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AP: CHAPTER 20: DNA TECHNOLOGY
1. Define biotechnology.
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What is meant by “recombinant DNA technology?”
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Why are bacteria ideal workhorses for biotechnology?
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What are other organisms used in biotechnology?
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How does gene cloning differ from human cloning?
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7. Why is DNA cloning considered an important technology?
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8. What are plasmids?
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9. What is the function of restriction enzymes in bacteria?
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10, How do bacteria protect their DNA from the effects of the restriction enzymes?
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11. How do biologists make use of restriction enzymes?
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12. What is a genomic library?
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13. How is cDNA different from typical eukaryote DNA?
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14. Describe the steps involved in cloning a gene.
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15. How can transformed bacteria that carry genes of interest be identified and isolated from the
majority of non-transformed bacteria?
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16. What can be accomplished with Nucleic Acid Hybridization?
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17. What is the purpose of the Polymerase Chain Reaction?
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18. List some advantages & uses of the PCR technique.
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19. How are DNA fragments of different sizes separated?
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21. What does the technique of Southern Blotting accomplish?
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23. What was the goal of the Human Genome Project?
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27. In the futur~,DNA ~±ips may be used for regular diagnostics. What do the florescent spots
indicate when the chip is read?
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28. How can DNA technology be used to diagnose a carrier of a genetic disorder?
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29. What is the goal of gene therapy?
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31. What is currently used by the FBI to do a DNA fingerprint in a criminal investigation?
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32. What technique has been used to modify agricultural p nts?
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33. List a few of the traits that have been engineered into agricultural plants? Could any of these
pose an environmental threat?
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