Experiment 1 Warming Function of Nasal Passageways Nasal passageways transport air to and from the lungs and also serve to warm and humidify this air. The nasal turbinates provide a large surface area and rich blood supply for a process of heat exchange which contributes to the maintenance of a constant body temperature (see Figure 1). Warming of inhaled air improves lung function and removal of heat from the exhaled air helps to prevent excessive heat loss from the body. Conditions such as viral infections, allergies, and deviated septae can all lead to plugging of nasal passageways and mouth breathing. Inhaling colder air through the mouth can irritate the lungs and worsen conditions such as bronchitis and asthma. Front view Side view Figure 1 In this experiment, you will use the Vernier Surface Temperature Sensor to measure the temperature of exhaled air. Air that has passed through the nasal passageways will be compared with air that was inhaled through the mouth. In both cases, the air spends time in the lungs where heat is acquired from exposure to the extensive lung tissue alveolar surface area (see Figure 2). However, air that has passed through the nasal passageways arrives at the lungs already partially warmed. Figure 2 Human Physiology with Vernier 1-1 Experiment 1 OBJECTIVES In this experiment, you will Compare the temperature of air that has passed through the nasal passageways with air that has not. Evaluate the contribution of nasal passages vs. lungs to the warming of the air we breathe. MATERIALS computer Vernier computer interface Logger Pro Vernier Surface Temperature Sensor plastic straws tape PROCEDURE Comparison of Temperature of Air Inhaled Through Nose and Air Inhaled Through Mouth. 1. Connect the Surface Temperature Sensor to the Vernier computer interface. Open the file “01 Warming Function” from the Human Physiology with Vernier folder. 2. Insert the end of the Surface Temperature Sensor in one end of a plastic straw such that the tip of the sensor is approximately 2 cm into the straw. Fold the wire back over the straw and affix it to the outside of the straw with tape (see Figure 3). 3. Click to begin data collection. Record a baseline temperature for approximately 5 s or until a stable baseline temperature is reached. Figure 3 4. After the baseline temperature has stabilized, begin taking normal breaths through the nose and exhaling by blowing your breath out the mouth and through the straw (approximately 2–3 s per inhalation and 2–3 s per exhalation). Remove the straw from the mouth after each exhalation. Continue this process until data collection is complete. Data will be collected for 40 s. 5. Store this run by choosing Store Latest Run from the Experiment menu. 6. Click to establish a temperature baseline. If the temperature is higher than the baseline temperature of the first run, allow the baseline temperature to fall to a level close to what it was at the start of the first run. You may speed this process by inhaling and/or blowing through the straw. Once your baseline temperature has been reached, click . 7. Click to begin data collection. Record a baseline temperature for about 5 s. 8. After the baseline temperature has stabilized, begin taking normal breaths through the mouth and exhaling through the straw (approximately 2–3 s per inhalation and 2–3 s per exhalation). Use a nose clip or pinch the nose closed to prevent accidental nose breathing. Remove the straw from the mouth during each inhalation. Continue this process until data collection is complete. Data will be collected for 40 s. 9. Click the Statistics button, . Check the boxes in front of Latest and Run 1, then click . Record the maximum and minimum temperatures to the nearest 0.1°C for each run in Table 1. 1-2 Human Physiology with Vernier Warming Function of Nasal Passageways 10. Subtract minimum from maximum values to obtain the temperature change and record these values as ∆t. Then, subtract the ∆t values for mouth inhalation (Latest) from nose inhalation (Run 1) and record this value in the column labeled ∆t Run 1 – ∆t Latest. DATA Table 1 Run 1 (nose inhalation) Latest (mouth inhalation) ∆tRun 1 – ∆tLatest Maximum temperature (°C) Minimum temperature (°C) ∆t (°C) DATA ANALYSIS 1. Reviewing your data, what conclusions can you reach regarding where inhaled air receives most of its heat? 2. What proportion of the difference in temperature between inhaled and exhaled air can be attributed to the nasal passageways when nose breathing? Assume that the warming contribution of the mouth is negligible. (For the denominator, use the ∆t value for Run 1 in Table 1). 3. The average surface area of the nasal passageways is 160 cm2 and the average surface area of the alveoli in the lungs is approximately 100 m2 (1 106 cm2). Use the data you collected to calculate the amount of heat contribution per unit area of (a) nasal epithelium surface area to which the air is exposed. (b) alveolar surface area to which the air is exposed. In both cases, ignore confounding variables such as the volume of air that stays in the bronchi (also called dead space) and the volume of air that does not leave the lungs, also called functional residual capacity. 4. Describe a situation in which it would be beneficial to breathe through the mouth. Human Physiology with Vernier 1-3