NMC 48th Annual Meeting January 25-28, 2009 Charlotte, North Carolina Titles of Posters to be Presented at the Technology Transfer Session 1. Probability Distribution for the Causal Pathogen of Clinical Mastitis Cases 1 1 2 1,3 1 Wilma Steeneveld , Linda van der Gaag , Herman Barkema and Henk Hogeveen , Utrecht University, 2 3 Utrecht, The Netherlands, University of Calgary, Calgary, Canada, Wageningen University, Wageningen, The Netherlands 2. The National Cohort of Dairy Farms – A Research Platform for Mastitis Management, Planning and Control in Canada 1,2 1,3 1,3 1,4 1,3 Kristen Reyher , Simon Dufour , Denis Du Tremblay , Herman Barkema , Luc Des Coteaux , 1,5 1,2 1,3 1,3 1 2 Trevor DeVries , Ian Dohoo , Jean-Philippe Roy , Daniel Scholl , CBMRN, Atlantic Veterinary 3 4 5 College, UPEI, University of Montreal Vet Med, University of Calgary Veterinary Medicine, University of 1 2 3 Guelph, Kemptville, St. Hyacinthe, Quebec, Charlottetown, Prince Edward Island, St. Hyacinthe, 4 5 Quebec, Calgary, Alberta, Kemptville, Ontario, Canada 3. Relationship Between Coagulase Negative Staphylococcal species, Milk Somatic Cell Count, and Duration of Intramammary Infection 1 1 2 1 Jeanette Perry , John Middleton , Ruth Zadoks , University of Missouri, Columbia, Missouri, USA, 2 Moredun Research Institute, Edinburgh, Scotland 4. Performance of the Direct Cell Counter on Ovine Milk Samples Carlo Spanu, Yves Berger, Dave Thomas and Pamela Ruegg, University of Wisconsin, Madison, Wisconsin, USA 5. Evaluation of Bulk Tank Goat Milk Quality in California 1 1 1 2 2 3 1 C. Collar , D. Costa Bacon , N. Silva del Rio , E. Maga , J. Murray and J. Cullor , University of 2 California Coop. Ext, Hanford and Tulare, California, USA, University of Californi, Davis, California, USA, 3 School of Vet Medicine, University of California at Davis, California, USA 6. Coliform Mastitis Outbreak Associated with Extended Pirlimycin-based Intramammary Therapy 1,2 1 1 1 Marcos Munoz , Michael Zurakowski , Ynte Schukken , Quality Milk Production Services, Cornell 2 University, Ithaca, New York, USA, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile 7. Preliminary Laboratory Evaluation of the Minnesota Easy Culture System II Tri-Plate and the 3M Petrifilm Staph Express Plate Using Clinical Mastitis Samples 1 1,2 2 1 2 J. McCarron , G. Keefe , D. Poole , Atlantic Veterinary College, Prince Edward Island, Canada, Maritime Quality Milk, Atlantic Veterinary College, Prince Edward Island, Canada 8. On-Farm Culture: Characteristics of the Test 1 2 1 1 1 Martín Pol , Carla Bearzi , Julia Maito , Javier Chaves , Lactodiagnostico Sur, Olivos, Buenos Aires, 2 Argentina, La Polvorilla Dairy Farm, Castelli, Buenos Aires, Argentina 9. On-Farm Culture and Guided Treatment Protocols Ana C. O. Rodrigues, Luiz C. Roma Junior, Talita G. R. Amaral, and Paulo F. Machado, Clinica do Leite, Department of Animal Science, ESALQ-USP, Piracicaba, Sao Paulo, Brazil 10. Effect of the Selective Treatment of Clinical Mastitis Based in On-Farm Culture Results on Clinical Mastitis Recurrence, Somatic Cell Count, Milk Production and Culling 1 1 1 2 3 1 Alfonso Lago , Sandra Godden , Russ Bey , Pamela Ruegg , Ken Leslie , University of Minnesota, St. 2 3 Paul, Minnesota, USA, University of Wisconsin, Madison, Wisconsin, USA, University of Guelph, Guelph, Ontario, Canada 11. Liner Compression Applied to Teats During the ‘Rest-Phase’ of Each Pulsation Cycle: Everything You Always Wanted to Know 1 2 1 2 Graeme A. Mein and Douglas J. Reinemann , Werribee, Victoria, Australia, University of Wisconsin, Madison, Wisconsin, USA 12. Milking Performance of Dairy Goats in Wisconsin 1 2 2 2 2 1 Gema Romero, Lindley Reilly, Romina Panzalis, Marcus Haugen and Pamela Ruegg , Universitas 2 Miguel Hernandez, Orihuela, Spain, University of Wisconsin, Madison, Wisconsin, USA 13. Risk Factors for Recurrence of Clinical Mastitis Pamela Ruegg, University of Wisconsin, Madison, Wisconsin, USA 14. Comparison of Bacterial Load Between Teat Dip Cup Types With or Without Attachment 1 2 1 2 2 1 Elizabeth Berry , Colin Kingston , Jenny Clarke , Richard Hiley , Richard May , Institute for Animal 2 Health, Newbury, Berkshire, United Kingdom, Ambic, Witney, Oxfordshire, United Kingdom 15. Effects of Storage Methods on the Recovery of Mycoplasma species from Milk Samples Sukolrat Boonyayatra, John M. Gay, Thomas E. Besser, Ashish Sawant and Larry K. Fox, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA 16. The Detection of Acholeplasma sp. in Individual Cow Milk Samples as ‘False Positive’ Growth in Mycoplasma Culture Allan Britten, Justine Britten, Nancy Hanson, Dana Andre, Jon Cerar, Udder Health Systems, Bellingham, Washington, USA 17. Serum Acute Phase Protein Concentrations in Dairy Cows With Acute Staphylococcal Bovine Mastitis 1 2 2 1 Jose Fagliari , Luiz Martins-Filho , Ana Vidal-Martins , Sao Paulo State University-UNESP, Campus de 2 Jaboticabal-SP, Brazil, College of Veterinary Medicine-UNIRP-Campus de Sao Jose do Rio Preto-SP, Brazil 18. Real-Time PCR-Based Identification of Bacteria in Milk Samples From Bovine Clinical Mastitis With No Growth in Conventional Culturing M.T. Koskinen, Finnzymes Diagnostics, Espoo, Finland 19. Comparison of Staphylococcus aureus and Streptococcus agalactiae Culture Results From Bulk Milk and Quarter Samples 1 1 2 3 1,4 1 R.G.M. Olde Riekerink , O.C. Sampimon , L. Holst Pederson , J. Katholm , and T.J.G.M. Lam , GD 2 Animal Health Service, Deventer, The Netherlands, The Danish Veterinary and Food Administration, 3 4 Haderslev, Denmark, Danish Dairy Board, Århus, Denmark, Dutch Udder Health Centre, Deventer, The Netherlands 20. Prevalence of Enterotoxin Genes in Staphylococcus aureus Recovered From Cases of Subclinical Mastitis Leane Oliveira, Carol Hulland and Pamela Ruegg, University of Wisconsin, Madison, Wisconsin, USA ....... 21. PFGE Typing of Staphylococcus aureus Isolates From Different Sources in Herds with Mastitis Problems 1,2 1 1 1,2 1 Aldo Capurro , Anna Aspàn , Helle Ericsson Unnerstad , Karin Persson Waller , Karin Artursson , 1 2 National Veterinary Institute, Uppsala, Sweden, Swedish University of Agricultural Sciences, Uppsala, Sweden 22. Comparison of Two Phenotypic Methods for Penicillinase Detection in Staphylococcus aureus Isolated from Bovine Mastitis 1 2 1 3 1 3 Julia Maito , Norma Russi , Liliana Tirante , Marcelo Signorini , Martín Pol , Luis Calvinho 1 2 Lactodiagnostico Sur, Olivos, Buenos Aires, Argentina, Facultad de Ciencias Veterinarias, Universidad 3 Nacional del Litoral, Esperanza, Argentina, EEA INTA, Rafaela, Argentina 23. The Effects of Centrifugation and Resuspension Method on the Recovery of Mycoplasma spp. 1 1 1 1 2 1 V. Punyapornwithaya , L.K. Fox , J.M. Gay , D.D. Hancock and J.R. Alldredge , College of Veterinary 2 Medicine, Department of Statistics, Washington State University, Pullman, Washington, USA 24. Daily Variability in Somatic Cell Count of Dairy Goats and Cows Natália M. Sobreira, Jose C. F. Pantoja and Pamela Ruegg, University of Wisconsin, Madison, Wisconsin, USA 25. Comparing Diagnostic Tests for Antimicrobial Resistance Profiling of Bovine Mastitis Pathogens on Canadian Dairy Farms 1 2 3 3 1 Vineet Saini , Richard GM Olde Riekerink , JT McClure , Doris E Poole , Herman W Barkema 1 2 University of Calgary, Calgary, Canada, GD Animal Health Service Ltd, Deventer, The Netherlands, 3 Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada 26. Accuracy of the Delvotest SP at the Determined MRL Values for Various Antibiotic and AntiInflammatory Drugs 1 1 2 1 S. Friedman , A. Harel , and S. Soback , Israel Dairy Board-National Service for Udder Health and Milk 2 Quality, Caesaria, Israel, Kimron Veterinary Institute, Food Safety Laboratories, Beit Dagan, Israel TM 27. Detection of Mastitis-Causing Pathogens in Mastitic Milk Samples Using DairyGuard 1 1 1 2 3 3 4 4 N. Smit , H. Klapproth , P. Piunno , D. Slavic , R. Card , P. Wakeley , B. Lam , and S. Shamim , 1 2 Safeguard Biosystems, Inc., Toronto, Canada, Animal Health Laboratories, Guelph, Canada, 3 4 Veterinary Laboratory Agency, New Haw, United Kingdom, Norgen Biotek Corp., Thorold, Ontario, Canada 28. Antimicrobial Susceptibility of Streptococcus uberis Isolated in Mastitis Milk in France 1 2 3 1 Olivier Bidaud , Marcel Bonnier , Jantijn Swinkels , Intervet Schering-Plough, Farm and Production 2 Animals Business Unit, Angers, France, Laboratoire d’Analyses d’Ille-et-Vilaine, Rennes, France, 3 Intervet Schering-Plough, Int. Marketing, Boxmeer, The Netherlands 29. Bulk Tank Surveillance Program for New York Dairies: Use of Bulk Milk ELISA to Predict Herd-level Prevalence of Paratuberculosis 1 1 1 2 1 Tonya Van Slyke , Rebecca Quesnell , Fabiola Nunes-Braga , Daryl Nydam , Ruth Zadoks , John 3 1 1 Huntley , and Ynte Schukken , Quality Milk Production Services, Cornell University, Ithaca, New York, 2 3 USA, Dept. of Population Medicine & Diagnostic Sci., Cornell University, Ithaca, New York, USA, New York State Department of Agriculture and Markets, Albany, New York, USA 30. Dairy Producers Profile and Molecular Detection of Staphylococcus aureus in Bulk Tank Milk of Farms from Botucatu Region, São Paulo, Brazil Patrícia Yoshida Faccioli, Maria de Lourdes Ribeiro de Souza da Cunha, Helio Langoni, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil 31. Microbiological and Citological Aspects in Goat Milk Juliana Cristina Cardoso Citadella, Helio Langoni, Daniel da Silva Penachio, Patrícia Yoshida Faccioli, Felipe Laurino, Benedito Donizete Menozzi, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil 32. Etiological Aspects and Quality of Bovine Milk Daniel da Silva Penachi, Helio Langoni, Juliana Cristina Cardoso Citadella, Felipe Laurino, Patrícia Yoshida Faccioli, Benedito Donizete Menozzi, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil 33. Effect of Milk price and Quota on Mastitis Management 1 2 1,3 1 Kirsten Huijps , Theo Lam and Henk Hogeveen , Veterinary Medicine, Utrecht University, Utrecht, The 2 Netherlands, Dutch Udder Health Center at GD Animal Health Service, Deventer, The Netherlands, 3 Wageningen University, Wageningen, the Netherlands 34. Economic Behaviour of Farmers 1 2 1,2 1 Kirsten Huijps , Gerrit Antonides and Henk Hogeveen , Veterinary Medicine, Utrecht University, 2 Utrecht, The Netherlands, Wageningen University, Wageningen, The Netherlands 35. Evaluating Expert Opinions on the Definition of Intramammary Infection Using Conjoint Analysis 1 1 2 1 S. Andersen , I. Dohoo , R. Olde Riekerink , Atlantic Veterinary College, University of Prince Edward 2 Island, Charlottetown, Prince Edward Island, Canada, GD Animal Health Service Deventer, Deventer, The Netherlands 36. Non-aureus Staphylococci – Still Minor Pathogens? 1 1 1 2 1 1 R. Bexiga , M. Oliveira , C. Carneiro , K. Ellis , C. L. Vilela , Faculty of Veterinary Medicine, Technical 2 University of Lisbon, Lisbon, Portugal, University of Glasgow Veterinary Faculty, Glasgow, United Kingdom 37. Stall Management and Coagulase Negative Staphylococcus Infection Incidence , , , Simon Dufour¹ ³, Ian Dohoo² ³, Daniel Scholl¹ ³, ¹University of Montreal, ²Prince Edward Island University, , ³Canadian Bovine Mastitis Research Network, ¹ ³St-Hyacinthe, Quebec, Canada, ²Charlottetown, Prince Edward Island, Canada 38. Premilking and Postmilking Teat Antisepsis in Dairy Farms of Argentina E. Izak and J. C. Bonazza, Mastitis Prevention Services, Buenos Aires, Argentina 39. Keratin Plug Presence in Heifers 60 Days Before Calving and Its Relation With Intramammary Infections 1 1 2 1 Luis Miguel Jiménez , Raquel Timón , Juan C. Marco , Servet Talavera SL, Talavera de La Reina, 2 Toledo, Spain, Laboratorio de Salud Pública, Departamento de Sanidad, Gobierno Vasco, Spain 40. The Ability of Enterococcus spp. to Survive the Ensiling Process S. N. Masiello and C. S. Petersson-Wolfe, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 41. Genetic and Phenotypic Profiles of Klebsiella pneumoniae Isolates of Environmental Versus Clinical Mastitis Origin 1,2 1 1 1 1 Marcos Munoz , Rebecca Quesnell , Ruth Zadoks , Ynte Schukken , Quality Milk Production Services, 2 Cornell University, Ithaca, New York, USA, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile 42. Chronic Escherichia coli Experimental Intramammary Infections in Primiparous Dairy Cows During the Periparturient Period 1 1 1 1 2, 2 2 S.P. Oliver , S.I. Headrick , M.J. Lewis , B.E. Gillespie , L.M. Bauer D.L. Johnson , K.C. Lamar , and 1 1 2 R.A. Almeida , The University of Tennessee, Knoxville, Tennessee, USA, Middle Tennessee Research & Education Center, Spring Hill, Tennessee, USA 43. Correlation Between Somatic Cell Count, Milk Yield and Quality in Five Italian Water Buffalo Dairy Herds 1 2 2 2 2 2 Giuseppe Ronci , Clara Locatelli , Licia Scaccabarozzi , Valerio Bronzo , Antonio Casula , Paolo Moroni , 1 2 DVM, Bovine practitioner, Department of Veterinary Pathology, Hygiene and Public Health, University of Milan, Italy 44. Milk Yield on the Subsequent Lactation Following Selective Treatment of Cows at Dry-Off 1,2 1 1 1 1 Audrey H. Torres , Päivi J. Rajala-Schultz , Fred J. DeGraves and Fernando Silveira , The Ohio State 2 University, Columbus, Ohio, USA, Universidad Centroccidental “Lisandro Alvarado,” Barquisimeto, Lara, Venezuela 45. Epidemiologic Association of Staphylococcus aureus Virulence Markers With Non-Clinical Intramammary Infection 1,3 2,3 1,3 2,3 1,3 1 B.V. LeThanh , C.L. Jacob , S. Messier , F. Malouin , D. Scholl , University of Montreal, Saint2 3 Hyacinthe, Québec, Canada, University of Sherbrooke, Sherbrooke, Québec, Canada, Canadian Bovine Mastitis Research Network, Saint-Hyacinthe, Québec, Canada 46. Acute phase proteins in milk; biomarkers for sub-clinical mastitis and milk quality? 1 2, 1 1 Maria Åkerstedt , Karin Persson Waller Åse Sternesjö , Swedish University of Agricultural Sciences, 2 Uppsala, Sweden, National Veterinary Institute, Uppsala, Sweden 47. Milk Composition in Udder Quarters with Different Levels of Somatic Cell Count 1 2 3 1 1 L. Forsbäck , H. Lindmark-Månsson , A. Andrén , and K. Svennersten-Sjaunja , Swedish University of 2 Agricultural Sciences, Department of Animal Nutrition and Management, Uppsala, Sweden, Swedish 3 Dairy Association, Lund, Sweden, Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden 48. How Chlorine Bleach Compares To A Commercially Available Hydrogen Peroxide Teat Dip Charles Gradle, GEA WestfaliaSurge, Inc., Romeoville, Illinois, USA 49. Identification of an X-ray Contrast Solution for Use as an Indicator of Post-Dip Penetration into the Bovine Teat Canal 1 1 2 1 1 Kathryn Hohmann , Matt Stuessel , Kristy Brown , Kevin Torgerson , GEA WestfaliaSurge, Inc., 2 Naperville, Illinois, USA, Leon Valley Veterinary Services, LLC, Sparta, Wisconsin, USA 50. The Effect of Liner Barrel Shape on Teat End Condition Aaron K. Kochman and Chuck Laney, Lauren AgriSystems, Ltd., New Philadelphia, Ohio, USA 51. Liner Design and Teat Tissue Changes Aaron K. Kochman and Chuck Laney, Lauren AgriSystems, Ltd., New Philadelphia, Ohio, USA 52. Comparison of Real-Time PCR and Conventional Bacterial Culturing in Bovine Mastitis Testing M.T. Koskinen, Finnzymes Diagnostics, Espoo, Finland 53. Proteomics of Mastitis Causing Escherichia coli John D. Lippolis, Timothy A. Reinhardt, Marcus E. Kehrli, Jr., National Animal Disease Center, Agriculture Research Service U.S. Department of Agriculture, Ames, Iowa, USA 54. Teat Conditioning Properties of a Hydrogen Peroxide Teat Sanitizer in Automated Milking Systems 1 2 2 3 3 Xavier Goossens , Sarne De Vliegher , Lydia Bommelé , Winston Ingalls , Tom Hemling , and Mario 3 1 2 3 Lopez-Benavides , DeLaval NV, Ghent, Belgium, Ghent University, Ghent, Belgium, DeLaval Inc., Kansas City, Missouri, USA 55. Teat Conditioning Technology in a Pasture-Based System 1 2 1 1 Mario Lopez-Benavides , Rowena Harper and Winston Ingalls , DeLaval Inc., Kansas City, Missouri, 2 USA, Hamilton, New Zealand 56. Microarray Analysis of Gene Expression of Milk Leukocytes during Staphylococcus aureus Mastitis in Dairy Goats 1 2 2 3 2 3 G. Pisoni , B. Castiglioni , P. Cremonesi , A. Stella , P.J. Boettcher , S. Genini , 3 1 1 E. Giuffra , P. Moroni , Department of Veterinary Pathology, Hygiene and Public Health, Faculty of 2 Veterinary Medicine, University of Milan, Italy, Inst. of Agricultural Biology and Biotechnology, Italian 3 National Research Council, Milan, Italy, Parco Tecnologico Padano, Lodi, Italy 57. Monitoring of the Efficacy of a Bio-hygienization Treatment on the Reduction of the Microbial Load on Cubicles in an Italian Dairy Herd 1 2 3 1 1 Valerio Bronzo , Andrea Zanierato , Roberto Varano , Paolo Moroni , Department of Veterinary 2 Pathology, Hygiene and Public Health, Faculty of Veterinary Medicine, University of Milan, Italy, SOP 3 srl, Busto Arsizio, Italy, Animal Production Practitioners, Italy 58. Effect of the Selective Treatment of Clinical Mastitis Based in On-Farm Culture Results on the Risk to Receive Secondary Therapy, Days to Clinical Cure and Days Out of the Tank 1 1 1 2 3 1 Alfonso Lago , Sandra Godden , Russ Bey , Pamela Ruegg , Ken Leslie , University of Minnesota, St. 2 3 Paul, Minnesota, USA, University of Wisconsin, Madison, Wisconsin, USA, University of Guelph, Guelph, Ontario 59. Efficacy of Non Steroidal Anti-inflammatory (Meloxicam) Treatment on Milk Production, Somatic Cell Count and Culling of Dairy Cows With Clinical Mastitis 1 2 3 1 2 Scott McDougall , Mark Bryan , Richard Tiddy , Animal Health Centre, Morrinsville, New Zealand, Vet 3 South, Winton, New Zealand, Vet Resource, Te Awamutu, New Zealand 60. Efficacy of Tylosin Treatment and Factors Affecting Cure Rate of Staphylococcus aureus Subclinical mastitis Carlos Catracchia, Martín Pol, Liliana Tirante, Javier Chaves, Lactodiagnostico Sur, Olivos, Buenos Aires, Argentina