OHS017
OHS Risk Assessment and
Control Form
Risk assessment completed by: Jose Perdomo
Staff/student number: s3167678
Faculty/Division:
Medicine
Document number
STGCL.RA.52.1
School/Unit:
St George Clinical School
Initial Issue date
18/03/2010
Current version
1
Current Version
Issue date 18/03/2010
Next review date
18/03/2012
For additional information refer to the OHS Risk Assessment and Control Procedure, the OHS Risk Rating Procedure and the Hierarchy of Risk
Controls.
Risk Assessment title:
Functional titration of adenovirus using the 293A plaque assay
Step 1: Identify the activity
Describe the activity:
This Risk Assessment describes the correct procedures to be followed when calculating the titre of a virus preparation used a plaque assay based upon 293A
transformed human embryonal kidney cells.
Describe the location: This activity can only take place within the Certified PC2, OGTR approved facility on level 3, Research and Education Centre, St George
Clinical School
Step 2: Identify who may be at risk by
the activity
Those working in the same Lab area.
__________________________________________________________________________________________________________________________________________________________________________
_________
Page 1 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
Steps 3 to 7: Identify the hazards, risks, and rate the
risks
1. An activity may be divided into tasks. For each task identify the hazards and associated risks.
2. List existing risk controls and determine a risk rating using the UNSW Risk Rating Procedure.
3. Additional risk controls may be required to achieve an acceptable level of risk. Re-rate the risk if additional risk controls used.
Tasks
Handling of virus
Hazards
Associated risks
(Step 3)
(Step 4)
Biological
exposure
Handling of virus
outside of a Class II
Biohazard Safety
Cabinet risks
generating aerosol
particles which may be
inhaled or ingested
Risk rating with
existing controls *
Additional risk
controls required
Risk Rating with
additional controls *
(Step 5)
(Step 6)
(Step 7)
Existing risk controls

External and internal
PC-2 training is
mandatory

An SWP has been
prepared

All handling of virus
must be carried out
in a Class II
Biohazard Safety
Cabinet

Correct PPE must
be worn

Correct waste
disposal procedures
must be followed
C
L
R
3
E
M
(Apply the hierarchy
of risk controls)
C
L
R
__________________________________________________________________________________________________________________________________________________________________________
_________
Page 2 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
Melting 2% agarose
in microwave
Water bath

Burn hazard

explosion
Fire


Melting agarose
constitutes a burn
hazard and
explosion
Insufficient water
in water bath can
cause fire
* C = consequence

An SWP has been
prepared

Wear thermal
gloves to avoid
skin burns.

Loosen the lid of
agarose
container while
melting it in
microwave so
that there is no
entrapment of
gas or vapors
which may lead
to an explosion.

Make sure to add
sufficient water
into water bath

Turn water bath
off when it is not
in use.
2
3
D
E
L
M
L = likelihood R = risk rating from the UNSW Risk Rating Procedure
Step 8 Documentation and supervisor
approval
Completed by: Jose Perdomo (signature)
Authorised by: Prof Beng Chong (signature)
Date:18/03/2010
Reviewed by:
Authorised by:
Date:
Reviewed by:
Authorised by:
Date:
Reviewed by:
Authorised by:
Date:
__________________________________________________________________________________________________________________________________________________________________________
_________
Page 3 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
Step 9: Implement the additional risk
controls identified
Indicate briefly what additional risk controls from Step 6 above were implemented, when and by whom.
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Step 10: Monitor and review the risk
controls
It is important to monitor risk controls and review risk assessments regularly. Review is required when there is a change in the process, relevant
legal changes, and where a cause for concern has arisen. Reviews could be scheduled on an annual basis. If the risk assessment has substantially
changed a new risk assessment is warranted.
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Documentation
It is a requirement that legal and advisory documentation that supports this risk assessment be listed. Such documentation includes Acts,
Regulations, Australian Standards and Codes of Practice, where applicable.

UNSW OHS330 “Gene Technology Procedure”

AS/NZS 2243.3 “Safety in laboratories. Part 3: Microbiological aspects and containment facilities”

St George Clinical School “Laboratory hazardous waste disposal procedure”
__________________________________________________________________________________________________________________________________________________________________________
_________
Page 4 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6, 16/07/2008
UNSW Concise OHS Risk Rating Table
OHS697
What you need to do
1. Consider what can go wrong that can hurt someone
2. Determine what the most likely outcome would be - Consequences
3. Determine how likely those consequences are - Likelihood
4. Calculate the risk rating
5. Required action
CONSEQUENCES:
Severe
Major
Moderate
Minor
Insignificant
How severely could someone be hurt
death or permanent disability to one or more persons
hospital admission required
medical treatment required
first aid required
injuries not requiring first aid
LIKELIHOOD:
Almost certain
Likely
Possible
Unlikely
Rare
How likely are those consequences?
expected to occur in most circumstances
will probably occur in most circumstances
could occur at some time
is not likely to occur in normal circumstances
may occur only in exceptional circumstances
CONSEQUENCES
Insignificant
1
Minor
2
Moderate
3
Major
4
Severe
5
M
H
H
VH
VH
M
M
H
H
VH
Possible
C
L
M
H
H
VH
Unlikely
D
L
L
M
M
H
Rare
E
L
L
M
M
M
LIKELIHOOD
Almost
certain
A
Likely
B
Risk level
Very high
High
Medium
Low
Required action
Act immediately:
The proposed task or process activity must not proceed. Steps must be taken to lower the risk level to as
low as reasonably practicable using the hierarchy of risk controls.
Act today:
The proposed activity can only proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk controls must include those identified in legislation, Australian Standards, Codes of
Practice etc.
(iii) the risk assessment has been reviewed and approved by the Supervisor and
(iv) a Safe Working Procedure or Safe Work Method has been prepared.
(v) The supervisor must review and document the effectiveness of the implemented risk
controls.
Act this week:
The proposed task or process can proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk assessment has been reviewed and approved by the Supervisor and
(iii) a Safe Working Procedure or Safe Work Method has been prepared.
Act this month:
Managed by local documented routine procedures which must include application of the hierarchy of
controls.
_______________________________________________________________________________________________________
________
Page 5 of 5
UNSW Concise OHS Risk Rating Table
Effective date: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6,16/07/2008