Name
XBIO: Chapter 13 - Gene Technology Notes
Date
Period
13-1 DNA Technology
 DNA Technology can be used to:
1. _________________________________________
2. _________________________________________
3. _________________________________________
4. _________________________________________
A. DNA Identification
 _______________________ – the complete genetic material in an individual
 Only about _______ of the human genome varies from person to person
 ____________________ – regions of DNA that does not code for any protein (~98% of
our DNA)
 Differences in lengths of noncoding DNA and other variations account for many of the
differences in DNA among individuals
Steps in DNA Identification
1. _______________________
 ___________________________(PCR) - used to
make multiple copies of DNA
2. ______________________
 Using restriction enzymes
 Restriction enzymes –
____________________________________
____________________________________
3. ______________________________
 Gel Electrophoresis – _______________________________________________
_________________________________________________________________
 Shorter fragments move ____________ and ____________
 _______________ charged DNA moves towards the ______________ side of the
gel electrophoresis tray
 Gel is stained and can be transferred to a film or paper for analysis
4. ___________________________
 DNA Fingerprint – ______________________________________________
_____________________________________________________________
_____________________________________________________________
_____________________________________________________________
 Comparing certain places in the human genome where large variations are
known to occur limits the odds that two people will share a DNA profile to
approximately 1-100 billion
 Who committed the crime???
B. Recombinant DNA
 Genetic engineering - _________________________________________________
___________________________________________________________________
 Recombinant DNA – __________________________________________________
___________________________________________________________________
 Examples
o Jellyfish gene that codes for a green fluorescent protein (GFP) that will glow
under ultraviolet light is inserted into zebrafish so that their blood vessels
will glow
o Gene for pest resistance inserted into plants
o Gene used to alter bacteria for cleaning up toxic waste
o Protein used to dissolve blood clots in heart attack therapy
o Protein used to make snow form at higher temperature
 _______________________________________
Gene of Interest -
Plasmid –