Bio 126: Energy Flow in Biological Systems Revised 4/28/06 CO2 Lab Report Stylesheet Due Date: by 5:00 pm on the day after your lab, May 17-19 Scientific Paper Format: Your report should be typed or computerprinted, and stapled. All pages should be numbered (except the Title Page). The different sections of the lab report should each be labeled ("Abstract," "Introduction," etc.). Double-space and leave approximately 1-inch margins all around, so that we have room to write comments. Contents: - Full lab report - You must cite at least one primary literature paper (see Literature Cited section below). Note that a web page is not a primary literature paper. For general information on the format of a scientific paper, follow the format given in Chapter 4 of “A Student Handbook for Writing in Biology” by Knisely – you should have already read this chapter for the Alkaline Phosphatase paper you wrote a few weeks ago. You should also read through chapters 3, 5, and 6. This stylesheet highlights what we think is important in writing scientific papers for this course. Where there is a conflict between Knisely (2005) and this stylesheet, you should follow this stylesheet. Do not hesitate to seek the advice of your instructor, TA, or the writing tutors at The Write Place. Introduction: Scientists use several types of papers to communicate with one another in “the literature” (i.e., published journals and books). The major mode of written communication is called a primary paper. It describes the research performed by the author(s) – how she/he did the study, what the results were, and what the author thinks the results mean. Since articles in scientific journals are the main method of written communication among scientists, we feel that it is important that students in the introductory biology classes gain skills in writing papers in this format. This is the type of paper you will be writing for the CO2 lab. Another type of paper, which you will find very useful for getting an overview of an area of research, is called a review paper. Review papers are not primary literature; they usually are an attempt to put together a cohesive story based on many primary papers. Review papers are often a good place to find primary papers you are interested in, since the authors try to incorporate all of the literature relevant to a given topic. Senior biology students who are working on their comps are writing papers which are much like review papers. For your written assignment, use the class data to calculate statistics for your experiment (as described in the Bio 126 lab manual). You should use the data from the entire lab section – these data are on the Excel spreadsheet in the Collab folder for your lab section. We encourage you to analyze your results and discuss their implications with your lab partners. However, when you get around to writing your paper, you may not even discuss an outline of what you will write with another student in the class. Your paper should be a solo effort. 1 Bio 126 – CO2 Report Stylesheet experimental question, the methods you used, your results and your interpretation of the results. In short, the abstract should summarize all other sections of the paper – someone should be able to read just the abstract and understand what you did, what you found, and what you think it means. Meeting with your TA: You are expected to meet with your TA during the week after the CO2 lab to discuss your lab report. Your TA will bring a sign-up sheet to the CO2 lab during week 6 so you can sign up for a slot. To this meeting, you should bring a draft of your lab report. This meeting will provide an opportunity for you to ask questions about the format of the lab report, as well as data analysis and interpretation issues. To receive full credit for your rough draft, there should be text in all sections of the report and at least two figures. 3) Introduction Here you should introduce the experiment. First you should develop a context for the experiment, introducing the issues that you will address in the experiment. For example, in the CO2 experiment, it would be good to say a few words about rising levels of atmospheric CO2 and introduce the general responses that plants show (i.e., what other researchers have found in the past). In addition, you should give a clear statement of your experimental question and a rationale for asking the question (i.e., why the experimental question is interesting and/or important). It would be good, too, to include a brief summary of how you addressed the experimental question. Audience for the Report: Knisely pp. 33-34 & 71 As with the Alkaline Phosphatase paper, the reader of your paper is a person with an introductory biology-level of understanding of biology. Your reader, however, knows nothing about your experiment – hypotheses, methods, etc. For example, when you write your report for the CO2 experiment, your descriptions should not assume any prior understanding of how you set up your experiment. Your reader, however, will know the basics of photosynthesis and the Calvin cycle. 4) Materials and Methods As in the Alkaline Phosphatase report, you should present the experimental methods clearly and concisely. In this section, state the species used (and variety of tomato), how the experiment was set up, how data were collected, and how the data were analyzed, including any statistical tests that were performed. There should be just enough detail so that someone else could repeat this experiment if they read this section. You may cite the laboratory manual to refer your reader to a detailed description of the statistical tests (don't provide any mathematical formulas). Since you will be reporting on what you have done, use past tense. It is important to report the methods for the entire experiment, not just the data you personally collected. Though it may seem artificial for this experiment, report the Components of the Lab Report: 1) Title Page This should include a concise and informative title for your report, your name, and your lab section. Ideally the title should provide information about the experimental question and results. The rest of the report should begin on the next page. 2) Abstract This section should be brief (not more than about 5% of the total length of your paper) and comprise a single paragraph. It should concisely summarize the rationale and purpose of your experiment, statement of the 2 Bio 126 – CO2 Report Stylesheet implications of the results; save that for the Discussion section that follows. You will be marked down if the text does nothing more than tell the reader to look at a table or figure, as in "The results are summarized in Table 2." Check Knisely's book (pp. 38-41) for help with referring to your figures. experiment as if you conducted it yourself. You should not confuse your reader by mentioning the members of your lab group or partner, the division of labor while conducting the experiment, etc., unless you think the information is of vital importance. Also, do not present a recipe description of the methods, such as " first I did this, then I did this, then I did that, ...". Your goal is to provide a clear, concise description of the experiment – a chronological list of events is usually not necessary. You should make all of your figures yourself; it is not acceptable just to make copies of the same figure for all of the students in a group. Also, the figures and tables may be placed on separate pages at the end of the report or inserted between paragraphs in the text. Each figure and table should have an explanatory caption (also called a legend) that includes: (1) the figure or table number, (2) a descriptive title for your figure or table, (3) a description of where the data came from and (4) a description of the important features of the data in the figure/table (e.g., how many trials, what error bars correspond to). A table caption goes above the table, while a figure caption goes below the figure. Following the figure or table number, the title is usually an incomplete sentence with only the first word capitalized. Do not put an additional title at the top of your graph. Study the sample graphs and tables in Knisely carefully to get a sense of proper format. 5) Results The Results section contains a text account that describes all of the important experimental results, including the major trends, patterns, and differences in the data. In addition, for this lab you should have a separate bar graph with error bars for each measurement that shows statistically significant differences. Though the figures present results, you must verbally describe all of the important trends and patterns in your data. Also, it is important to describe the results of the statistical tests (i.e., t-tests), but keep in mind that statistics are only a tool to see if patterns or differences are real – focus on the results and the biology, not on the statistical results. See the example text below: “Plants grown in an elevated CO2 environment had significantly more leaves than plants grown at ambient levels of CO2 (t=3.45, p<0.01, df=8) (Figure 1)." 6) Discussion Here you tie everything together. It is often good to start the section by summarizing your basic findings and evaluating the hypotheses you set out to test in this experiment. You need to make clear, logical connections between experimental question(s), results, and conclusions. For the CO2 experiment, you should attempt to explain your results. In particular, what might be the physiological basis for your results? In addition to using your experimental results, you should bring in other relevant information to answer the experimental You should note three things about this sentence. First, the main trend in the data is described in the text. Second, the statistical results are in parentheses, since they just provide support for the trend. And third, the reader is referred to a figure showing these trends on a bar graph. While you must describe your results in this section, be careful not to interpret the overall 3 Bio 126 – CO2 Report Stylesheet question. This information could come either from the laboratory handout or other references (e.g. your class textbook or a journal article). For example, in the CO2 experiment, do data from other studies support your results? When interpreting your data, keep in mind the limits of your data and reference material. Finally, you should discuss the broader implications of your results. For example, you may want to discuss why your findings may be important, and how they might have implications outside your study. What will happen to plants in a larger area (for example in agriculture or in natural communities) if atmospheric levels of CO2 continue rising? Also, might your results suggest other experiments that could be used to further answer your experimental question? Citations are parenthetical, and consist of the author's name followed by the year published. Parenthetical citations at the end of a sentence always go before the period for that sentence. You must cite each kind of information individually: do not put one citation at the end of a paragraph in the hopes that this will cover the whole paragraph. Make it clear exactly what you are citing; if you are using information from an outside source in the first half of a sentence, don't leave the parenthetical citation until the end of the sentence. When you cite the lab manual in the text of your paper, follow the statement with ‘(Hougen-Eitzman, 2006).’ If you cite an idea or fact from a textbook or journal article, follow the statement with ‘(lastname, year),’ such as (Raven et al., 2005) or (Watson and Crick, 1953). If you state some information given to you by your instructor, follow the statement with ‘(First Initial. Lastname, personal communication),’ such as (C. Darwin, personal communication). Students often feel compelled to consider any major problems with their experiment. However, mistakes or errors in an experiment are only relevant if they could have altered the conclusions or interpretation of the results. Problems that would not have affected your conclusions should not be discussed. You should have a Literature Cited section for the paper. Since you should cite at least one primary literature article, at minimum there will be one complete citation. Other citations could include your class textbook, the lab manual, articles referred to in the lab manual, or even articles you find yourself on the Web of Science. Remember that you need to cite any information that you did not create yourself. The citation(s) should be listed in the Literature Cited section of your paper in alphabetical order by author. Follow the format given in Chapter 4 of Knisely (pages 58-65). If you cite a web page, please include the date on which you accessed the page, as shown below: 7) Literature Cited You are not required to do any an independent literature search for this report. However, you should cite at least one primary literature paper (for example, you could choose one of the papers cited in the lab manual, which are available on the Bio 126 lab web page). The most likely location for your citation is the introduction and/or discussion sections. When you write your lab report, you must give the source for any idea or data that is not your own (either from your own head or your own experiment). We will be using the Name-Year System (see Knisely, pp. 58-65), which is common to many scientific journals. [Anonymous]. 2006. CO2 Lab Report Stylesheet. Biology 126 Lab Web Page. <https://www.carleton.edu/departments/BIOL/courses/f acstaffstu_only/bio126/2006%20lab/CO2_report_Style sheet.pdf> Accessed 2006 April 28. 4 Bio 126 – CO2 Report Stylesheet Revising your Report After you write your lab report, we recommend that you read through your draft carefully and revise it. The helpful staff at The Write Place is willing to read your draft and make comments. You should also feel free to ask advice from your lab instructor or TA. Rarely can someone write a first draft that needs no subsequent changes or improvements. Chapter 5 in Knisely (2005) contains good advice on how to read and revise your report. You should also carefully proofread your paper to find and correct all spelling and grammatical errors. 5