Due Date: Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Candidate Number: 002171-­‐ Blog resource: http://tinyurl.com/459sxqx Click4Biology: http://tinyurl.com/4dpmu73 Cite all sources using the CSE method (or ISO 690 Numerical in Word. Highlight all objective 1 command terms in yellow and complete these before class. Highlight all objective 2 and 3 command terms in green – these will be part of the discussions in class. After class, go back and review them. Complete the self-­‐assessment rubric before submitting to Moodle. Avoid printing this if possible. Learn.Genetics @ Utah (great simulations and animations): http://learn.genetics.utah.edu/ Human Genome Project: http://www.genome.gov/10001772 and http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml BioEthics Education Project: http://www.beep.ac.uk/content/index.php 1. The polymerase chain reaction (PCR) is used where DNA samples are too small to be useful. a. State the purpose of PCR in labs and investigations. • b. Identify the cellular process which PCR mimics. • c. State the role of high temperatures in PCR. • d. State the role of complementary base pairing in PCR. • e. Describe why it is called a chain reaction. • Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ 2. State two main uses of DNA profiling by electrophoresis. • • 3. State some other names for DNA profiling. • • 4. State the roles of the following components of gel electrophoresis: Restriction enzymes Gel Electric current Fluorescent DNA markers 5. Annotate the image below to outline briefly how gel electrophoresis works, including how the size of fragments affects their position on the final gel. Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ 6. Use the gel electrophoresis results below to answer these questions. In this case, a DNA sample was taken from a cigarette found at a crime scene (smoking in a no-­‐
smoking zone): a.
b.
c.
d.
7.
8.
State the process used to amplify the small amounts of DNA collected at the crime scene to an amount big enough to be used in DNA profiling. • Deduce which criminal, Rob McCarr or Nick Allott, left their dribbly cigarette-­‐end at the crime scene. Explain your answer. • Criminal: • Explanation: Draw bands to show where the standard fragments would be observed. State the role of the standard fragment. • Outline the evidence in the DNA profile that suggests Nick and Rob are related. • Describe the Human Genome Project. • Outline the goals of the HGP (http://www.beep.ac.uk/content/187.0.html ). • • • • • Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Due Date: Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Candidate Number: 002171-­‐ 9. Look at some of the HGP resources at http://www.genome.gov/25019885 a. Describe the role of E. coli in the HGP. • b. Explain how differently-­‐coloured nucleotides were used in sequencing the genes. • c. Explain why the information collected was stored in public databases. • 10. Outline how the HGP developed knowledge in the following fields: Evolutionary research • Medicine • Bioinformatics • Ethical, Legal and Social Issues (ELSI)? • 11. Genetically modified organisms (GMOs) are created using gene transfer. a. Outline gene transfer. • • • • b. Explain how the universality of the genetic code is central to gene transfer applications. • • • • c. Define transgenic organism. • Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Due Date: Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Candidate Number: 002171-­‐ d. State the role of the following in gene transfer: Restriction enzymes • E. coli plasmids • Ligase • Vector • e. Annotate the diagram below to outline the process of gene transfer: f.
Explain how gene transfer is used in industrial production of insulin. • • • • • • 12. Outline the use of gene transfer in gene therapy to treat SCID. Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ a. SCID is… b. Gene therapy can treat SCID by… 13. Give three examples of genetically modified (GM) plant crops and the effects of their new genes. New properties Advantages ‘Golden Rice’ 14. Outline how genetically modified sheep can help hemophiliacs. • • • • 15. Discuss the benefits and potential dangers of one type of genetic modification used in agriculture. Suggest why some groups are protesting GM (genetically modified) foods or crops? • • • • • 16. Define clone. Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Due Date: Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Candidate Number: 002171-­‐ • 17. Give three examples of naturally occurring clones. • • • 18. Dolly the Sheep was produced by cloning using a differentiated nucleus. a. Define differentiated nucleus. • b. Suggest why the differentiated nucleus taken from an udder cell. • c. Suggest one reason why Dolly died younger than normal, but of age-­‐related illnesses. • d. Outline the method used to clone Dolly the Sheep. • • • • • Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ 19. Distinguish between reproductive cloning and therapeutic cloning. • Reproductive = • Therapeutic = 20. State some of the medical applications and benefits of therapeutic cloning. • • 21. Discuss the ethical considerations of therapeutic cloning in humans. Advantages: Disadvantages: 22. Suggest why stem-­‐cell reprogramming (IPS cells) might mitigate the negative opinions of human therapeutic cloning. Source: http://www.nature.com/nature/journal/v451/n7180/full/451858a.html a. Define iPS stem cells. • b. Outline the process for reprogramming the cells. • c. Explain the benefits of using iPS cells in medicine and research. • Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ Works Cited 1. Allott, Andrew. IB Study Guide: Biology for the IB Diploma. s.l. : Oxford University Press, 2007. 978-­‐0-­‐
19-­‐915143-­‐1. 2. Mindorff, D and Allott, A. Biology Course Companion. Oxford : Oxford University Press, 2007. 978-­‐
099151240. 3. Clegg, CJ. Biology for the IB Diploma. London : Hodder Murray, 2007. 978-­‐0340926529. 4. Campbell N., Reece J., Taylor M., Simon. E. Biology Concepts and Connections. San Fransisco : Pearson Benjamin Cummings, 2006. 0-­‐8053-­‐7160-­‐5. 5. Taylor, Stephen. Science Video Resources. [Online] Wordpress, 2010. http://sciencevideos.wordpress.com. 6. Burrell, John. Click4Biology. [Online] 2010. http://click4biology.info/. 7. IBO. Biology Subject Guide. [Online] 2007. http://xmltwo.ibo.org/publications/migrated/production-­‐
app2.ibo.org/publication/7/part/2/chapter/1.html. Self Assessment: Essential Biology Criterion Presentation & Organisation Academic Honesty Objective 1 understanding Objective 2 understanding Objective3 understanding Logic, notation, mathematical working Further research Assessment Complete (2) Partially complete (1) Self MrT NA Complete and neat. All command terms highlighted, tables and diagrams well presented. Sources cited using the CSE (ISO 690 numerical) method, with Works Cited section complete and correct. All answers for the following command terms Most answers for the following command terms correct: correct: Define Draw Label List Measure State Most answers for the following command terms All answers for the following command terms correct: correct: Annotate Apply Calculate Describe Distinguish Estimate Identify Outline Most answers for the following command terms All answers for the following command terms correct: correct: Analyse Comment Compare Construct Deduce Derive Design Determine Discuss Evaluate Explain Predict Show Solve Sketch Suggest Answers are presented in a logical and concise manner. SI units used most times, with correct NA unit symbols and definitions of terms. All mathematical working shown. Evidence is apparent of research and reading beyond the textbook and presentations to find correct answers to challenging questions. If any NA questions are unanswered, this criterion scores zero. NA Stephen Taylor Bandung International School http://sciencevideos.wordpress.com Essential Biology 4.4 Genetic Engineering & Biotechnology Student Name: Due Date: Candidate Number: 002171-­‐ Total (max 10): Stephen Taylor Bandung International School http://sciencevideos.wordpress.com