Jordan Miller
Miss Merva: Formal Lab Report
Biology: 2nd Block
3/8/2016
Blood Test Lab Formal Lab Report
Introduction
There are three different types of blood that can circulate throughout human body
on the red blood cells; type A, type B, and type O, all of which can have a
positive or negative presence of the Rh protein on the cell. This ABO
classification system was discovered by Karl Landsteiner in 1901. Today there
are over thirty blood groups that have evolved from this system creating the
ability to do investigation such as paternity testing. Paternity testing is basically
what the Glee lab consisted of. In short, there was a love triangle between Quinn,
Finn and Puck. Quinn winded up pregnant and doesn’t know who to blame, her
boyfriend Finn, or bad boy Puck. The hypothesis for our group was that if the
baby’s results from the blood test given were the same as Puck’s, then the baby
would have to be his unless Finn had the same blood type because their blood
types would match.
Experiment
Materials:

Blood type slide

10 toothpicks

Anti-A serum

Anti-B serum
Jordan Miller
Miss Merva: Formal Lab Report
Biology: 2nd Block
3/8/2016


Anti-Rh serum
4 blood samples
Procedure: For this experiment, four single blood tests were performed, one
on Quinn, one on Finn, one on Puck, and one on the baby. From there, the
four samples underwent testing for A and B blood types, assuming that it
would be O if neither. An Rh test was also run on all the blood samples to
determine if it was positive or negative for that protein. This was done by
taking all the designated A samples and mixing it with a drop of an Anti-A
serum and so forth for the B and Rh tests with their anti serums. The goal
was to see which blood samples clot or clump up based on their exposure to
the different serums.
Results
Sample
#
Person
1
Quinn
2
Puck
3
Finn
4
Baby
Agglutination (+ or -)
A
B
Rh
+
+
+
+
+
+
_
(none)
+
ABO Blood
Type
A+
B+
ABO+
Rh Blood
Type
Possible genotypes
+
IA IA, IA i
+
IB IB, IB i
_
IA IB
ii
Observations: During the experiment, it was noted that none of the potential
parents had the same blood type which gave our group the ability right then in
there to determine who the father of the baby was. As showed in the table,
Quinn’s blood was found A+ because it clotted in the blood type A and Rh tests.
Jordan Miller
Miss Merva: Formal Lab Report
Biology: 2nd Block
3/8/2016
Puck’s blood was B+ because it clotted in the blood type B and Rh tests. Finn
was AB- because his blood clotted in both A and B blood tests but not in the Rh
test. Finally, the baby’s blood type was O+ meaning that it had neither A or B
blood type but did have Rh. Therefore the father of Quinn’s child could not have
been Finn because he could have only given the child A or B blood based on the
possible genotypes. Puck on the other could have been the father because one
of his possible genotypes is IB i and one of Quinn’s was IA i, making them the only
possible combination of parents to have the child. They are the only ones with a
recessive (i) in their possible genotypes giving the baby both recessive genes
and resulting in a type O blooded baby.
Calculations
The results were calculated by observing which blood types clotted when mixed
with different serums, as displayed in the chart
below. The plain red circles represent no clotting
where as the speckled circles demonstrate clotting
or positive tests for that blood type.
Conclusion
In conclusion Puck is the only possible father to the
child out of the possibilities given. This was found
through the blood testing by seeing that Puck was the only male that could have
given the baby a recessive gene because of his possible heterozygous B+ blood
type. When mixed with Quinn’s potential heterozygous A+ blood type, the baby
Jordan Miller
Miss Merva: Formal Lab Report
Biology: 2nd Block
3/8/2016
could receive both recessive genes causing it to have O+ blood where as if Finn
were the father, there would be absolutely no chances of the baby having O+
blood due to him only having one blood type option, IA IB. The results agreed with
our hypothesis because Puck indeed was the father of Quinn Fabray’s baby
however we would probably want to test the blood more than once next time to
insure there being no room for error with the different serums. In the end
however, the test did give a definite result.
References:
http://nobelprize.org/nobel_prizes/medicine/laureates/1930/landsteinerbio.html
http://en.wikipedia.org/wiki/Blood_type#History