Name: ____________________________ Homework/class-work Unit#7 photosynthesis and cellular respiration(25 points) Think and try every question. There is no reason for a blank response or an I don’t know. Any blanks will receive a zero. Every assignment must be done on a separate piece of paper. Each assignment must be complete, neat, in complete sentences and done on time for full credit. Any assignment may be used as a take home or pop quiz at any time. One missing or late assignment will lose 5 points, 2 will lose 15 points, 3 will be considered incomplete and given a zero. 1. Photosynthesis and cellular respiration reading: Date:______________________ Photosynthesis A great variety of living things on Earth, including all green plants, synthesize their food from simple molecules, such as carbon dioxide and water. For this process, the organisms require energy, and that energy is derived from sunlight. The figure shows the energy relationships in living cells. Light energy is captured in the chloroplasts of plant cells and used to synthesize glucose molecules. In the process, oxygen (O2) is released as a waste product. The glucose and oxygen are then used in the mitochondria of the plant and animal cell, and the energy is released and used to fuel the synthesis of ATP from ADP and P. In the reaction, CO2 and water are released in the mitochondria to be reused in photosynthesis in the chloroplasts. The process of utilizing energy to synthesize carbohydrate molecules is referred to as photosynthesis. Photosynthesis is actually two separate processes. In the first process, energy-rich electrons flow through a series of coenzymes and other molecules. This electron energy is trapped. During the trapping process, adenosine triphosphate (ATP) molecules and molecules of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) are formed. Both ATP and NADPH are rich in energy. These molecules are used in the second half of the process, where carbon dioxide molecules are bound into carbohydrates to form organic substances such as glucose. Chloroplasts The organelle in which photosynthesis occurs (in the leaves and stems of plants) is called the chloroplast. Chloroplasts are relatively large organelles, containing a watery, protein-rich fluid called stroma. The stroma contains many small structures composed of membranes that resemble stacks of coins. Each stack is a granum (the plural form is grana). Each membrane in the stack is a thylakoid. Within the thylakoid membranes of the granum, many of the reactions of photosynthesis take place. Chlorophyll Pigment molecules capture sunlight in the chloroplasts. In the reaction center there are about 200 molecules of a green pigment called chlorophyll and about 50 molecules of another family of pigments called carotenoids. In the reaction center, the energy of sunlight is converted to chemical energy. The center is sometimes called a light-harvesting antenna. The energy captured in the reaction centers drive the production of ATP in the chloroplasts. The process of photosynthesis The process of photosynthesis is conveniently divided into two parts: the energy-fixing reaction (also called the light reactions) and the carbon-fixing reaction (also called the light-independent reaction or the Calvin cycle) Light reactions (energy-fixing reactions) The light reactions of photosynthesis begin when light is absorbed in the thylakoid membranes. The energy of the sunlight captured in the reaction center, activates electrons to jump out of the chlorophyll molecules. These electrons pass through a series of cytochromes in the nearby electron-transport system. After passing through the electron transport system, the energy-rich electrons eventually reduce NADP. This reduction occurs as two electrons join NADP and energize the molecule. Because NADP acquires two negatively charged electrons, it attracts two positively charged protons to balance the charges. Consequently, the NADP molecule is reduced to NADPH, a molecule that contains much energy. Electrons that are lost during the reduction of NADP into NADPH are replaced by taking them from the water molecules. Each split water molecule releases two electrons that enter the chlorophyll molecules to replace those lost. Another product of the split water molecules is oxygen. Two oxygen atoms combine with one another to form molecular oxygen, which is given off as the byproduct of photosynthesis. Oxygen fills the atmosphere and is used by all oxygen breathing organisms, including plant and animal cells. The ATP production in the energy-fixing reactions of photosynthesis occurs by the process of chemiosmosis. Essentially, this process consists of a rush of protons across a membrane (the thylakoid membrane, in this case), accompanied by the synthesis of ATP molecules. Biochemists have calculated that the proton concentration on one side of the thylakoid is 10,000 time that on the opposite side of the membrane. ATP is formed in the energy-fixing reactions along with the NADPH formed in the main reactions. Both ATP and NADPH provide the energy necessary for the synthesis of carbohydrates that occurs in the second major set of events in photosynthesis. Light independent reactions (carbon-fixing reactions) Glucose and other carbohydrates are synthesized in the carbon-fixing reaction of photosynthesis, often called the Calvin cycle for Melvin Calvin, who performed much of the biochemical research. This phase of photosynthesis occurs in the stroma of the plant cell. In the carbon-fixing reaction, an essential material is carbon dioxide, which is obtained from the atmosphere. The carbon dioxide is attached to a five-carbon compound called ribulose diphosphate. A series of reaction convert the newly formed six-carbon compound into glucose by using the energy in ATP and NADPH made during the light dependent reactions. Glucose can be stored in plants in many ways. In some plants, the glucose molecules are joined to one another to form starch molecules. Potato plants, for example, store starch in tubers (underground stems). In some plants, glucose converts to fructose (fruit sugar), and the energy is stored in this form. In still other plants, fructose combines with glucose to form sucrose, commonly known as table sugar. Plant cells obtain energy for their activities from these molecules. Animals use the same forms of glucose by consuming plants and delivering the molecules to their cells. All living things on Earth depend in some way on photosynthesis. It is the main mechanism for bringing the energy of sunlight into living systems and making that energy available for the chemical reactions taking place in cells. Cellular respiration Organisms, such as plants, can trap the energy in sunlight through photosynthesis and store it in the chemical bonds or carbohydrates molecules. The principle carbohydrate formed through photosynthesis is glucose. Other organisms, such as animals, fungi, protozoa, and a large portion of bacteria, are unable to perform this process. Therefore, these organisms must rely on the carbohydrates formed in plants to obtain the energy necessary for their metabolic processes. Animals and other organisms obtain the energy available in carbohydrates through the process of cellular respiration. Cells take the carbohydrates into their cytoplasm, and though a complex series of metabolic processes, they break down the carbohydrates and release the energy. The energy is generally not needed immediately; rather it is used to combine adenosine diphosphate (ADP) with phosphate ions to form adenosine triphosphate (ATP) molecules. The ATP can then be used for processes in the cells that require energy, much as a battery powers a mechanical device. During the process of cellular respiration, carbon dioxide is given off. This carbon dioxide can be used by plant cells during photosynthesis to form new carbohydrates. Also in the process of cellular respiration, oxygen gas is required to serve as an acceptor of electrons. This oxygen is identical to oxygen gas given off during photosynthesis. Thus, there is an interrelationship between the process of photosynthesis and cellular respiration, namely the entrapment of energy available in sunlight and the provision of the energy for cellular processes in the form of ATP. Reading Questions: 1. Do plants and animals both go through photosynthesis? Why or why not? Do both plants and animals go through cellular respiration? Why or why not? 6. Briefly describe what occurs during the light dependent reaction? 8. What is NADPH? What is its role in the process of photosynthesis? 9. Where does photosynthesis take place? What molecules absorb sunlight? 10. Describe the physical structure of the chloroplast. 11. Briefly describe what happens in the light independent reactions? 14. Do plants produce oxygen? Explain. 4. Photosynthesis and advanced questions: Date: ____________________ 1. How do plants get energy from their environment? 2. When animals shiver in the cold, muscles move almost uncontrollably. Suggest how shivering helps as animal survive in cold. 3. Elodea springs were under white light, and the rate of photosynthesis was measured by counting the number of oxygen bubbles per minute for ten minutes. Predict the rate of photosynthesis if a piece of red cellophane were placed over the white light. 4. A window plant native to the desert of South Africa has leaves that grow almost entirely under ground with only the transparent tip of the leaf protruding above the soil surface. Suggest how this adaptation aids the survival of this plant. 5. Why would you not expect to find mitochondria in anaerobic organisms? 6. Cells of an onion are clear (white) why? The cells of an onion do not go through photosynthesis. Why? Only some cells in a plant have chloroplast but all cells in a plant have mitochondria. Why? 7. Write the chemical equation for photosynthesis: A. Tell where each molecule is used or made. B. Briefly explain how each is used or made. 8. Which process do you think plants carry out faster, photosynthesis or respiration? Why? 5. Review: Date: _______________ 1. An organism that makes it’s own food. 2. What does ATP stand for? 3. Does ATP or ADP have more energy? 4. What does ROY G. BIV stand for? 5. Plants have light-absorbing substances called. 16. Where does the Calvin cycle take place? 17. What are the two starting materials for the Calvin cycle? 18. What two compounds made in the light reactions are used during the Calvin cycle?