BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
J. Patrick Card
Professor of Neuroscience
eRA COMMONS USER NAME (credential, e.g., agency login)
jpcard
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and
residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
Ashland University, Ashland, OH
Wright State University, Dayton, OH
Wayne State University, Detroit, MI
UC San Diego & SUNY, Stony Brook
B.S.
M.S.
Ph.D.
Post-doc
06/1968
04/1975
04/1979
06/1981
Comprehensive Science
Biology
Neuroanatomy
Neuroscience
A. Personal Statement
My research program focuses upon the synaptic organization and function of neural circuitry influential in
the integration of sensory input for adaptive changes in behavior and physiology. Toward this end my
laboratory has maintained a longstanding collaboration with the laboratory of Dr. Lynn Enquist to develop
viral transneuronal tracing technology and apply it in systems-based analyses of functionally defined
circuits. We have employed this technology to define the synaptology of phenotypically and functionally
defined circuits integral to a variety of systems and to characterize the plastic reorganization of behaviorally
defined circuits in the developing and mature brain. A particular focus of the laboratory has been to define
brainstem and forebrain circuits that integrate primary and processed sensory information to generate
adaptive changes in physiology that support motivated behaviors. Through these investigations we have
developed considerable expertise in polysynaptic pathways through which all regions of the neuraxis
communicate and thereby generated a comprehensive expertise in systems organization of the CNS.
B. Positions and Honors
1973-1974
1982-1985
1985-1990
1990-1992
1992-2008
2002-2005
2004200620081978-1979
1978-1979
1989-1990
1993
1994
1995
1995
Faculty Associate, Department of Anatomy, Wright State University, Dayton, OH.
Research Assistant Professor, Dept. of Neurology, SUNY Stony Brook, Stony Brook, NY.
Principal Scientist, Central Research & Development Dept., DuPont Co., Wilmington, DE.
Senior Research Scientist, DuPont Merck Pharmaceutical Co., Wilmington, DE.
Associate Professor, Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA.
Co-Director, CNUP Graduate Program, University of Pittsburgh, Pittsburgh, PA
Co-Director, NIH/NCRR Center for Neuroanatomy with Neurotropic Viruses
Director, CNUP Graduate Admissions Committee, University of Pittsburgh,
Professor, Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA
Graduate Professional Scholarship, Wayne State University, Detroit, MI.
Charles B. DeVlieg Fellowship, Wayne State University, Detroit, MI.
Member, Site Visit Review Panel; NSF Science and Technology Centers.
NIH; Visual Sciences C Study Section, Ad hoc member.
NIH; Visual Sciences C Study Section, Ad hoc member.
NSF; Special Review Panel; Research Experiences for Undergraduates.
National Institute of Mental Health Special Emphasis Panel RFA
MH-95-003 “Role of the Blood-Brain-Barrier in HIV Neuropathogenesis”
1996-1998 Co-President, Pittsburgh Chapter, Society for Neuroscience
1997
NIMH; Mental Health AIDS and Immunology Review Committee, Ad hoc member.
1997
NIMH; Mental Health Special Emphasis Panel RFA MH-97-001
“Innovative Approaches for Microscopic Tract-Tracing”
1998-2001 Society for Neuroscience; Program Committee.
1999Science & Mathematics Advisory Board, Ashland University, Ashland, OH
1999
NIH; RFA No. NIH-NDCD-92-05 Study Section, Ad hoc Member
“Transport of Substances in Olfactory System”
C. Peer-reviewed Publications (Selected from 135)
Five most relevant to this application
Wojaczynski, G. J., Engel, E. A., Steren, K. E., Enquist, L. W., Card, J. P. The neuroinvasive profiles of
H129 (Herpes Simplex Virus Type 1) recombinants with putative anterograde-only transneuronal
spread properties. Brain Structure & Function, DOI 10.1007/s00429-014-0733-9
Card, J. P., Kobiler, O., McCambridge, J., Ebdlahad, S., Shan, Z., Raizada, M. K., Enquist, L. W.
Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus.
PNAS USA, 108: 3377-3382, 2011.
Card, J. P., Kobiler, O., Ludmir, E. B., Desai, V., Sved, A. F., Enquist, L. W. A dual infection pseudorabies
virus conditional reporter approach to identify projections to collateralized neurons in complex neural
circuits. PLOS On 6:1-12, 2011.
Card, J.P., P. Levitt, M. Gluhovsky, L. Rinaman. Early experience modifies the postnatal assembly of
autonomic emotional motor circuits in rats. Journal of Neuroscience 25: 9102-9111, 2005.
Cano, G., J.P. Card, A.F. Sved. Dual viral transneuronal tracing of central autonomic circuits involved in
the innervation of the two kidneys in rat. Journal of Comparative Neurology 471: 462-481, 2004.
Ten additional publications relevant/important to the field.
Card, J.P., L. Rinaman, J.S. Schwaber, R.R. Miselis, M.E. Whealy, A.K. Robbins, L.W. Enquist.
Neurotropic properties of PRV: Uptake and transneuronal passage in the rat central nervous system.
Journal of Neuroscience 10: 1974-1994, 1990.
Card, J.P., M.E. Whealy, A.K. Robbins, R.Y. Moore, L.W. Enquist. Two alpha herpesvirus strains are
transported differentially in the rodent visual system. Neuron 6: 957-969, 1991.
Rinaman, L., J.P. Card, L.W. Enquist. Spatio-temporal response of astrocytes, microglia and macrophages
to pseudorabies virus infection of central visual pathways in rat. Journal of Neuroscience 13: 685-702,
1993.
Card, J.P., L.M. Rinaman, R.B. Lynn, B.-H. Lee, R.P. Meade, R.R. Miselis, L.W. Enquist. Pseudorabies
virus infection of the rat central nervous system: ultrastructural characterization of viral replication,
transport and pathogenesis. Journal of Neuroscience 13: 2515-2539, 1993.
Card, J.P., J.R. Dubin, M.E. Whealy, L.W. Enquist Influence of infectious dose upon productive replication
and transynaptic passage of PRV in the rat central nervous system. Journal of NeuroVirology 1: 349358, 1995.
O’Donnell, P., A. Lavin, L.W. Enquist, A.A. Grace, J.P. Card. Interconnected parallel circuits between rat
nucleus accumbens and thalamus revealed by retrograde transynaptic transport of pseudorabies virus.
Journal of Neuroscience 15: 2143-2167, 1997.
Card, J.P., L.W. Enquist, R.Y. Moore. Neuroinvasiveness of pseudorabies virus injected intracerebrally is
dependent upon viral concentration and terminal field density. Journal of Comparative Neurolology
407: 438-452, 1999.
Rinaman L., P. Levitt, J.P. Card. Progressive postnatal assembly of limbic-autonomic circuits revealed by
central transneuronal transport of pseudorabies virus. Journal of Neuroscience 20: 2731-2741, 2000.
Card, J.P., D.J. Santone, M.Y. Gluhovsky, P.D. Adelson. Plastic reorganization of hippocampal circuitry in
experimental traumatic brain injury in the immature rat. Journal of Neurotrauma 22: 989-1002, 2005.
Card, J.P., J.C. Sved, B. Craig, M. Raizada, J. Vazquez, A.F. Sved. Efferent projections of rat
rostroventrolateral medulla C1 catecholamine neurons: Implications for the central control of
cardiovascular regulation. Journal of Comparative Neurology 499: 840-859, 2006.
D. Research Support
Active:
5 P50 RR 018604-05 (Card/Strick) 6/1/2009 – 5/31/2015
NIH
$75,817 (annual direct)
2.60 AY – no salary
1.00 Summer months
3.60 months (30%) TOTAL
National Center for Experimental Neuroanatomy with Neurotropic Viruses
The goal of this project is to support a state of the art National Resource Center that serves as a technical and
intellectual resource for those aspiring to use viral transneuronal tracing.
Recently Completed:
1 RO1 HL093134-01 (Card)
NIH
4/1/2009 – 3/31/2014
2.60 AY – no salary
$267,304 (annual direct) 1.00 Summer months
3.60 months (30%) TOTAL
C1, Rostroventrolateral Medulla and the Central integration of Cardiovascular Regulation
The goal of this project is to test the hypothesis that C1 catecholamine neurons in the rostroventrolateral medulla
coordinate neuronal activity within the central cardiovascular network controlling blood pressure.
NS IOS-0918867 (Sved/Card)
8/1/09 – 7/31/14
0.20 AY – no salary
$12,673. (annual direct) 1.00 Summer months
1.20 months (10%) TOTAL
Viral Microdissection of Central Circuitry Integrating Autonomic Function
This project uses transneuronal transport of pseudorabies virus (PRV) genetically engineered to allow us to
simultaneously trace multiple pathways innervating several distinct target tissues in rats.
RC1NS068414-01 (Enquist/Card) 9/30/09 – 8/31/12
0.20 AY – no salary
NIH Challenge Grant
$63,229 (annual direct) 1.00 Summer months
PRINCETON UNIVERSITY Subcontract
1.20 months (10%) TOTAL
Viral Brainbow: Tracing Brain Circuits with Connection Order Specificity
The goal of this project is to develop novel technology to study the synaptic organization of functionally defined
circuits in the nervous system.
5 P50 RR 018604-05 (Card/Strick) 6/1/2004 – 5/31/2009
NIH
$81,334
0.60 AY – no salary
3.00 Summer months
3.60 months (30%) TOTAL
National Center for Experimental Neuroanatomy with Neurotropic Viruses
The goal of this project is to establish a state of the art National Resource Center that will serve as a
technical and intellectual resource for those aspiring to use viral transneuronal tracing.