COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY Lecture page 1 of 9 COURSE SYLLABUS TERM: Summer YEAR: 2012 INSTRUCTOR: Dr. Marta D. de Jesus OFFICE/DIVISION LOCATION: SCI-337 OFFICE HOURS: by appointment OFFICE PHONE: (805) 654-6400 x1275 E-MAIL ADDRESS: mdejesus@vcccd.edu (leave notes with the Division Assistants in SCI-321) INSTRUCTOR WEB PAGE: http://dewhozitz.net/ CLASS HOURS: Lecture: Tu/We/Th 3:00 - 5:50 PM (SCI-313) CLASS CRN: 50284 Lab: Tu/We/Th 11:30 - 2:20 PM (SCI-311) with Prof. E. Bellenson CLASS CRN: 52249 Lab: Tu/We/Th 6:30 - 9:20 PM (SCI-311) with Prof. E. Bellenson PREREQUISITE(S): CHEM V01A-V01AL or CHEM V20A-V20AL or Chem V30-V30L with grades of C or better AND PHYSO V01 or BIOL V04 with grades of C or better. RECOMMENDED PREP: BIOL V01 OR 1 year of HS college prep or honors biology with a passing grade of C or better (note: this is a pre-req for ANAT V01). To see the pre-nursing requirements for VC’s RN Program go to: http://www.venturacollege.edu/departments/academic/nursing_science/applying_to_nursing.shtml and download the Nursing Application. A. COURSE DESCRIPTION This course includes lectures and laboratory studies concerning bacteria, fungi, protists, algae and viruses with emphasis on bacterial morphology, anatomy, staining, classification, metabolism and growth, and the effects of physical and chemical agents on bacteria. The course includes the principles of disease transmission, prevention and biotechnology. It serves several different populations including pre-nursing, biotechnology, and as preparation for graduate professional degrees in physician assistant, physical therapy, pharmacy, etc. Disclaimer: This course is NOT the equivalent of a required class for microbiology majors or medical students (MD or DO), although it may be a useful class in aiding preparation and understanding for those students. Summer Note: This is an accelerated version of MICR V01 as compared to our normal semester. Each day the class meets is the equivalent of 1 week of that class (thus 1 week of summer = 3 weeks of regular term). Recommendation: Do NOT plan on having much time for other activities during the course, do NOT plan any absences. COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY page 2 of 9 B. METHOD OF INSTRUCTION In the “lecture” portion of this class, traditional lecture will be the primary mode of instruction. The lab portion of this class consists mostly of a “hands-on” set of lab exercises (see Schedule). Please see the Schedule and Grading Plan for a list of reading and other assignments, tests, etc. C. STUDENT LEARNING OBJECTIVES 1. Student knows and can apply microbiology and immunology terms and concepts to microbiological problems. 2. Student understands and can compare and contrast taxonomy, biological significance, genetics, and metabolism of microorganisms 3. Follow laboratory protocols and perform microbiologic lab skills, including microscopy, staining, and culturing of microorganisms. D. COURSE OBJECTIVES Upon completion of the lecture portion of this course, the student should be able to: 1. understand basic biological principles particularly as they apply to microorganisms. 2. be familiar with, and able to use the terminology common to microbiology. 3. know the importance of microorganisms in nature. 4. have a general knowledge of microorganisms as agents of disease. 5. be aware of important physical and chemical methods for control of microorganisms. 6. have a general knowledge of applied microbiology. 7. understand the principles, techniques and significance of genetic engineering. 8. understand the mechanisms involved in disease caused by microorganisms. 9. understand the mechanisms of host resistance to pathogenic microorganisms particularly the immune system. 10. understand the basic principles of taxonomy 11. become familiar with the microbiological literature and develop writing skills. 12. be able to demonstrate critical thinking and problem solving using the scientific method. E. COURSE TOPICS/UNITS (see schedule) F. CORE COMPETENCIES Read, retain, and apply published ideas Write clearly and accurately in a variety of contexts and formats Employ the vocabulary of the subject being studied Recognize the need for information and/or identify and clarify the question that needs to be answered Differentiate between major and minor arguments or ideas Find and interpret relevant information from text, tables, graphs, personal communication, and observation Evaluate authority, veracity and bias of information Utilize data gathered to draw conclusions or to create new sources of information that can be shared with others Apply the principles of scientific reasoning to solve problems Use language as appropriate to the situation G. TEXTBOOK(S) AND REQUIRED TOOLS OR SUPPLIES Required Text: Microbiology: with Diseases by Taxonomy (3rd ed.) by Robert Baumann. Bring your textbook to every class meeting. Read the assigned chapters before class! COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY page 3 of 9 Lecture Outlines: To be announced Required Lab Text: Ventura College’s Custom Lab Manual based on Microbiology by Cappucino and Sherman Lab Safety Supplies: labcoat (NOT scrubs!), safety eyewear (glasses or goggles), disposable gloves (your lab instructor may require more items) Optional/Recommended Materials: 1) Student Study Guide (for text) 2) A Photographic Atlas for Microbiology Laboratory (color photos) 3) Dictionary of Word Roots & Combining Forms by D.J. Borror (useful for medical terminology) 4) An Introduction to Chemistry for Biology Students by G. Sackheim (good re/overview of chemistry) 5) Clinical Microbiology Made Ridiculously Simple by M. Gladwin & B. Trattler 6) The Cartoon Guide to Genetics by L. Gonick & M. Wheeler (good introduction to microbial genetics) 7) Coloring books: Microbiology Coloring Book by Alcamo Diversity of Life by Margulis, Schwartz, & Dolan H. GRADING PLAN Guaranteed Grades: A = 90-100% B = 80-89% C = 70-79% D = 60-69% F = below 60% Grading: In general, there are NO MAKE-UPS and late assignments will not be accepted. Grades will be assigned based on work performed. 75% of your grade will come from lecture and 25% will be from lab (actual “points” in the lecture & lab will NOT be of equal weight). (your lecture grade)(.75) + (your lab grade)(.25) = your final numerical grade Eg: (lecture)(.75) (70%)(.75) (66%)(.75) (64%)(.75) (62%)(.75) (60%)(.75) + + + + + + (lab).25 (70%)(.25) (82%)(.25) (88%)(.25) (94%)(.25) (100%)(.25) = 70% I. COURSE COMPONENT SPECIFICS Lecture Tests (50% of your grade): 15-point tests will be given every day in lecture first thing and are over either when the last person to arrive on-time finishes, or a reasonable time has passed. Bring the small Scantron 15-point answer sheets Scantron Form 815-E (Quizstrip). You will also need #2 pencils (other numbers of pencil lead are not read correctly by the machine), and a good eraser (the white plastic ones work best). Question formats can include multiple choice, matching and short answer (write the answer on the back). Each test will cover the material since the last test. The lowest 2 test scores (not including the Final Exam) will be dropped from your final grade tally. No make-ups. Lecture Final Exam (25% of your grade) is cumulative and will contain between 110-120 points; bring one larger Scantron Form #886-E for multiple choice, matching and short answer questions. Lab Scores (25% of your grade): are determined by the lab instructor. Attendance/Participation: Even though there are no direct points given for this, this can affect your ultimate grade. If you are tardy or leave early you will lose points on tests, and you may miss valuable information/announcements. Expect to be present in class the entire time listed (lecture & labs). COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY page 4 of 9 You are responsible for your credit and enrollment status; be aware of Ventura College’s add/drop dates and policies. Note: VCCCD policy states: “When absence from the class exceeds in number 1/9th of the total class contact hours for the session, the instructor may recommend to the Registrar that such student be dropped from the class and a grade recorded in accordance with withdrawal from class policy as stated in (the) Catalog. Classes missed because of late registration are counted toward the number of absences.” Last Day to Withdraw: July 17, 2012 Extra Credit: there is little to none available (both lecture & lab). J. CLASSROOM RULES OF CONDUCT (also Appendix 1 of the Ventura College Catalog: Student Conduct Code or go to http://www.venturacollege.edu/honesty/). 1) Academic Dishonesty (a.k.a. Cheating): This can take a variety of forms. In Academia in general, at Ventura College, and especially in this class, it will NOT be tolerated. All work you submit for grading (assignments, papers, & exams) must be your own work. In this course you are being graded as an individual and your work must reflect that. Dishonest behavior includes: a) looking at another’s test/exam/quiz; b) allowing someone else see your test/exam/quiz; c) passing information in any form during an test/exam/quiz (talking will NOT be tolerated); d) using crib notes or other “aids” during a test/exam/quiz; e) plagiarism = using someone else’s words as your own. This includes copying your assignment from another student or allowing someone else to make a copy of your assignment, and paraphrasing which means using the same words/phrases as the original author even if they are rearranged. At the least, the first time academic dishonesty becomes an issue, you may be moved and you will receive a ZERO GRADE for the assignment. If the incident is outrageous or a second offense, sterner measures will be taken. A Word to the Wise: Since you have chosen to continue in academia and into a profession with very high ethical standards, realize that behavior that may have been treated as a minor infraction earlier in school will have increasingly serious consequences if it occurs now or later in your academic or professional career. 2) Disruption/Obstruction of class: None will be tolerated. Beepers, telephones, etc. must be turned audibly OFF during classroom time, and any disruptive behavior (this includes talking out of turn or answering a text message) will get you excluded from class. Please leave the class if you cannot comport yourself as required. During tests & exams all communication devices will be off the desks (= not visible) and you cannot respond to calls at that time. *If you have an emergency situation, please discuss it the instructor prior to the exam or lecture, or as soon as possible. 3) Lab Safety: As this class has a laboratory component, there is a required introduction to lab safety. No student will be allowed to participate in lab until this instruction has taken place. K. Some of the On-Campus Student Help: see the online Summer 2012 schedule of classes for a complete list. Please make use of these services if needed! 1) Tutoring Center: offers free tutoring in most academic subjects including biology, chemistry, math, etc. as well as help with general study skills. {Incidentally, if you are doing well in his or any other class & are willing to help others (& get paid for it), PLEASE consider signing up as a tutor!} Note: Qualified tutors are very difficult to find for this class, often there are NONE available like this summer so far. COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY page 5 of 9 2) Counseling & Career Centers: Counselors can help to define your educational goals & the path to achieve them. For nursing (LVN, & RN), talk to Bea Herrera or Angelica Gonzalez. For 4-year (i.e., transfer) majors (including BSN programs) there are other counselors (Marcelino De Cierdo is in charge). To do your own research for how VC’s programs and classes match up with your target institution(s), California public colleges & universities only, go to: www.assist.org. (Note: Please realize that I and your other professors can answer some questions about transfer & careers. We’ve been to some of the schools & know about careers in our majors.) 3) Special academic needs: Educational Assistance Center, ATTC for Students with Disabilities: “If you have a documented disability that may require accommodations in this class, please see the EAC (then me) as soon as possible so that your learning needs can be appropriately met.” If you’d like to be tested for a learning disability please contact the EAC as soon as possible. 4) Extended Opportunity Programs & Services: has a variety of ways to help economically disadvantaged students. 5) The Student Health Center can treat many problems. There are nominal fees for laboratory tests, procedures and medications. L. EMERGENCY PROCEDURES FOR ACCIDENTS AND ACUTE ILLNESS Campus Emergency Numbers From any pay phone on campus From off-campus phones Campus Police Business Office College Nurse Dial 911 from on-campus #9 642-7000 x6486 x6346 9-911 a) In life-threatening situations, dial 9-911. Give the nature and exact location of the accident or medical emergency! DO NOT MOVE THE VICTIM, unless to prevent further injury! b) For non-life-threatening situations, contact the Student Health Center (x6346). A nurse is on duty during daytime hours and limited evening hours. When the nurse is not available or the Student Health Center is closed, call 642-7000 (first Aid is available from Campus Police when the Student Health Center is closed). Please report ALL student college-related injuries, as soon as possible to the Student Health Center. The instructor or person in charge shall make a written report of the injury. Accident report forms are available at the Student Health Center, the Evening Dean's office, or from Campus Police. Accident reports are essential to process Insurance Claims; both are processed through the Student Health Center. Once an accident report is received that requires an Insurance Claim, the student will be given information about the college's insurance coverage and the billing procedures. An injury resulting from an illness is NOT covered by the college insurance, but it still needs to be reported to the Student Health Center! Ventura College insurance coverage for college-related injuries is limited, and it does not guarantee payment in full! M. Other miscellaneous helps: Online useful study skill & test-taking advice (there are lots of sites; here are just a few & some cross-reference each other) http://ollie.dcccd.edu/Services/StudyHelp/StudySkills/ http://www.uni.edu/walsh/linda7.html http://www.ucc.vt.edu/stdysk/stdyhlp.html http://www.howtostudy.org/ http://www.studygs.net/ COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY Nearby Medical Libraries (these are open to the public) page 6 of 9 1) St. Johns Regional Medical Library (SJRMC) 1600 N. Rose Ave. Oxnard, CA 93030 Phone 805.988.2820 www.stjohnslibrary.org Monday - Friday 8:30 a.m. - 5:00 p.m. Medical reference books & journals (including many on microbiology). It does have Internet access (I don’t know their access policy), and librarians will do research for a fee (& if they have the time). 2) UCLA Biomedical Library (see hours & policies on the UCLA website: www.ucla.edu) Some Online Microbiology resources (there are lots more & they vary in technical level): Powers of 10 http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/ Molecular Expressions: Exploring the World of Optics & Microscopy http://micro.magnet.fsu.edu/index.html Highlights in the History of Microbiology http://users.stlcc.edu/kkiser/History.page.html MicrobeWorld http://www.microbeworld.org/ The Grapes of Staph http://student.ccbcmd.edu/~gkaiser/goshp.html MicrobeWiki http://microbewiki.kenyon.edu/index.php/MicrobeWiki MicrobiologyBytes http://www.microbiologybytes.com/blog/ UC Museum of Paleontology: Collections http://www.ucmp.berkeley.edu/collections/other.html Pond Life Digital Video Gallery http://micro.magnet.fsu.edu/optics/olympusmicd/galleries/moviegallery/pondscum.html National Institute of Allergy & Infectious Disease (part of NIH) http://www.niaid.nih.gov/Pages/default.aspx CDC http://www.cdc.gov/ FDA http://www.fda.gov/default.htm Bad Bug Book http://www.fda.gov/Food/FoodSafety/FoodborneIllness/FoodborneIllnessFoodbornePathogens NaturalToxins/BadBugBook/default.htm (easier- just type in “Bad Bug Book” into a search engine) ALGAL-ED http://silicasecchidisk.conncoll.edu/Algal-ED_finished.html Tom Volk’s Fungi http://botit.botany.wisc.edu/toms_fungi/ Dr. Fungus http://www.doctorfungus.org Parasites On Parade http://www.cbu.edu/~seisen/ParasitesOnParade.htm Animal And Human Parasite Images http://www.k-state.edu/parasitology/625tutorials/index.html Medical Entomology http://extension.entm.purdue.edu/publichealth/resources.html All the Virology on the WWW http://www.virology.net/garryfavweb.html ViralZone http://expasy.org/viralzone/ The Original Medical Wiki http://wikidoc.org/index.php/Main_Page Online texts (some are medical school microbiology books; none replace your textbook): The Microbial World http://www.microbiologytext.com/index.php Todar's Online Textbook of Bacteriology http://www.textbookofbacteriology.net/ Microbiology And Immunology Online http://pathmicro.med.sc.edu/book/welcome.htm 2 major professional societies: The American Society of Microbiology Society for General Microbiology http://www.asm.org/ with http://www.microbemagazine.org/ http://www.sgm.ac.uk/ {Note: the non-textbook images I use usually come from the Internet; use the Images/Photos option when you search to find them or other similar photos.} DISCLAIMER: This syllabus is subject to change and may do so depending on circumstances. Every attempt will be made to advise the class of these changes in a timely manner and make the changes equitable. Any grading changes/adjustments will be made in an equitable manner based on work performed. Being absent from class and missing any announcements does not constitute an excuse for missing announcements or assignments. Types of Questions (Bloom’s Taxonomy; Bloom et al. 1956) used at the college level: (page 7 of 9) You (as a college student) should be able to answer questions that test you on course material that are in ANY of these formats (not just the first one, knowledge): Knowledge remembering memorizing recognizing recalling identification recalling information Examples: who, what, when, where, how ___ ? describe __ Comprehension interpreting translating from one medium to another describing in one's own words organization and selection of facts and ideas Example: retell __ *Application problem solving applying information to produce some result use of facts, rules and principles Examples: how is __ an example of ...? how is __ related to ...? why is __ significant? *Analysis subdividing something to show how it is put together finding the underlying structure of a communication identifying motives separation of a whole into component parts Examples: what are the parts or features of __ ? classify __ according to __ outline/diagram __ how does __ compare/contrast with __ ? what evidence can you list for __ ? Synthesis creating a unique, original product that may be in verbal form or may be a physical object combination of ideas to form a new whole Examples: what would you predict/infer from __ ? what ideas can you add to __ ? how would you create/design a new __ ? what might happen if you combined __ ? what solutions would you suggest for __ ? Evaluation making value decisions about issues resolving controversies or differences of opinion development of opinions, judgements or decisions Examples: do you agree that __ ? (then explain why!) what do you think about __ ? (then explain why!) what is the most important __ ? (then explain why!) place the following in order of priority __ (then explain why!) how would you decide about __ ? (then explain why!) what criteria would you use to assess __ ? (then explain why!) COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY N. WEEKLY OR DAILY PLANNED SCHEDULE - Su12 (tentative; subject to change) Week 1 Tu 6/19 W 6/20 Lecture Introduction to Microbiology & History Microscopy Microscopy Cell Structure & Function Text Chapters Ch 1 Ch 4 Ch 4 Ch 3 (Last Day to Add or Drop with Refund) Th 6/21 Cell Structure & Function Microbial Metabolism Ch 3 Ch 5 page 8 of 9 Laboratory Exercises Lab Intro & Safety Ubiquity of Bacteria I 5, 11 Aseptic Technique I Ubiquity of Bacteria II 5, 11 Aseptic Technique II 33, 35 Microscopy Kingdom Survey - Part A (195, 197 Protists) 53 Smear 59 Simple Stain Kingdom Survey - Part B 69 Gram Stain 81 Endospore Stain (Last Day to Drop w/o W) Week 2 Tu 6/26 W 6/27 Th 6/28 Week 3 Tu 7/3 Lecture Microbial Metabolism Microbial Nutrition & Growth Microbial Nutrition & Growth Controlling Microbial Growth - Environment Text Chapters Ch 5 Ch 6 Laboratory Exercises 17 Pure Culture I 75 Acid-fast Stain Ch 6 Controlling Microbial Growth – Environment Characterizing & Classifying Prokaryotes Ch 9 17 Pure Culture II 189, 279 Unknown Bacterium I Stains of Unknown (Gram, Endospore, Acid-fast) 27 Culture Characteristics 109 Temperature Effects I 189, 279 Unknown Bacterium II 27 Culture Characteristics II 109 Temperature Effects II 143, 151 Fermentation I 157 TSI Agar I 161 IMViC I 177 Nitrate Reduction I 181 Catalase I 185 Oxidase I Lecture Characterizing & Classifying Prokaryotes Text Chapters Ch 11 (& 19, 20, 21) Ch 9 Ch 11 (& 19, 20, 21) Laboratory Exercises 143, 151 Fermentation II 157 TSI Agar II 161 IMViC II 177 Nitrate Reduction II 181 Catalase II 185 Oxidase II 143, 145 Hydrolysis I 173 Urease I Blood Agar I MAC & Mannitol Salt Agar I COURSE NUMBER: MICR V01 COURSE TITLE: GENERAL MICROBIOLOGY N. WEEKLY OR DAILY PLANNED SCHEDULE - Su12 (tentative) page 9 of 9 Week 3 W 7/4 Th 7/5 Lecture Holiday Characterizing & Classifying Prokaryotes Text Chapters Laboratory Exercises Ch 11 (& 19, 20, 21) 143, 145 Hydrolysis II 173 Urease II Blood Agar II MAC & Mannitol Salt Agar II 115, 121 Anaerobe Jar, FTM I Bergey's Manual Week 4 Tu 7/10 Lecture Characterizing & Classifying Eukaryotes Text Chapters Ch 12 (& 22, 23) W 7/11 Characterizing & Classifying Eukaryotes Ch 12 (& 22, 23) Th 7/12 Microbial Genetics Ch 7 Laboratory Exercises 115, 121 Anaerobe Jar, FTM II Alcohol as an Antiseptic I 235 Disinfectants I 225 Kirby Bauer Test I Alcohol as an Antiseptic II 235 Disinfectants II 225 Kirby Bauer Test II Enumeration of bacteria I Hand Scrubbing I Lab Practical Exam Enumeration of Bacteria II Hand Scrubbing II Week 5 Tu 7/17 Lecture Recombinant DNA Technology (short) Text Chapters Ch 8 (Last Day to Drop w W) W 7/18 Th 7/19 Week 6 Tu 7/24 Characterizing & Classifying Viruses, Viroids & Prions Characterizing & Classifying Viruses, Viroids & Prions Immunity Lecture Infection & Epidemiology Antimicrobial Drugs (short) W 7/25 **Final Exam** + not in lab manual Laboratory Exercises +Bacterial Transformation I +ELISA Unknown Due Ch 13, (& 24, 25) +Bacterial Transformation II +Plaque Assay I Ch 13, (& 24, 25) +Plaque Assay II Review Ch 15, Ch 16 Text Chapters Ch 14 Laboratory Exercises Final Lab Practical Exam Ch 10 (none) Note: for labs labeled I & II: I is when the cultures are subjected to those procedures and II is the day the results are observed and recorded. (Blank page) COURSE NUMBER: MICR V01 Summer 2012 CRN: 50284 52249 (Please circle which of the above CRNs apply to you) COURSE TITLE: GENERAL MICROBIOLOGY AFFIDAVIT My signature below indicates that I have read and understood this syllabus and have been given a copy of my own to keep. Print Name: Student Signature Need this back by next class period! Date Student Information Sheet Class: MICR V01 Please circle which of these CRNs apply to you: Name: (last) Semester: Summer 2012 Section: 50284 52249 Nickname? (first) Student ID No. Telephone No. Preferred email address: Major? How long have you been in college? (note: “pre-???” is NOT usually a major) Have you finished a college degree? Planning to transfer? What kind? Where? Eventual employment goals? What college Life Sciences classes have you had or are in now? What college Chemistry classes have you had or are in now? What college Math, Engineering or other science classes have you had or are in now? What is the reason you are taking this class? Is there anything else you want to tell me as you start the class?