LESSON 5: Decomposers in Different Environments -- Scientific Method GRADE: 2 LEARNING TARGET: Compare the growth of decomposers in different environments. • • GLE: 2.2.3 Understand that similar investigations may not produce similar results. o Observe the procedure of two similar investigations and explain that they produced different results. GLE: 2.2.5 Know that ideas in science change as new scientific evidence arises. o Tell how scientific inquiry results in facts, unexpected findings, ideas, evidence, and explanations. LESSON OBJECTIVES: • Groups of students will conduct mini-experiments to compare the growth of some decomposers. • Groups of students will practice the scientific method. PROCESS SKILLS: ACTIVITY TYPE X Science X Required Human Endeavor Content Integration Inquiry Lesson Optional Individual or Extended Reading Connection X Cooperative Group Project Writing Prompt Research Project Hands-on Science Activity Culminating Project Computer Lab Activity KIT MATERIALS • • • 30 Hand Lenses • 1 Spray bottle 30 Self-closing bags • 1 Teacher knife Journal Pages: What • 1 spoon Rot! (4) • 6 cotton balls *The following items need to be ordered at 8479. Allow 2-3 days for delivery please: • 2 Slices of bread • 1 Banana • 1 Citrus (lemon or orange) • 2 packages of Yeast NON-KIT MATERIALS Refrigerator or other cool, dark place LESSON 5: Decomposers in Different Environments -- Scientific Method VOCABULARY: GRADE: 2 bacteria – one celled organism which can be seen only with a microscope; some types of bacterium cause decay, others cause disease. fungi – a large group of non-green plants that produce by spores decomposer – an organism that breaks down cells of dead plants and animals into simpler substances mold – a downy or furry growth on the surface of organic matter TEACHER BACKGROUND: Fungi and bacteria are essential to the decay of plant material. Fungi (including molds) and bacteria are decomposers working at the microscopic level to help plants break down into their basic elements. Moisture and darkness encourage rapid growth of fungi and some bacteria. Both of these factors help fungi and bacteria thrive. Set up your observations on a Friday and view the following M-F. This will give a couple of days for things to start growing. Preparation: • Three days in advance order fresh items from 8479. • One day in advance of the lesson, cut the citrus into quarters and rub each of them on the floor to pick up bacteria. Let the quarters sit out overnight. • Arrange to have a cool dark place to store several filled self-closing bags for one week. A refrigerator is a good place. • Locate a warm dark place to store several filled self-closing bags for one week. A closet or covered box near a working heater is a good place. • Put water in the spray bottle. • If you have less than 30 students in your class, invite another class to participate in a cooperative science project. This will give you the multiple tests necessary to validate a scientific experiment. Even numbers in the groups are easiest to cope with. LESSON 5: Decomposers in Different Environments -- Scientific Method GRADE: 2 PROCEDURE HELPFUL HINTS 1. Ask the class how they think plants decompose and what factors effect decomposition. 2. Do things just decompose by themselves or do they have help doing it? Discuss various ideas. 3. What conditions do they think are the best to help decomposers grow? Accept various ideas. 2. Actually, no. Without various decomposers, (many of which we cannot see without a microscope) things would NOT decompose! 4. Explain they are going to test which environmental conditions decomposers like mold, fungi and bacteria will grow in the best. 4. Fungi, molds and bacteria are not the only decomposers! 5. Divide the class into 5 groups. Even numbers are best. To each student in the group give a self-closing bag. 5. Try to have 6 students per group; four is an absolute minimum. If your class is too small invite another 2nd grade class to participate to get the multiple tests required for the scientific method. 6. On a piece of masking tape, instruct each student to write the group number and their name and place the tape on the bag. 7. Inform the class that each group will be conducting a mini-experiment. Each group will write its own question to answer with an experiment. All of us together are exploring which conditions a decomposer (mold, fungi and bacteria) will grow in the best. 8. Into the bag o Group 1 will place a half slice of bread o Group 2 will place a half slice of bread sprayed with two squirts of water from the spray bottle. o Group 3 will place a one-inch slice of banana and ½ spoonful of yeast. o Group 4 will place a quarter of citrus in the bag. o Group 5 will place a quarter of citrus in the bag along with a cotton ball moistened with water. 9. Instruct the groups to zip the bags shut. Leave air in the bags. 8. o Inform group 4 and 5 that yesterday you rubbed their citrus quarters on the floor to pick up bacteria. LESSON 5: Decomposers in Different Environments -- Scientific Method PROCEDURE 10. Take a few minutes to discuss the concept of variables with the class. A variable is something that can be changed in an experiment. Science rules say that we can only change one variable at a time. 11. Place half of the bags from group 1,2,3,4, and 5 in a cool dark place. GRADE: 2 HELPFUL HINTS 10. This lesson is really several small experiments combined. Don’t let students get confused! The only variable that we are changing is temperature. 12. Place the other half of the bags from group 1,2,3,4, and 5 in a warm dark place. 13. Help students to write the question they are trying to find the answer to: o Group 1 will ask the question: Will bread mold grow best in warm/dry or cool/dry conditions? o Group 2 will ask the question: Will bread mold grow best in warm/wet or cool/wet conditions? o Group 3 will ask the question: Will fungi (yeast) on a banana grow best in cool or warm conditions? o Group 4 will ask the question: Will bacteria on citrus grow best in warm/dry or cool/dry conditions? o Group 5 will ask the question: Will bacteria grow best in warm/wet, or cool/wet conditions? 14. Have students hypothesize what will happen and record in the space provided on the Journal Page: What Rot! 15. Speculate as a class. What will the yeast do? What affect will temperature have? Is moisture important? 16. Everyday during the week have students observe their group’s bags and carefully record their observations on the Journal Page: What Rot! 16. Have a hand lens available. Keep the bags closed. 17. After one week the bread and banana should be well on their way to decomposing. Allow the students to observe the structures of mold and the decomposition of the bananas. 17. The citrus may take a week or longer to turn into bluish-green fuzz balls. If so, continue the experiment an extra week. 18. Invite students to draw what they see on the Journal Page: What Rot! LESSON 5: Decomposers in Different Environments -- Scientific Method GRADE: 2 PROCEDURE HELPFUL HINTS 19. Instruct students to write a conclusion about their experiment. 20. As a class discuss what has happened in each bag. Did all of the bread, banana, citrus decompose at the same rate? Why or why not? What role did the yeast play? What role did temperature play? What role did moisture play? 21. Were their any surprises? Remind the students that even though an experiment may produce an unexpected result, scientists should report things as they actually happened. No erasing! 21. Unexpected results from experiments have resulted in very exciting information like the discovery of penicillin! 22. Guide students to come to some conclusions about which conditions affect the rate of growth of bread mold, fungus on bananas and bacteria on citrus fruit. 23. Does it appear that decomposers mostly like it warm, moist and dark? 23. Low and behold, those are the conditions of your worm bin! Molds, fungi, bacteria, and worms are all type of decomposers. 24. Decide as a class if you need to repeat this experiment in light conditions instead of in the dark to verify your assumption. 24. Call 8479 for more supplies if you choose to do this again! 25. Toss the still closed bags into the trash. 25. Bread mold produces an antibiotic that is not friendly to worms. Too much citrus in a worm bin also can make worms sick. Bananas and yeast are OK in the bin. 26. Have students wash up.