BiologyHonorsThesisGuidelines Purpose:toprepareBiologyHonorsstudentstowriteuptheirresearchprojectintheformofascholarly SeniorHonorsThesis. Thisdocumentprovidesguidelinesforwhatisoftenthemostintimidatingpartoftheresearchproject: writinguptheHonorsThesis.AftercompletingyourHonorsThesisproposalandcollectingandanalyzing yourdata,youwillpresentyourworkininoralandwrittenformats.Goodworktakestime,sostartingthe writingprocessearlyandallowingsufficienttimetorevisearekeytowritingahighqualitythesis(and loweringyourstress).Thegoodnewsisthatyoualreadyhavestartedthisprocess.InwritingyourThesis Proposal,youexploredyourtopicintheprimaryliterature,andrationalizedyourstudy.Sincethen,you have,nodoubt,collectedandreadadditionalresearcharticlesandbecomemoreknowledgeableaboutyour topic.Still,theThesisProposalisagoodstartingpointforbeginningtowriteyourThesisandyoumaywish tosimplyaddinformationtotheThesisProposalwhilewritingyourHonorsThesis. GeneralFormat:YourHonorsThesiswillfollowtheconventionalformatforascientificresearchpaper, includingthefollowingsections:Abstract,Introduction,MaterialsandMethods,Results,Discussion,and LiteratureCited.TheHonorsThesisshouldbewrittenaccordingtoconventionsofscientificwritingusing clear,concisesentencestructure,well-developedparagraphswithstrongtopicsentences,andalogical progressionofideas.Forin-depthdetailsonwritinginBiology,itmaybeusefultorefertothematerialsused inyourWritingIntensiveBiologyclass(e.g.,BIO306WorBIO311W),including“AStudentHandbookfor WritinginBiology”byKarinKnisely(2013).WhiletherearenotspecificpagelimitsfortheHonorsThesis,itis likelythatatleast20pages(12-pointfont,double-spaced,notcountingfiguresorworkscited)willbeneeded toprovidesufficientbackgroundinformationandtodocument,analyze,andcontextualizeexperimental results. Timeline:Goodwritingtakestimeandmultipledrafts.Waitingtowriteupyourthesisuntiltheendofthe semesterislikelytoresultinanunacceptablypoor-qualityproduct.BeforestartingtowritetheHonors Thesis,itisagoodideatoformulateaplanandtimetable.Duringthisplanningprocess,makesuretoleave enoughtimeformultiplerevisions(allowingyourresearchmentorabouttwoweekstocommentoneach draft).DraftsthatarenotuptostandardsmayberejectedbytheBiologyHonorsAdvisor,andevendrafts thatarewelldonearelikelytorequireatleastoneadditionalroundofrevisionsbasedoncommentsfrom theHonorsAdvisor.Followingthetimelinebelowwillimprovethequalityofyourwriting. Year Date 1-2 MeetwithBIOHonorsAdvisor,begintoexploreresearchopportunities 2 Identifyaresearchadvisor;begindiscussingpotentialprojects 3 Sept-Nov MeetwithBIOHonorsadvisortodiscussThesisProposal,prepareThesis Proposal,submitThesisProposaltoresearchadvisor,revise 3 January31 ThesisProposalduetoBIOHonorsadvisor 3-4 Conductexperimentsandcollectdata 4 January UndergraduateSymposiumAbstractdue 4 January Completedataanalysis 4 Jan-March PreparedraftofHonorsThesisandUndergraduateSymposium presentation 4 EarlyMarch SubmitdraftofHonorsThesistoresearchadvisor,revise 4 LateMarch UndergraduateSymposium(oralorposterpresentation) 4 April1 HonorsThesisduetoBIOHonorsAdvisor 4 Mid-LateApril ReviseHonorsThesisbasedoncommentsfromBIOHonorsAdvisor 4 LateApril AfterapprovalbyBIOHonorsAdvisor,submitfinalrevisedHonorsThesis toHonorsCollege 1 GuidelinesandTips: • • Title.Thetitleshouldspecifytheexperimentalsystemusedaswellaskeyvariablesanalyzedand/or significantdiscoveries.Itshouldbespecific,informative,andbrief. Abstract.Theabstractisaone-paragraphcondensationoftheentirework.Theabstractshouldbea self-containedunitcapableofbeingunderstoodwithoutthebenefitofthetext.Itshouldcontain fiveelements,inthisorder:1)anintroductorysentencehighlightingtheimportanceofthetopic;2) thepurposeofthestudyandthehypothesestested;3)abriefstatementofthegeneralapproach takentotestthehypotheses(themethodsused);4)astatementofwhatwasfound(theresults);5) abriefstatementofconclusion.Themajorportionofyourabstractshouldbepresentationof results.Limittheabstractto250words. • Introduction.Thissectionisdesignedtodrawthereaderinandtocontextualizeandrationalizethe study.TheIntroductionprovidescontextbyexplainingwhyatopicisimportantorrelevantandby describingwhatisknownandwhatgapsremaininourknowledgeofthetopic.Youshouldstructure theIntroductionasafunnelconsistingofthefollowingthreeelements(eachofwhichmayrequire multipleparagraphs): o o Anexplanationofthegeneralbiologicalissueanditssignificance Areviewthestateofcurrentknowledgerelatingtoyourtopic(includingsignificant unansweredquestionsorgapsinunderstanding) Adescriptionofthespecificresearchquestionyourstudyaddresses o Tomakeyourcase,youwillneedtoreferencetheprimaryliterature.Itisoftenusefultocitereview articlesrelatingtoyourtopicintheopeningparagraphs(andreadingreviewarticleswillhelpyouget ahandleonthecurrentstateofthefield).InthefinalparagraphofyourIntroduction,includethe goal/purposeofthestudy,thehypothesisandpredictions,andthegeneralapproachusedtotestthe hypothesis.Besuretoincludeabiologicalmechanism(theexplanationwhy)withinthehypothesis. Theuseofoneormorefigures/modelswithintheIntroductionisrecommendedtoillustrate importantconceptsrelatingtoyourhypothesisand/orexperimentaldesignandlogic.Theuseof topicalsubheadingswithintheIntroductionisrecommendedasawaytoimproveorganizational clarityofthissectionoftheHonorsThesis. • • MaterialsandMethods.ThissectioncanoftenprovidesagreatstartingpointforwritingaThesisor researchpaper.TheMaterialsandMethodssectionshoulddescribeyourresearchapproach, includingspecificreferencesforexperimentaldesign,laboratorytechniques,specificreagent formulations,analysesorprocessing,andstatisticalanalyses,asappropriate.Whiletheinformation inthissectionoftheThesisshouldnotbethesameasadetailedprotocol,thedescriptionsshould providesufficientdetailtoenableanindividual“familiarwiththeart”torepeatyourexperiments. Theuseofcitationstorelatedstudiesemployingsimilartechniqueslendscredibilitytoyour approach,thoughyoumayalsoincludenovelapproaches.Ifthereareseveralpartstoyourstudy, theuseofsubheadingscorrespondingtoeachportionisrecommended.Ifyourprojectisafield study,thefirstsub-sectionshoulddescribethestudysite.Ifyouareworkingonaparticular organism,youmightbeginwithasub-sectionrelatedtoitsnaturalhistoryoritsuseasamodel organism.Considerusingvisualaidstoillustrateexperimentaldesignorprocedures.Inorderto learnaboutfield-specificconventionsfordescribingparticularanalyses(orotheraspectsrelatedto theMaterialsandMethods),seekingexamplesfrompublishedworksinthefieldisrecommended. Results.Thefunctionsofthissectionareto(1)summarizegeneraltrendsinthedatawithout comment,bias,orinterpretation,and(2)reporttheresultsofstatisticalanalysestestingyour hypotheses.Conclusionsaboutwhetheryourresultssupportorrefuteyourhypothesesshouldbe 2 savedfortheDiscussion.LikeintheMethodssection,youshouldorganizetheResultssectionwith sub-headings,andthesesub-headingsshouldmirrortheorderinwhichtheyweredescribedinthe Methodssection.Describeyourresultsinwords,referringtotablesandfiguresparentheticallyto supportyourstatements.Itisimportanttorememberthattablesandfiguresdonotsubstitutefora writtensummaryofthefindings.Inshort,thetextshouldbeabletobeunderstoodbysomeone whohasnotseenyourfiguresandtables,whilefacilitatinginterpretationofthefiguresandtables. • Results–useoffiguresandtables.Experimentaldatashouldbepresentedusingfiguresortables,as appropriate.Figures(graphs,maps,anddiagrams)andtablesareindependentunitsofinformation. Theyeachpossessdescriptiveheadingsthatwillallowthemtobeunderstoodbysomeonewhohas notyetreadthepaper.ThesecaptionsappearBELOWfiguresandABOVEtables.Graphs,maps,and diagramsarelabeledas"figures"(Fig.1,Fig.2,etc.),andtablesarelabeledas"tables"(Table1, Table2,etc.).Datashouldbedisplayedinonlyoneformat;i.e.,youshouldchoosewhetherafigure oratableisthebestwaytopresentthedata.Ingeneral,avisualfigureispreferred,butincases whereafigurebecomestoocomplicatedforeasyinterpretation,thereistoomuchdata,and/orno trendisapparent,atablemaybebetter. • TipsforwritingtheResultssection: o Choosedatapresentations(e.g.,figures,tables,gelimages,etc.)thatarebestsuitedto displayandsummarizethedataandillustrateanyspecifictrends(ifpresent).Besureto carefullyandunambiguouslydescribealldatapresentationsinthecaptiontofacilitatetheir interpretation. o Statementsaboutresultsshouldbebackedupwithstatisticaltests,wheneverpossible.For example,“Seedsetofplantsgrowninthepresenceofpollinatorswastwotimesgreater thantheseedsetofplantsfromexclosureplots(t=3.8,df=20,p<0.05).” o Whenusingstatisticaltests,emphasizethebiologicalresult,notthestatisticalresult. ! Noteffective:“Thestudent’st-testwith20degreesoffreedomshoweda statisticallysignificantdifferencebetween…” ! Hint:Usethedependentvariable(whatyoumeasuredorcalculated)asthesubject ofthesentence. o Statehowmuchofaneffecttheindependentvariablehadonthedependentvariable (=effectsize),inadditiontomentioningtherewasasignificantdifference. o Besuretopresentthegeneraltrends,nottheexactdatavaluesinthetextoftheResults section.Thesevaluesareprovidedinyourtableorfigure. o Besuretoincludetextdescription(s)ofcontroltrial(s),andcompare/contrastresultsof thesewithtrialsinvolvingexperimentalvariables.Controlresultsalsoshouldbeincludedin figures,tables,etc. • Discussion.Thefunctionsofthissectionareto(1)evaluatethemeaningofyourresultsintermsof youroriginalhypothesis,(2)offerinterpretationandexplanationsforyourresults(whetherthey supportyourhypothesisornot),and(3)compareandcontrastyourresultstothosefromrelated studies.Userelevantliteraturetohelpcomeupwithexplanations.Itisoftenusefultoorganizethe Discussionsectionasa“reversefunnel,”startingwithyourstudyandmovingtothebiggerbiological issuethatyourstudyaddresses.AsintheIntroduction,theuseofsub-headingsintheDiscussion sectionisrecommendedimproveclarityandorganization.Withineachsection,topicsentences shouldbeusedtostructuretheflowoftheDiscussionsection.TheDiscussionsectionshould integrateyourdatawiththerelevantliteraturetoprovideadetailedinterpretationoftheresearch dataandresearchquestion(s). BegintheDiscussionwithaparagraphthathighlightstheresultsofyourexperimentsandwhether yourresultssupportorrefuteyourhypothesis,andoffersabiologicalexplanationforyourresults(if youhavemorethanonehypothesis,youmightdothisforeachhypothesis-intheordertheywere 3 presentedinyourIntroduction).Youcanalsocompareyourresultstothosefromotherstudies(do theyagree?disagree?andofferpossibleexplanationswhyorwhynot).Rememberthatyourjobis toanalyzeyourownresults.Youshouldnotdedicatewholeparagraphstodescribingother researcher'sstudies;theirfindingsshouldbeintegratedwithyoursinordertoformulatecausal explanationsofyourresults. TheDiscussionsectionalsocanaddressanyproblemsthataroseandwhereyourstudyleadsus, however,youwanttotakeapositiveapproachtodiscussing“problems.”Onewaytodothisisto frameaproblemasalimitationofyourstudyandthenproposewhatshouldbedonetoaddressthis limitation.Whenproposingfuturestudies,describespecificallywhattypesofadditionalstudies mightbeusefulandwhatsuchstudieswouldtellus.Speculationisvaluableforstimulatingfurther thought. • BesuretoendyourDiscussionwithashortConclusionparagraphthatsummarizesyourmajor findingsandfitsthemintoalargerbiologicalcontext.Whatarethemostimportanttake-home messagesfromyourstudyforthereader? Literaturecited.Chooseapremierjournalinyourfieldandconsistentlyapplythebibliographic formattingofthatJournalwithinyourHonorsThesis.Youshouldaimforatleast20references, includingseveralreviewarticles.TheZoterobibliographicmanagementtool(http://www.zotero.org) shouldbeusedtoformatthein-textcitationsandbibliographyaccordingtotheconventionsofthe journalyouselect. 4