PROF.DR. SHAGUFTA NAZ Chair in Biotechnology and Microbiology Department. ADDRESS: Department of Biotechnology and Microbiology, Lahore College For Women University,Lahore, Pakistan. EDUCATION: 1996 1987 1984 PhD, Botany (Biotechnology), University of Punjab, Lahore. MSc, Botany, University of the Punjab, Lahore BSc, Botany,Zoology,Geography,Bahhudin Zikryu University, Multan. PROFESSIONAL EXPERIENCE: 2010 - Present 2009 - 2010 2005 - 2009 2002- 2005 1996- 2002 Pakistan. Professor and chair in the Department of Biotechnology& Microbiology, Lahore College for women University, Lahore,Pakistan. Associate Professor, Botany Department, Lahore College for Women University, Lahore, Pakistan. Assistant Professor, Botany Department, Lahore College for Women University, Lahore, Pakistan. Senior Biotechnologist, Kissan Services and Supplies, Pvt.Ltd, Lahore, Pakistan. Director Research & Development, Agriculture Biotechnologies Pvt. Ltd Lahore, UNIVERSITY SERVICE: Professor and chair in Biotechnology &Microbiology Department to present. LCWU, Lahore Coordinator of MS and Ph.D Program, Lahore College for Women University, Lahore. Convener of Garden Committee, LCWU, Lahore Member of Doctoral committee, LCWU,Lahore Member of Equillance committee, LCWU,Lahore. Member Campus Committee,LCWU,Lahore Member, Senior Faculty forum, LCWU,Lahore. Member Purchase Committee, LCWU,Lahore Member, Academic Council of LCWU, Lahore Convener, Selection Board grade 1-16, LCWU, Lahore From 2005 to 2009 worked as coordinator in examination of Botany Department, LCWU Lahore. From 2005 to 2009 worked as member Purchase Committee of Botany Department, LCWU, Lahore. From 2006 to 2007 worked as Member Disciplinary Committee, LCWU,Lahore. Worked as Focal Person in curriculum development of MS leading PhD program in Botany Department. Appointed as Management Representative in quality assurance program (ISO 9000) in Botany Department, LCWU, Lahore. 1 PROFESSIONAL ORGANIZATIONS/AFFILIATIONS: Pakistan Botanical Society. Bangladesh Botanical Society. Kitchen Garden Society. Member, Advisory Committee of Baghe-Jinnah,Lahore Member, Biotechnology Committee, Lahore Chamber of Commerce, Lahore Member, Horticulture Society, Lahore INTERNATIONAL TRAINING ATTENDED: Attended one month extensive training under HEC Program “Pakistan Program for Collaborative Research (PPCR) Foreign Visit of Local Faculty Member” in Department of Viticulture & Enology at the University of California, Davis, USA in year 2010 with Dr.Andrew Walker.In this period experience was gained experience in DNA marker technology with simple sequence repeat markers, from extraction, to PCR amplification and allele size analysis. Training was also achieved in the grape transformation techniques in the lab. After this training I started work in my Department and tried to establish this system there. Attended another trainin in Oct.2012 in the laboratory of Dr.Andrew Walker at the University of California,Davis, and gained experience on new applied bio-system 3500 genetic analyzer .I got proper training on the effective operation of the Applied Biosystems 3500 Genetic Analyzers fragment analysis applications, to maximize throughput and obtain high quality data. After this training I am able to demonstrate knowledge of fragment analysis technology and instrumentation, and choose the most appropriate chemistry for a given application. and to identify methods of fluorescent labeling of fragments, prepare and load samples, set up and run the instrument, use collection and analysis software “Gene Mapper” to analyze and genotype the data Publications 1. K. Shahzadi, S. Naz, A. Tariq, S. Ilyas, M. A Qadeer* and I. U. Haq (2012) Production of calcium gluconate by aspergillus niger gcu-1 Pakistan Journal of Science Vol. 64 No. 3 :214-218 2. Shagufta Naz1, Fozia Naz*, Amina Tariq1, Farah Aslam1, Aamir Ali2, and Mohammad Athar3 (2012) Effect of different explants on in vitro propagation of gerbera (Gerbera jamesonii) African Journal of Biotechnology Vol 11(37):.9048-9053. 3. Shagufta Naz, Farah Aslam, Saiqa Ilyas, Kiran Shahzadi and Amina Tariq(2012) In vitro propagation of tuberose (Polianthes tuberosa) Journal of Medicinal Plants Research Vol. 6(24), pp. 4107-4112, 28 June, 2012. 2 4. Sana Rashid, Saiqa. Ilyas, Shagufta. Naz, Farah. Aslam And Aamir Ali (2012) In vitro propagation of gypsophila paniculata through plant tissue culture techniques. Pakistan Journal of Science:Vol. 64 No. 1,6-10. 5. Farah. Aslam, Sana. Habib and Shagufta. Naz (2012) Effect of different phytohormones on plant regeneration of amaryllis hippeastrum. Pakistan Journal of Science: Vol. 64, No. 1:54-60. . 6. Saiqa Ilyas, Shagufta Naz, Sumeera Javad, Kiran Shehzadi, Amna Tariq, Neelma Munir (2012) Influence of cytokinins, sucrose and pH on adventitious shoot Regeneration of Polyscias balfouriana (Balfour aralia) Journal of Medicinal Plants Research: Vol. 5(xx), pp. xxx-xxx 18 March, 2011 Available online at http://www.academicjournals.org/JMPR DOI:10.5897/JMPR11.1652 ISSN 1996-0875 ©2011 Academic Journals 7. Sumera Javad ,Shagufta Naz, Saiqa Ilyas, Aamir Ali, Farah Aslam and Neelma Munir(2012) A study of the effect of Physical state of medium and different concentrations of Sucrose, FeEDTA and CuSO4 to enhance micropropagation system of Stevia rebaudiana. Journal of Medicinal Plants Research, Vol. 6(9), pp. 1800-1805. 8. Aamir Ali1, Irum Gull 2, Abdul Majid, Asif Saleem1, Namia Huma Naveed1and Shagufta Naz3 (2012) In vitro conservation and production of vigorous and desiccate tolerant synthetic seeds in Stevia rebaudiana Journal of Medicinal Plants Research Vol. Vol. 6(7), pp.1327-1333. 9. Farkhanda Manzoor, Neelma Munir, Asma Ambreen and Shagufta Naz (2012) Efficacy of some essential oils against American cockroach Periplaneta americana (L) Journal of Medicinal Plants Research Vol. 6(6), pp. 1065-1069 . 10. Shagufta Naz, Erum Akbar, Safia Jabeen, Saiqa Ilyas (2012) Antibacterial activity of different varieties of Curcuma longa rhizome extracts against pathogenic bacteria. Asian Journal of Chemistry. Volume 24, No. 9 ; 2875-2877 11. Aamir Ali, Anum Sajid, Naima Huma Naveed, Abdul Majid, Asif Saleem, Umair A. Khan,Fisal Iqbal Jafery and Shagufta Naz (2011) Initiation, proliferation and development of micropropagation system for mass scale production of banana through meristem culture African Journal of Biotechnology: 10(70): 15731-15738, 9 12. Shagufta Naz, Fozia Tabassum, Sumera Javad, Saiqa Il yas, Farah Aslam, Neelma Munir1 and Aamir Ali(2011)Micropropagation and callogenesis of a recalcitrant species Ricinus communis. Pakistan Journal of Botany. 43(5) 2419-2422. 13. Shagufta Naz, Saiqa Ilyas, Safia Jabeen, and Zahida Parveen (2011) Composition and anti-bacterial activity of the essential oil from the rhizome of turmeric (Curcuma longa L. Asian Journal of Chemistry :Volume 23, No. 4 :1639-1642. 3 14. Farkhanda Manzoor , W.Beena,Saadiya Malik , Narjis Naz, , Shugufta Naz and W.H.Syed(2011) Preliminary evaluation of Ocimum sanctum as toxicant and repellent against termite,Heterotermes indicola (Wasmann)(Isoptera:Rhinotermttidae) . Pakistan Journal of Science: Volume 63, No1: 59-62. 15. Farkhanda Manzoor , Saadiya Asma Malik , Narjis Naz, K.J.Cheema, Shugufta Naz (2011) Potential of Antitermitic Activities of Eucalyptus Oil . Pakistan Journal of Zoology: 2011. Issue. 16. AAMIR ALI, IRUM GULL, SHAGUFTA NAZ (2010) Biochemical investigation of protein and peroxidase activity during different stages of In vitro propagation of Stevia rebaudiana. Pakistan Journal of Botany. 42(4): 2827-2837. 17. Shagufta Naz, Safia Jabeen, Saiqa Ilyas, Farkhanda Manzoor, Farah Aslam and Aamir Ali(2010) Antibacterial activity of curcuma longa varieties against different strains of bacteria. Pakistan Journal of Botany. 42(1): 455-462. 18. Shagufta Naz , Saiqa Ilyas, Zahida Parveen and Sumera Javed.(2010) Chemical analysis of essential oils from turmeric (Curcuma longa) rhizome through GC-MS Asian Journal of Chemistry. Volume 22. (4): 3153-3158. 19. Shagufta Naz, Saiqa Illyas, Sumera Javed, and Aamir Ali (2009) In vitro clonal multiplication and acclamitization of different varieties of turmeric (curcuma longa L.) Pakistan Journal of Botany .Volume No. 41(6) 2807-2816. 20. . Shagufta Naz, Sumera Javed, Saiqa Illyas and Aamir Ali (2009) An efficient protocol for rapid multiplication Bryophyllum Pinnatum and Bryophyllum daigrimontianum.Pakistan Journal of Botany:41(5)2347-2355. 21. Shagufta Naz, Fayyaz Ahmed Siddique, Aamir Ali and Javed Iqbal (2009) Virus indexation of in vitro regenerated sugarcane plants. Pakistan Journal of Botany. 41(4) 1931-1939. 22. Shagufta Naz, Aneela Hashmi and Aamir Ali (2009) In Vitro Callogenesis and Organogenesis in different explants of Stevia ( Stevia Rebaudiana) Pakistan Sugar Journal.XXIV(2)20-26. 23. Shagufta Naz, Aamir Ali, and Javed Iqbal (2008) Phenolics in in vitro cultures of chick pea during callogenesis and organogenesis. Pakistan Journal of Botany 40(6) 2525-2539 24. Shagufta Naz, Aamir Ali, Fayyaz Siddique and Javed Iqbal (2008) Somatic embryogenesis from immature cotyledons and leaf calli of chick pea (Cicer arietinum L.) Pakistan Journal of Botany .40(2) 523-531. 4 25. Shagufta Naz, Aamir Ali and Aqsa Siddique (2008) Somatic Embryogenesis and plantlet formation in different varieties of sugarcane (Saccharam officinarum L.) Sarhad Journal of Agriculture.24 (4)593-598. 26. Aamir Ali, Shagufta Naz, Fayyaz Siddique and Javed Iqbal (2008) An efficient protocol for large scale production of sugarcane through micropropagation. ) Pakistan Journal of Botany .40(1)139-149. 27. Aamir Ali, Shagufta Naz, Fayyaz Siddique and Javed Iqbal (2008) Rapid clonal multiplication of sugarcane ( Saccharam officinarum L.) through callogenesis and organogenesis) Pakistan .J of Botany. 40(1):123-138. 28. AamirAli, Humera Afrasiab, Shagufta Naz, Mamoona Rauf and Javed Iqbal (2008) An efficient ptotocol for in vitro propagation of carnation (Dianthus Caryophyllus) ) Pakistan Journal of Botany 40( 1)111-121. 29. Shagufta Naz and G.Zahara Jahangir (2008) Effect of medium composition on callogenesis and somatic embryogenesis in different varieties of sugarcane (Saccharam officinarum L.) i.e S 2002US-302, HSF-240 and HSF-242. Pakistan Sugar Journal: Vol.XXIII, No.02:-11-20. 30. Shagufta Naz, Aamir Ali, Fayyaz Siddique and Javed Iqbal (2007 Multiple shoot formation from different explants of chick pea (Cicer arietinum L.) Pakistan Journal of Botany. 39(6)2067-2073. 31. Aamir Ali, Asifa Munawar, Shagufta Naz (2007) An In Vitro study on micropropagation of Caladium bicolour International J. of Agriculture &Biology15608530/09-5731-735 32. Aamir Ali, Shagufta Naz, and Javed Iqbal (2007) Effect of different explants and media compositions for efficient somatic embryogenesis in Sugarcane (Saccharam officinarum L.) Pakistan Journal of Botany 39(6):1961-1977. 33. Aamir Ali,Shagufta Naz ,Sarwar Alam and Javed Iqbal (2007) In Vitro induced mutation for screening of red rot(Colletotrichum falcatum) Resistance in Sugarcane (Saccharam officinarum L.) Pakistan Journal of Botany.39 (6)1979-1994. 34. Naz. S (2004) Micro Propagation of Promising varieties of Sugar cane and their acclimatization response. Pakistan Sugar Journal vol.XIX 02; 58-64. 35. Ali.A, Naz.S, Siddique.F.A and Iqbal.J (2001) Callogenesis, emmbrogenesis and Organogenesis in CHRISMIS CACTUS (SCHLUMBERGERA BRIDESH) Pakistan Journal of Botany. 33(Special issue) 569-575. 5 36 . Ali. A, Naz.S, Siddique.F.A and Iqbal.J (2001) IN VITRO Propagation of GLOXINIA (SINNIGIA SPECIOSA) Pakistan Journal of Botany.33 (Special issue) 575-579. 37. Naz, S. Siddique, F. A and Iqbal, J, (1993) In vitro flowering of Hyacinths. Advances in plants tissue culture 1:40-42 38. Naz, S. Siddique, F. A and Iqbal, J, (1993) In vitro propagation of carnation. Advances in plants tissue culture 1:43-47 39. Naz, S. Siddique, F. A and Iqbal, J, (1993) Micropropagation of roses. Advances in plants tissue culture 1:48-54 40. Iqbal, J, Naz, S., Nasir, S, Aftab, F and Ahmad, M.S.1991. Total phenolics, phenylalanine ammonia lyase and polyphenol oxidase in in vitro calli of chickpea. Pakistan Journal of Botany.23(2): 227-235 41. Iqbal, J and Naz, S.1989. Effects of Barium on the germination. Soluble protein and isozymic forms of peroxides in wheat. Pakistan Journal of Agri. Res., 10 (1)15-22 Published Books 1. Micropropagation of Bulbous Plants (2012) Farah Aslam, Shagufta Naz. 2. Micropropagation and antimicrobial activity of Turmeric (2012) Safia Jabeen, Shagufta Naz. 3. Callogenesis and Micropropagation of Grape (Vitis vinifera L.) and Biochemical investigation of Protein Content and Peroxidase activity during different Stages of Growth. (2012) Shagufta Naz and Khadija Gillani 4. Comparison of in vitro Response of Micropropagation, Callogenesis and Antimicrobial activity of Vinca rosea L. Rukhama Haq and Shagufta Naz 5.Influence of Growth Regulators and sucrose Concentration on Micropropagation, Callogenesis and Antimicrobial activity of Amaryllis belladonna L. Books in Press: 1. Effect of different explants and media compositions for efficient growth somatic embryogenesis, callogenesis and micropropagation of three varieties (NSG-59, Qsg-1741 and HSF 240) of Saccharum officinarum L. 2. Callogenesis, Organogenesis and Antimicrobial activity of Clitoria ternata L 6 Publications in Press: 1. Aamir Ali,Asif Saleem and Shagufta Naz (2012) Molecular characterization and the phylogenetic relationship among different sugarcane (Saccharum officinarum L.) varieties of Pakistan. submitted to Gen. Mol. Res., Plant Cell Report. Theo. Appl. Genet., 2. Shagufta Naz and Saiqa Ilyas (2012) Use of the polymerase chain reaction (PCR) and random amplification of polymorphic DNAs (RAPDS) for the determination of genetic diversity between 21 Chrysanthemum varieties of Pakistan. Submitted to. Theo. Appl. Genet. 3. Shagufta Naz, Ambreen Zafrullah and Saiqa Ilyas (2012) Use of RAPD marker for the assessment of genetic diversity in Tomato cultivars. Submitted to Gen. Mol. Res. 4. Shagufta Naz, Sana Rashid and Kiran Shahzadi (2012) Molecular characterization and phylogenetic relationship of different citrus varieties of Pakistan. Submitted to Gen. Mol. Res.and Pakistan J. of Botany. Dissertation title 1- Ph.D. “Phenolics in in-vitro cultures of chickpea during callogenesis and organogenesis” 2- M.Sc. Effects of Barium Chloride on the germination. Soluble protein and isozymic forms of peroxidases in wheat. Employment record: I. Professor (2010-To date) Presently working as a Professor and Head of department in Biotechnology & Microbiology Department, Lahore College for Women University, Lahore from April.2010 to date II. Associate Professor (2009-2010) Worked as an Associate Professor in Botany Department, Lahore College for Women University, Lahore from Jan.2009 to April 2010. III. Assistant Professor (2005-2008) Worked as an Assistant Professor in Botany Department from Oct .2005 to 31st Dec.2009. 7 In Associate and at the post of Assistant Professor in same university following services were rendered to the organization: Two complete Tissue Culture laboratories including designing, procurement and installation of equipment and initiation of different varieties of cultures were established in Lahore College for Women University and in Baghe-Jinnah Lahore. Optimized protocols and established various plants under in-vitro conditions, including cash crops like Turmeric, Bananas, Sugarcane, Potato, ornamentals i.e. Lilium Orchid, Carnation, and medicinal plants –Stevia, Bryophyllum, Vinca, Ricinus etc. Established and optimized the methods of isolation of oleoresins and active ingredients from different plant species both in vivo and in vitro by using different isolation techniques. These isolated compounds were analysed and characterized by using spectrophotometer and GCMS techniques. Determined antimicrobial activities of these isolated compounds against different strains of bacteria and fungus. III: Consultant(2003-2005) Worked as consultant for establishment of plant tissue culture lab. for private seed propagation company (Kissan Services and Supplies). A complete project from establishment of lab to field was established and operated successfully for production of disease free Potato, Sugarcane and vegetable seeds through micropropagation at commercial level IV:Director Production (July1996-Aug2002: Established first commercial Plant Tissue Culture lab in private sector in Pakistan famous as Agri Bio Tech and worked as Director Production of Agriculture Biotechnologies (pvt) Ltd Lahore from 1996-2002.In this period the following activities were performed Establishment of Joint Research Collaborations/MOU: 1. Established a broad formal relationship between Public university i.e. Lahore College for Women university, Lahore and a private university i.e. The University of Lahore, Lahore (documented by complete MOU) for facility for undertaking research in genetics and biotechnology to the faculty and students involved in Agricultural biotechnology teaching and research. 2. An agreement has been initiated with the Agriculture Department, Govt. of Punjab, Lahore and Lahore College for Women University, Lahore for collaborative research in the field of Biotechnology particularly in plant tissue culture. Promising and costly ornamental varieties of different plant families will be developed by different plant tissue culture techniques and 8 will be multiplied at lab, green house and field levels and supplied to the national and international market. Projects In Public Sector Awarded A project entitled “Development of turmeric production technology for enhancement of yield, curcumin and oleoresin content using tissue culture technology was submitted to HEC under the National Research Program for Universities on 6th Sep.2006. The project was awarded in July 2007. The total cost of project is 3.5 million. According to MOU signed with Agriculture Department, Govt. of Punjab, a complete Plant Tissue Culture lab. along with green houses has been established and run with the collaboration of both organizations. Now the lab has been handed over to LCWU and we have equipped the lab and now it is in full operational position. Another project entitled “In vitro conservation of some medicinally important endangered, threatened and near to extinct species of different ecological zones of Pakistan” was submitted to HEC and it was awarded in July, 2010. The total cost of project is 2.7 million. As a Co.PI two project s were awarded in 2011. The total cost of project is 1.0 million. Research Grant under HEC Program “Pakistan Program for Collaborative Research (PPCR) Foreign Visit of Local Faculty Member” was awarded. The amount of grant is 253,265 Rs. PC1 Submitted in Public Sector Submitted PC- 1 of Botany department under the program of Submitted PC-1 of Establishment of Plant Tissue Culture lab. in Bagh-e Jinnah as a part of “Rehabilitation/ Up-Gradation of Existing Auditorium and Provision of Labs and Library “STRENGTHENING OF BOTANY DEPARTMENT, LAHORE COLLEGE FOR WOMEN UNIVERSITY, LAHORE” Capital cost: Rs. 15 million Facilities at LCWU, Lahore (Prime Minister's Directive)” Capital Cost: Rs. 5.6 Million 9 Submitted PC- 1 of Biotechnology and Microbiology department under the program of “Establishment (Provision of Lab.Equipment) of newly built Biotechnology Department of Lahore College for Women University, Lahore” Capital Cost: Rs.58.007 Million Project Reports Submitted: One year Project report of HEC Awarded Project: Submitted the one year progress report of the project entitled “Development of turmeric production technology for enhancement of yield, curcumin and oleoresin content using tissue culture technology to the Higher Education Commission of Pakistan 2nd and Final year Project report of HEC Awarded Project: Submitted the 2nd and final year progress report of the project entitled “Development of turmeric production technology for enhancement of yield, curcumin and oleoresin content using tissue culture technology to the Higher Education Commission of Pakistan A Report of Research Grant under HEC Program” Pakistan Program For Collaborative Research (PPCR)” Foreign Visit of Local Faculty Member to USA was also submitted to HEC. Submitted the one year progress report of the project entitled “In vitro conservation of some medicinally important endangered, threatened and near to extinct species of different ecological zones of Pakistan” to the Higher Education Commission of Pakistan Submitted projects A project entitled “Development of a core facility to evaluate the genetic diversity of important fruit crops in Pakistan and development of DNA fingerprint databases to manage germplasm collections” was submitted to The Third World Network of Scientific Organizations (TWNSO).The requested amount of the project is 29,600 US Dollar. A project entitled “Development of a core facility to evaluate the genetic diversity of important fruit crops in Pakistan and development of DNA fingerprint databases to manage germplasm collections was also submitted to the National Science Foundation, Directorate for Biological Sciences (DCC-PGR), USA under the program of Developing Country 10 Collaborations in Plant Genome Research. The requested amount of the project is 98,000 US Dollar. A strengthening Project entitled “Establishment of Bio-informatics Laboratory in Department of Biotechnology &Microbiology” was also submitted to the HEC for funding. The budget of the project is 1.0 Million Rs. A project entitled ‘Conservation of some important,endangered,threatened and near to extinct species of medicinal plants using different in vitro techniques to the CRP-ICGEB. The requested amount of the project is 73,000 Euro. In this duration, teaching as well as research was conducted at different levels: Teaching Experience: Classes Year Courses Duration B.Sc Hons Environmental Sci. B.Sc Hons Education B.S Hons Botany Two semester 2006 Biodiversity 2006-2007 General science 2006-2007 Cell biology, Biochemistry, Genetics Same as above, Research Methodology One semester May-June 2006 Two semesters Oct.06-Aug07 Two semesters Aug.06-June 07 2007-2008 Seventh semester M.Sc I 2006-2007 Plant Tissue Culture 2007-2008 Same as above M.Sc II&MS 2007-2009 Research Methodology MS 2010 -2012 MS Leading PhD 2009-2011 PhD 2007-2011 2008-2011 2008-2012 Sep.07-June -08 Two semesters Oct.06-Aug07 Oct.07-Aug.08 One semester Sep.07 Feb.08 Plant Tissue Culture Two Semester Project Planning &Monitoring Nov.10 July 11 Research Reading Two semesters Biostat &Bioinformatics Plant Cell Culture Two semesters Plant Biotechnology Two semesters Application and Techniques of Molecular Biology 11 Research Reading Application of Plant Tissue Culture Research: Classes Year Research Duration Status Discipline Six students of MSc II Oct.05-Sep.06 Two students of M.Sc II Sep.07-Aug.08 Three students of Sep.08- Aug.09 Plant tissue culture One year Plant tissue culture Supervisor One year Supervisor Plant tissue culture & Biochemistry One year Supervisor Plant tissue culture One year Supervisor Plant tissue culture One year & Biochemistry Plant tissue culture One year Molecular Genetics Supervisor M.S and 3 of BSIV Two students of MS and 3 of BSIV Sep.09-Aug.2010 &Bioassay Techniques Five students of MS Sep.10-Aug.2011 Five students of MS Sep.11-Aug.2012 One PhD HEC Scholarship Awarded Student 2007-2011 Biochemistry Four year Supervisor Co-supervisor Three PhD Student 2 -HEC Scholarship Awarded Student 2009-2013 Biotechnology Four years Supervisor Two PhD Student HEC Scholarship Awarded Student 2010-2014 Biotechnology Four years Supervisor : 12 A:Development and optimization of commercial protocols for Lab, Green house and Field propagation of various commercial crops; 1: Commercial production of disease free seeds (1996-2005) Commercial production of disease free seeds of potato and sugarcane through tissue culture technology. 2: Micropropagation of plants(1997-2004) Standardized the protocols for Micro propagation of ornamental as well as flowering plants like carnation, begonias, Spathyphylum, Gladioli and Aloe Vera .etc. 3: Ornamental propagation in Green house (1997-2002) Various propagations were optimized in the controlled environment of Green House for off season as well as quality production of Ornamental like Petunias, carnations, gerbera, dahlia, gloxinia, bulbs and various other plants. 4: Orchid Propagation (1998-2000) Both in vivo and in vitro propagation of wide range of Orchids including local, exotic and hybrid varieties has been optimized. B:Development of commercial protocols for Green house ,off season vegetable seedlings and commercial crop production 5: Commercial seedling production (1998-2001) Optimized the techniques for commercial seedling production from hybrid seeds of tomato, hot and sweet chilies, cucumber and watermelon. 6: Off season vegetable production (1999-2001) Most actively participated in developing the hot houses in different ecological zones of Pakistan for commercial production of off season vegetables i.e. Tomato, hot and Sweet pepper, Cucumber and vegetables of Cucurbitace family. C: Post harvest preservation of vegetable and fruits through dehydration process 7: Food Dehydration ( 2001-2002) Post harvest technology and food dehydration including fruit and vegetable for local and export market for value addition and crop conservation. Advance food ingredients developed in collaboration with Best Food, Value Chicken and Nestlé Pakistan. A: Plant Tissue Culture (1987-1996) Plant cell and tissue culture projects were conducted in laboratories of Botany Department, University of the Punjab, Lahore and Nuclear institute of Agriculture and Biology (NIAB) Faisalabad as given below ; 13 I. Chick-pea: (1986—1988) Introduction of blight resistance in chick pea via somaclonal variation (sponsored by Pakistan Atomic Energy commission 19861989). II. Sugarcane;(1990-1993)Development of sugarcane cell suspension and protoplast culture.(sponsored by National Scientific Research and Development Board) III. Ph.D. Thesis(1991-1996): entitled “Phenolics in in-vitro cultures of chickpea during calogenesis and organogenesis” IV. Micro propagation(1988-1996):Various protocols were optimized for in vitro propagation of commercially important plants like roses, carnation,Hyacinth, chroton, Dahlia, Falcatus and Begonia.(sponsored by Punjab University, Lahore) V. Agriculture Biotechnologies (pvt) Ltd( 1996-2003)Experience gained by the direct participation in establishment of complete tissue culture laboratory , Glass houses, Green houses and Growth Tunnels including designing, procurement and installation of all system. B: Biochemistry (1986-1995) 1-M.SC Thesis (1986-1988): For partial fulfilment of Master’s degree in Botany research was performed in the department of Botany under the topic “Toxic effect of Barium in germination, early seeding growth soluble protein and peroxidase content in wheat. 2-Biochemical Analysis (1988-1995) Advanced techniques like gel electrophoresis, spectrophotometry, flame spectrophotometry, techniques for qualitative and quantitative analysis of various enzymes, proteins, nucleic acids and phenolic compounds in different tissues of in vitro Commercial projects Following commercial and contractual projects were conducted in private public partnership in this period: Three year project. 1998-2001: Production and dissemination of high quality potato tubers through tissue culture Technology. (Sponsored by Ministry of Science and Technology) One year project. 1998-2001: Commercial production of high quality turmeric seed through tissue culture technology. (Sponsored by National Foods Ltd. Karachi) Two year project: 2000-2001 14 Large scale propagation of sugarcane Mericlones developed through tissue culture technologies was done by a large industrial group i.e. Munnoo Group of industries. Three year project: 2002-2005 Production of Mericlones of sugarcane and potato and their large scale multiplication was done by Kissan Supplies Services. Symposia and meeting attended 2012 Organized and participated in International workshop entitled “Principles of Plant Breeding and advancement of molecular techniques to breeding of Bioscience” on 21st May -6th June,2012 at Biotechnology &Microbiology Department,LCWU.Lahore. 2012 Participated in short training courses for officers entitled”Accountability & Responsibility in Financial matters with special focus an Audit& Audit objections “on 4th -8th June 2012 at Management &Professional Development Department,Govt. Of Punjab,Lahore. 2012 Organized and participated in International workshop entitled “Writing winning grant proposal” on 12th June , 2012at Biotechnology &Microbiology Department,LCWU.Lahore. Participated in 2nd International Conference of Plant Scientist & 11th National Meeting of Plant Scientists organized by Pakistan Botanical Society on 22nd to 24th of Feb, 2011 at G.C University,Lahore. 2011 Participated in International Conference on Sustainable Approaches for Pest and Vector management in Pakistan ,organized by Zoological Society,Department of Zoology,LCWU ,Lahore on 21st-22nd Feb,2011. 2010 Participated in the 2nd International seminar on ‘Medicinal plants” on 14-16th Jan.2010 organized By Phytochemistry Section of Department of Chemistry, Lahore College for university, Lahore. 2010 Participated in the seminar on ‘New Trends In Chemistry” on 14-15th Jan.2010 organized By Department of Chemistry, Lahore College for university, Lahore. 2011 2009 Participated in the National Symposium on “Pharmacological studies of Medicinal Plants”on 20th June, 2009 organized by Organic section of Department of Chemistry, Lahore College for university, Lahore. 2009 Attended a special lecture on “Arsenic Pollution in Bangladesh” on 7th May, 2009 organized by environmental science department, Lahore College for university, Lahore. 2009 Participated in seminar on “Agbiotech in Pakistan----Challenges & opportunities” organized By the Lahore Chamber of Commerce and Industry, Lahore on 25th April, 2009. 2009 Participated two days workshop on operational and application of analytical instrumentation of UVspectrophotometer and PCR on 9-10th April.2009, arranged by Institution of Continuing education &extension of University of vetenary and animal sciences, Lahore. 15 2008 Three days 9th Biennial conference on Advances in Biochemistry and Molecular Biology, organized by Pakistan Society for Biochemistry and Molecular Biology in Arid Agriculture University, Rawalpindi on December 17-20,2008. 2008 Two days training and special lectures on PCR technology and DNA Sequencer, organized by Environmental Science Department, LCWU Lahore on 17-19 May, 2008. 2008 Two days 1st international seminar on medicinal plants (ISMP) organized by organic section of Chemistry Department ,Lahore college for women University, Lahore on 15-17 May,2008. 2008 Two days seminar on Human Rights, Governance and Democracy in Pakistan from March 13-14th, 2008 arranged by British Alumni Association, Pakistan. 2008 One day seminar on FTIR Spectroscopy in modern QC Laboratories & FTIR and Raman Spectroscopy in Research and Development on 8th May, 2008 by Rays Technologies. 2007 First International Conference on Recent Advances in Chemistry arranged by Department of Chemistry, Lahore College for women university, Lahore. 2007 One day seminar (21st June) on New Technology: Tools in the hands of Commercial Scientists arranged by Department of Chemistry, Lahore College for women university, Lahore. A paper was presented on Introduction of Plant Tissue Culture and its application. 2007 5th National Training Course on Modern Techniques in Biotechnology from May 28-June1, 2007arranged by National Institutes for Biotechnology and Genetic Engineering (NIBGE), Faisalabad. 2006 First International Conference of Business Opportunities in Industrial Biotechnology. Organized by Federation of Asian Biotech Association and G.C. University, Lahore. 2006 Bio forum 2006 International Conference on Industrial Biotechnology organized by CEMB, National Biotechnology Commission and Ministry of Science and Technology. 2006 One day International Conference on Fermentation Technology arranged by Department of Biochemistry and Biotechnology, Punjab University. 2004 National Conference of Sugarcane Cultivation and Improvement held at Shakarganj Sugarcane Research Institute, Jhang. 2000 Seventh National conference of plant scientist held at Department of Botany University of the Punjab, Lahore, Pakistan. 1999 Second Meeting of Potato working group to develop the national strategy y on potato seed production. 1995 Attended “Fifth National conference of Plant scientist” held at National Agriculture Research Centre NARC, Islamabad. 16 1993 Participated in “Third National conference of Plant Tissue Culture scientist” held at University of Peshawar summer camp. Baragali 1992 Participated in “International Comstech NIAB workshop on Agro-climatology, pests and diseases and their control “held at NIAB, Faisalabad. 1991 Attended the “All Pakistan Science organized by Science international, Lahore. Conference” held at Khanuspur, MEMBERSHIP OF SCIENTIFIC SOCIETIES AND OTHER ACTIVITY IN UNIVERSITY PUBLIC OR ADMINISTRATIVE AFFAIRS Member of the “Pakistan Botanical Society”. Member “Bangladesh Botanical Society”. Member “Kitchen Garden Society”. Member “Advisory Committee of Baghe-Jinnah”. Member of Academic Council of LCWU, Lahore Member, Biotechnology Committee, Lahore Chamber of Commerce, Lahore Member, Horticulture Society, Lahore. Coordinator of MS and Ph.D Program, Lahore College for Women University, Lahore Member University Purchase Committee LCWU ,Lahore Member Eligibility Committee of LCWU. Member, Senior Faculty forum, LCWU, Lahore. From 2005 to 2009 worked as coordinator in examination of Botany Department, Lahore College for Women University, Lahore. From 2005 to 2009 worked as member Purchase Committee of Botany Department, Lahore College for Women University, Lahore. 17 From 2006 to 2007 worked as Member Disciplinary Committee, of Lahore College for Women University, Lahore. Worked as Focal Person in curriculum development of MS leading PhD program in Botany Department. Appointed as Management Representative in quality assurance program (ISO 9000) in Botany Department, Lahore College for Women University, Lahore. 18