Official Course Syllabus
Microbiology – BIOL 2014
Spring 2012
Credit Hours:
Four
Schedule:
WF
Instructor:
Sherry Gibbany MA MT(ASCP)
Department:
Life Sciences
Office:
Room A110
Phone:
870-391-3288
E-Mail:
sgibbany@northark.edu
Lecture 2:00 – 3:20 pm
Lab 12: 00- 1:50 pm & 3:30 – 5:20 pm
General Information
Course Title:
Microbiology
Course Number:
Biology 2014; (4) 3L, 4LL
Credit Hours:
Four
Catalog Description: Emphasis in lecture on basic concepts of bacteria, viruses, fungi, protzoans and
immunological principles as they relate to man and his environment. Emphasis in
laboratory on identification and handling of microorganisms. Prerequisite: Any
college level biology and chemistry course
Schedule:
Lecture
Lab
Instructor:
Office Location:
Office Hours:
Phone:
Textbook:
I.
2:00 pm-3:20 pm
12:00 pm-1:50 pm; 3:30 pm-5:20 pm
Sherry Gibbany MA MT(ASCP)
A110
Posted on office door
870-743-3000 (ext. 288) or 870-391-3288 (voice mail)
Microbiology; Tortora 10th Edition
Rationale
It is important that everyone have a working knowledge of microorganisms, since they impact
our everyday life so heavily. Microorganisms are everywhere and some cause diseases, while
others prevent and/or cure disease. They are important in the food, sanitation, agricultural,
pharmaceutical, petroleum, environmental and medical professions.
One must have a working knowledge of the fundamental principles of microbiology and
immunology to successfully pursue careers in Allied Health fields, such as Nursing, Medical
Technology, Emergency Medical Technology or Occupational Therapy. Degrees in Medicine,
Biology, Agriculture, Sanitation - Waste Water Treatment, Genetics, etc. usually require one or
more courses in microbiology.
II.
Course Objectives
Upon successful completion of this course students will be able to:
A. define microbiology, list important individuals in the history of microbiology and
differentiate among the groups of microorganisms and their classification.
B. compare and contrast the morphological types of microorganisms and the overall
cell structure/function of prokaryotes and eukaryotes. Identify structures involved
in pathogenicity of prokaryotes.
C. Define genetics, genomes, DNA, RNA, transcription, translation and mutation:
compare and contrast methods of genetic transfer, biotechnology, genetic
engineering and recombinant DNA technology.
D. describe factors that influence microbial growth, phases of a typical microbial
growth curve and methods of measuring growth.
E. list physical, chemical and chemotherapeutic agents that interfere with microbial
growth and identify their use and mode of action.
F. describe viruses, their morphology, structure, composition, cultivation, infectious
process and methods of controlling virus infections. Compare and contrast true
viruses, persistent viral infections, prions and viroids.
G. identify components of the immune system. Compare and contrast non-specific
and specific immunity and the components involved. List representative immune
disorders.
H. identify representative microbial diseases of the upper respiratory tract, lower
respiratory tract, skin, gastrointestinal tract and urinary tract.
I. identify the non-medical applications of microbiology in everyday life.
Assessment of course objectives will use one or more of the following.
1.
2.
3.
4.
5.
III.
Methods of Instruction
1.
2.
3.
4.
5.
6.
7.
IV.
pre and post testing
1 or 2 minute papers
muddiest point
empty outlines
rapid response devices
Lecture will be one mode of instruction.
Questions and classroom discussion(s) will be used in cooperative learning
experiences.
Power Point presentations will be used. Other types of technology will be used when
appropriate. Each lecture will be posted as a lecture capture on portal for individuals
who missed class or need to review the lecture.
Reading assignments from the text and from external sources including the internet
may be required. Summaries of pertintent articles are usually required.
Learning will be enhanced by "hands on" laboratory experiments selected to reinforce
concepts presented in the lectures.
Unknowns will be given in the laboratory requiring the student to apply existing
knowledge, to seek additional information if required and to make decisions on their
own.
Laboratory experiments will be recorded and turned in for grading. This is designed to
reinforce the need to record and report data carefully.
Course Content
This course will be divided into five major areas of study.
I.
Fundamentals of Microbiology
II.
Survey of the Microbial World
III.
Interaction Between Microbe and Host
IV.
Microorganisms and Human Disease
V.
Applied and Environmental Microbiology
SEE THE ENCLOSED OUTLINE FOR SPECIFIC TOPICS.
V.
Grading
A.
LECTURE:
The number of points of total accumulation will vary depending on the number of
quizzes or assignments I feel necessary to adequate learning of the material.
There will be a minimum of three (3) major exams (100 points) each = 300 points
The final exam will be partially comprehensive and will represent Exam 3.
Additional points will be accumulated in the form of small quizzes, internet
assignments, critical thought questions, and/or article summaries. Participation in the
form of “clicker” questions and answers will be used.
Final grades will be assigned on the basis of a 10 point scale.
90-100 = A
80- 89 = B
70- 79 = C
60- 69 = D
< 60 = F
B.
LABORATORY:
Lab grades will be determined separately and will be integrated into an overall final
grade in the course. Lab grades will count for 25% of the course grade.
Grading:
Four 25 point quizzes
Two sets of unknowns
Research assignments
Lab books
Lab practical
Attendance
100 points
50 points
100 points
75 points
50 points
25 points
400 points
*Lab books must be completed including answers to questions for each exercise and
the data reported. These constitute the lab assignment section.
Experimental Research assignment
VI.
1.
This will be done in pairs so need to choose a partner of your choice at the
beginning of the semester.
2.
The groups will choose a topic to research. This is a hands on experimental
research project.
3.
The chosen topic must be approved by the instructor before the student
proceeds.
4.
Once the topic has been approved then the student must come up with an
experimental procedure and plan of action in writing which must be approved
by the instructor.
5.
All lab supplies, media, test kits, etc. will be supplied by the instructor.
6.
Once the lab experiment is successfully completed as approved by the
instructor the experiment must be written in scientific format and turned in.
7.
The students will be graded on participation, effort put into the project,
completeness and presentation.
Attendance
Attendance requirements will be in accordance with school policy. Missing six class hours
may result in the student being dropped with a “W” grade. Missed classes will be solely up to
the student to make up. Missed exams will require the student to obtain permission of the
instructor for make-up. Ms. Polly Jenkins will administer an approved make-up in the testing
labs. A student missing 6 class hours in a 4 credit our course may be dropped from the class.
The roll or seating chart will be checked at the start of each class. If you are late to class, it is
your responsibility to make sure you have not been marked absent. Repeated tardiness will
not be tolerated. You are responsible for all missed materials, assignments, etc.
LAB ATTENDANCE AND COMPLETION
Lab is a required part of the course, failure to attend lab can result in a drop of one letter grade
earned. Missing six labs will result in a drop of one letter grade. I.e., if you have earned a
letter of a “B” after all points are totaled and you have mixed six or more hours in lab your
letter grade will drop to a “C”. No makeup of missed lab quizzes or assignments. NO
EXCEPTIONS.
VII.
Suggestion for success:
A.
Tutoring:
Individual tutors are available through Mr. Robert Markle in the counselor’s offices. All
tutors are free to students.
B.
Computer assisted instruction:
A number of web sites contain excellent microbiology articles. The instructor will
provide these for you periodically or feel free to ask for suggestions. Many are
available on blackboard as part of the microbiology class material
The microbiology place website referred to in the text contains review questions,
animations and interactive materials.
VIII.
C.
Instructor:
I am always available to you when not in class or a committee meeting. Office hours
are posted on my door and I am more likely to be available during these times. Feel
free to drop by at any time other than regular office hours. My office telephone number
is on the front of this syllabus.
D.
Other Students
Find a “study buddy” or “study group”. Your understanding of the material is likely to
increase by discussing it with and/or explaining it to others.
E.
Other helpful hints:
1.
Review class notes while fresh on your mind – between class meetings. 10-15
minutes spent each time will dramatically reduce the need to “cram” before an
exam.
2.
Read the text before the material is covered in class. Then use the text to
clarify unclear lecture notes and for reading topics of interest. Do not get
bogged down in material not included in class.
3.
Seek help when needed.
F.
Quia
This is a tutorial site that I have subscribed to that is great for fun learning games, I
have posted practice quizzes and exams. At the beginning of the semester I will
get email addresses from each of you so that I can get you in the system.
The website is quia.com. The Shared Activities is available at any time.
G.
You tube
I will post numerous helpful you tube sites on portal under course material.
Academic Dishonesty:
Plagiarism of published material, copying other students assignments including lab
books/reports and cheating on exams are all forms of academic dishonesty. They represent
academic dishonesty and will result in immediate dismissal form the course with a grade of 0.
IX.
Safety:
Laboratory involves handling “live” microorganisms. Good aseptic technique must be used at
all times. Students must become familiar with lab rules described in the preface of lab manual
and those described by the lab instructor. Inoculating loops must be sterilized before and after
transfer of each culture. Work area must be disinfected before and after each exercise. In
case of spill, cover the area with disinfectant and immediately notify the instructor. No food or
drink is allowed in the laboratory. Hands must be washed when entering and leaving the lab.
Do not pour live cultures down the sinks.
X.
Student Responsibilities
Read the college catalog and all materials you receive during registration. These materials
tell you what the college expects of you.
Read the syllabus for each class. The syllabus tells you what the instructor expects from
you.
Attend all class meetings. Something important to learning happens during every class
period. If you must miss a class meeting, talk to the instructor in advance about what you
should do.
Be on time. If you come in after class has started, you disrupt the entire class.
Never interrupt another class to talk to the instructor or a student in that class.
Be prepared for class. Complete reading assignments and other homework before class
so that you can understand the lecture and participate in discussion. Always have
pen/pencil, paper, and other specific tools for class.
Learn to take good notes. Write down ideas rather than word-for-word statements by the
instructor.
Allow time to use all the resources available to you at the college. Visit your instructor
during office hours for help with material or assignments you do not understand; use the
library; use the free tutors, tapes, computers, and other resources in the Learning
Assistance Center.
Treat other with respect. Part of the college experience is being exposed to people with
ideas, values, and backgrounds different from yours. Listen to others and evaluate ideas
on their own merit.
XI.
Accommodations for students with special needs:
If you feel you may require accommodations based on the impact of a disability, please
meet with the instructor privately to discuss needs. In addition, please contact a disabilities
coordinator to establish eligibility and coordinate reasonable accommodations. For more
information, contact Kim Brecklein, Room M188, 391-3338 (voice) or
kbrecklein@northark.edu.
COURSE OUTLINE
TEXT:
Microbiology, by Tortora, Funk and Case; 10th Edition
I.
Introduction: Scope and History of Microbiology, Chapters 1, 10
A.
What is Microbiology and what does in include?
B.
Microbial Activities - beneficial and detrimental – Chapters 27 & 28
C.
Classification: Where do microorganisms fit in the overall scheme of things?
II.
Morphology, Anatomy and Fine structure of Prokaryotes, Chapter 4;
Microbial Mechanisms of Pathogenicity, Chapter 15
A.
Morphological characteristics of prokaryotes
B.
Anatomy of the procaryotic cell
C.
Structure and function of the prokaryotic cell
D.
Structure of eukaryotes
EXAM #1
III.
Microbial Genetics, Chapters 8 & 9
A.
Composition and structure of the bacterial chromosome
B.
DNA, RNA and protein synthesis
C.
Mutations
D.
Processes of gene transfer among microorganisms
E.
Genetic engineering: principles, benefits, and hazards
IV.
Viruses, Chapter 13
A.
Definition
B.
Types of viruses
C.
Morphology and composition
D.
Cultivation and infectious process
E.
Slow viruses, viroids and prions
F.
Antiviral agents
G.
Transduction
H.
Latent viruses, oncogenes and cancer
V.
Growth of Microorganisms, Chapter 6
A.
Physical factors that influence growth
B.
Chemical factors that influence growth
C.
Nutritional requirements, growth media and culture methods
D.
Growth curve
VI.
Control of Microbial Growth, Chapters 7 & 20
A.
Definitions and terms
B.
Physical agents
C.
Chemical agents
D.
Chemotherapeutic agents
VII.
Immunology, Chapters 16, 17, 18 & 19
A.
Natural immunity
B.
Acquired immunity
C.
Immune disorders
EXAM #2
EXAM #3
VIII.
Pathogenic Microbiology, chapters 15, 21, 22, 23, 24, 25 & 26
A.
Microbial diseases of the skin and eyes
B.
Diseases of the nervous system
C.
Microbial diseases of the cardiovascular and lymphatic system
D.
Microbial diseases of respiratory system
E.
Microbial diseases of the digestive system
If time allows
Wednesday, May 2, 2:00 – 4:00 pm – Final Exam
NO CLASSES February 22 or 24, 2012 (CLEC conference Salt Lake City), however, there will be
webcams that will need to be viewed for lecture and each student will be expected to have viewed the
webcams, expect a potential quiz on Feb 29 when I return.
March 19 – 23 – NO CLASSES – Spring Break
MICROBIOLOGY 2014
LAB SCHEDULE
Lab Manual:
Benson’s Microbiological Applications, by Alfred Brown, 12th edition
Experiment
Number
Date
1,2,3
Jan 11
I.
Orientation to the Microbiology Laboratory
Introduction to the lab: Ground rules and microscopy
(pp.ix-xvi, 1-22 & 33-42). Chapter 3 of text.
II.
Microscopic Study and Morphology of Microorganisms
10, 11, 14
Jan 13
Handling Cultures, Smear Preparations, Simple Stain & Gram Stain (pp.
85-92; 99-102). Chapter 3 of text.
12, 13,
Jan 18
Negative Stain and Capsule Stain (pp. 91-92; 93-94; lab
discussion)
15, 16
Jan 20
Endospore Stain and Acid Fast Stain (pp. 103-105; 107-108; lab
discussion)
34
Jan 25
Morphological unknown (pp. 231-234); written LAB QUIZ #1
III.
Cultivation of Microorganisms and Viruses
8, 9, 18
Jan 27
Culture Media and Pure Culture Techniques (pp. 121-128;61-67;&71-78)
Chapter 6 of text.
6, 19
Feb 1
Pour Plates, Population Counts and Culturing
Microorganisms from the Environment (pp. 131-138; 47-48)
-------
Feb 3
Read results and discuss and do gram stains on environmental
culture.
21
Feb 8
Bacteriophages (pp 151-154)
Test kit
-------
Feb 10
Read and discuss results
IV.
Environmental Influences and Control of Microorganisms
24, 25, 26
28, 48
Feb 15
Temperature: Effects on growth and lethal effects (pp. 177-178; 183
184). Effects of Oxygen on Growth. (pp 169-173) Chapter 7 of text.
Ultraviolet Light: Lethal Effects (pp 191-192) Temperature (pp 329-31)
------
Feb 17
Review and Discuss Results
30, 31, 32
33
Feb 29
Evaluation of Disinfectants and Antimicrobic susceptibility testing (pp.
201-217). Effectiveness of Handwashing (pp221-224) Chapters 7 and 20
of text.
Experiment
Number
Date
-----
March 2
Discussion of Lab Results – LAB QUIZ #2
V.
Diagnostic Microbiology in Action
35, 36, 38
March 7
Cultural and Physiological Charactertics (pp. 237-240; 241-251)
Physiological Characteristics (pp. 259-262)
37
March 9
Hydrolysis Reactions (pp. 253-257)
Discussion of March 7 results
39
March 14
Discussion of Results and LAB QUIZ #3
Summary of physiological characteristics (pp 267-272)
Primary media for isolation of microorganisms and Lab Presentation.
Appendix C.
40, 41, 42
March 16
Identification of Gram negative Enteric bacteria (non-pathogens) (pp. 371378, pp 275-286, pp 291-295)
53
March 28
Identification of Enteric Pathogens (pp. 371-375) and Lab Presentation.
51, 52
56
March 30
The Staphylococci; the Streptococci and Lab Presentation (pp 347-352;
pp. 357-366, pp 393 -395)
------
April 4
Read results and discuss
LAB QUIZ #4
Work on flow charts for unknowns
------
April 6
NO LAB – catch up day
------
April 11
Unknowns #1
------
April 13
Unknown #1
------
April 18
Continue unknown #1 if needed and start Unknown #2
------
April 20
Continue unknown #2
------
April 25 & 27
Continue unknowns or work on research project
handout
Scheduled labs may be altered dependent on availability of supplies, etc., or due to the laboratory needs. At
least one molecular genetics experiment will be added to the above schedule during one of the Lab discussion
periods, open periods, or at another pre-announced time, if time permits.
Lab time may be used for lecture should lecture time become critical and more lecture time needed.
NO LABS NOVEMBER 23 - THANKSGIVING
SYLLABUS
ACKNOWLEDGEMENT
Course:
Microbiology
Semester:
Spring 2012
I acknowledge by signing below that I have received the syllabus of the
course indicated above. I have reviewed the syllabus and understand the
objectives of this course. Further, I understand how my performance will be
evaluated and how my final grade will be determined. I am aware of my
instructor’s office hours, and I know how to contact him or her for help with
and/or clarification of course contents or procedures.
__________________________________________
Please print name legibly
__________________________________________
Student signature
__________________________________
Date