HBio, Lab 2, p. 1
Lab 2, p. 9
Names _______________________________ : _______________________________
Honors Biology
Date ________
Period _____ Score _____ Sign Off _______
Metric Measurement and Microscopy
Introduction:
What are the two main topics of this lab?
2.1 THE METRIC SYSTEM:
Define metric system:
Length
What are the five main units of length measurement studied in this lab? (You can use abbreviations)
Experimental Procedure: Length
1. see lab book
 how many cm? _______

1 cm = ________mm

1 um = ________mm
2. see lab book
 1.5 mm = ________um

1 nm = ________um

1mm = ________um = ________nm

1.5cm = ________mm

0.25 mm = ________um

5,000 nm = ________um

55 nm = ________um

1,500 um = ________mm
3. The circle is = ________mm, = ________um, = ________nm.
4. cm in a meter __________, mm in a meter __________, milli prefix = __________
6.
One bone: = ______cm = ______mm.
Temperature: YOU WILL NEED TO LOOK AT P. 475
1. Water freezes at either _______°F or _______°C
Water boils at either _______°F or _______°C
2. 68°F = _______°C
3. _____________________________________ = ________°C
_____________________________________ = ________°C
Weight: CHECK OUT A BALANCE FROM ME, SKIP TO EXPERIMENTAL PROCEDURE
Experimental Procedure: Weight
3. _______g = _______mg
4. the item is a test tube stopper and weighs ________g = _______mg
Volume:
One liter = _______ml
HBio, Lab 2, p. 2
Experimental Procedure: Volume
1. Length = _______cm; Width = _______cm; Depth = _______cm
3
= _______cm = _______ml
3. ____________________________________________________________________________
____________________________________________________________________________
total volume of the test tube _________
4. ____________________________________________________________________________
____________________________________________________________________________
the test tube stopper has a volume of _______ml
5. ____________________________________________________________________________
# drops ________ = 1ml, _______________________________________________________
2.2 MICROSCOPY
Light microscopes use light and ___________. The binocular dissecting microscopes are used to
look at entire _____________________ at a __________magnification. The compound light
microscope is used to study ________________ objects with the light coming from ___________.
Electron microscopes use a beam of _______________ focused on a photographic plate using
__________________________. A scanning electron microscope is like a ________________
scope. The Transmission electron microscope is like the ___________________ microscope giving
a 2-dimensional image. Resolution is __________________________________________________
For a compound microscope the two points must be at least ________nm apart, for an electron
microscope they can be ________nm apart.
Conclusions:

to see protein molecules….use a ______________________________

to see bacteria….use a _____________________________________

what can be viewed by both the unaided eye & the dissecting microscope….________________
2.3 USE OF THE COMPOUND MICROSCOPE
Identification of Parts
1. _______________
5. a. ________________
b. ________________
c. ________________
d. no
HBio, Lab 2, p. 3
14. no
Focusing on the Microscope –Lowest Power: Follow their directions!!!!!!
Inversion: Go to page 20 to see how to prepare a wet mount of your letter “ e”!
1.
3
2.
________________________________________________
4. ________________________________________________
Focusing the Microscope—Higher Power: Read this!!! and the Rules of the Microscope!!!!
Total Magnification:
Objective
Scanning
Ocular Lens x
Objective Lens =
Total Magnification
Low power
High power
Diameter of Field:
Low Power (10x)
2. _______mm = _______um
High Power (40x)
1. a. ________um
b. ________
c. ________
2. _______
Conclusions:
 Larger field of view….

Smaller field, but greater magnification….
Depth of Focus
Defined:
Observation: Depth of Focus
1. The slides are already prepared (up front) with three pieces of thread crossing at a center point.
2. Top ___________ Middle ___________ Bottom __________
Human Epithelial Cells
Do steps 1-5 and draw (room on next page) what you see and label 1-3: see next page for making your
drawing….wash the slide with soap, rinse and dry and return.
Onion Epidermal Cells
Same as above but label 1-2
HBio, Lab 2, p. 4
Differences
Shape (sketch and label)
Human Epithelial Cells
Onion Epidermal Cells
Cell Wall
(absent/present)
2.5 BINOCULAR DISSECTING MICROSCOPE (STEREOMICROSCOPE)
Identification of Parts
2. _______
Focusing the Binocular Dissecting Microscope (skip #4)
Laboratory Review 2
1. ________________________________________________________________________________
2. ________________________________________________________________________________
3. ________________________________________________________________________________
4. ________________________________________________________________________________
5. ________________________________________________________________________________
6. ________________________________________________________________________________
7. ________________________________________________________________________________
8. ________________________________________________________________________________
9. ________________________________________________________________________________
10. ________________________________________________________________________________
11. ________________________________________________________________________________
12. ________________________________________________________________________________
Thought Question: (incorporate the question into your answer!)
13.
14.