BIOL 235 - Microbiology I
Fall 2011
Lecture
Instructor
Mon 2:00-3:50 Professor C. Thompson
Crawford Hall
Room#103
Office
Office Hours
Phone
Email
247
CrfHl
Tues 11:00 – 1:00
Wed 1:00 – 2:50
Fri 11:00 - Noon
6136
thompsca@morrisville.edu
Tues 2:00-2:50
Crawford Hall
Room #107
COURSE DESCRIPTION: The biology of microscopic organisms including bacteria, fungi, protozoa, algae, and
viruses. An introduction to basic principles of microbiology, with emphasis on morphology, classification, cultivation,
growth, physical & chemical controlling agents, antibiotics, host-parasite interactions, and the benefits of
microorganisms including genetic engineering applications. The lab includes proper technique in the observation and
identification of microbes, and reactions under various physical and chemical conditions.
Prerequisite: Biology Placement BIOL 120 – General Biology I or Successful completion of college-level
biology (ex. BIOL 105) with at least a C-.
4 credits (3 lecture hours, 2 laboratory hours)
LAB: OSHA regulations for personal safety. If this is not adhered to you will be dismissed from lab.



NO FOOD or BEVERAGES of any kind.
Students MUST WEAR the provided LAB COAT
Students MUST WEAR safety goggles when instructed.
REQUIRED:
 Prescott’s Microbiology, 8th ed., 2011, Willey/Sherwood/Woolverton, McGraw Hill
 Companion Site Learning Center – Student Edition at www.mhhe.com/willey8
 Custom Lab Manual, Harley, McGraw-Hill
 Notebook to take notes in both lecture and lab
 3-ring notebook or binder
PROVIDED: Lab Coat - Safety Goggles- Microscope slides - Wax Marking pencil
LAP TOP COMPUTERS are not required for this course. In the event that a lab top computer is used any
inappropriate use will be dealt with on an individual basis.
CELL PHONES, iPODS, HEADSETS and other ELECTRONIC DEVICES: Prior to entering lecture or lab all
electronic devices must be turned off and put away. (unless you are recording the lecture to help you study).
Headsets are to be removed.
CIVILITY:
 Each student is expected to be courteous to fellow students and the instructor.
 There is to be no talking when the instructor is speaking or students are asking questions.
 Students are not to arbitrarily leave and come back to class during lecture or lab.
 When graded papers are passed back, students are to respectfully discuss any questions privately with the
instructor.
TUTORS: No tutors are available during the summer session.
STUDENTS WITH DISABILITIES: Please contact Mr. David Symonds, at the AEC in the Library, to discuss
special accommodations. No special accommodations will be provided until the instructor has been notified by Mr.
Symonds.
ATTENDANCE POLICY:
According to the Faculty & Staff Handbook of Morrisville State College, each student is expected to attend all
scheduled classes and laboratories.
Lecture

Each TUESAY there will be either a quiz or an exam. Quizzes will be administered towards the end of
class.

Students may not come to lecture late to just take a quiz; if so it will be counted as a zero.

If an absence is unavoidable, it is the student's responsibility to obtain the material (notes) missed.

If an absence is unavoidable on an exam day you are to contact the instructor by 5:00 pm that day to
make arrangements to take the exam, before it is passed back.

There are NO make-ups for any missed quizzes. One lecture quiz will be dropped to accommodate an
unavoidable absence.
Lab

Students may not come to LAB to just take a quiz and leave; if so it will be counted as a zero.

Students are not to leave lab when instructions are given.

If an absence in LAB is unavoidable, it is the student's responsibility to notify the instructor immediately
so arrangements can be made to attend another lab section. You may NOT make up more than two (2)
labs during the semester this way.
LAB Sections
01L
02L
03L
Room
Crawford Hall #257
Crawford Hall #257
Crawford Hall #257
Day
Monday
Tuesday
Wednesday
Time
4:00 – 5:50 p.m.
3:00 – 4:50 p.m.
3:00 – 4:50 p.m.
 The lowest lab quiz and lab report grade will be dropped at to compensate for an unavoidable absence.

If you miss a week’s lab you are responsible for reading over the lab missed and ask any questions
beforehand in preparation for the quiz that will be administered the following week.

If you miss a week’s lab you are to submit the lab report that was due during your absence to your lab
instructor by the next lab period.

Papers submitted without a name will receive a zero

You must be in lab and perform the lab experiment in order to turn in the lab report for that exercise.
ACADEMIC HONESTY:

Keep in mind that assignments are given to allow you to demonstrate what you have learned in class as well
as show off your own creativity.

Each assignment is therefore expected to be your own original work.

Any work that is determined by the instructor to be dishonest in any form will be treated according to the
standards for Academic Honesty as described in Morrisville State College Student Handbook and
Faculty/Staff Handbook
Academic dishonesty
From Wikipedia, the free encyclopedia
Fabrication is the falsification of data, information, or citations in any formal academic exercise. This includes
making up citations to back up arguments or inventing quotations. Fabrication predominates in the natural
sciences, where students sometimes falsify data to make experiments "work". It includes data falsification, in
which false claims are made about research performed, including selective submitting of results to exclude
inconvenient data to generating bogus data.
There is also the practice of dry-labbing—which can occur in chemistry or other lab courses, in which the
teacher clearly expects the experiment to yield certain results (which confirm established laws), so the student
starts from the results and works backward, calculating what the experimental data should be, often adding
variation to the data. In some cases, the lab report is written before the experiment is conducted—in some cases,
the experiment is never carried out. In either case, the results are what the instructor expects.
Deception is providing false information to a teacher/instructor concerning a formal academic exercise.
Examples of this include taking more time on a take-home test than is allowed, giving a dishonest excuse when
asking for a deadline extension, or falsely claiming to have submitted work. This type of academic misconduct
is often considered softer than the more obvious forms of cheating, and otherwise-honest students sometimes
engage in this type of dishonesty without considering themselves cheaters. It is also sometimes done by students
who have failed to complete an assignment, to avoid responsibility for doing so.
Cheating can take the form of crib notes, looking over someone's shoulder during an exam, or any forbidden
sharing of information between students regarding an exam or exercise. Many elaborate methods of cheating
have been developed over the years. For instance, students have been documented hiding notes in the bathroom
toilet tank, in the brims of their baseball caps, or up their sleeves. Also, the storing of information in graphing
calculators, pagers, cell phones, and other electronic devices has cropped up since the information revolution
began. While students have long surreptitiously scanned the tests of those seated near them, some students
actively try to aid those who are trying to cheat. Methods of secretly signaling the right answer to friends are
quite varied, ranging from coded sneezes or pencil tapping to high-pitched noises beyond the hearing range of
most teachers. Some students have been known to use more elaborate means, such as using a system of
repetitive body signals like hand movements or foot jerking to distribute answers (i.e. where a tap of the foot
could correspond to answer "A", two taps for answer "B", and so on).
Cheating differs from most other forms of academic dishonesty, in that people can engage in it without
benefiting themselves academically at all. For example, a student who illicitly telegraphed answers to a friend
during a test would be cheating, even though the student's own work is in no way affected. Another example of
academic dishonesty is a dialogue between students in the same class but in two different time periods, both of
which a test is scheduled for that day. If the student in the earlier time period informs the other student in the
later period about the test; that is considered academic dishonesty, even though the first student has not
benefited himself.
Impersonation is a form of cheating whereby a different person than the student assigned an assignment or
exam completes it. Unlike in Cheating, the academic work is totally 'outsourced' to another person or
organization, usually for pay
GRADING POLICY:





Grades are NOT negotiable.
There is NO extra credit; Grades are based on quality not quantity.
Grades are not curved.
Students may not attend Lab or Lecture to just take a quiz and leave (if so it will be counted as a zero).
Grades are EARNED by the quality of knowledge you learned from lab and lecture as demonstrated
by quizzes, exams, and lab reports.
% of Lecture Grade
Quizzes
30
Exams
45
Final Exam 25
% of Lab grade
Lab Reports
Quizzes
Unknown
30
45
25
Lecture – 75% of course grade:
Your lecture grade will comprise 75% of your BIOL 235 course grade and will be computed by taking 75%
of your exam average, and 25% of your final exam grade. These values together to give you a numerical
grade for lecture, which is then multiplied by 0.75 (75%) and added to 25% of your lab grade.
Example:
Quiz average 80 x 0.30 = 24.00
Exam average 75 x 0.45 = 33.75
Final exam
70 x 0.25 = 17.50
75.25 = C+
Lab – 25% of course grade:
Your lab grade will comprise 25% of your BIOL 235 course grade and will be computed by taking 30 % of
your lab report average, 45% of your quiz average, and 25% of your grade earned on the determination of an
unknown bacterial culture. These values together to give you a numerical grade for lab, which is then
multiplied by 0.25 (25%) and added to 75% of your lecture grade.
Example:
Lab Quiz average
Lab report average
Unknown
75 x 0.45 = 33.8
85 x 0.30 = 25.5
100 x 0.25 = 25.0
84.3 x 0.25 = 21.1 + 55.4 = 76.5 = C+
The following scale will be applied in determining your final letter grade.
 A grade of “D” is a passing grade, but will not transfer.
 Grades are rounded to the next letter grade (e.g. 92.5 is rounded to a 93 “A”; a 92.4 remains a 92 “A-“)
93 - 100%
89 - 92
85 - 88
82 - 84
78 - 81
74 - 77
70 – 73
66 - 69
63 - 65
60 - 62
0 –59
A
AB+
B
BC+
C
CD+
D
F
BIOL 235 - STUDY TIPS:
 Listen carefully in class and lab. You may record the class.
 Ask questions when you don’t understand something
 Take notes, and do so in an organized fashion. Make sure the information is organized
with general headings and sub-headings. You may want to rewrite/reorganize the notes
you take in class
 Make sure you write down definitions correctly; it may help you to use flash cards.
 Underline, circle, or highlight important points in your lecture notes, your textbook, and in
your lab manual.
 When reading the text, study the diagrams to help you visualize concepts.
 Review the chapter summaries; they are there to help you.
 Read over the lab exercise ahead of time.
 Don't stay up all night cramming for an exam or quiz. Instead, organize your study time
into small realistic blocks of study time through out the week.
 To study for exams, review the material first using your notes, any handouts, and the text
book. Then review any quizzes on the material to self-test yourself.
 Use the on-line Companion Site Learning Center “Student Edition” at
www.mhhe.com/willey8 for on-line Flash cards, Animations, Self testing etc by chapter.
For every hour of class you should be studying at least 2 hours a week, therefore,
you should be studying at least 10 hours a week for this course.
Fall 2011
BIOL 235 – Tentative Lecture Schedule
Each TUESDAY there will be either a quiz or an exam.
Quizzes will be administered towards the end of class.
A Brief Overview covered throughout the semester of Ch.#30-31: Microbial Interactions, Infection & Pathogenicity
Ch.#32-33: Nonspecific & Specific Immunity, and Ch.#36 – Epidemiology & Public Health Microbiology
CHAPTER – TOPIC
WEEK
1
Aug 22-23
Orientation; Ch.#1 – Evolution of Microorganisms and Microbiology
2
Aug 29-30
Ch.#1 – Evolution of Microorganisms and Microbiology Continued
Ch.#3 – Bacteria & Archaea Cell Structure & Function
3
Sept 5-6
4
Sept 12-13
Ch.#4 – Eukaryotic Cell Structure & Function
Ch.#6 – Microbial Nutrition
5
Sept 19-20
Ch.#6 – Microbial Nutrition Continued
Ch.#7 – Microbial Growth
6
Sept 26-27
Ch.#7 – Microbial Growth Continued
Ch.#9 – Introduction to Metabolism (Enzymes)
EXAM #2
7
Oct 3-4
October 10-11
Oct 17-18
8
Ch.#3 – Bacteria & Archaea Cell Structure & Function Continued
EXAM #1
Ch.#10 – Catabolism: Energy Release and Conservation (Respiration)(Fermentation)
Fall Break
Ch.#11 – Anabolism: The Use of Energy & Biosynthesis (Photosynthesis)
Ch.#12 – Genes: Structure, Replication & Expression (Protein Synthesis)
9
Oct 24-25
Ch.#12 – Genes: Structure, Replication & Expression (Protein Synthesis) Continued
Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Mutations)
EXAM #3
10
Oct 31-Nov 1
Ch.#5 – Viruses and other Acellular Infectious Agents Structure & Function
Ch.#25 – The Viruses
Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Bacterial Genetics)
11
Nov 7-8
12
Nov 14-15
Ch.#19-22 Bacteria Continued
Ch.#23 – The Protists
EXAM #4
13
Nov 21-22
Ch.#23 – The Protists Continued
Ch.#24 – The Fungi
14
Nov 28-29
Ch.#8 – Control of Microorganisms in the Environment
EXAM #5
15
16
Dec 6-9
TBA
Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Bacterial Genetics) Continued
Ch.#15 – Recombinant DNA Technology
Ch.#18- The Archaea
Ch.#19-22 Bacteria
Ch.#34- Antimicrobial Chemotherapy
Comprehensive Final Exam
Fall 2011
BIOL235 – Tentative Lab Schedule
Crawford Hall #257
01L Monday 4:00-5:50
02L Tuesday 3:00-4:50
03L Wednesday 3:00-4:50
LAB OPEN
to check lab results after 24-48 hour incubation period
Tuesday
9:00 am – 1:50 pm
Wednesday 10:00 am – 2:50 pm
Friday
10:00 am – Noon
WEEK OF
REPORT DUE
QUIZ
PERFORMING
Orientation & Lab Safety
Ex.#1- Bright-field Microscope
1
Aug 22
2
Aug 29
Ex. 1
Ex. 1
Ex.#7 - Smear Preparation & Simple Staining
3
Sept 5
Ex. 7
Ex. 7
Ex.#8 - Gram Staining
4
Sept 12
Ex. 8
Ex. 8
Gram Stain Quiz Part A: Written Quiz
Part B: Interpretation of Gram Stained Slide
5
Sept 19
6
Sept 26
Ex. 9-10
Ex.9-10
7
Oct 3
Ex. 16
Ex.16
8
October 12
03L Only
Ex.20,22, 23
Ex.20,22,23
Ex.#42- Effects of Chemical Agents on Bacteria I: Disinfectants
and other antimicrobial products
9
Oct 17
Ex.20,22, 23
03L Ex.42
Ex.20,22,23
Ex.42
Ex.#24 - Proteins, Amino Acids & Enzymes I: H2S & Motility
Ex.#25 – Proteins, Amino Acids & Enzymes II: IMViC Tests
10
Oct 24
Ex. 24-25
Ex.24-25
Ex.#26 - Proteins, Amino Acids & Enzymes III- Casein Hydrolysis
Ex.#28 – Proteins, Amino Acids & Enzymes V: Catalase Activity
10
Oct 31
Ex. 26 & 28
Ex. 26 & 28
11
Nov 7
Ex. 30,31,34
Ex. 30,31,34
Ex.#30 - Proteins, Amino Acids & Enzymes VII: Oxidase Test
Ex.#31 - Proteins, Amino Acids & Enzymes VIII: Urease Activity
Ex.#34 - Proteins, Amino Acids & Enzymes XI: Nitrate Reduction
Ex.#43- Effects of Chemical Agents on Bacteria II: Antimicrobial
Agents (Kirby-Bauer Method)
12
Nov 14
Ex. 43
Ex. 43
13
Nov 21
01L & 02L
Only
Parasitology
Parasitology
14
Nov 28
15
Dec 5
Ex.#9 - Acid-Fast Staining (Ziehl-Neelsen)
Ex.#10 – Endo-spore Staining (Schaeffer-Fulton)
Ex. 42
Ex. 42
03L Parasitiology
Parasitiology
Unknown
Ex.#16 - Streak-Plate Method& Differential Culture Media
Ex.#20 - Carbohydrates Fermentation I
Ex.#22 – Carbohydrate Fermentation III
Ex.#23 - Lipids: Lipid Hydrolysis
Handout – Introduction to Parasitology
Ex.#42- Effects of Chemical Agents on Bacteria I: Disinfectants
and other antimicrobial products
UNKNOWN
Unknown Due; Clean-up; Review for Final Exam
SAFETY RULES IN THE MICROBIOLOGY LABORATORY
1.
ONLY microbiology students are allowed in the laboratory.
2.
NO EATING, DRINKING, GUM CHEWING, is permitted in the laboratory. Do not bring food or
beverages into the lab. If this is not adhered to you will be dismissed from lab.
3.
Do not remove cultures, reagents, or other materials from the laboratory at any time.
4.
Lab coats are to be worn at all times in the laboratory. This helps to ensure that no culture material is
accidentally deposited on your clothes or skin, and protects you and your clothes from spillage of
chemicals and stains.
5.
Jackets/coats are to be hung up on the hooks located on the back wall underneath the windows.
6.
All other items, such as books, and bags, should be stored under your work area. Only those
materials pertinent to your lab work, such as the lab manual and a notebook, should be brought to your
laboratory workspace.
7.
Note the location and use of the safety eyewash station, safety shower, fire blanket, and first aid
kit.
8.
Minimize hand to face contact and avoid putting pencils or pens in the mouth.
9.
Begin each laboratory session by disinfecting your work area. Saturate the area with disinfectant
using a paper towel, and allow the area to dry. Repeat this procedure after you have finished your work
to ensure that any material you have deposited on the work surface is properly disinfected.
10.
Labeling is critical to avoid improper use or disposal of material. All culture material and chemicals
are to be properly labeled with your name, laboratory section, date, and exercise.
11.
Long hair must be secured in a ponytail. Be very careful with Bunsen burners. To avoid injuries,
burners should be turned off when not in use. When reaching for objects, be careful not to place your
hands into the flame.
12.
Sterilize the inoculating loop and/or needle immediately BEFORE and AFTER each use and keep
them in an upright position in the block holder provided.
13.
Keep culture tubes in an upright position in the test tube rack at all times. Never lay a test tube flat on
the workbench.
14.
Contaminated material/glassware are not to be thrown in the trash cans. All contaminated material
must be disposed of properly. Used swabs, pipettes, and disposable Petri dishes are placed in the red
biohazard bag provided. Contaminated reusable glassware is to be placed on the dirty glassware cart
located in the front of the room.
15.
Clean up after yourself. Keep sink areas clean, and keep your microscope clean.
16.
Wash your hands before leaving the lab.
17.
In case of any accident or injury, report immediately to the laboratory instructor so that prompt
and proper action can be taken. This includes burns, cuts and spills no matter how minor they are.
18.
When “live” cultures are spilled notify the instructor immediately. Use paper towels to cover and
contain the spread of the spill. Soak the towels with disinfectant. Allow 15 minutes for the disinfectant
to react with the spilled culture. Use dry paper towels to pick up the wet towels and aseptically transfer
the contaminated materials to the red biohazard bag provided.