BIOTECHNOLOGY
(ADDITIONAL QUESTIONS FOR PRACTICE)
1. Normal and natural function of restriction enzymes in bacteria is
(1) Getting rid of unwanted DNA from phages and other invaders
(2) To join the okazaki fragments formed during replication
(3) To polymerise the nucleotides during duplication of DNA
(4) To join the plasmid DNA with the nucleoid
2. pBR 322 and pUC 18 are plasmids (original / modified ) of
(1) E. coli
(3) Salmonella species
(2) Agrobacterium tumefaciens
(4) Rhizobium
3. ‘Molecular scissors’ and ‘molecular stitchers’ are
(1) Gyrases and ligases respectively
(2) Restriction endonucleases and ligases respectively
(3) Ligases and endonucleases respectively
(4) Primases and ligases respectively
4. RENs cut
(1) ssDNA
(3) ssRNA
(2) dsDNA
(4) dsRNA
5. Select the DNA sequence which would act as a restriction site
(1) 5’ GGTTGG 3’
3’ CCAACC 5’
(3) 5’ AACCGG 3’
3’ TTGGCC 5’
(2) 5’ AAGGCT 3’
3’ TTCCGA 5’
(4) 5’ CTGCAG 3’
3’ GACGTC 5’
6. Some of the components of pUC 18 are
(1) ampr, ori, tetr and MCS
(3) ampr, ori, lac z and MCS
(2) ampr, ori, tetr, lac z and MCS
(4) lac z, ori, tetr and MCS
7. Statement A: During genetically engineered biosynthesis of insulin, A-chain and B-chain are
separated from their respective fusion proteins by treatment with carboxypeptidase
and trypsin.
Statement B: After the separation of A and B chains from fusion proteins, the two chains are
bonded by disulphide bonds by treating them with sodium disulphonate.
(1) Both the statements A and B are correct
(3) Statement A is correct and B is incorrect
(2) Both the statements A and B are incorrect
(4) Statement B is correct and A is incorrect
8. The Bt toxin gene has been introduced into several crop plants. It is
(1) Originally obtained from a bacterium
(3) An artificially synthesized gene
(2) Originally obtained from a bacteriophage
(4) cDNA obtained from a mRNA
9. Which among the following is an ideal sample for extracting DNA for DNA fingerprinting technique
to solve parental disputes ?
(1) Serum
(3) Leucocyte
(2) Erythrocyte
(4) Platelet
A. Sudheendra Hebbar, Sri Ramakrishna Vidyashala, MYSORE – 570020
(ashebbar66@gmail.com)
1
10. VNTRs are
(1) Short segments of DNA that repeat over and over in the non-coding regions of a chromosome
(2) Short segments of DNA that repeat over and over in the coding regions of a chromosome
(3) Short segments of DNA that repeat over and over in both the coding and non-coding regions of a
chromosome
(4) Short segments of DNA that repeat over and over in the exons of a gene
11. Analysis of restriction fragment length polymorphism (RFLP) involves
(1) Electroporation and restriction enzyme digestion
(2) Restriction enzyme digestion and electrophoresis
(3) Electrophoresis only
(4) Treatment of DNA sample with RNA and alkaly only
12. A technique used to make numerous copies of a specific segment of DNA quickly and accurately is
(1) Agarose gel electrophoresis
(3) Eletroporation
(2) Polymerase chain reaction
(4) Transcription
13. Gel electrophoresis is used for
(1) Construction of rDNA by joining with cloning vectors
(2) Isolation of DNA molecules
(3) Cutting of DNA into fragments
(4) Separation of DNA fragments according to their size
14. In gel electrophoresis, the differential mobility of DNA depends on
(1) Helical nature of DNA
(3) Charge and size of DNA
(2) Double stranded nature of DNA
(4) Hydrogen bonding between bases
15. The products of restriction enzyme digestion of a DNA sample can be detected by the technique of
(1) Electroporation
(3) Electrophoresis
(2) Polymerase Chain Reaction
(4) DNA amplification
16. ‘Golden rice’, a GM rice variety is obtained by introducing three alien genes into the genome of
Oryza sativa. The alien genes are from
(1) Erwinia uredovora and daffodil
(3) Agrobacterium tumefaciens and daffodil
(2) Erwinia uredovora and Zea mays
(4) daffodil and Zea mays
17. Tissue culture technique is not as popular in animals as in plants. This is because
(1) Most of the animal cells lack totipotency
(2) It is difficult to maintain infection-free culture
(3) It is difficult to procure cells from animals to culture
(4) Suitable culture medium is not available
18. Which of the following is used for sterilizing culture medium ?
(1) Thermostat
(3) Refrigerator
(2) Incubator
(4) Autoclave
19. The undifferentiated and unorganised mass of tissue in culture is called
(1) Gall
(3) Callose
(2) Callus
(4) Explant
A. Sudheendra Hebbar, Sri Ramakrishna Vidyashala, MYSORE – 570020
(ashebbar66@gmail.com)
2
20. The plant part which is inoculated in tissue culture technique is called
(1) Embryoid
(3) Explant
(2) Callus
(4) Vector
21. In tissue culture technique, caulogenesis refers to
(1) Development of shoot bud
(3) Development of roots
(2) Production of callus
(4) Hardening
22. Hybridoma technique to produce monoclonal antibodies involves the fusion of
(1) B – lymphocyte and T – lymphocyte
(3) T – lymphocyte and myeloma cell
(2) B – lymphocyte and myeloma cell
(4) Spleen cell and B – lymphocyte
23. Prevention of tissue rejection in patients who have undergone kidney transplantation surgery can be
achieved by
(1) rDNA technology
(3) Stem cell culturing and treatment
(2) Monoclonal antibodies
(4) Gene therapy
24. Monoclonal antibodies are important medical tools today due to one of their following properties;
(1) Amazing specificity of matching antigens only
(2) High sensitivity for detection only
(3) Diverse specificity for several antigens
(4) High sensitivity for detection and their specificity for specific antigens
25. Somatic hybridisation involves the fusion of
(1) Two or more nuclei of somatic cells
(2) Cytoplasm of two or more cells
(3) Two or more protoplasts of unrelated vegetative cells
(4) Two gametes
26. The protoplasts of two different plants fuse to form a
(1) Zygote
(3) Embryoid
(2) Embryo
(4) Heterokaryon
27. Somatic cell hybrids are produced with the help of
(1) Polyvenyl glycol
(3) Polypropylene glycol
(2) Polyethylene glycol
(4) Polyhydroxy glycol
28. Embryoids are
(1) Highly active somatic cells of the callus produced during tissue culture
(2) Embryos produced from reproductive cells
(3) Embryos produced from somatic cells in tissue culture
(4) Dead embryos
29. Which of the following chemicals is used in modern biotechnology to double the chromosome number
in any plant ?
(1) Indole acetic acid
(3) Zeatin
(2) Gibberellic acid
(4) Colchicine
30. Stem cells have the property of
(1) Multiplication and regeneration
(3) Multiplication and differentiation
(2) Regeneration and differentiation
(4) Multiplication only
A. Sudheendra Hebbar, Sri Ramakrishna Vidyashala, MYSORE – 570020
(ashebbar66@gmail.com)
3
ANSWER KEY
Q.No.
Ans.
1
1
2
1
3
2
4
2
5
4
6
3
7
4
8
1
9
3
10
1
Q.No.
Ans.
11
2
12
2
13
4
14
3
15
3
16
1
17
1
18
4
19
2
20
3
Q.No.
Ans.
21
1
22
2
23
2
24
4
25
3
26
4
27
2
28
3
29
4
30
3
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A. Sudheendra Hebbar, Sri Ramakrishna Vidyashala, MYSORE – 570020
(ashebbar66@gmail.com)
4