Sartobind® IEX nano 3 mL, 150 mL, 1.2 L, 5 L, 8 mm bed height
Process-Scale Membrane Adsorbers Ion Exchange
Applications
Polishing
– Virus clearance >6 log
Membrane Chromatography
– DNA below detection limit
– Endotoxins >5 log
– Host cell proteins >99%
– Leached ligand materials
– Aggregates
Capture
– Large proteins
– Viruses
– Vaccines
– Proteins from large feed streams
Sartobind strong cation (S) and anion ligands
(Q) are covalently attached as a flexible
hydrogel onto the stabilized reinforced
­cellulose. The membrane pore size of >3 µm
allows large proteins, bioparticles and viruses
to enter the macroporous structure and
achieve high binding capacity without size
exclusion effects.
E280 nm
The membrane is rolled up to form
a cylinder with a bed height of 8 mm
around a central solid core.
The optimized design of the fluid channels
reduces the void volume significantly,
resulting in sharp breakthrough curves
with minimal elution volumes. The recommended flow rate of Jumbo 5 L is 25 liters per
minute.
The internal support structures and the
outer shell of the Sartobind Jumbo are made
from polypropylene.
Sartobind nano 3 mL is the recommended
scale down model for the Sartobind Jumbo.
The membrane geometry and the materials of
construction are identical in all three devices.
3
60
2.5
50
2
40
1.5
30
1
20
0.5
10
0
Conductivity [mS/cm]
The Sartobind Q or S capsules with 8 mm bed
height are membrane chromatography devices for large scale capturing and impurity
removal at high flow rates.
0
0
50
100
150
Volume [L]
200
250
Bovine serum albumin (BSA) 2 g/l was loaded onto a 5 L Sartobind Q Jumbo. Dynamic binding
capacity at 10 % breakthrough was achieved at ~53 liters, equivalent to 106 g of protein.
Elution volume accounted for 10 liters, equivalent to only 2 membrane volumes.
Benefits
– Designed for large scale flow through
polishing as well as bind and elute
chromatography
– Small elution volumes at 2 membrane
volumes
– 8 mm bed height for increased binding
capacity
– Scale down model with Sartobind nano
3 mL with 8 mm bed height
– Recommended flow rate of 5 bed volumes
per minute
– Easy and simple handling (like a filter)
– Autoclaveable or CIP with 1 N NaOH 1 h
Technical Data
Description
Sartobind Q nano 3 mL
Sartobind S nano 3 mL
Sartobind Q 150 mL
Order number
96IEXQ42EUC11--A
96IEXS42EUC11--A
96IEXQ42E9BFF Shipment
4 + Sartobind Q nano 3 mL, 4 + Sartobind S nano 3 mL, Sartobind Q 150 mL, 2 Luer male to UNF 2 Luer male to UNF manual, certificate
10–32 adapters PEEK, 10–32 adapters PEEK, manual
manual
Membrane material
Stabilized reinforced cellulose
LigandStrong basic anion Strong acidic cation Strong basic anion exchanger:
exchanger:
exchanger:
quaternary ammonium sulfonic acid quaternary ammonium (R-CH2-N+(CH3)3)
(R-CH2-SO3-)
(R-CH2-N+(CH3)3)
Pore size
>3 µm
>3 µm
>3 µm
Bed height
8 mm
8 mm
8 mm
Bed volume
3 mL
3 mL
150 mL
Nominal adsorption area
110 cm2
110 cm2
5500 cm2
Typical dyn. binding capacity 10%
– per cm2
0.8 mg BSA
0.7 mg lysozyme
0.8 mg BSA
– per ml
29 mg
25.5 mg
29 mg
– per unit 88 mg
77 mg
4.4 g
Recommended flow rate
0.015 L/min
0.015 L/min
0.75 L/min
Void volume*
4 mL
4 mL
0.2 L
Maximum pressure
0.4 MPa (4 bar, 58 psi)
0.4 MPa (4 bar, 58 psi)
0.4 MPa (4 bar, 58 psi)
Housing material
Polypropylene
Polypropylene
Polypropylene
Weight
10 g
10 g
400 g
Connectors
Luer female
Luer female
Sanitary 25 mm 3"
pH stability (short term)
2 –14
3 –14
2–14
pH stability (long term)
2 –12
4 –13
2–12
* Includes the porosity of the membrane which is approximately 80%.
Sartobind S 150 mL
96IEXS42E9BFF
Sartobind S 150 mL,
manual, certificate
Strong acidic cation
exchanger:
sulfonic acid
(R-CH2-SO3-)
>3 µm
8 mm
150 mL
5500 cm2
0.7 mg lysozyme
25.5 mg
3.9 g
0.75 L/min
0.2 L
0.4 MPa (4 bar, 58 psi)
Polypropylene
400 g
Sanitary 25 mm 3"
3–14
4–13
Technical Data
Description
Sartobind Q 1.2 L
Sartobind S 1.2 L
Order number
96IEXQ42E3FSS
96IEXS42E3FSS
Shipment
Sartobind Q 1.2 L
Sartobind S 1.2 L
manual, certificate
manual, certificate
Membrane material
Stabilized reinforced cellulose
LigandStrong basic anion
Strong acidic cation
exchanger:
exchanger:
quaternary ammonium
sulfonic acid
(R-CH2-N+(CH3)3)
(R-CH2-SO3-)
Pore size
>3 µm
>3 µm
Bed height
8 mm
8 mm
Bed volume
1.2 L
1.2 L
Nominal adsorption area
4.4 m2
4.4 m2
Typical dyn. binding capacity 10%
– per cm2
0.8 mg BSA
0.7 mg lysozyme
– per ml
29 mg
25.5 mg
– per unit
35 g
31 g
Recommended flow rate
6 L/min
6 L/min
Void volume*
1.6 L
1.6 L
Maximum pressure
0.4 MPa (4 bar, 58 psi)
0.4 MPa (4 bar, 58 psi)
Housing material
Polypropylene
Polypropylene
Weight
1.9 kg dry
1.9 kg dry
Connectors
Sanitary 50.5 mm 11”
Sanitary 50.5 mm 11”
pH stability (short term)
2 –14
2–14
pH stability (long term)
2 –12
2–12
* Includes the porosity of the membrane which is approximately 80%.
Accessories for Jumbo
Order number
Description
9ZGL- -0102Trolley for Jumbo 5 L,
stainless steel
16290
Sartocheck® 3 Plus
26288
Sartocheck® 4 Plus
Sartobind Q Jumbo 5 L
Sartobind S Jumbo 5 L
96IEXQ42E3ESS
Sartobind Q Jumbo 5 L, 15 membrane discs d 30 mm, manual, certificate
96IEXS42E3ESS
Sartobind S Jumbo 5 L,
15 membrane discs
d 30 mm,
manual, certificate
Strong basic anion exchanger:
quaternary ammonium (R-CH2-N+(CH3)3)
>3 µm
8 mm
5 L
18.2 m2
Strong acidic cation
exchanger:
sulfonic acid
(R-CH2-SO3-)
>3 µm
8 mm
5L
18.2 m2
0.8 mg BSA
29 mg
145 g
25 L/min
7 L
0.3 MPa (3 bar, 44 psi)
Polypropylene
16 kg dry, 20 kg wet, 23 kg filled
Sanitary 50.5 mm 11"
2–14
2–12
0.7 mg lysozyme
25.5 mg
127 g
25 L//min
7L
0.3 MPa (3 bar, 44 psi)
Polypropylene
16 kg dry, 20 kg wet,
23 kg filled
Sanitary 50.5 mm 11"
3–14
4–13
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
37079 Goettingen, Germany
Phone +49.551.308.0
Fax +49.551.308.3289
www.sartorius-stedim.com
USA Toll-Free +1.800.368.7178
UK +44.1372.737159
France +33.442.845600
Italy +39.055.63.40.41
Spain +34.90.2110935
Russian Federation +7.812.327.5.327
Japan +81.3.4331.4300
Specifications
subject to
to change
change
Specifications subject
without
notice. Printed
Printed and
and copyrighted
copyrighted
without notice.
by
Sartorius
Stedim
Biotech
GmbH. || W
W
by Sartorius Stedim Biotech GmbH.
Publication
No.:
Publication No.: SL-2053-e13068
Order
Order No.:
No.: 85034-535-76
Ver.
| 2012
Ver. 01
08 | 2013