Name _____________________________________ Period ______ Cell Cycle Internet Lab Date ______________ I. Use the following URL to complete the first part of the lab: http://biog-101-104.bio.cornell.edu/biog101_104/tutorials/cell_division.html Look at the Whitefish (animal cell) and Onion Root Tip (plant cell) Mitosis slides. Pay close attention to the descriptions of each of the phases of mitosis. Answer the six questions that follow. Question 1 ____________________________________ Question 2 ____________________________________ Question 3 ____________________________________ Question 4 ____________________________________ Question 5 ____________________________________ Question 6 ____________________________________ II. Use the following URL to complete the second part of the lab: http://www.biology.arizona.edu 1. Click on the Cell Biology link. 2. Click on The Cell Cycle and Mitosis link and read each of the three sections listed on the left of the screen. Click “Next” as you come to the end of each section. a.) DNA Basics b.) The Cell Cycle c.) Mitosis 3. Answer the following questions as you read each section. Not all answers are on the screen. You will have to recall information discussed in class notes for some questions. 1. Name the protein that DNA wraps around during chromosome formation. ____________________ 2. During which stage of the cell cycle does DNA replication occur? ____________________ 3. The nuclear membrane breaks down and centrioles begin moving to opposite sides of the cell during this phase. ____________________ 4. The centromere splits and replicated chromosomes separate during this phase. ____________________ 5. The nucleolus is present and chromosomes are not visible during this phase of the cell cycle. ____________________ 6. Chromosomes line up in the middle of the cell during this phase. ____________________ 7. The nuclear membrane reforms and spindle fibers break down during this phase. ____________________ 8. This is the longest phase of mitosis. ____________________ 9. This is the shortest phase of mitosis. ____________________ 4. Answer the following questions at the end of the Cell Cycle & Mitosis Tutorial 1) ______________________________________ 3) ______________________________________ 4) ______________________________________ 5) ______________________________________ 6) ______________________________________ 7) ______________________________________ 8) ______________________________________ 10) ______________________________________ 11) ______________________________________ 5. Next, complete the Online Onion Root Tips activity. Read the contents of the first two screens before beginning the activity. You are to place each drawing into one of the five phases. Keep tally marks in the table below of the number of cells you find in each stage. Then list what percentage of cells there are in each stage. Interphase Prophase Metaphase Anaphase Telophase Total 36 # of cells 100% % of cells % of cells calculation # of cells counted in each phase 36 X 100 = ______ III. Observe the prepared slides of mitosis in onion root cells and whitefish blastula. Locate the cells on the slide that are undergoing mitosis. In the observations section, draw and label what you observe (on high power)on the slide in the order that corresponds to the phases of mitosis. Observations: Onion Root Tip Whitefish Blastula Prophase Metaphase Anaphase Telophase