Bio 3 W 2009 Name: Question 1. Briefly define the following (6 pts) SID Catabolic metabolic pathway: Series of reactions where you start with larger, more complex, higher energy molecules and wind up with smaller, less complex, lower energy molecules with a net release of energy Anabolic metabolic pathway: Series of reactions where you start with smaller, less complex, lower energy molecules and wind up with larger, more complex, higher energy molecules with a net input of energy Question 2. For the following two diagrams relating to enzymes, fill in the blanks below each with the appropriate label. (10 pts) Figure 2A Figure 2B Figure 2A Figure 2B A+B: reactants of substrate A: substrate_____ B: active site____ C+D: products E: energy of activation w/o enzyme C: enzyme/substrate complex F: energy of activation w enzyme D: product_ In figure 2B, which of the three states (1, 2 or 3) would be least stable? 2 Question 3: Write the generalized equation for aerobic respiration/photosynthesis. In your equation you should indicate which direction describe each of the two processes, whether that process is anabolic or catabolic, and what type of energy is either produced or consumed. (6 pts) direction 1 = photosynthesis, anabolic, E = sunlight direction 2 = aerobic respiration, catabolic, ATP Question 4: Aerobic respiration 4A (5pts ) Which phase of aerobic respiration occurs in cytosol? glycolysis What is consumed during this process? glucose What is produced during this process? 2ATP, 2NADH, 2 Pyruvate 4B (5 pts) Which phase of aerobic respiration occurs in the matrix of the mitochondrion? Krebs cycle What is consumed during this process? 2 Pyruvate What is produced during this process? 6CO2, 8NADH, 2FADH2, 2ATP 4C (5 pts) Where specifically does the electron transport chain occur during aerobic respiration? Cristae of the mitochondrion What is consumed? 10NADH, 2 FADH2, oxygen What is produced? 32 to 34 ATP, H2O 4D (2 pts) What are the two electron carriers that play a role in aerobic respiration and how many ATP are each capable of producing? NADH can make 3 ATP each FADH2 can make 2 ATP each Question 5: Light (4 pts) 5A Briefly explain the relationship between the wavelength and energy with respect to the electro-magnetic spectrum. Energy is inversely proportional to wavelength 5B Why is visible light uniquely suited for the process of photosynthesis? Has the right wavelength/energy to excite electrons to higher energy levels Question 6: 6A Where would you find photosystem II and photosystem I? (1 pt) In the thylakoid membranses of the grana of the chloroplast 6B What if anything is produced and consumed as a consequence of exciting the electrons of photosystem II (2 pts) ATP and oxygen 6C What if anything is produced and consumed as a consequence of exciting the electrons of photosystem I (2 pts) NADPH Name SID 6D Where specifically does the Calvin cycle occur? (1 pt) stroma of the chloroplast 6E What is produced and consumed during the Calvin cycle? (3 pts) produced - glucose consumed – carbon dioxide, NADPH and ATP Question 7: List the four phases of a eukaryotic cell cycle and briefly describe what occurs during each phase. (8 pts) 1 gap 1 phase, cells grows as it acquires resources, DNA unduplicated and chromatin 2 S phase, DNA is duplicated, still in chromatin form 3 gap 2 phase, cell grows and acquires resources for division, DNA duplicated and in chromatin form 4 M phase, nuclear division followed by cytokinesis. DNA condenses to chromosomes, starts in duplicate form, ends in non-duplicated forms, decondenses back to chromatin at end Question 8: Briefly define the following terms (6 pts): Homologue -the partner of a chromosome that shares comparable genetic information. Sister chromatid identical copy of a chromosome joined at the centromere to its duplicate Zygote – a diploid cell resulting from the fusion of two gametes (fertilization) Haploid – the condition where a cell has only one of each homologous chromosome pair Question 9. State a significant event that occurs during each of the following stages of mitosis (4 pts) Prophase chromatin condenses to chromosome, SFN forms, nuclear envelope breaks down, SFN attaches to either side of centromere Metaphase – chromosomes aligned at equator, SFN fully formed and attached to centromeres, nuclear envelope gone Anaphase – sister chromatids pulled towards opposite poles by SFN Telophase – chromosomes decondense, SFN breaks down, nuclear envelope reforms, cytokinesis at end Question 10. State a significant event that occurs during each of the following stages of meiosis 1(4 pts) Prophase1 Synapsis, crossing over, plus any of the stuff in prophase of mitosis Metaphase1 Independent assortment of homologous pairs along equator, SFN fully formed and attached to either homologue, nuclear envelope gone Anaphase1 homologous chromosomes separtated Telophase1 chromosomes decondense, SFN breaks down, nuclear envelope may reform, cytokinesis Question 11. State a significant event that occurs during each of the following stages of meiosis 2 (4 pts) Prophase chromatin condenses to chromosome, SFN forms, nuclear envelope breaks down, SFN attaches to either side of centromere Metaphase-– chromosomes aligned at equator, SFN fully formed and attached to centromeres, nuclear envelope gone Anaphase sister chromatids pulled towards opposite poles by SFN Telophase- chromosomes decondense, SFN breaks down, nuclear envelope reforms, cytokinesis at end Question 12: Briefly describe (not list) two mechanisms during meiosis that create genetic variation. (6 pts) Synapsis & crossing over – creates unique new chromosomes consisting of a mixture of paternal and maternal genes Independent assortment of homologous pairs – creates unique combinations of maternal and paternal chromosomes Question 14: (6pts) Where does the process of transcription occur? In the nucleus What does this process produce? Three types of RNA: mRNA, rRNA, & tRNA How is it similar to DNA replication? Both transcription and DNA replication use DNA strands as templates against which new strands are formed Question 15: Briefly describe the role of each of the following during the process of translation (6 pts) mRNA carries the instructions for the sequence of amino acids for a specific polypeptide tRNA – seeks specific amino acids in cytoplasm, brings them to the ribosome and then links to the appropriate mRNA codon in order to add their amino acid to the growing polypeptid Ribosome – forms the workbench that allows tRNA and mRNA to interact in order to build the polypeptide amino acid is the raw building block with which polypeptides are assembled Question 16: What is a gene (be as specific as possible)? Where would you find it? (4 pts) A gene is a sequence of DNA that contains the instructions for the amino acid sequence of a polypeptide. It would be found in the nucleus.\