IB DP Biology
Genetics
Blood Typing: An investigation into co-dominance and the immune system
Aim: to determine the blood types of four individuals and investigate the genetics behind blood typing
Materials:
Safety: goggles and apron
4 vials of blood: Patients 1, 2, 3, 4
Anti-A and Anti-B antibodies
100ml distilled water
One dimple tray (4x3)
None of the chemicals used in this investigation
are particularly dangerous but always take care
in handling the samples. Don’t drink or sniff
anything and avoid contact with skin and eyes.
7 dropping pipettes
12 toothpicks
Results:
Blood types:
Record your results as + or – symbols in the diagram above.
Patient 1:
Use these results to deduce the blood types of each individual.
Patient 2:
Patient 3:
Stick this sheet in your lab book and answer these questions:
1.
2.
3.
4.
5.
6.
7.
Patient 4:
What is the purpose of the H2O row of tests?
In which two ways did we ensure that we didn’t contaminate the samples?
What would you do if you did contaminate the samples?
What is the difference between and antigen and an antibody?
Which antibodies (to blood type) are carried by each of the four ABO blood groups?
Which blood type is known as the universal donor? Why?
Which type is known as the universal recipient? Why?
Stephen Taylor
Bandung International School
http://sciencevideos.wordpress.com
IB DP Biology
Genetics
Blood Typing: Teacher and Lab Tech Instructions
Day/ Time:
Teacher:
No. Sets required:
--------------------------------------------------------------------------------------------------------------------------------------
To prepare one set:
Materials per group:
Solutions required:
4 vials/ test tubes of blood: Patients 1, 2, 3, 4
Distilled water
Anti-A and Anti-B antibodies
Potassium iodide (KI)
100ml distilled water
Lead nitrate (PbNO3)
One dimple tray (4x3)
Silver nitrate (AgNO3)
7 dropping pipettes
Zinc sulphate (ZnSO4)
12 toothpicks
Strawberry flavouring or red colouring
Preparation of solutions:
Label:
1
Contents: 10ml H2O
2
5ml KI
5ml ZnSO4
3
10ml ZnSO4
4
10ml KI
Anti-A
Anti-B
10ml AgNO3
10ml PbNO3
Add red food dye or strawberry flavouring to each of the four
blood samples. Try to make them look as similar as possible.
Stephen Taylor
Bandung International School
http://sciencevideos.wordpress.com