvic acid enters from glycolysis; One carbon removed = CO2 formed NAD+ again changed to NADH CoA joins remaining 2 carbons = Acetyl-CoA Acetyl-CoA added to 4 carbons to form = Citric acid (6-C) Citric acid broken down to 5-carbon, then 4 carbon; more CO2 released. Along the way more NADH and FADH2 formed and one molecule of ATP also made (6) 2 turns & 2 pyruvic acid (from glycolysis) yields: 10 NADH (2 from glycolysis) 2 FADH2 4 ATP (2 from glycolysis) (7) Cycle starts anew 5 VII. Uses for the products of the Krebs Cycle 32. ____________________________ is exhaled (waste product). 33. ________ can be used for cellular activities 34. High-energy electrons (stored in ________& __________) can be used to make huge amounts of ATP in the ____________________ of oxygen. VIII. Electron Transport Chain (1) Electrons from Krebs cycle are passed to electron transport chain by _________ & ___________ (2) At end of the chain an enzyme combines electrons from the electron chain with H + ions and oxygen to form ______________ (3) Each time 2 high-energy electrons transport down the electron chain, their energy is used to transport ______________across the membrane (4) H+ ions build up in __________________________________ it is now ___________________ charged, other side of membrane __________________ charged (5) Electrochemical gradient (chemiosmotic gradient) created for ____________________ to work (6) ATP synthase converts ________ into ________ 35. In __________________________, electron transport chain is a series of carrier proteins located in the inner membrane of the mitochondrion 36. In __________________________, same chain only in the cell membrane. 6 IX. The Total ATP production of Aerobic Cellular Respiration 7 X. Anaerobic Respiration: Fermentation 37. This occurs after glycolysis when oxygen is ________________________, thus anaerobic process. 38. In this process cells convert NADH to ________ by passing high-energy electrons back to pyruvic acid. 39. Now _____________________ has NAD+ and can continue producing ATP. 40. What are the 2 types of fermentation? ____________________________________________________________________________________ XI. Alcoholic Fermentation 41. What types of organisms go through alcoholic fermentation? 42. Look at the equation below and list the products of alcoholic fermentation: ____________________________________________________________________________________ pyruvic acid + NADH ethyl alcohol + CO2 + NAD+ XII. Lactic Acid Fermentation 43. Many cells convert accumulated pyruvic acid from gycolysis to lactic acid; lactic acid fermentation regenerates NAD+ so glycolysis can continue. 44. Look at the equation below and list the products of lactic acid fermentation: ____________________________________________________________________________________ pyruvic acid + NADH lactic acid + NAD+ 45. When your body cannot supply enough oxygen to muscle tissues during exercise to make ATP, how is energy production regenerated? 46. How do you get rid of the “BURN” sensation caused by the buildup of lactic acid in your body? XIII. Energy and Exercise 47. Initially body uses _______ which is already available in muscles; then new ATP made by _______________________________ and ____________________________________. 48. ATP in muscles only lasts a _________ seconds. 49. ATP from lactic acid fermentation lasts about ____ seconds 8 50. Exercise lasting longer than 90 seconds utilizes _________________________________ to generate a continuous supply of ATP. 51. Body stores energy in muscles and tissues in the form of _____________________ (carbohydrate); stores of glycogen usually lasts for ______________ minutes of activity, then the body starts to break down other molecules like __________ for energy. 52. HOW LONG DO YOU NEED TO ACTIVE BEFORE YOU START TO BURN FAT? XIV. Aerobic vs. Anaerobic Respiration: A Comparison 53. Advantages of Aerobic Respiration: a. ______________________________________________________________________________ b. ______________________________________________________________________________ 54. Advantages of Anaerobic Respiration: a. ______________________________________________________________________________ b. ______________________________________________________________________________ 9 XV. Relationship between Cellular Respiration and Photosynthesis Equation for Cellular Respiration: 6O2 + C6H12O6 6CO2 + 6H2O + energy (ATP) Equation for Photosynthesis: 6CO2 + 6H2O + energy (sunlight) 6O2 + C6H12O6 Photosynthesis Cellular Respiration ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ LET’S REVIEW… • How many stages does cellular respiration have? ___________ • What are the stages of cellular respiration? ____________________________________________________________________________________ Where does glycolysis take place? ____________________________________________________________________________________ Where does the Krebs cycle take place? ____________________________________________________________________________________ Where is the Electron Transport Chain located? • • • ____________________________________________________________________________________ • What do high-energy electrons help the cells build? _________________________________________ • What are the stage(s) of anaerobic respiration? ____________________________________________________________________________________ What are the stage(s) of aerobic respiration? • ____________________________________________________________________________________ • What are the two types of fermentation? ____________________________________________________________________________________ • Which fermentation process do humans use? ______________________________________________ 10 WORD BANK: Place the proper letter (a-n) in each circle of the flowchart. Every answer is used just once. a. b. c. d. e. f. g. h. i. j. k. l. m. n. 2 ATP 4 ATP 38 ATP 10 NADH 1 FADH2 Electron transport chain Mitochondrion Cytosol Fermentation Glycolysis Glucose 2 Pyruvate molecules Lactic acid Kreb's Cycle 11