La
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Biology 2430
Molecular Genetics
Instructor: Dr. Mário Moniz de Sá
Lab Demo: Jay Catterson
Office: Room A360
Office: A364
Phone: 604 -323-5976
Phone: 604-323-5338
Email: mmoniz@langara.bc.ca
Office Hours: Mon 12:30 -1:30, Wed 9:30 – 12:30, and Fri 12:302:30
Text:
iGenetics: a Molecular Approach Peter Russel, Third
Edition, 2010 Pearson/Benjamin Cummings.
Study guide and Solutions Manual, Bruce Chase,
20006, Pearson/Benjamin Cummings.
Course Notes:
Follow the links from my website at
http://iweb.langara.bc.ca/biology/mario/
Lab Manual:
Laboratory DNA Science, Bloom, M.V., Freyer, G.A.,
and Micklos, D.A., The Benjamin/Cummings
Publishing Company, Inc. 1996.
Mark Schedule: Quizes
15%
Lecture Exam I
15%
Lecture Exam II
15%
Final Lecture Exam
25%
Lab Assignments and problem sets: 30%
Grade Distribution:
GRADE
A+
A
AB+
B
BC+
C
CD
F
PERCENTAGE %
90-100
85-89
80-84
77-79
73-76
70-72
65-69
60-64
55-59
50-54
0-49
La
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Biol 2430 Course outline
Week
of
Chapt
Lab
Introduction
The Genetic Material
1
2
Jan 11
DNA Replication
3
Lab 1: Measurements,
Micropipetting, Sterile techniques
Lab 2: Isolation of individual
colonies
Lab 3: DNA restriction and
electrophoresis
Jan 18
Gene Function
Transcription
4
5
Jan 25
Translation
Midterm I
6
Feb 1
Recombinant DNA Technology
10
Feb 8
Recombinant DNA Technology
10
Mar 1
Application of Recombinant DNA
Technology
Spring Break Feb 19 and 20
Application of Recombinant DNA
Technology
Midterm II
10
Jan 4
Mar 8
Lecture topic
10
Mar 15
Genomics
9
Mar 22
Mutation and Transposable
elements
7
Mar 29
Regulation of Gene
Transcription in Prokaryotes
Regulation of Gene
Transcription in Eukaryotes
• Genetics of Cancer
Study day
• Final Examinations
Apr 5
Apr 12
Apr 19
Apr 2028
Spring Break
Easter Friday
Easter Monday
Feb 12-26
April 2
April 5
17
Lab 3: con’t
Lab 4: Effects of methylation on
DNA
Lab 4: con’t
Lab 5: Transformation of
plasmid DNA into E. coli
Lab 6A: Purification and
identification of plasmid DNA
Lab 6B: Restriction analysis of
purified plasmid.
Lab 7A & 7B: Recombination of
Antibiotic-resistance genes
Lab 7B: con’t
Lab 8: Transformation of E. coli
with recombinant DNA
Lab 9: Replica plating to identify
mixed E. coli populations
Lab 10A: Purification &
identification of recombinant
DNA
Lab 10B: Restriction analysis of
purified recombinant plasmid
DNA
Lab 10B: con't
Lab 18: PCR amplification of l
fragment
Lab 18: PCR con’t
18
20
No Classes, College Open
College Closed
College Closed