COURSE NUMBER - M. de Jesus

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COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY Lecture
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COURSE SYLLABUS
TERM: Summer
YEAR: 2012
INSTRUCTOR: Dr. Marta D. de Jesus
OFFICE/DIVISION LOCATION: SCI-337
OFFICE HOURS: by appointment
OFFICE PHONE: (805) 654-6400 x1275
E-MAIL ADDRESS: mdejesus@vcccd.edu
(leave notes with the Division Assistants in SCI-321)
INSTRUCTOR WEB PAGE: http://dewhozitz.net/
CLASS HOURS: Lecture: Tu/We/Th 3:00 - 5:50 PM (SCI-313)
CLASS CRN: 50284
Lab: Tu/We/Th 11:30 - 2:20 PM (SCI-311) with Prof. E. Bellenson
CLASS CRN: 52249
Lab: Tu/We/Th 6:30 - 9:20 PM (SCI-311) with Prof. E. Bellenson
PREREQUISITE(S): CHEM V01A-V01AL or CHEM V20A-V20AL or Chem V30-V30L with
grades of C or better AND PHYSO V01 or BIOL V04 with grades of C or better.
RECOMMENDED PREP: BIOL V01 OR 1 year of HS college prep or honors biology with a
passing grade of C or better (note: this is a pre-req for ANAT V01).
To see the pre-nursing requirements for VC’s RN Program go to:
http://www.venturacollege.edu/departments/academic/nursing_science/applying_to_nursing.shtml
and download the Nursing Application.
A. COURSE DESCRIPTION
This course includes lectures and laboratory studies concerning bacteria, fungi, protists, algae
and viruses with emphasis on bacterial morphology, anatomy, staining, classification, metabolism and
growth, and the effects of physical and chemical agents on bacteria. The course includes the principles
of disease transmission, prevention and biotechnology. It serves several different populations including
pre-nursing, biotechnology, and as preparation for graduate professional degrees in physician
assistant, physical therapy, pharmacy, etc.
Disclaimer: This course is NOT the equivalent of a required class for microbiology majors or medical
students (MD or DO), although it may be a useful class in aiding preparation and understanding for
those students.
Summer Note: This is an accelerated version of MICR V01 as compared to
our normal semester. Each day the class meets is the equivalent of 1 week of
that class (thus 1 week of summer = 3 weeks of regular term).
Recommendation: Do NOT plan on having much time for other activities
during the course, do NOT plan any absences.
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
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B. METHOD OF INSTRUCTION
In the “lecture” portion of this class, traditional lecture will be the primary mode of instruction.
The lab portion of this class consists mostly of a “hands-on” set of lab exercises (see Schedule).
Please see the Schedule and Grading Plan for a list of reading and other assignments, tests, etc.
C. STUDENT LEARNING OBJECTIVES
1. Student knows and can apply microbiology and immunology terms and concepts to microbiological
problems.
2. Student understands and can compare and contrast taxonomy, biological significance, genetics, and
metabolism of microorganisms
3. Follow laboratory protocols and perform microbiologic lab skills, including microscopy, staining, and
culturing of microorganisms.
D. COURSE OBJECTIVES
Upon completion of the lecture portion of this course, the student should be able to:
1. understand basic biological principles particularly as they apply to microorganisms.
2. be familiar with, and able to use the terminology common to microbiology.
3. know the importance of microorganisms in nature.
4. have a general knowledge of microorganisms as agents of disease.
5. be aware of important physical and chemical methods for control of microorganisms.
6. have a general knowledge of applied microbiology.
7. understand the principles, techniques and significance of genetic engineering.
8. understand the mechanisms involved in disease caused by microorganisms.
9. understand the mechanisms of host resistance to pathogenic microorganisms particularly the
immune system.
10. understand the basic principles of taxonomy
11. become familiar with the microbiological literature and develop writing skills.
12. be able to demonstrate critical thinking and problem solving using the scientific method.
E. COURSE TOPICS/UNITS (see schedule)
F. CORE COMPETENCIES

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Read, retain, and apply published ideas
Write clearly and accurately in a variety of contexts and formats
Employ the vocabulary of the subject being studied
Recognize the need for information and/or identify and clarify the question that needs to be
answered
Differentiate between major and minor arguments or ideas
Find and interpret relevant information from text, tables, graphs, personal communication, and
observation
Evaluate authority, veracity and bias of information
Utilize data gathered to draw conclusions or to create new sources of information that can be
shared with others
Apply the principles of scientific reasoning to solve problems
Use language as appropriate to the situation
G. TEXTBOOK(S) AND REQUIRED TOOLS OR SUPPLIES
Required Text: Microbiology: with Diseases by Taxonomy (3rd ed.) by Robert Baumann.
Bring your textbook to every class meeting. Read the assigned chapters before class!
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
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Lecture Outlines: To be announced
Required Lab Text: Ventura College’s Custom Lab Manual based on Microbiology by
Cappucino and Sherman
Lab Safety Supplies: labcoat (NOT scrubs!), safety eyewear (glasses or goggles), disposable
gloves (your lab instructor may require more items)
Optional/Recommended Materials:
1) Student Study Guide (for text)
2) A Photographic Atlas for Microbiology Laboratory (color photos)
3) Dictionary of Word Roots & Combining Forms by D.J. Borror (useful for medical terminology)
4) An Introduction to Chemistry for Biology Students by G. Sackheim (good re/overview of chemistry)
5) Clinical Microbiology Made Ridiculously Simple by M. Gladwin & B. Trattler
6) The Cartoon Guide to Genetics by L. Gonick & M. Wheeler (good introduction to microbial genetics)
7) Coloring books:
Microbiology Coloring Book by Alcamo
Diversity of Life by Margulis, Schwartz, & Dolan
H. GRADING PLAN
Guaranteed Grades:
A = 90-100%
B = 80-89%
C = 70-79%
D = 60-69%
F = below 60%
Grading: In general, there are NO MAKE-UPS and late assignments will not be accepted.
Grades will be assigned based on work performed. 75% of your grade will come from lecture
and 25% will be from lab (actual “points” in the lecture & lab will NOT be of equal weight).
(your lecture grade)(.75) + (your lab grade)(.25) = your final numerical grade
Eg:
(lecture)(.75)
(70%)(.75)
(66%)(.75)
(64%)(.75)
(62%)(.75)
(60%)(.75)
+
+
+
+
+
+
(lab).25
(70%)(.25)
(82%)(.25)
(88%)(.25)
(94%)(.25)
(100%)(.25)
= 70%
I. COURSE COMPONENT SPECIFICS
Lecture Tests (50% of your grade): 15-point tests will be given every day in lecture first thing and are
over either when the last person to arrive on-time finishes, or a reasonable time has passed. Bring the
small Scantron 15-point answer sheets Scantron Form 815-E (Quizstrip). You will also need #2 pencils
(other numbers of pencil lead are not read correctly by the machine), and a good eraser (the white
plastic ones work best). Question formats can include multiple choice, matching and short answer
(write the answer on the back). Each test will cover the material since the last test. The lowest 2 test
scores (not including the Final Exam) will be dropped from your final grade tally. No make-ups.
Lecture Final Exam (25% of your grade) is cumulative and will contain between 110-120 points;
bring one larger Scantron Form #886-E for multiple choice, matching and short answer questions.
Lab Scores (25% of your grade): are determined by the lab instructor.
Attendance/Participation: Even though there are no direct points given for this, this can affect
your ultimate grade. If you are tardy or leave early you will lose points on tests, and you may
miss valuable information/announcements. Expect to be present in class the entire time listed
(lecture & labs).
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
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You are responsible for your credit and enrollment status; be aware of Ventura College’s
add/drop dates and policies. Note: VCCCD policy states: “When absence from the class
exceeds in number 1/9th of the total class contact hours for the session, the instructor may
recommend to the Registrar that such student be dropped from the class and a grade recorded
in accordance with withdrawal from class policy as stated in (the) Catalog. Classes missed
because of late registration are counted toward the number of absences.”
Last Day to Withdraw: July 17, 2012
Extra Credit: there is little to none available (both lecture & lab).
J. CLASSROOM RULES OF CONDUCT (also Appendix 1 of the Ventura College Catalog:
Student Conduct Code or go to http://www.venturacollege.edu/honesty/).
1) Academic Dishonesty (a.k.a. Cheating): This can take a variety of forms. In Academia in
general, at Ventura College, and especially in this class, it will NOT be tolerated. All work you
submit for grading (assignments, papers, & exams) must be your own work. In this course you
are being graded as an individual and your work must reflect that. Dishonest behavior
includes:
a) looking at another’s test/exam/quiz;
b) allowing someone else see your test/exam/quiz;
c) passing information in any form during an test/exam/quiz (talking will NOT be tolerated);
d) using crib notes or other “aids” during a test/exam/quiz;
e) plagiarism = using someone else’s words as your own. This includes copying your
assignment from another student or allowing someone else to make a copy of your
assignment, and paraphrasing which means using the same words/phrases as the original
author even if they are rearranged.
At the least, the first time academic dishonesty becomes an issue, you may be moved
and you will receive a ZERO GRADE for the assignment. If the incident is outrageous or a
second offense, sterner measures will be taken.
A Word to the Wise: Since you have chosen to continue in academia and into a profession with
very high ethical standards, realize that behavior that may have been treated as a minor
infraction earlier in school will have increasingly serious consequences if it occurs now or later
in your academic or professional career.
2) Disruption/Obstruction of class: None will be tolerated. Beepers, telephones, etc. must
be turned audibly OFF during classroom time, and any disruptive behavior (this includes
talking out of turn or answering a text message) will get you excluded from class. Please
leave the class if you cannot comport yourself as required. During tests & exams all
communication devices will be off the desks (= not visible) and you cannot respond to calls at
that time.
*If you have an emergency situation, please discuss it the instructor prior to the exam or
lecture, or as soon as possible.
3) Lab Safety: As this class has a laboratory component, there is a required introduction to
lab safety. No student will be allowed to participate in lab until this instruction has taken place.
K. Some of the On-Campus Student Help: see the online Summer 2012 schedule of
classes for a complete list. Please make use of these services if needed!
1) Tutoring Center: offers free tutoring in most academic subjects including biology, chemistry, math,
etc. as well as help with general study skills. {Incidentally, if you are doing well in his or any other class
& are willing to help others (& get paid for it), PLEASE consider signing up as a tutor!} Note: Qualified
tutors are very difficult to find for this class, often there are NONE available like this summer so far.
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
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2) Counseling & Career Centers: Counselors can help to define your educational goals & the path to
achieve them. For nursing (LVN, & RN), talk to Bea Herrera or Angelica Gonzalez. For 4-year (i.e.,
transfer) majors (including BSN programs) there are other counselors (Marcelino De Cierdo is in
charge). To do your own research for how VC’s programs and classes match up with your target
institution(s), California public colleges & universities only, go to: www.assist.org.
(Note: Please realize that I and your other professors can answer some questions about transfer &
careers. We’ve been to some of the schools & know about careers in our majors.)
3) Special academic needs: Educational Assistance Center, ATTC for Students with Disabilities:
“If you have a documented disability that may require accommodations in this class, please see the
EAC (then me) as soon as possible so that your learning needs can be appropriately met.” If you’d like
to be tested for a learning disability please contact the EAC as soon as possible.
4) Extended Opportunity Programs & Services: has a variety of ways to help economically
disadvantaged students.
5) The Student Health Center can treat many problems. There are nominal fees for laboratory tests,
procedures and medications.
L. EMERGENCY PROCEDURES FOR ACCIDENTS AND ACUTE ILLNESS
Campus Emergency Numbers
From any pay phone on campus
From off-campus phones
Campus Police Business Office
College Nurse
Dial 911 from on-campus
#9
642-7000
x6486
x6346
9-911
a) In life-threatening situations, dial 9-911.
Give the nature and exact location of the accident or medical emergency! DO NOT MOVE THE
VICTIM, unless to prevent further injury!
b) For non-life-threatening situations, contact the Student Health Center (x6346).
A nurse is on duty during daytime hours and limited evening hours. When the nurse is not available or
the Student Health Center is closed, call 642-7000 (first Aid is available from Campus Police when the
Student Health Center is closed).
Please report ALL student college-related injuries, as soon as possible to the Student Health
Center. The instructor or person in charge shall make a written report of the injury. Accident report
forms are available at the Student Health Center, the Evening Dean's office, or from Campus Police.
Accident reports are essential to process Insurance Claims; both are processed through the Student
Health Center. Once an accident report is received that requires an Insurance Claim, the student will be
given information about the college's insurance coverage and the billing procedures.
An injury resulting from an illness is NOT covered by the college insurance, but it still needs to
be reported to the Student Health Center! Ventura College insurance coverage for college-related
injuries is limited, and it does not guarantee payment in full!
M. Other miscellaneous helps:
Online useful study skill & test-taking advice (there are lots of sites; here are just a few & some
cross-reference each other)
http://ollie.dcccd.edu/Services/StudyHelp/StudySkills/
http://www.uni.edu/walsh/linda7.html
http://www.ucc.vt.edu/stdysk/stdyhlp.html
http://www.howtostudy.org/
http://www.studygs.net/
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
Nearby Medical Libraries (these are open to the public)
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1) St. Johns Regional Medical Library (SJRMC)
1600 N. Rose Ave. Oxnard, CA 93030
Phone 805.988.2820 www.stjohnslibrary.org
Monday - Friday 8:30 a.m. - 5:00 p.m. Medical reference books & journals (including many on
microbiology). It does have Internet access (I don’t know their access policy), and librarians will do
research for a fee (& if they have the time).
2) UCLA Biomedical Library (see hours & policies on the UCLA website: www.ucla.edu)
Some Online Microbiology resources (there are lots more & they vary in technical level):
Powers of 10
http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/
Molecular Expressions: Exploring the World of Optics & Microscopy
http://micro.magnet.fsu.edu/index.html
Highlights in the History of Microbiology
http://users.stlcc.edu/kkiser/History.page.html
MicrobeWorld
http://www.microbeworld.org/
The Grapes of Staph http://student.ccbcmd.edu/~gkaiser/goshp.html
MicrobeWiki
http://microbewiki.kenyon.edu/index.php/MicrobeWiki
MicrobiologyBytes
http://www.microbiologybytes.com/blog/
UC Museum of Paleontology: Collections http://www.ucmp.berkeley.edu/collections/other.html
Pond Life Digital Video Gallery
http://micro.magnet.fsu.edu/optics/olympusmicd/galleries/moviegallery/pondscum.html
National Institute of Allergy & Infectious Disease (part of NIH)
http://www.niaid.nih.gov/Pages/default.aspx
CDC http://www.cdc.gov/
FDA http://www.fda.gov/default.htm
Bad Bug Book
http://www.fda.gov/Food/FoodSafety/FoodborneIllness/FoodborneIllnessFoodbornePathogens
NaturalToxins/BadBugBook/default.htm (easier- just type in “Bad Bug Book” into a search engine)
ALGAL-ED
http://silicasecchidisk.conncoll.edu/Algal-ED_finished.html
Tom Volk’s Fungi
http://botit.botany.wisc.edu/toms_fungi/
Dr. Fungus
http://www.doctorfungus.org
Parasites On Parade http://www.cbu.edu/~seisen/ParasitesOnParade.htm
Animal And Human Parasite Images http://www.k-state.edu/parasitology/625tutorials/index.html
Medical Entomology http://extension.entm.purdue.edu/publichealth/resources.html
All the Virology on the WWW http://www.virology.net/garryfavweb.html
ViralZone
http://expasy.org/viralzone/
The Original Medical Wiki
http://wikidoc.org/index.php/Main_Page
Online texts (some are medical school microbiology books; none replace your textbook):
The Microbial World
http://www.microbiologytext.com/index.php
Todar's Online Textbook of Bacteriology
http://www.textbookofbacteriology.net/
Microbiology And Immunology Online
http://pathmicro.med.sc.edu/book/welcome.htm
2 major professional societies:
The American Society of Microbiology
Society for General Microbiology
http://www.asm.org/ with
http://www.microbemagazine.org/
http://www.sgm.ac.uk/
{Note: the non-textbook images I use usually come from the Internet; use the Images/Photos option
when you search to find them or other similar photos.}
DISCLAIMER: This syllabus is subject to change and may do so depending on circumstances. Every
attempt will be made to advise the class of these changes in a timely manner and make the changes
equitable. Any grading changes/adjustments will be made in an equitable manner based on work
performed. Being absent from class and missing any announcements does not constitute an excuse for
missing announcements or assignments.
Types of Questions (Bloom’s Taxonomy; Bloom et al. 1956) used at the college level: (page 7 of 9)
You (as a college student) should be able to answer questions that test you on course material that are in ANY of
these formats (not just the first one, knowledge):
Knowledge
remembering
memorizing
recognizing
recalling identification
recalling information
Examples:
who, what, when, where, how ___ ?
describe __
Comprehension
interpreting
translating from one medium to another
describing in one's own words
organization and selection of facts and ideas
Example:
retell __
*Application
problem solving
applying information to produce some result
use of facts, rules and principles
Examples:
how is __ an example of ...?
how is __ related to ...?
why is __ significant?
*Analysis
subdividing something to show how it is put together
finding the underlying structure of a communication
identifying motives
separation of a whole into component parts
Examples:
what are the parts or features of __ ?
classify __ according to __
outline/diagram __
how does __ compare/contrast with __ ?
what evidence can you list for __ ?
Synthesis
creating a unique, original product that may be in verbal form or may be a physical object
combination of ideas to form a new whole
Examples:
what would you predict/infer from __ ?
what ideas can you add to __ ?
how would you create/design a new __ ?
what might happen if you combined __ ?
what solutions would you suggest for __ ?
Evaluation
making value decisions about issues
resolving controversies or differences of opinion
development of opinions, judgements or decisions
Examples:
do you agree that __ ? (then explain why!)
what do you think about __ ? (then explain why!)
what is the most important __ ? (then explain why!)
place the following in order of priority __ (then explain why!)
how would you decide about __ ? (then explain why!)
what criteria would you use to assess __ ? (then explain why!)
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
N. WEEKLY OR DAILY PLANNED SCHEDULE - Su12 (tentative; subject to change)
Week 1
Tu 6/19
W 6/20
Lecture
Introduction to
Microbiology & History
Microscopy
Microscopy
Cell Structure &
Function
Text Chapters
Ch 1
Ch 4
Ch 4
Ch 3
(Last Day to Add or Drop
with Refund)
Th 6/21
Cell Structure &
Function
Microbial Metabolism
Ch 3
Ch 5
page 8 of 9
Laboratory Exercises
Lab Intro & Safety
Ubiquity of Bacteria I
5, 11 Aseptic Technique I
Ubiquity of Bacteria II
5, 11 Aseptic Technique II
33, 35 Microscopy
Kingdom Survey - Part A (195, 197
Protists)
53 Smear
59 Simple Stain
Kingdom Survey - Part B
69 Gram Stain
81 Endospore Stain
(Last Day to Drop w/o W)
Week 2
Tu 6/26
W 6/27
Th 6/28
Week 3
Tu 7/3
Lecture
Microbial Metabolism
Microbial Nutrition &
Growth
Microbial Nutrition &
Growth
Controlling Microbial
Growth - Environment
Text Chapters
Ch 5
Ch 6
Laboratory Exercises
17 Pure Culture I
75 Acid-fast Stain
Ch 6
Controlling Microbial
Growth – Environment
Characterizing &
Classifying Prokaryotes
Ch 9
17 Pure Culture II
189, 279 Unknown Bacterium I
Stains of Unknown (Gram,
Endospore, Acid-fast)
27 Culture Characteristics
109 Temperature Effects I
189, 279 Unknown Bacterium II
27 Culture Characteristics II
109 Temperature Effects II
143, 151 Fermentation I
157 TSI Agar I
161 IMViC I
177 Nitrate Reduction I
181 Catalase I
185 Oxidase I
Lecture
Characterizing &
Classifying Prokaryotes
Text Chapters
Ch 11 (& 19, 20,
21)
Ch 9
Ch 11 (& 19, 20,
21)
Laboratory Exercises
143, 151 Fermentation II
157 TSI Agar II
161 IMViC II
177 Nitrate Reduction II
181 Catalase II
185 Oxidase II
143, 145 Hydrolysis I
173 Urease I
Blood Agar I
MAC & Mannitol Salt Agar I
COURSE NUMBER: MICR V01
COURSE TITLE: GENERAL MICROBIOLOGY
N. WEEKLY OR DAILY PLANNED SCHEDULE - Su12 (tentative)
page 9 of 9
Week 3
W 7/4
Th 7/5
Lecture
Holiday
Characterizing &
Classifying Prokaryotes
Text Chapters
Laboratory Exercises
Ch 11 (& 19, 20,
21)
143, 145 Hydrolysis II
173 Urease II
Blood Agar II
MAC & Mannitol Salt Agar II
115, 121 Anaerobe Jar, FTM I
Bergey's Manual
Week 4
Tu 7/10
Lecture
Characterizing &
Classifying Eukaryotes
Text Chapters
Ch 12 (& 22, 23)
W 7/11
Characterizing &
Classifying Eukaryotes
Ch 12 (& 22, 23)
Th 7/12
Microbial Genetics
Ch 7
Laboratory Exercises
115, 121 Anaerobe Jar, FTM II
Alcohol as an Antiseptic I
235 Disinfectants I
225 Kirby Bauer Test I
Alcohol as an Antiseptic II
235 Disinfectants II
225 Kirby Bauer Test II
Enumeration of bacteria I
Hand Scrubbing I
Lab Practical Exam
Enumeration of Bacteria II
Hand Scrubbing II
Week 5
Tu 7/17
Lecture
Recombinant DNA
Technology (short)
Text Chapters
Ch 8
(Last Day to Drop w W)
W 7/18
Th 7/19
Week 6
Tu 7/24
Characterizing &
Classifying Viruses,
Viroids & Prions
Characterizing &
Classifying Viruses,
Viroids & Prions
Immunity
Lecture
Infection &
Epidemiology
Antimicrobial Drugs
(short)
W 7/25
**Final Exam**
+ not in lab manual
Laboratory Exercises
+Bacterial Transformation I
+ELISA
Unknown Due
Ch 13, (& 24, 25) +Bacterial Transformation II
+Plaque Assay I
Ch 13, (& 24, 25) +Plaque Assay II
Review
Ch 15, Ch 16
Text Chapters
Ch 14
Laboratory Exercises
Final Lab Practical Exam
Ch 10
(none)
Note: for labs labeled I & II:
I is when the cultures are subjected to those procedures and
II is the day the results are observed and recorded.
(Blank page)
COURSE NUMBER: MICR V01 Summer 2012
CRN: 50284
52249
(Please circle which of the above CRNs apply to you)
COURSE TITLE: GENERAL MICROBIOLOGY
AFFIDAVIT
My signature below indicates that I have read and understood this syllabus and
have been given a copy of my own to keep.
Print Name:
Student Signature
Need this back by next class period!
Date
Student Information Sheet Class: MICR V01
Please circle which of these CRNs apply to you:
Name:
(last)
Semester:
Summer 2012
Section: 50284
52249
Nickname?
(first)
Student ID No.
Telephone No.
Preferred email address:
Major?
How long have you been in college?
(note: “pre-???” is NOT usually a major)
Have you finished a college degree?
Planning to transfer?
What kind?
Where?
Eventual employment goals?
What college Life Sciences classes have you had or are in now?
What college Chemistry classes have you had or are in now?
What college Math, Engineering or other science classes have you had or are in
now?
What is the reason you are taking this class?
Is there anything else you want to tell me as you start the class?
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