University of Jordan
Faculty of Agriculture
Dept. Horticulture & Crop Science
Semester:
Course name: Plant Tissue Culture (0641732)
Credits: 3
Instructors: Dr. Ayed Al Abdallat
Office: Seed technology building, Office No. 1
Office hours:
Phone: 535 5000 Ext. 22354
Course Description:
This course is designed for graduate students wanting to learn about plant tissue
culture. The course is taught in two 75-minute lectures per week with two exams, midterm
and final. Grading is based on the exams, oral presentation and laboratory work.
Plant tissue culture is the science of growing plant cells, tissues or organs isolated from the
mother plant, on artificial media. It includes techniques and methods used to research into
many botanical disciplines and has several practical objectives.
There is an excellent book specifically about plant tissue culture that has been published
recently:
Plant Propagation by Tissue Culture (2008). Online Resource:
http://www.springerlink.com/content/n5tm30/?p=b93c5ea7cf094758ba340e329c6dfa91&pi=1
This is a useful reference book you might want to have.
Other Useful References:
Plant Cell and Tissue Culture –A Tool in Biotechnology (2009). Online Resource:
http://www.springerlink.com/content/x57035/?p=b93c5ea7cf094758ba340e329c6dfa91&pi=2
Plant Tissue Culture Engineering: (2006). Online Resource:
http://www.springerlink.com/content/p3p448/?p=b93c5ea7cf094758ba340e329c6dfa91&pi=4
Plant Cell and Tissue Culture (1990). Online Resource:
http://www.springerlink.com/content/w88507/?p=b93c5ea7cf094758ba340e329c6dfa91&pi=3
Course Objectives:

This course seeks to familiarize students to the basic principles of plant tissue
culture and to expose them to its applications.

To provide hands-on experience of the most common of these techniques in labs and
demonstrations of more advanced or uncommon techniques.
Expected outcomes:

At the end of the course, you should be able to: disinfest and place into culture
suitable explants capable of being cultured and multiplied, make culture medium
from reagent grade chemicals and stock solutions, routinely transfer cultures
without contamination, and analyze the usefulness of information available from the
scientific literature that deals with plant tissue culture.
Tests and Evaluations:
1. Exams:
a. Final exam:
2. Oral presentation:
3. Lab Activities:
25%
40%
35%
Course Contents
Topics
Lectures
Introduction and course description
Internet Resources for Plant Tissue Culture
Plant Tissue Culture Procedure - Background
Micropropagation: Uses and Methods
Plant Tissue Culture Systems
Plant Tissue Culture Laboratory
Plant Tissue Culture Media
Plant Growth and Development
Plant Growth Regulators
Plant Genetic Variation
Agrobacterium-Mediated Transformation
Presentations
Laboratory Work
1
1
1
1
1
1
1
1
1
1
1
12
6