Product Datasheet Cobalt Quantification Kit ab102510 1 Image Overview Product name Cobalt Quantification Kit Tests 100 x 1 assay Sample type Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts Assay type Quantitative Sensitivity > 0.18 µg Range 0.18 µg - 3 µg Assay time 0h 20m Product overview Abcam's Cobalt Quantification Kit takes advantage of the reaction of Co2+ with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe2+, Cu2+, Ni2+, Zn2+, Mn2+, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co2+ in a variety of samples. Visit our FAQs page for tips and troubleshooting. Notes Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co2+ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. Tested applications Functional Studies Properties Storage instructions Store at -20°C. Please refer to protocols. Components Identifier 100 tests Cobalt Chloride Standard Yellow 1 unit Cobalt Reagent Red 1 x 1ml Relevance Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co+2 species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. Applications Our Abpromise guarantee covers the use of ab102510 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Functional Studies Notes Use at an assay dependent dilution. Cobalt Quantification Kit images http://www.abcam.com/Cobalt-Quantification-Kit-ab102510.html 1 Product Datasheet Cobalt Standard Curve: Assays were performed following the kit protocol. Functional Studies - Cobalt Assay Kit (ab102510) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors Visit us at: www.abcam.com http://www.abcam.com/Cobalt-Quantification-Kit-ab102510.html 2