NBIO 401 Fall 2012 Limbic System Class 26 – Monday, November 26, 2012 Robinson Objectives: -Be able to describe the major inputs and outputs, function, and the consequences of lesions or electrical stimulation for each of the six components of the limbic system that we described (i.e., hypothalamus, amygdala, hippocampus, cingulate cortex, nucleus accumbens, and septal nuclei) -Be able to describe the basic interconnections of the limbic system, and what fibers are in the three main fiber tracks described in the lecture: 1) the stria terminalis, 2) the fornix, and 3) the medial forebrain bundle. -Be able to describe the location of these tracks in the brain. -Be able to explain the three ways in which it hypothalamic activity can act to maintain homeostasis. -Be able to explain the different mechanisms through which the hypothalamus controls the anterior and posterior pituitary gland. -Know the two functions of cortical input to the limbic system that we discussed: 1) Recognizing potential threats to homeostasis or well being that the brain can, at least partially, counteract with autonomic adjustments (e.g., seeing a car careening toward you eliciting sympathetic activity to speed your escape). Such threats are abstract in the sense that they do not directly challenge homeostasis as would dehydration or hypothermia. The cortex, with its large processing power, is necessary to recognize these threats from visual and auditory stimuli as opposed to internal conditions directly sensed by the hypothalamus. 2) Restraining or modifying behavior motivated by the limbic system. Phineas Gage (see p. 12) makes this point well. Without the use of his frontal cortex he, among other deficits, acted more directly on his urges and drives than was socially acceptable. ______________________________________________________________________________________________________________ In the previous lectures we have described selected sensory and motor systems that are very capable and flexible, i.e., you can do a very wide variety of things with them. Of the many behaviors available to you, which do you actually choose to exhibit in any situation? This lecture and the one on ascending modulatory systems deal with the parts of the brain that provide motivation for appropriate behavior. The limbic lobe is a term first used by Broca in 1878 for the ring of cortex visible on the medial surface of each hemisphere. As Figure 1 shows, the limbic lobe is composed of the cingulate cortex, medial temporal cortex, and the orbital cortex (the cortex near the bony sockets, or orbits, holding the eyes) directly above the rostral end of the temporal cortex. The olfactory bulbs stick out from this ring like the handle of a tennis racket. This ring cortex marks the medial edge of cortex on the hemisphere, thus its name. Limbic is from the Latin "limbus" for border. -Class 26, page 1- NBIO 401 Fall 2012 Anatomical connections and the behavioral consequences of lesions indicate that other structures work with the cortex of the limbic lobe. Today, the cingulate cortex of the original "limbic lobe" and the subcortical structures associated with it are called the limbic system. The limbic system mediates the two components of emotional responses, 1) the perceived feeling of the emotion and 2) the changes in your body that occur to prepare for the appropriate behavior (e.g., moving blood to the muscles, dilating the pupils). The limbic system includes the following six brain structures: 1) hypothalamus, 2) amygdala, 3) hippocampus, 4) cingulate cortex, 5) nucleus accumbens, and 6) septal nuclei. Below we will describe the location, anatomical connections, and the function each of these structures. The hypothalamus, amygdala, cingulate cortex, and hippocampus are all composed of numerous subdivisions which have distinct connections and functions. For our purposes here we will describe the inputs and functions of the whole structure, not its individual parts. 1) HYPOTHALAMUS Location - The hypothalamus is a relatively small region (weighing only about 4 grams in humans) lateral to and ventral to the walls of the third ventricle near the midline. As shown in Figure 2, the hypothalamus is ventral to the anterior medial part of the thalamus and dorsal and just caudal to the optic chiasm. Connections, Input - The hypothalamus receives input from other limbic structures, the amygdala, the hippocampus, and the septal nuclei as well as from the brainstem and spinal cord. - The input from the amygdala arrives via two pathways, a) the stria terminalis (green line labeled ST in Figure 2), a fiber bundle that travels posterior and medial from the amygdala inside the temporal lobe, away from the temporal pole by the hippocampus, and arches over the thalamus and under the corpus callosum. It then turns ventrally in front of the thalamus and travels down to the hypothalamus. b) the other pathway travels directly dorsal and medial from the amygdala directly to the hypothalamus. -The input from the hippocampus arrives via the fornix (dark blue line labeled “from hippocampus in Figure 2), a fiber bundle that travels posterior and medial from the hippocampus, buried inside the medial part of the temporal lobe and arches over the thalamus above the stria terminalis and under the corpus callosum. The fornix turns ventrally in front of the thalamus and travels down to the mammilary bodies. -Class 26, page 2- NBIO 401 Fall 2012 -Input from the septal nuclei arrives via the medial forebrain bundle, a fiber bundle running in the medial and ventral part of the brain from the septal nuclei, through the lateral hypothalamus and into the rostral medulla. (As apparent in Figure 2, the septal nuclei are located just off the midline on the roof of the lateral ventricle under the corpus callosum. They are dorsal and rostral to the hypothalamus.) Figure 3 shows the general location of the medial forebrain bundle in a parasagittal section of a rat brain. The medial forebrain bundle carries fibers traveling in both directions and is structured like a frayed rope with fibers entering and leaving it at all levels. Some fibers in the medial forebrain bundle travel only short distances, some travel a long way. Fibers leave the septal nuclei and travel ventral and caudal to enter the hypothalamus. There is also a small input from the brainstem and spinal cord, reaching the hypothalamus via the medial forebrain bundle. Output We can divide the output of the hypothalamus into four categories: a) output that goes back to the amygdala (via the stria terminalis), hippocampus (via the fornix), and septal nuclei (via the medial forebrain bundle) traveling in the reverse direction along same pathways via which input from these structures reached the hypothalamus b) output to autonomic centers in the brainstem and spinal cord traveling to the brainstem via the medial forebrain bundle (light blue in Figure 4) c) output, both neural and humoral (relating to bodily fluids), to the pituitary gland (two red arrows in the bottom center of Figure 4) d) output via the medial forebrain bundle to the anterior part of the thalamus which, in turn, projects to the prefrontal, orbital, and cingulate regions of the cerebral cortex (dark blue arrow in the center of Figure 4). -Class 26, page 3- NBIO 401 Fall 2012 Function - The hypothalamus has two basic functions, maintaining homeostasis and preparing the body for emotional responses. It may also play a role in relaying signals about the state of the body relevant to emotional response to other limbic structures. HOMEOSTASIS The hypothalamus maintains critical features of the environment inside the body, such as temperature, salinity, and glucose concentration, within narrow healthy limits. It receives information about the state of the body via input from the brainstem and spinal cord and from neurons inside the hypothalamus. These neurons sense temperature, blood salinity, blood glucose concentration, and temperature among other values. When one of these values deviates from a narrow zone the hypothalamus elicits responses to move it back into the acceptable range. It does so via three mechanisms: a) The hypothalamus mediates changes in the body with the autonomic nervous system. Seen very crudely, anterior hypothalamic regions elicit parasympathetic activity and posterior hypothalamic regions elicit sympathetic activity. For example, if blood salinity raises above its normal value, then the neurons in the hypothalamus that are sensitive to blood salinity will increase their firing rate. Figure 5 shows an example of a hypothalamic neuron that increases its firing rate when blood salinity rises above its ideal level and decreases when it falls below that level. The response of hypothalamic neurons to increased salinity reduces water loss from the body by causing a decrease in parasympathetic drive to reduce sweating and saliva production. b) The hypothalamus modulates the output of the pituitary gland. The hypothalamus controls the posterior pituitary with neural signals. It controls the anterior pituitary with humoral signals. 1. posterior pituitary – As illustrated in Figure 6A, neurons in the hypothalamus send their axons down to the posterior lobe of the pituitary (also called the neurohypophysis). The terminals of these axons release these peptides into a capillary bed in the posterior pituitary via which these substances enter the blood. For example, when blood salinity raises above its acceptable value the hypothalamus, in addition to its autonomic actions, also releases antidiuretic hormone(ADH) into the blood to reduce the amount of water that the kidneys pull out of the blood to reduce water loss. 2. anterior pituitary – As shown in Figure 6B, neurons in the hypothalamus send their axons to terminate in a capillary bed inside the hypothalamus. The terminals of these axons send releasing factors into the capillary bed and the blood containing these factors then travels down the stalk of the pituitary (called the infundibulum) into the anterior -Class 26, page 4- NBIO 401 Fall 2012 pituitary (also called the adenohypophysis). Cells in the anterior pituitary sensitive to a particular factor will release a hormone into the blood if it receives that factor from the hypothalamus. The hormones released from the anterior pituitary gland control the endocrine system and are not directly related to maintaining internal homeostasis. c) The hypothalamus elicits motivation for behavior to restore the errant variable to its accepted value. For example, when blood salinity raises above its acceptable value the hypothalamus elicits drinking by making us thirsty. Creating the appropriate motivation is usually the most effective solution to the problem of an internal value moving out of its normal range. Drinking water is much more effective in reducing salinity than reducing urine production. The hypothalamus elicits motivational states via its ascending projections to the thalamus. Experimental manipulation of particular parts of the hypothalamus can badly impair the regulation of these motivational states. For example, bilateral lesions of the lateral hypothalamus result in overeating and extreme weight gain. Electrical stimulation of different hypothalamic regions can elicit eating or drinking or stop eating or drinking in progress. It can also elicit increases or decreases in body temperature. PREPARING THE BODY FOR EMOTIONAL RESPONSES In addition to changing the body to maintain homeostasis the hypothalamus elicits autonomic changes to prepare the body for emotional responses. For example, if you see a car careening toward you the stimulus is abstract in the sense that, by itself, it causes no change in your temperature or other internal conditions. Yet your cerebral cortex evaluates the significance of the stimulus and, via your hypothalamus, causes strong sympathetic activation that releases adrenaline, increases blood flow to your skeletal muscles, and the dilates your pupils. Descending influences originating in the cerebral cortex and traveling through limbic structures reach the hypothalamus to cause appropriate changes based on external stimuli. Relaying signals about the state of the body to other limbic structures Signals about emotional responses also travel from the hypothalamus to other limbic structures. The James - Lange theory of emotions proposes that we feel an emotion only after our body has reacted to some situation. We are, in effect, observing and responding to the condition of our body. Many observations have led to skepticism about this theory in its entirety. For example feelings can arise faster than, and outlast, the physiological changes that accompany them. Nonetheless, it is also clear that feedback about the state of our body plays a role in emotional feelings. For example, patients with severed spinal cords experience a reduction in the intensity of their emotions. The hypothalamus is in a position anatomically to relay emotion-related information from the brainstem and spinal cord to other parts of the limbic system. -Class 26, page 5- NBIO 401 Fall 2012 2) AMYGDALA Location – As illustrated in Figure 7, the amygdala (Latin for almond because of its shape) is a round nucleus roughly the size of a large olive embedded in the dorsomedial part of the temporal lobe about 3/4 of the way toward the temporal pole. Connections, Input As the left side of Figure 8 illustrates, the amygdala receives input from orbital, cingulate, entorhinal, and temporal cortex. It also receives input from subcortical structures, the hypothalamus, hippocampus, brainstem, septal nuclei, and thalamus. -Class 26, page 6- NBIO 401 Fall 2012 Connections - Output – The right half of Figure 8 shows that the amygdala projects to orbital, cingulate, temporal, and entorhinal cortex. It also projects to subcortical structures, the hypothalamus (both directly and via the stria terminalis), the hippocampus, the septal nuclei and the thalamus. Function In general the amygdala represents an interconnection between the cortex and the hypothalamus. The amygdala’s connections to the cortex probably mediate the feeling of emotions. Electrical stimulation of the amygdala in humans can elicit a variety of emotions but most commonly fear. In animals such stimulation most commonly elicits rage, defensive postures and behavior, and fleeing. Bilateral lesions of the amygdala result in decrease in aggression and generally docile animals. They are unable to retrieve or learn associations between stimuli (e.g., an aggressive enemy) and emotional response (fear, flee now!). The amygdala’s connections to the hypothalamus represent higher level control of the hypothalamus. Stimulation of the amygdala can elicit the same drive-related behaviors elicited by stimulating the hypothalamus directly, e.g., eating or drinking, but the effect is more natural in that it has slower onset and offset. Bilateral amygdala lesions can cause the same deficits as those of the hypothalamus but less severe. 3) HIPPOCAMPUS Location As you can see in Figure 9, the hippocampus is an approximately tubeshaped structure roughly the size of your little finger. It is inside the temporal lobe and curves posterior, dorsal, and medial moving from the amygdala away from the temporal pole. -Class 26, page 7- NBIO 401 Fall 2012 Connections - Input – Figure 10 shows the inputs to the hippocampus. Chief among these is the entorhinal cortex. The entorhinal cortex is that on the ventromedial surface of the temporal lobe. It receives input from other cortical areas, cingulate, orbital, and prefrontal. The hippocampus also receives direct input from the amygdala and hypothalamus (via the fornix). Connections - Output - The hippocampus projects to the anterior thalamus which, in turn projects to prefrontal, orbital, and cingulate cortex. It also projects to other limbic structures, the amygdala and the hypothalamus (via the fornix). (FIGURES 12 & 15) Function - The hippocampus mediates the formation of new declarative memories. A declarative memory is one of a fact that we can express or declare clearly. (This is as opposed to motor learning like that done by the cerebellum, or emotional learning like that done by the amygdala). Bilateral lesions of the hippocampus damage a patient’s ability to form new memories after the lesion. Memories formed before the lesion are fine. For example, a patient with bilateral hippocampus lesions will always feel as if she is meeting her neurologist for the first time even after hundreds of meetings. This deficit is specific. Patients with bilateral hippocampus lesions will improve their ability to do a jigsaw puzzle as they do it over and over though they never remember seeing it before. -Class 26, page 8- NBIO 401 Fall 2012 4) CINGULATE CORTEX Location - The cingulate cortex is on the medial surface of the cerebral hemisphere above the corpus callosum (e.g., Figures 1, 8, & 10). Connections - Input Cingulate cortex receives input from VP thalamus (remember, the one that relays somatosensory information to the cerebral cortex) carrying pain information, and from the anterior thalamus. It also receives input from the amygdala. Connections - Output Cingulate cortex projects to entorhinal cortex and amygdala. Function - Cingulate cortex helps mediate the emotional response to pain and to more abstract stimuli. 5) NUCLEUS ACCUMBENS Location - The nucleus accumbens (or nucleus accumbens septi) is often referred to as the emotional component of the basal ganglia, and it is found where the head of the caudate meets the anterior portion of the putamen (Figure 11A) just lateral to the septum pellucidum. The nucleus accumbens (septi) comes from the Latin phrase meaning “the nucleus leaning against the septum” Connections – Inputs – The nucleus accumbens receives input from the amygdala. Connections – Outputs – Axons of neurons in the nucleus accumbens terminate within the basal ganglia, indicating that the nucleus accumbens represents a link between the limbic system and the basal ganglia. Function - The nucleus accumbens plays an important role in modulating motivation and reinforcement. Particularly, this area appears to be the site of action for many addictive drugs such as cocaine and amphetamines. Cocaine and amphetamines increase dopamine levels in the nucleus accumbens and administration of dopamine antagonists to this nucleus can nullify the pleasurable aspects of drug use. -Class 26, page 9- NBIO 401 Fall 2012 6) SEPTAL NUCLEI Location - As you can see in Figure 11B, the septal nuclei are located near the midline on the floor of the lateral ventricle just above the anterior commissure. They are on the medial wall of the cerebral hemisphere just beneath the base of the septum pellucidum. They form the floor of the medial portion of the lateral ventricles and are found just anterior to the hypothalamus. They are dorsal and rostral to the hypothalamus. Connections – Inputs – The septal nuclei receive a large input from the hippocampus via the fornix, and a smaller projection from the amygdala and the medial part of the midbrain (mesencephalic) reticular formation. Connections – Outputs – The axons of neurons in the septal nuclei travel via the fornix back to the hippocampus, via the stria terminalis to the hypothalamus, and via the medial forebrain bundle to the amygdala. Function - The septal nuclei seem related to the experience of pleasure. Excitation of this area in humans results in extreme feelings of pleasure and joy. Animals will lever press repeatedly to obtain electrical stimulation of this area, to the point of forgoing food and sleep. Moreover, electrical recording from this area registers increased activity during sexual orgasm. However, the septal nuclei do not appear to be solely involved in feelings of pleasure. They also represent important cholinergic inputs (further discussed in Lecture 30) to the hippocampus and may play a role in modulating memory function. Interestingly, in certain animals, damage to this area results in a permanent condition known as ‘sham rage’animals become incredibly angry/violent. In addition to knowing about the six components of the limbic system that we have reviewed above you may be interested in two other topics related to the limbic system, Papez Circuit and the emotional function of the prefrontal cortex as demonstrated by damage to it. We discuss each of these topics on the following two pages. Finally, on page 13 there is a schematic of the connections between different limbic structures. There is a lot of information in this schematic and you are responsible for only those facts listed above. The most important facts are the connections of each of the three major fiber bundles and the connections of the hypothalamus to the pituitary gland. -Class 26, page 10- NBIO 401 Fall 2012 Figure 12 PAPEZ CIRCUIT In the 1930s an American psychologist, James Papez, proposed that an interconnected group of structures in the brain controlled the experience and expression of emotions. These structures form a circuit that is called Papez Circuit (illustrated in Figure 12). We can begin tracing this circuit at the cingulate cortex. It projects to the entorhinal cortex which projects, in turn, to the hippocampus. The hippocampus projects, via the fornix, to the mammilary bodies of the hypothalamus and other parts of the hypothalamus (not shown in Figure 16). The hypothalamus projects to the anterior nucleus of the thalamus which projects to the cingulate cortex, completing the circuit. Papez believed that areas of the neocortex outside the circuit helped modulate emotion because damage to the cortex can disturb emotions (e.g., Phineas Gage). Papez also proposed that activity in other neocortical areas caused by signals from the cingulate cortex added nuance or "emotional coloring" to the experience of emotion. Evidence indicates that some of the structures in Papez circuit mediate emotion. For example, tumors in the cingulate cortex are often associated with excess fear and irritability. Damage to the anterior thalamus can cause spontaneous crying or laughing. Papez thought that the hippocampus was involved in emotion because rabies infections cause both clear abnormalities in hippocampal neurons and hyper-emotional responses like exaggerated fear and aggressiveness. We now know that the hippocampus primarily mediates declarative memory. The amygdala, not part of Papez circuit, is probably more linked to emotional responses than is the hippocampus. From a modern perspective we can see Papez circuit as an early, and reasonably accurate, attempt to identify the brain regions involved in emotion. We have much more data than Papez did and, because of this, have a more specific idea about what each limbic structure does. -Class 26, page 11- NBIO 401 Fall 2012 Frontal Cortex and Emotion A dramatic, if crude, demonstration that prefrontal influences emotion occurred when, in 1848, an 25 year old construction foreman, Phineas Gage, working on a railroad in New Hampshire was the victim of an industrial accident. He was tamping explosive powder into a hole bored into a large rock in preparation for blowing part of the rock away to clear the path for a railroad. He looked away and his tamping bar struck the rock and made a spark that ignited the explosive. The explosion drove Gage’s tamping rod, which was over a meter long and about 2 inches in diameter, upward into his left cheek and out the top of his skull, destroying a large part Figure 13 of his frontal cortex bilaterally. Figure 13 shows the position of the tamping rod in Gage’s skull. Gage survived this horrible injury and the major infection that followed it but his personality was permanently changed. Once calm, productive, and organized he became capricious, unable to plan toward goals, profane, easily distracted, impatient of advice or restraint, and generally unrestrained in trying to satisfy his drives, eating and drinking to excess and seeking sex constantly and with little finesse. Many of these same characteristics are exhibited by people receiving frontal lobotomies. Surgery consisted of severing many of the fiber connections between the frontal lobes and the rest of the brain using a stiff, narrow probe inserted into the brain trough a hole in the bone above the eye as shown in Figure 13. These surgeries were performed on people with a variety of complaints, including depression and anxiety. While their IQ did not suffer greatly, patients receiving frontal lobotomies commonly exhibit blunting of emotions and loss of the emotional component of thoughts. They, like Phineas Gage, often developed "inappropriate behavior", or, as it was characterized at the time, an apparent “lowering of moral standards”. Also like Gage, these patients were easily distracted and had considerable difficulty planning and working toward goals. -Class 26, page 12- NBIO 401 Fall 2012 -Class 26, page 13-