SAMPSON COMMUNITY COLLEGE Course Syllabus for Microbiology (Biology 275) Instructor: Nahel W. Awadallah Office: Transfer Building, Rm# 220 Office Hours: Monday & Wednesday 010:30am - 12:30pm Phone #: (910) 592-8081, Ext. 5511 Web Pages: http://biology.sampson.cc.nc.us & Http://science.sampson.cc.nc.us E-mail address: nawadallah@sampsoncc.edu --------------------------------------------------------------------------------- 1. Course Prefix and No. BIO 275 Title: Microbiology 2. Credit Hours Contact Hours: 8 6 Lecture Hours 4 Shop Hours 0 Clinical Hours 0 Lab Hours 4 3. Proficiency Exam YES NO X If YES, see Chairman of ________________________________ 4. Prerequisite(s): Course Prefix and No. BIO 110 or BIO 168 1 5. Corequisite(s): Course Prefix and No. _________ 6. Title: __________ Course Description: This course covers principles of microbiology and the impact these organisms have on man and the environment. Topics include the various groups of microorganisms, their structure, physiology, genetics, microbial pathogenicity, infectious diseases, immunology, and selected practical applications. Upon completion, students should be able to demonstrate knowledge and skills including microscopy, aseptic technique, staining, culture methods, and identification of microorganisms. This course has been approved to satisfy the Comprehensive Articulation Agreement pre-major and/or elective course requirement. EFFECTIVE DATE: SPRING 2004 Form CAC 2 7. Course Goals and Purposes: The student will develop and demonstrate an understanding of the basic concepts of Microbiology. 8. Teaching Facilities and Resources: Classroom Laboratory Audio-visual Aids Library Instructional Support Center 9. Number of Personnel Required Instructors 1 Lab Assistants 1 Other 0 10. Required Textbooks and Workbooks: 1. Microbiology- Principles and Explorations by Jacquelyn G. Black, 5th addition 2. Laboratory Exercises in Microbiology by Robert A. Pollack, Walter Mondschein, Lorraine Findlay, and R. Ronald Modesto. 2 11. Required Clothing, Materials, Supplies, etc. 12. Attendance & Tardy Policy: Attendance Policy All students are expected to attend every scheduled class. Attendance is computed beginning with the first scheduled class meeting. If an emergency prevents a student from attending class, the student should notify his/her instructor as soon as possible. In the event that a student does not attend at least 85 % percent of all scheduled contact hours, the instructor may drop the student. If there are extenuating circumstances, the instructor has the authority to make an exception to the attendance policy. Students are expected to report to class on time. Specific guidelines regarding attendance and tardiness are included in course outlines. Tardy Policy Students are expected to attend class on time and remain for the duration of the class. Late arrivals an/or early departures will count toward total absences. Two tardies or two early departures will count as one absence. Students whose persistent tardiness disrupts class instruction may be dropped from the course at the discretion of the instructor. 13. Safety Rules: All students must preview a slide presentation entitled "Laboratory Safety: Protecting Yourself and Others" Learn the proper escape routes in case of fire or explosion. Learn the location and operation of the following safety equipment: 1. 2. 3. 4. eye wash safety shower fire blanket fire extinguisher 5. acid and base spill clean-up kit 6. fume hood 7. exhaust fan 14. Classroom, Shop, Clinical, or Lab Rules and Guidelines: NO Smoking No Playing Around NO Eating or Drinking Students with Disabilities Students with documented disabilities will be afforded accommodations in their classes 3 consistent with the requirements of the Americans with Disabilities Act. You should notify your instructor within the first week of classes if you have a disability for which you request assistance. Additional information man be obtained from Student Support Services, in Warren 215, ext. 3511. Please understand that the college is unable to provide you any accommodation unless you provide professional documentation of your disability and a timely notification of your needs. 15. Methods of Student Evaluation (Examples: Lab Returns, Term Paper, Transcription, Exam, Timed Writings, Pop Tests, Projects) The final grade will be determined in accordance with the following scale: 90 - 100 = A 80 - 89 = B 70 - 79 = C 60 - 69 = D 0 - 59 = F Inventories will be composed of objective and subjective questions. 16. Exit Criteria (Outcomes Assessment) The student will develop and demonstrate an understanding of basic principles underlying the science of microbiology. The cumulative grade average cumulative grade average must be a minimum of 70 in order to earn the minimum passing grade of a C. Final assessment will be based on the average of all grades received during the quarter. 17. Table of Contents (Course Content) (See Unit Titles) Chapter 1 The scope and history of microbiology Chapter 3 microscopy and staining Chapter 4 Characteristics of Procaryotic & Eucaryotic Cells Inventory I Chapter 5 Essential concepts of Metabolism Chapter 6 Microbial Growth and culture Chapter 7 Microbial Genetics Chapter 8 Gene transfer and genetic engineering Inventory II Chapter 9 Classification of Microorganisms Chapter 10 VIRUSES Chapter 11 Eukaryotic microorganisms and Parasites 4 Chapter 12 Sterilization and disinfections Inventory III Chapter 13 Antimicrobial therapy Chapter 14 Host-microb interaction and Mechanisms of Pathogenicity Chapter 15 Epidemiolgy and nosocomial infection Chapter 16 Nonspecific Defenses of the Host Chapter 17 Immunolgy I : Basic principles Chapter 18 Immunolgy II: Practical Applications of Immunology Chapter 19 diseases of the skin and eye; wounds and bites. Chapter 20 Urogenital and sexually transmitted diseases Inventory IV Chapter 21 Diseases of the Respiratory System Chapter 22 Oral and gastrointestinal diseases Chapter 23 Cardiovascular, lymphatic and systemic diseases Inventory V Chapter 24 Diseases of the nervous system Chapter 25 Environmental microbiology Chapter 26 Applied microbiology Inventory VI Laboratory Exercises and Experiments: 1. The Microscope 2. Handling and Examing Cultures 3. The Handling Drop Preparation 4. Simple Strains 5. Gram Strain 6. Acid Fast Strain 8. Culture Media 9. Pure Culture Technique 10. Pour Plate and Subculture Techniques 11. Culturing Microorganisms from the Environment 12. Moist and Dry Heat 13. The Autoclave 5 14. Disinfectants 15. Antibiotics (Antibiotic Susceptibility Testing) 16. Primary Media for Isolation of Microorganisms 17. Some Metabolic Activities of Bacteria 18. Activities of Bacterial Enzymes 19. Streptococci Pneumococci, Enterococci 20. Staphylococci 23. Clinical Specimens from the Respiratory Tract 25. Clinical Specimens from the Intestinal Tract 26. Urine Culture Specimens 27. Neisseria and Spirochetes 28. Anaerobic Bacteria 18. Course Content Outline and Behavioral Objectives See Attached 19. Class Handouts 20. Supplemental Bibliography of reference materials and audio visual aids used in this course. TITLE OF UNIT: OBJECTIVES: APPROXIMATE TIME: CONTENT AND COMPETENCIES The student should be able to: As a result of the experiences provided by the lectures and experiments in this course students will have the opportunity to accomplish successfully following objectives. 1. Define microorganisms. 2. Enumerate the characteristics of prokaxyotic and eukaryotic cells. 3. Label a compound microscope and list the functions of each part. 4. List the main types of laboratory equipment 6 and how each is employed in a microbiology laboratory. 5. Differentiate between positive and negative stained organisms. 6. List the procedure for simple and gram stains. 7. List the major types of culture media. 8. List the three major morphological shapes of bacteria. 9. Draw and label a flagella. 10. Draw and label a cell wall. 11. List the stages of endospore formation. 12. List the components of DNA. 13. Define transcription and translation. 14. Define mutation processes. 15. Differentiate between transformation, transduction and conjugation. 16. Define and give examples of genetic engineering and recombinant DNA. 17. List the characteristics and culture techniques of Rickettsial, Chlamigdial, Mycoplasmas, Bdellovibios, and Actinomycetes. 18. Enumerate virus characteristics. 19. List the stages of lytic and lysogenic cycles. 20. List the concepts of oncogenes in human cancer. 21. List the major groups of eukaryotic organisms. 22. Enumerate the major physical methods for the control of microorganisms. 23. Enumerate the major chemical agents for the control of microorganisms. 24. Diagram the major factors influencing nosocomial infections. 25. Enumerate the major types of chemotherapeutic agents and mode of action of each agent on microbes. 26. Categorize the major kinds of infectious diseases. 27. Diagram invasive mechanisms of bacteria. 28. List examples of mechanical and chemical host resistance. 29. Diagram the process of phagocytosis and inflammation. 30. Define interferons role in resistance. 31. List the properties of antigens and antibodies. 32. Differentiate B cells and T cells. 33. Differentiate active and passive immunity. 34. Define delayed-type hypersensitivity. 35. List the major types of amteseria and vaccines. 36. Define epidemiology. 37. Enumerate the normal flora of the human body. 38. Enumerate the major types of bacteria that enter the body via the respiratory tract. 39. Enumerate the viruses, chlamydiae, and Rickettsiae microbes that enter the body via the respiratory tract. 40. List the pathogens associated with gastrointestinal disease. 41. Enumerate the pathogens that enter the body via the genitourinary tract. 7 42. Enumerate the pathogens that enter the body via the skin. 43. Enumerate the pathogens that enter the body via anthropod bites. 8