Student Name…………………………… VCE Biology 2007 Unit 4 Topic Test 1−Heredity Student Answer Sheet Answer each Multiple Choice question by circling the appropriate letter. Use a pencil. If you make a mistake erase and enter the correct answer. Marks will not be deducted for incorrect answers. Write your answers to Short Answer Section in the space provided directly below the question. Multiple Choice Question 1 A B C D Question 2 A B C D Question 3 A B C D Question 4 A B C D Question 5 A B C D Question 6 A B C D Question 7 A B C D Question 8 A B C D Question 9 A B C D Question 10 A B C D Question 11 A B C D Question 12 A B C D Question 13 A B C D Question 14 A B C D Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity VCE Biology 2007 Unit 4 Topic Test 1−Heredity Multiple Choice Section Use the following information to answer questions 1 and 2. The semi-aquatic plant Triglochin procera is found in Northern Victoria and has a diploid number of 16. However, scientists have found that some forms of this plant can have multiple sets of chromosomes such as 32 or 64 chromosomes. As well, the plants with multiple sets of chromosomes can produce fertile offspring. Question 1. The forms of plant that have multiple sets of chromosomes are an example of: A. B. C. D. polygenics. polymorphism. polyploidy. polyteny. Question 2. Which statement best describes how the plants with multiples sets of chromosomes can produce fertile offspring. The plants with multiple set of chromosomes: A. B. C. D. are more fertile than plants that are diploid. undergo non-disjunction during meiosis. undergo disjunction during meiosis while plants that are diploid do not. are able to form homologous pairs during meiosis, which leads to the production of gametes that can unite during fertilization. Question 3. Which one of the following pedigrees most likely represents a sex-linked dominant pattern of inheritance? Shaded individuals have the trait. Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity 1 Question 4. The table in Figure 1 below shows the results of many crosses between homozygous red flowered snapdragon plants and homozygous white flowered snapdragon plants. Flower colour No. of offspring Red flower Pink flower 26 50 White flower 24 Figure 1 From the above information in Figure 1, one could conclude that pink flower colour in snapdragon plants is the result of: A. complete dominance. B. co-dominance. C. incomplete dominance. D. differentiation. Question 5. The process by which the cytoplasm of a cell divides is referred to as: A. B. C. D. cytogenetics. cytoplasmic inheritance. cytokinesis. cytolysis. Use the following information to answer questions 6, 7 and 8. In humans, there is a sex-linked recessive disorder in which the kidneys fail to respond to the hormone vasopressin. As a result, the kidney does not reabsorb water efficiently and large amounts of dilute urine are produced. Figure 2 below shows a pedigree of a family displaying the disorder and shaded individuals have the genetic disorder. Figure 2 2 Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity Question 6. What feature of the pedigree in Figure 2 provides evidence that this genetic disorder is a sexlinked recessive one? A. B. C. D. it is not possible for females to show a sex-linked recessive disorder. the high incidence of males that are affected by this genetic disorder. not all sons of a female affected by the genetic disorder will have the genetic disorder. not all of the children of a male and female with the genetic disorder will have the genetic disorder. Question 7. With regard to this genetic disorder, individual II-4 in the above pedigree must be: A. B. C. D. heterozygous. homozygous recessive. homozygous dominant. cannot be determined from the information given. Question 8. If individual III-1, who has no family history of this genetic disorder, has children with individual III-2, what is the chance that their children will have this genetic disorder? A. B. C. D. 0. ¼ ½ 1. Question 9. Following birth, each baby has a sample of blood that is taken to test for the genetic condition known as phenylketonuria. This condition affects both genders equally and can be found in babies of parents who do not show the condition. Which of the following best describes the mechanism of inheritance for phenylketonuria? A. B. C. D. sex-linked recessive. sex-linked dominant. autosomal dominant. autosomal recessive. Question 10. People suffering from Patau syndrome have three copies of chromosome 13, rather than two. The extra chromosome present is most probably the result of: A. B. C. D. crossing over during meiosis. non-disjunction during meiosis. independent assortment during meiosis. continuous variation among individuals. Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity 3 Question 11. An individual nucleotide found in DNA is made up of: A. B. C. D. a nitrogen containing base, ribose sugar and a phosphate group. uracil, deoxyribose sugar and a phosphate group. a nitrogen containing base, deoxyribose sugar and a phosphate group. nucleoside, deoxyribose sugar and a phosphate group. Question 12. Figure 3 below is the results of DNA fingerprinting in a paternity case. Individuals Male A Male B Male C Male D Mother Child Figure 3 From the DNA fingerprinting results in Figure 3 above, which male could be the biological father of the child? Male: A. B. C. D. D. C. B. A. Question 13. The transfer of plasmids, which contain foreign DNA, into a bacterial cell is referred to as: A. B. C. D. 4 transformation. transgenic. transduction. transamination. Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity Question 14. In honey bees, Apis mellifica, female bees have 32 chromosomes while male bees have 16 chromosomes. Both sexes are necessary for sexual reproduction to take place. From your own knowledge and the information provided, one could conclude that: A. B. C. D. in males gametes would be formed during meiosis. in females gametes would be formed during meiosis. gametes would not be formed by either males or females. in females gametes would not be formed during meiosis, since female bees develop from unfertilized eggs. Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity 5 Short Answer Section Question 1. DNA replication needs to occur so that cells can reproduce and be replaced after they die. Cells also have a mechanism to correct mistakes that can occur during DNA replication. If however a mistake in DNA replication is not corrected this may result in a spontaneous mutation. a. b. Which organs of the human body are at risk of a germline mutation? _____________________________________________________________________ (1 mark) Name the enzyme that separates the two strands of a DNA molecule prior to its replication. _____________________________________________________________________ (1 mark) Figure 4 below is a diagrammatic representation of how scientists believe the processes involved in turning a particular gene on or off can occur in bacteria. When substance X is absent a particular protein binds to the operator gene and stops it from functioning. This results in an enzyme that breaks down substance X not being made. DNA molecule control region with operator gene start of transcription protein code area stop transcription Figure 4 c. What name is given to the protein that binds to the operator gene? _____________________________________________________________________ (1 mark) d. Briefly explain what happens if substance X is present in large enough concentrations. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks) 6 Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity e. Explain why pre m-RNA is longer than the m-RNA that is released from the nucleus. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks) Total = 7 marks. Question 2. Biotechnology and modern genetic engineering involves the use of many different types of techniques; to not only manipulate the DNA of organisms but also to transfer DNA from one species to a different species. a. Name the part within a DNA molecule that forms a specific site for restriction enzymes to cut. _____________________________________________________________________ (1 mark) b. After the restriction enzymes have cut a single piece of DNA into a number of different sized pieces, explain how these different lengths of DNA can be separated. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) c. Briefly explain the two different ways that restriction enzymes may cut a section of DNA molecule. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks) d. Name the vector found in bacterial cells that can carry DNA fragments across cell membranes. _____________________________________________________________________ (1 mark) Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity 7 e. Explain how gene cloning differs from polymerase chain reaction. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks) Total = 7 marks Question 3. Pure breeding tall tomato plants(T) with hairy stems(H) were crossed with pure breeding short tomato plants(t) with hairless stems(h). All the F1 offspring were tall and hairy. The two genes for these two traits are on separate chromosomes. a. Write down all the phenotypes of the F2 generation if the F1 generation are intercrossed. _____________________________________________________________________ _____________________________________________________________________ (1 mark) b. How could a student determine if any tall and hairy tomato plants that she may come across are pure breeding or not pure breeding for both of these traits? _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (2 marks) c. Explain the meaning of the term crossing-over. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) d. What is the name given to the chromosomes that result from crossing-over? _____________________________________________________________________ (1 mark) 8 Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity e. A student crossed tomato plants with the genotypes ttHH and TThh. Is it possible to obtain offspring that are homozygous recessive from this cross? Explain. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) Total = 6 marks Question 4. Figure 5 below is a pedigree of a family that shows the inheritance of a particular genetic condition over a number of generations. Shaded individuals in the pedigree have the genetic condition. Figure 5 a. What is the pattern of inheritance of this genetic condition in the pedigree above? b. _____________________________________________________________________ (1 mark) Briefly explain your answer to question 4a. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity 9 c. Using the letters M and/or m write down the genotypes of individuals II-2, II-3 and II4. _____________________________________________________________________ (1 mark) d. If individual III-9 has a male partner without the genetic condition, but whose father did have the same genetic condition as shown in the pedigree. What is the chance that their children will also have the genetic condition? Explain. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) e. f. Once again using the letters M and/or m, explain what the genotype/s of individuals III-6 and III-7 are likely to be. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) Briefly explain the difference between Y-linked inheritance and the locus of a gene. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ (1 mark) Total = 6 marks End of Task 10 Lisachem Materials VCE Biology 2007 Unit 4 Topic Test 1–Heredity Suggested Answers VCE Biology 2007 Unit 4 Topic Test 1 Heredity Multiple Choice Section. 1C 2D 3D 4C 5C 6B 7A 8A 9D 10 B 11 C 12 A 13 A 14 B Short Answer Section. Question 1. a. Ovaries and testes. (1) b. Helicase. (1) c. Repressor protein. (1) d. When substance X is present in large enough concentrations it will bind to the repressor protein, releasing the repressor protein from the operator gene. (1) This then results in m-RNA being transcribed so that the enzyme which is involved in the breakdown of substance X can be produced. (1) e. The pre m-RNA molecule is a single stranded molecule that corresponds to both the introns and exons of the DNA molecule. (1) However, before the RNA molecule is released from the nucleus the introns are removed to form the m-RNA which therefore is not as long as the pre m-RNA molecule. (1) Question 2. a. Recognition sequence or cutting site. (1) b. Different size lengths of DNA are separated by a technique called electrophoresis which enables pieces of DNA to move in an electric field from negative to positive through a gel. The separation of different size DNA lengths occurs because shorter lengths travel faster and move further than longer lengths in a given time. (1) c. Some restriction enzymes cut the two strands of a DNA molecule at points directly opposite each other leaving no exposed or overhanging nucleotide bases and form cut ends of DNA that are blunt. (1) However, other restriction enzymes cut the DNA at one point while the cut on the second strand is at a different point and not directly opposite. This results in exposed nucleotide bases on both the ends of the DNA that are called sticky ends. (1) d. Plasmids. (1). e. In gene cloning, the gene or piece of DNA to be cloned is inserted into a living organism such as bacteria using a vector and many identical copies are made of the gene. (1) With polymerase chain reaction no organism or vector is used but rather a piece of DNA to be replicated is heated to separate the DNA strands. Then each strand is used as a template to make a complementary strand. This process is repeated many times to make many copies of the original piece of DNA.(1) Question 3. a. Tall and hairy, tall and hairless, short and hairy, short and hairless. (1) b. The student would perform a test cross by using short and hairless tomato plants. (1) When crossed with tall and hairy tomato plants, all the offspring would be tall and hairy if the tomato plants were pure breeding for both traits. If some of the offspring were not tall and hairy then the tomato plants would not be pure breeding for both traits. (1) c. Crossing-over is the exchange of the same segment or part of a chromatid between homologous chromosomes during prophase I of meiosis. (1) Suggested Answers VCE Biology 2007 Unit 4 Topic Test 1–Heredity 1 d. e. Recombinant chromosome. (1) No, it is not possible. Tomato plants with the genotype ttHH will produce gametes that are all tH while plants with the genotype TThh will produce gametes that are all Th. Therefore as a result all the offspring will be heterozygous with the genotype TtHh. (1) Question 4. a. Autosomal recessive. (1) b. The genetic condition is recessive because individuals III-1, III-2, III-3 and III-4 show the genetic condition, but neither do either of their parents. The condition is autosomal because individual II-4 does not show the condition but his mother does. (1) c. II-2 : Mm, II-3 : mm, II-4 : Mm. (1) d. If individual III-9 is recessive they will have the genotype mm. Her partner’s father had the condition therefore her partner’s genotype will be Mm. Therefore the chance that their children will have the genetic condition is ½. (1) e. Since both parents of individuals III-6 and III-7 do not have the genetic condition, but some of their children do, both parents are heterozygous (Mm). Therefore since individuals III-6 and III-7 do not show the condition they could both be either MM or Mm. (1) f. Y-linked inheritance refers to traits that are the result of genes located on the Y chromosome found in human males. The locus of a gene refers to a gene’s specific position on a particular chromosome. (1) 2 Suggested Answers VCE Biology 2007 Unit 4 Topic Test 1–Heredity