MEDICAL MICROBIOLOGY
1. INTRODUCTION
Medical microbiology is an essential component in the infectious disease field and
knowledge in this area is vital to the clinical management of infections. Microbiology
unit is particularly involved in isolation or establishing the causative agents as well as
monitoring and screening of diseases.
2. LIST OF SERVICES
Microbiology unit provides the following services:
2.1 Diagnostic microbiological services which comprise of bacteriology, mycology,
parasitology, serology and immunology.
2.2 Participation in hospital wide infection control activities related to surveillance,
control and prevention of nosocomial infections.
2.3 Provision of microbiologic studies of the hospital environment and sterility testing.
2.4 Microbiological investigations for medicolegal cases.
3. CONTACT NUMBERS
Location/Section
Head of Unit
Dr. Nurzam Suhaila Bt Che
Hussin
Office
Senior Scientific Officer
Pn. Hjh. Zaidatul Azmi Bt
Abdul Rahman
Scientific Officers Room
Bacteriology – Main Laboratory
Parasitology Laboratory
Mycology Laboratory
Serology & Immunology
Laboratory
Senior Medical Lab
Technologist Room
Line B
5590
Line C
6857
5613
5618
5614
5615
5616
6859
6860
5627
Direct line
26155590
LIST AND TESTS DONE IN MICROBIOLOGY LABORATORY
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
REMARK
(Urgent/STAT)
Done by lab staff
1.
Air sampling
Microbiology
Not Applicable
Not Applicable
Not Applicable
By
Appointment
3 days
Main Counter
2.
Allergy Testing (Screening)
Microbiology
Plain Container
Blood
5 ml
Tue, Wed, Fri
3 days
Main Counter
3.
Allergy Testing (Specific)
Microbiology
Plain Container
Blood
5 ml
Tue, Wed, Fri
3 days
Main Counter
4.
Anti double-stranded DNA
Microbiology
Plain Tube
Blood
5 ml
Thu
7 days
Main Counter
5.
Antifungal Susceptibility Test
Microbiology
Not Applicable
Not Applicable
Not Applicable
Daily
5-14 days
Main Counter
(yeast and yeast-like organism
only)
6.
Anti Mycoplasma pneumonia
IgM
Microbiology
Plain Tube
Blood
5 ml
Mon
3 days
Main Counter
7.
Antibiotic sensitivity testing
Microbiology
Not Applicable
Not Applicable
Not Applicable
Daily
2 days
Main Counter
8.
* Antigen test for cerebrospinal
fluid
Microbiology
Sterile Tube
CSF
2 ml
Daily
1 day
Main Counter
9.
Anti-neutrophil cytoplasmic
antigen (ANCA) – c-ANCA
Microbiology
Plain Tube
Blood
5 ml
Wed
7 days
Main Counter
10.
Anti-neutrophil cytoplasmic
antigen (ANCA) – p-ANCA
Microbiology
Plain Tube
Blood
5 ml
Thu
7 days
Main Counter
11.
Antinuclear antibodies
Microbiology
Plain Tube
Blood
5 ml
Mon, Wed, Fri
3 days
Main Counter
12.
Anti-streptolysin ‘O’ Titre
(ASOT)
Microbiology
Plain Tube
Blood
5 ml
Daily
2 days
Main Counter
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
13.
Anti-thyroglobulin antibodies
Microbiology
Plain Tube
Blood
5 ml
Wed
7 days
Main Counter
14.
Anti-Thyroid microsomal
antibody
Microbiology
Plain Tube
Blood
5 ml
Wed
7 days
Main Counter
15.
Aspirate culture & sensitivity
Microbiology
Sterile Container
Aspirate
Not Applicable
Daily
3 days
Main Counter
16. * Blood culture & sensitivity
(aerobic)
Microbiology
Aerobic Blood Culture
Bottle
Blood
5-10 ml
Daily
3-5 days
Main Counter
17. * Blood culture & sensitivity
(anaerobic)
Microbiology
Anaerobic Blood
Culture Bottle
Blood
5-10 ml
Daily
3-5 days
Main Counter
18.
Blood Film Malaria parasites
(BFMP)
Microbiology
Thick/thin film
Blood Smear
Not Applicable
Daily
1 hr
Main Counter
19.
Blood Fungal culture &
sensitivity
Microbiology
Blood Fungal
Container
Blood
5 ml
Daily
5-14 days
Main Counter
20.
Bronchial aspirate culture &
sensitivity
Microbiology
Sterile Container
Broncheal Alveolar
Larvage
Not Applicable
Daily
3 days
Main Counter
21. * Cerebrospinal fluid culture &
sensitivity
Microbiology
Sterile Tube
CSF
3 ml
Daily
2-3 days
Main Counter
22.
Chlamydia trachomatis
Immunofluorescence
Microbiology
Smear
Genital discharge /
Eye discharge
Not Applicable
Daily
3 days
Main Counter
23.
Chlamydia pneumoniae
antibody
Microbiology
Sterile Tube
Blood
5 ml
Wed
2 days
Main Counter
24.
Cryptococcal antigen
Microbiology
Sterile Container
CSF
2 ml
Daily
1 day
Main Counter
25.
Ear/Eye swab culture &
sensitivity
Microbiology
Amies TM
Swab
Not Applicable
Daily
3 days
Main Counter
REMARK
(Urgent/STAT)
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
26.
Efficiency testing on autoclaves
(Attest)
Microbiology
Attest
Attest strip
Not Applicable
Daily
2 days
Main Counter
27.
Extractable Nuclear Antigen Anti Jo1
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
28.
Extractable Nuclear Antigen RNP
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
29.
Extractable Nuclear Antigen
SSA/Ro
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
30.
Extractable Nuclear Antigen-Scl
70
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
31.
Extractable Nuclear AntigenSm
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
32.
Extractable Nuclear AntigenSSB/La
Microbiology
Plain Tube
Blood
5 ml
Wed/Thu
1 day
Main Counter
33.
Fungal culture
Microbiology
Sterile Container
Tissue/hair/nail/skin
Not Applicable
Daily
5-14 days
Main Counter
34.
Genital culture & sensitivity
Microbiology
Amies TM
Swab
Not Applicable
Daily
3 days
Main Counter
35.
Indirect Immunoperoxidase for
Rickettsial
Microbiology
Plain tube
Blood
5 ml
Mon, Wed, Fri
3 days
Main Counter
36.
Legionella pneumophilia
Antigen
Microbiology
Sterile Container
Urine
5 ml
Daily
2 days
Main Counter
37.
Leptospira Antibody IgM
Microbiology
Plain Tube
Blood
5 ml
Thu
7 days
Main Counter
38.
Microfilaria (Blood film)
Microbiology
Thick film
Blood Smear
Not Applicable
Daily
1 day
Main Counter
39.
Modified Ziehl-Neelsen for
Cryptosporidium
Microbiology
Sterile Container
Stool
5 ml
Daily
2 days
Main Counter
REMARK
(Urgent/STAT)
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
REMARK
(Urgent/STAT)
M-F Lytic Blood
Culture Bottlerequest from
Microbiology lab
before collection
40.
Mycobacterium (For
incubation only)
Microbiology
M-F Lytic Blood
Culture Bottle
Blood
5-10 ml
Daily
6 wks
Main Counter
41.
Nasal swabs culture &
sensitivity
Microbiology
Amies TM
Swab
Not Applicable
Daily
3 days
Main Counter
42. * Peritoneal Fluid culture &
sensitivity
Microbiology
Sterile Container
Peritoneal fluid
> 1 ml
Daily
3 days
Main Counter
43.
Pernasal swab for Bordetella
pertussis culture and
sensitivity
Microbiology
Bordet-Gengou Agar
Swab
Not Applicable
Daily
7 days
Main Counter
44.
Pneumocyctis carinii
Immunofluorescence
Microbiology
Sterile Container
Induced Sputum
3 ml
Once a week
3 days
Main Counter
45.
Pus/Swab culture & sensitivity
Microbiology
Amies TM/
Pus/Swab
Not Applicable
Daily
3 days
Main Counter
Sterile Container
46.
Rapid Plasme Reagin (Syphilis)
Microbiology
Plain Tube
Blood
5 ml
Daily
2 days
Main Counter
47.
Rectal Swab for MRO
Microbiology
Sterile Container
Rectal swab
Not Applicable
Screening
Staff/Outbreak
Cases
2-3 days
Main Counter
48.
Respiratory culture &
sensitivity
Microbiology
Sterile Container
Upper and Lower
Respiratory Track
specimen
Not Applicable
Daily
2 days
Main Counter
49.
Rheumatoid Factor
Microbiology
Plain Tube
Blood
5 ml
Tue, Thu
3 days
Main Counter
Bordet-Gengou
Agar- request
from
Microbiology lab
before collection
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
50.
Sterility testing
Microbiology
Sterile Container
Milk, TPN fluid,
Disfectant
5 ml
Daily
7 days
Main Counter
51.
Stool culture & sensitivity
Microbiology
Sterile Container
Stool
3 ml
Daily
2 days
Main Counter
52.
Stool for Clostridium difficile
culture
Microbiology
Sterile Container
Stool
3 ml
Daily
3 days
Main Counter
53.
Stool for Clostridium difficile
toxin
Microbiology
Sterile Container
Stool
3 ml
Daily
7 days
Main Counter
54.
Stool for Isospora
Microbiology
Sterile Container
Stool
3 ml
Daily
2 days
Main Counter
55.
Stool Ova & Cyst
Microbiology
Sterile Container
Stool
3 ml
Daily
2 days
Main Counter
56.
Stool for Rotavirus
Microbiology
Sterile Container
Stool
3 ml
Daily
1 day
Main Counter
57.
Stool Vibrio cholera
Microbiology
APW
Stool
3 ml
Daily
5 days
Main Counter
58.
Streptococcal Pneumoniae
Antigen detection (SPA)
Microbiology
Sterile Container
Urine
5 ml
Daily
1 day
Main Counter
59.
Throat swab culture &
sensitivity
Microbiology
Amies TM
Swab
Not Applicable
Daily
3 days
Main Counter
60.
Tissue culture & sensitivity
Microbiology
Sterile container
Tissue
Not Applicable
Daily
3-5 days
Main Counter
REMARK
(Urgent/STAT)
NO
TEST
UNIT
CONTAINER
SPECIMEN TYPE
VOLUME
SCHEDULE
TAT
(Min/Hr/Day)
COUNTER
61.
Tissue for Helicobacter pylori
culture & sensitivity
Microbiology
Sterile container
Tissue
Not Applicable
Daily
5 days
Main Counter
62.
Treponema Pallidum
Haemagglutination
Microbiology
Plain Tube
Blood
5 ml
Mon, Tue, Fri
3 days
Main Counter
REMARK
(Urgent/STAT)
(TPHA)
63.
Urine culture & sensitivity
Microbiology
Sterile Container
Urine
5 ml
Daily
3 days
Main Counter
64.
Veneral Disease Research
Laboratory Cerebrospinal fluid
(VDRL).
Microbiology
Sterile Container
CSF
3 ml
Daily
7 days
Main Counter
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