Laboratory: Timing of the Cell Cycle Name ________________________ Introduction: How long do onion root tip cells spend in each phase of the cell cycle? In this computer lab you will identify the phases of the cell cycle. You will also determine the time an onion cell spends in each part of the cell cycle. Webpage: http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/01.html Procedure: 1. 2. 3. 4. 5. 6. Open the webpage Examine the picture of the cell in mitosis and identify the phase that it belongs in by clicking on the name of the phase. Continue to do this until you have identified 36 cells and record the number of cells in Row A Calculate the fraction of cells in each phase by dividing the number of cells in that phase by the total number of cells you counted (36). Record your fraction in Row B. Express your answer as a decimal in Row C. You may need to round to the nearest hundredth. For example, .576 rounds to .58 Calculate the number of degrees in a circle graph for each phase by multiplying the time, expressed as a decimal (Row C) by 360° (the number of degrees in a circle.) Record your degree value in Row D. You may need to round to the nearest degree. For example, 87.56 degrees will round to 88 degrees. Make a circle graph showing the amount of time, in degrees, a cell spends in each phase of the cell cycle. Color each portion of the circle a different color. Label each portion with the phase, percent value, and the degrees. Make a key/legend that indicates which phase each color represents. Data Table: Interphase Prophase Metaphase Anaphase Telophase Row A Total # of cells in each phase Row B Fraction of cells in this phase = 36 36 Row C Time cell spends in this phase expressed as a decimal Row D Number of degrees in a circle graph for this phase 36 36 36 36 = 36/36 = 1.0 = 360o Title of Graph _______________________________ Key: Interphase Prophase Metaphase Anaphase Telophase Discussion: 1. In which phase of the cell cycle does a cell spend most of its time? _______________________ In which phase of the cell cycle does a cell spend the least time? _______________________ 2. Compare your pie graph to the cell cycle graph from your notes. ________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________