Official Course Syllabus
Microbiology – BIOL 2014
Fall 2015
Credit Hours:
Four
Schedule:
WF
Instructor:
Dr. Michael Ukpong
Department:
Life Sciences
Office:
Room S206G (hours posted on door)
Phone:
870-391-3562
E-Mail:
mukpong@northark.edu
Lecture 2:00 – 3:20 pm
Lab 12: 00- 1:50 pm & 3:30 – 5:20 pm
General Information
Course Title:
Microbiology
Course Number:
Biology 2014; (4) 3L, 4LL
Credit Hours:
Four
Catalog Description: Emphasis in lecture on basic concepts of bacteria, viruses, fungi, protzoans and
immunological principles as they relate to man and his environment. Emphasis in
laboratory on identification and handling of microorganisms. Prerequisite: Any
college level biology and chemistry course
Schedule:
Lecture
Lab
Textbook:
Lecture: Microbiology; Nester 11th Edition, Lab: Microbiological applications; brown and
Smith 13th (short version)
I.
2:00 pm-3:20 pm
12:00 pm-1:50 pm; 3:30 pm-5:20 pm
Rationale
It is important that everyone have a working knowledge of microorganisms, since they impact
our everyday life heavily. Microorganisms are everywhere and some cause diseases, while
others prevent and/or cure disease. They are important in the food, sanitation, agricultural,
pharmaceutical, petroleum, environmental and medical professions.
One must have a working knowledge of the fundamental principles of microbiology and
immunology to successfully pursue careers in Allied Health fields, such as Nursing, Medical
Technology, Emergency Medical Technology or Occupational Therapy. Degrees in Medicine,
Biology, Agriculture, Sanitation - Waste Water Treatment, Genetics, etc. usually require one or
more courses in microbiology.
II.
Course Objectives
Upon successful completion of this course students will be able to:
A. define microbiology, list important individuals in the history of microbiology and
differentiate among the groups of microorganisms and their classification.
B. compare and contrast the morphological types of microorganisms and the overall
cell structure/function of prokaryotes and eukaryotes. Identify structures involved
in pathogenicity of prokaryotes.
C. Define genetics, genomes, DNA, RNA, transcription, translation and mutation:
compare and contrast methods of genetic transfer, biotechnology, genetic
engineering and recombinant DNA technology.
D. describe factors that influence microbial growth, phases of a typical microbial
growth curve and methods of measuring growth.
E. list physical, chemical and chemotherapeutic agents that interfere with microbial
growth and identify their use and mode of action.
F. describe viruses, their morphology, structure, composition, cultivation, infectious
process and methods of controlling virus infections. Compare and contrast true
viruses, persistent viral infections, prions and viroids.
G. identify components of the immune system. Compare and contrast non-specific and
specific immunity and the components involved. List representative immune disorders.
H. identify representative microbial diseases of the upper respiratory tract, lower respiratory
tract, skin, gastrointestinal tract and urinary tract.
I. identify the non-medical applications of microbiology in everyday life.
J. Learn to think
Assessment of course objectives will use one or more of the following.
1.
2.
3.
4.
5.
III.
Methods of Instruction
1.
2.
3.
4.
5.
6.
7.
IV.
pre and post testing
1 or 2 minute papers
muddiest point
empty outlines
rapid response devices
Lecture will be one mode of instruction.
Questions and classroom discussion(s) will be used in cooperative learning
experiences. Participation is expected.
Power Point presentations will be used. Other types of technology will be used when
appropriate. Each lecture will be posted as a lecture capture on portal for individuals
who missed class or need to review the lecture.
Reading assignments from the text and from external sources including the internet may be
required. Summaries of pertintent articles are usually required.
Learning will be enhanced by "hands on" laboratory experiments selected to reinforce concepts
presented in the lectures.
Unknowns will be given in the laboratory requiring the student to apply existing knowledge, to
seek additional information if required and to make decisions on their own.
Laboratory experiments will be recorded and turned in for grading. This is designed to reinforce
the need to record and report data carefully.
Course Content
This course will be divided into five major areas of study.
I.
Fundamentals of Microbiology
II.
Survey of the Microbial World
III.
Interaction between Microbe and Host
IV.
Microorganisms and Human Disease
V.
Applied and Environmental Microbiology
SEE THE ENCLOSED OUTLINE FOR SPECIFIC TOPICS.
V.
Grading
A.
LECTURE:
The number of points of total accumulation will vary depending on the number of quizzes or
assignments I feel necessary to adequate learning of the material.
There will be a minimum of four (4) major exams (100 points) each = 400 points
I reserved the right to make the final exam comprehensive.
There will be one major project worth at least 100 points.
Additional points maybe accumulated in the form of small quizzes, internet assignments, critical
thought questions, case studies and/or article summaries.
Final grades will be assigned on the basis of a 10 point scale.
90-100 = A
80- 89 = B
70- 79 = C
60- 69 = D
< 60 = F
B.
LABORATORY:
Lab grades will be determined separately and will be integrated into an overall final grade in the
course. Lab grades will count for 25% of the course grade.
Grading:
quizzes/skills test
Two sets of unknowns
Lab books
Lab practical
Attendance
150 points
50 points
25 points
50 points
25 points
300 points
*Lab books must be completed including answers to questions for each exercise and the data
reported. These constitute the lab assignment section.
VI.
Attendance
Attendance requirements will be in accordance with school policy. It is up to the student to drop if lack
of attendance or desire to not complete the course. It is not up to the instructor! The instructor will
make an attempt through email or phone call to contact any student who has missed an excessive
amount of classes or labs. Missed classes or labs will be solely up to the student to make up. Missed
exams will require the student to obtain permission of the instructor for make-up. The testing center will
administer an approved make-up in the testing labs. The roll or seating chart will be checked at the
start of each class. If you are late to class, it is your responsibility to make sure you have not been
marked absent. Repeated tardiness will not be tolerated. You are responsible for all missed materials,
assignments, etc.
If a quiz is given at the beginning of class and you are tardy you will not be allowed to makeup
the inclass quiz.
If absent from class (lecture) period NO makeup of missed lecture quizzes or in class assignments will
be allowed for any circumstances. Refer to the policy of lab makeups in the section of the syllabus that
is titles LAB ATTENDANCE AND COMPLETION.
If I make an exception for one person then I have to make exceptions to other cases so this is blanket
statement for all students. THERE WILL BE NO EXCEPTIONS.
However, missed exams will be allowed to be made up in the testing center but the student will not
have the same opportunities as if the exam was taken in during class time. Also only one make up
exam will be allowed.
LAB ATTENDANCE AND COMPLETION
Lab is a required part of the course, failure to attend lab can result in a drop of one letter grade earned.
Missing six labs will result in a drop of one letter grade. I.e., if you have earned a letter of a “B” after all
points are totaled and you have mixed six or more hours in lab your letter grade will drop to a “C”. No
makeup of missed lab quizzes or assignments. NO EXCEPTIONS.
VII.
Suggestion for success:
A.
Tutoring:
Individual tutors are available through Mr. Robert Markle in the counselor’s offices. All tutors
are free to students.
B.
Computer assisted instruction:
A number of web sites contain excellent microbiology articles. The instructor will provide these
for you periodically or feel free to ask for suggestions.
The microbiology place website referred to in the text contains review questions, animations and
interactive materials.
C.
Instructor:
I am always available to you when not in class or a committee meeting. Office hours are posted
on my door and I am more likely to be available during these times. Feel free to drop by at any
time other than regular office hours. My office telephone number is on the front of this syllabus.
D.
Other Students
Find a “study buddy” or “study group”. Your understanding of the material is likely to increase
by discussing it with and/or explaining it to others.
E.
Other helpful hints:
1.
Review class notes while fresh on your mind – between class meetings. 10-15 minutes
spent each time will dramatically reduce the need to “cram” before an exam.
2.
Read the text before the material is covered in class. Then use the text to clarify unclear
lecture notes and for reading topics of interest. Do not get bogged down in material not
included in class.
3.
Seek help when needed.
VIII.
Academic Dishonesty:
Plagiarism of published material, copying other students assignments including lab books/reports and
cheating on exams are all forms of academic dishonesty. They represent academic dishonesty and will
result in immediate dismissal form the course with a grade of 0.
IX.
Safety:
Laboratory involves handling “live” microorganisms. Good aseptic technique must be used at all times.
Students must become familiar with lab rules described in the preface of lab manual and those
described by the lab instructor. Inoculating loops must be sterilized before and after transfer of each
culture. Work area must be disinfected before and after each exercise. In case of spill, cover the area
with disinfectant and immediately notify the instructor. No food or drink is allowed in the laboratory.
Hands must be washed when entering and leaving the lab. Do not pour live cultures down the sinks.
X.
Student Responsibilities
Read the college catalog and all materials you receive during registration. These materials tell you
what the college expects of you.
Read the syllabus for each class. The syllabus tells you what the instructor expects from you.
Attend all class meetings. Something important to learning happens during every class period. If
you must miss a class meeting, talk to the instructor in advance about what you should do.
Be on time. If you come in after class has started, you disrupt the entire class.
Never interrupt another class to talk to the instructor or a student in that class.
Be prepared for class. Complete reading assignments and other homework before class so that
you can understand the lecture and participate in discussion. Always have pen/pencil, paper, and
other specific tools for class.
Learn to take good notes. Write down ideas rather than word-for-word statements by the instructor.
Allow time to use all the resources available to you at the college. Visit your instructor during office
hours for help with material or assignments you do not understand; use the library; use the free
tutors, tapes, computers, and other resources in the Learning Assistance Center.
Treat other with respect. Part of the college experience is being exposed to people with ideas,
values, and backgrounds different from yours. Listen to others and evaluate ideas on their own
merit.
XI.
Accommodations for students with special needs:
If you feel you may require accommodations based on the impact of a disability, please meet with
the instructor privately to discuss needs. In addition, please contact a disabilities coordinator to
establish eligibility and coordinate reasonable accommodations. For more information, contact Kim
Brecklein, Room M188, 391-3338 (voice) or kbrecklein@northark.edu.
XII.
Weather Make Up Days:
If an excessive amount of class is missed due to inclement weather it is possible that
class lectures may be posted on portal with use of lecture capture for students to view.
It will be the student’s responsibility to view lectures. When deemed necessary online exams
may be given due to excessive missed time in the classroom.
COURSE OUTLINE
TEXT:
Microbiology, by Nester, Anderson, and Roberts; 7th Edition
I.
Introduction: Scope and History of Microbiology, Chapters 1, 10
A.
What is Microbiology and what does in include?
B.
Microbial Activities - beneficial and detrimental – Chapters 29, 30 and 31
C.
Classification: Where do microorganisms fit in the overall scheme of things?
D.
Introduction to central dogma
E.
Biomolecules Chapter 2
F.
Three domains classification system
II.
Morphology, Anatomy and Fine structure of Prokaryotes, Chapter 3;
Microbial Mechanisms of Pathogenicity, Chapter 21-27 & 16
A.
Morphological characteristics of prokaryotes
B.
Anatomy of the prokaryotic cell
C.
Structure and function of the prokaryotic cell
D.
Structure of eukaryotes
III.
Microbial Genetics and Respiration, Chapters 6, 7, 8 & 9
A.
Composition and structure of the bacterial chromosome
B.
DNA, RNA and protein synthesis (central dogma)
C.
Mutations
D.
Processes of gene transfer among microorganisms
E.
Genetic engineering: principles, benefits, and hazards
F.
Respiration: aerobic, anaerobic and fermentation
EXAM #1
IV.
Growth of Microorganisms, Chapter 4, 5 & 6
A.
Physical factors that influence growth
B.
Chemical factors that influence growth
C.
Nutritional requirements, growth media and culture methods
D.
Growth curve
V.
Control of Microbial Growth, Chapters 20
A.
Definitions and terms
B.
Physical agents
C.
Chemical agents
D.
Chemotherapeutic agents
EXAM #2
EXAM #3
VI.
Viruses, Chapter 13
A.
Definition
B.
Types of viruses
C.
Morphology and composition
D.
Cultivation and infectious process
E.
Slow viruses, viroids and prions
F.
Antiviral agents
G.
Transduction
H.
Latent viruses, oncogenes and cancer
VII.
Immunology, Chapters 14, 15, 17 & 18
A.
Natural immunity
B.
Acquired immunity
C.
Immune disorders
D.
Other Diseases
EXAM #4
MICROBIOLOGY BIOL2014
LAB SCHEDULE
Lab Manual:
Benson’s Microbiological Applications, by Alfred Brown, 12th edition
Experiment
Number
Date
I.
1,2,3
Orientation to the Microbiology Laboratory
Aug 19
Introduction to the lab: Ground rules and microscopy. Handling Cultures.
II.
Microscopic Study and Morphology of Microorganisms
10, 11, 14
Aug 21
Smear Preparations, Simple Stain & Gram Stain.
12, 13,
Aug 26
Negative Stain and Capsule Stain
15, 16
Aug 28
Endospore Stain and Acid Fast Stain
34
Sep 2
written LAB QUIZ #1
III.
Cultivation of Microorganisms and Viruses
8, 9, 18
Sep 4
Culture Media and Pure Culture Techniques. Epidemiology experiment.
6, 19
Sep 9
Pour Plates, Population Counts and Culturing
Microorganisms from the Environment.
-------
Sep 11
Read results and discuss and do gram stains on environmental
culture.
IV.
Environmental Influences and Control of Microorganisms
24, 25, 26
28, 48
Sep 16
Temperature: Effects on growth and lethal effects.
Effects of Oxygen on Growth.
Ultraviolet Light: Lethal Effects. Temperature.
------
Sep 18
Continue experiments. Review and Discuss Results
30, 31, 32
33
Sep 23
Evaluation of Disinfectants and Antimicrobic susceptibility testing.
Effectiveness of Handwashing.
Experiment
Number
Date
-----
Discussion of Lab Results – LAB QUIZ #2
Sep 25
V.
Diagnostic Microbiology in Action
35, 36, 38
Sep 30
Cultural and Physiological Charactertics.
Physiological Characteristics.
37
Oct 1
Hydrolysis Reactions Discussion of results
39
Oct 7
Discussion of Results and LAB QUIZ #3
40, 41, 42
Oct 9
Identification of Gram negative Enteric bacteria (non-pathogens) and lab
presentations.
53
Oct 14
Identification of Enteric Pathogens and Lab Presentation.
51, 52
56
Oct 16
The Staphylococci; the Streptococci and Lab Presentation
------
Oct 21
Read results and discuss
LAB QUIZ #4
Work on flow charts for unknowns
------
Oct 23
Unknowns #1
------
Oct 28
Unknowns #1
------
Oct 30
Unknown #2
------
Nov 4
Unknown #2
------
Nov 6
Continue unknown #2
------
Nov 11
Lab Practicum
------
Nov 13
Molecular Biology
------
Nov 18
Molecular Biology
Scheduled labs may be altered dependent on availability of supplies, etc., or due to the laboratory needs. At
least one molecular genetics experiment will be added to the above schedule during one of the Lab discussion
periods, open periods, or at another pre-announced time, if time permits.
Lab time may be used for lecture should lecture time become critical and more lecture time needed.
Nov. 23-27 (M-F)
Dec. 7-11 (M-F)
Thanksgiving Break, no classes, offices closed
Last week of classes/finals
SYLLABUS ACKNOWLEDGEMENT
Course:
Microbiology
Semester:
Spring 2015
I acknowledge by signing below that I have received the syllabus of the
course indicated above. I have reviewed the syllabus and understand the
objectives of this course. Further, I understand how my performance will be
evaluated and how my final grade will be determined. I am aware of my
instructor’s office hours, and I know how to contact him or her for help with
and/or clarification of course contents or procedures.
__________________________________________
Please print name legibly
__________________________________________
Student signature
__________________________________
Date
What do you want to be when you grow up, graduate/ what do you do now?
Do you have any disabilities, prior commitments, or other issues that could interfere with your ability
to attend class or perform in this class?