AP Biology Photosynthesis Packet Review ANSWERS Ask Yourself 1. The pH is lowest in the thylakoid space in a chloroplast. The H+ ions are pumped into the space to set up the concentration gradient. The pH is lowest in the outer compartment in a mitochondrion. The H+ ions are pumped pumped out to the outer compartment to set up a concentration gradient. 2. The H+ ions come from the splitting of H2O by the manganese containing enzyme and obviously from the cytochrome complex pumping H+ ions into the thylakoid lumen. 3. ATP is created by adding an inorganic phosphate to ADP to create ATP. A H+ ion gradient is set up across a membrane which sets up the potential energy to drive chemiosmosis. Chemiosmosis is the movement of H+ ions through ATP synthase. The chemiosmotic energy is couple to the endergonic reaction of smushing a phosphate group onto ADP to create ATP. The only difference between the chloroplast and the mitochondrion is the orientation of the ATP synthase. The head of the lollipop is facing outward toward stroma in the chloroplast and inward toward matrix in the mitochondrion. Testing 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Recall Reduced stores red, blue-violet ATP O2, NADPre, ATP (after H+ gradient) ATP, NADPre PGAL, ADP, NADPox thylakoid membrane, stroma water lower C4 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. PEP (C3) [PEP + CO2 = C4] synthesize carbohydrate D E C B D B D B A (This is a bad question!) B E D E A B C C C (key is – in the reaction centers) A C D C E E B C B A C C D A A B D B B E (Krantz anatomy = mesophyll cells with PEP carboxylase completely surrounding the bundle sheath cells which contain Rubisco and carry out the Calvin cycle.) 52. B