KunLiang Guan, Ph.D.
CURRICULUM VITAE
PERSONAL DATA
Kun-Liang Guan
Sanford Consortium for Regenerative Medicine, RM 4010, 2880 Torrey Pines Scenic Dr., La
Jolla, CA 92037-0695.
Tel: 858-822-7945, e-mail: kuguan@ucsd.edu
EDUCATION:
1978-1982
Undergraduate student in the Department of Biology.
Hangzhou University, China, B.S.
1982-1983
CUSBEA student, Graduate student in Shanghai Institute of Plant
Physiology, Shanghai, China
1983-1989
Graduate student in the Department of Biochemistry,
Purdue University, West Lafayette, Indiana, Ph.D. (Dec.17, 89)
(Biochemistry, Mentor: Henry Weiner)
1989-1991
Post doctoral fellow in the Department of Biochemistry, Purdue University
(Mentor, Jack Dixon)
ACADEMIC APPOINTMENTS
1992-1996
Assistant Professor, Department of Biological Chemistry, University of Michigan
1996-2000
Associate professor, Department of Biological Chemistry, University of Michigan
2000-2007
Professor, Department of Biological Chemistry, University of Michigan
2002
Visiting professor, Department of Chemistry and Biochemistry, University of
Colorado at Boulder
2003-2007
Research professor, Life Sciences Institute, University of Michigan
2003-2007
Halvor N. Christensen Collegiate Professor in Life Sciences, University of
Michigan
2005Adjunct professor, Fudan University, China
2007Adjunct professor, Department of Biological Chemistry, University of Michigan
2009Adjunct professor, Life Sciences Institute, Zhejiang University, China
2007Professor, Department of Pharmacology, Member, Moores Cancer Center,
University of California, San Diego
PATENT
DNA encoding an 18 kD cdk6 inhibitor protein, Ser. No. 08/482,069
Diagnosis and treatment of diseases arising from defects in the tuberous sclerosis
pathway, US patent 7169594 (issued January 30, 2007); Foreign patent references
WO93/19752
SCIENTIFIC ACTIVITY
Panel member of the NSF cell biology study section, 1995-1999
Editorial board member, Journal of Biological Chemistry, 1997-2002
Editorial board member, Biological Signals and Receptors. 1999-2002
Editorial board member, Experimental & Mol. Medicine, 2005-2013
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KunLiang Guan, Ph.D.
Editorial board member, JMCB, 2009Editorial board member, Cell Research, 2009NIH Study Section, Molecular Biology (Ad Hoc), 1998
NIH Study Section, CDF-3, 1999-2003
Reviewer member, National Natural Science Foundation, China, 2003-2006
Ad Hoc Reviewer for NCI Laboratory of Immunobiology, Oct.1-3, 2003; LIRR study
section, Oct. 18, 2005
Ad Hoc Reviewer for DOD, Oct 15, 2003; April, 2005
HHMI International investigator reviewer, 2011
Consultant. College for Life Sciences, Nazarbayev University, Kazakhstan, 2012Session Chair or Organizer for FASEB Summer Conference, Gordon Research
Conference, Cold Spring Harbor Phosphorylation Meeting, Ray Wu Society meeting,
Salk Institute Protein Phosphorylation Meeting, ASBMB Annual meetings, Keystone
Symposium
GRANT SUPPORT
Past
University of Michigan Geriatrics Center - Regulation of Protein Tyrosine Phosphatases
in Yeast, $25,000, [7/1/92 - 6/30/93], K. Guan, P.I.
American Cancer Society - Regulation of Protein Tyrosine Phosphorylation, 7/1/936/30/96, $100,000 for last years, [7/1/93-6/30/96], K. Guan, P.I.
University of Michigan G.I. Peptide Center - Regulation of Protein
Phosphorylation/Dephosphorylation in Growth Hormone Response, 9/1/93-8/31/95,
$60,000, [9/1/93-8/31/96], K. Guan, co-P.I.
University of Michigan Geriatrics Center - Activation of the Protein Kinase Cascade in
Mitogenic Growth Hormone Response. $25,000, [7/1/93-6/30/94], K. Guan, P.I.
Park-Davis/Warner Lambert - The Regulation of Mitogen Activated Protein Kinases in
Cellular Growth and Differentiation. $60,000, K. Guan, Co-P.I.
Taisho Excellency in Research Program, $71,000 (direct cost for the last year), 19972000, P.I.
GM51586-National Institute of Health-Protein Phosphorylation and Growth Factor
Function, $165,000 (direct cost for the last year,1994-1998), P.I.
National Institute of Health-Protein kinase in T-cell activation, $31,200 (for the last year,
1997-1998), P.I.
Parke-Davis/Warner Lambert-Function of the KSR kinase in signal transduction,
$90,000, Co-P.I.
GM89570-National Institute of Health-Regulation of protein tyrosine phosphorylation,
$123,974 (annual), 1995-1999, P.I.
RPG-98-352-American Cancer Society, The functions of Smads in TGFb signal
transduction, $80,000 (direct cost annually), P.I.
GM51586-National Institute of Health-Protein Phosphorylation and Growth Factor
Function, $193,000 (direct cost for the last year), 1998-2003, P.I.
GM62694-National Institute of Health-Signal transduction of the Ras family small
GTPases, $190,000 (direct cost for current year), 2000-2004, P.I.
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KunLiang Guan, Ph.D.
Regulation of the Raf kinase, Walther Cancer Institute, $95,000 (direct cost for current
year), 2000-2004, P.I.
R41CA101611-NIH-S6K and mTOR as targets for tuberous sclerosis. $155,222, 20032004, P.I.
Dec. 2002-Nov. 2005 NIH-Regulation and function of tuberous sclerosis complex
TSC1/TSC2 gene products in kidney diseases, (pilot grant for the nephrology center
program grant), $60,000 (direct cost for the current year for Guan), co-PI (PI,
Wiggins).
Dec. 2004-Dec. 2005 F011886-Department of Defense-Regulation of TSC1/TSC2
stability and Rheb GTP level by Herc1. $100,000, P.I.
Dec. 2005-Dec. 2006 F011886- -Department of Defense-BIG1 as a potential guanine
nucleotide exchange factor for Rheb. $100,000 (total cost), P.I.
Apr. 2003-May 2007 GM51586-National Institute of Health-Protein Phosphorylation
and Growth Factor Function, $235,000 (Annual direct cost).
June 2007-May 2008 W81XWH-07-1-0179, USAMRMC, DOD. Title: Regulation of the
mTOR pathway by a novel Rheb binding protein BNIP3, $100,000, P.I.
R01GM62694-08 (PI: Guan), 07/04-06/08, $203,860, Rheb Small GTPase signaling in
tuberous sclerosis
R01CA108941-04 (PI: Guan), 09/04-06/08, $198,644, TSC2 tumor suppressor protein in
cellular energy response.
R01CA113793-02 (PI: You) 02/08/06-1/31/11, $218,035 ($90,000 to Guan),
Chemoprevention with mTOR and farnesyltransferase inhibitors, mTOR
signaling: A novel mechanism of WNTS’s anabolic effects on bone
R01GM62694-10 (PI: Guan)
07/08-06/12
1.44 calendar months
NIH/GM
$190,000
Title: Regulation of Ras and Rheb modification by GCN2
W81XWH-09-1-0279, (PI, Guan)
Department of Defense
Title: Regulation of mTOR by nutrients
6/09-5/12
$150,000
.96 calendar months
CURRENT
R01CA108941-05 (PI: Guan)
NIH/ NCI
12/08-11/13
1.92 calendar months
$178,780
$ 229,650 ARRA Supplement
Title: TSC2 tumor suppressor protein in cellular energy response.
The main object of this project is to investigate the function of TSC2 tumor suppressor in cellular
energy starvation response, particularly how energy limitation regulating translation and
metabolism.
R01GM51586-15 (PI: Guan)
04/11-03/15
NIH/GM
$238,500
Title: Protein phosphorylation and growth factor function.
3
1.44 calendar months
KunLiang Guan, Ph.D.
The objectives of this project are to study the biochemical mechanism of protein phosphorylation
in regulation of cell growth in response to mitogenic growth factor stimulation.
R01CA132809-01 (PI: Guan)
03/08-02/13
1.92 calendar months
NIH/NCI
$ 207,500
Title: Regulation and function of the YAP transcription co-activator oncoprotein
The long term goals of this project are to determine the function and regulation of the Hippo
pathway in development of human cancers.
RB2-01547 (PI: Guan)
8/1/10-7/31/13
2.40
CIRM
$278,220
Title: The Function of YAP in embryonic stem cells.
The major goal of this project is to investigate the function of YAP transcription co-activator in
human embryonic stem, induction inducible pluripotent stem cells.
R01 DE015964-04 (PI: Wang)
4/1//10-3/31/15
.60 calendar months
NIH/NIDCR
$250,000 ($100,000 to Guan)
Title: Molecular and Genetic Determinants of invasion in HNSCC
The main goals of this project are to investigate the mechanism of tumor invasion of head and
neck squamous cell carcinoma.
Dr. C. Wang is the PI of this grant.
R01 EY022611 (PI: Guan)
09/01/12-08/31/16
2.40
NIH/NEI
$250,000
Title: Regulation of the Hippo pathway and its role in uveal melanoma
The main goal of this project is to determine the mechanism of YAP regulation by G-proteins
and elucidate the function of YAP activation in uveal melanoma, particularly those with
activating mutations in Gq and G11.
HONORS AND AWARDS
1984
Membership of The Honor Society of Agriculture,
Gamma Sigma Delta
1989
The Arnold Kent Balls Award, Department of
Biochemistry, Purdue University
1998-2003
MacArthur Fellow, The John D. Catherine T. MacArthur Foundation
1999
Schering-Plough Award (Young Investigator Award), American Society
of Biochemistry and Molecular Biology
1999
Yong Investigator Award, Society of Chinese Bioscientist Association
April 1999
Procter & Gamble Lecture, Purdue University
April 2004
Susan Swerling Lecture, Dana-Farber Cancer Institute, Harvard Medical
School
2004
Faculty Recognition Award, University of Michigan
2006
Distinguished Alumni Award, Purdue University Agriculture School
2011
AAAS fellow
4
KunLiang Guan, Ph.D.
2011
2011
2012
Ray Wu Award, CBI Society
Zhu Kezhen Distinguished Alumni Lecture, Zhejiang University, China
Keynote Lecture, Gordon Research Conference, Phosphorylation and Gprotein signaling network
MEMBERSHIP AND OFFICES IN PROFESSIONAL SOCIETIES
Ray Wu Society
American Society of Biochemistry and Molecular Biology
TEACHING ACTIVITY
1993-2007
Biochemistry 576, Signal transduction, Course co-director
1995-2001
Biochemistry 585, Cell cycle, Course director
2002-2005
Biochemistry 591, Special topics in Signal Transduction, Course co-director
2003-2007
Biochemistry 597, Critical analysis
2007Biomed 200 (guest lectures)
2008-2010
Biochemistry (for medical students)
2011
BMS 275 (co-director with Jing Yang)
Current trainee
Postdoctoral fellow
Youngchul Kim
Haixin Yan
Ryan Russell
Jenna Jewell
Faxing Yu
Joungsoon Mo
Henry Park
Carsten Hansen
Toshiro Moroishi
Graduate Students:
Karen Tumaneng
Guangbo Liu
Steven Plouffe
Past trainee
Chaofeng Zheng, Research Fellow (1992-1994), President, Biomyx
Steve Dudas, Research Fellow (1993-1995), Scientist, Henry Ford Hospital
Elizabeth Butch, Research Fellow (1993-1998), Associate Professor, Eastern Michigan
University
Toshiro Sugimoto, Research Fellow (1995-1997), Lecturer, Shiga University, Japan
Lijyun Syu, Research Fellow (1994-1996), Scientist, University of Michigan
5
KunLiang Guan, Ph.D.
Tomas Lanigan, Research Fellow (1999-2002), Director, Vector Core, University of Michigan
Bao-Hong Zhang, Research Fellow (2000-2002), Scientist, Metabasis
Yong Li, Postdoctoral fellow (2001-2006), Research investigator, University of Michigan
Eunjung Kim, Postdoctoral fellow (2004-2007), Assistant professor, Catholic University of
Daegu, Korea
Ken Inoki, Postdoctoral fellow (2002-2004), Assistant professor, University of Michigan
Hongjiao Ouyang, Postdoctoral fellow (2004-2007), Assistant professor, University of Pittsburgh
Weiquan Li, Postdoctoral fellow (1999-2006) Research investigator, University of Michigan
Hiroyuki Mori, Postdoctoral fellow (2005-2007), Postdoctoral fellow, University of Michigan
Xiaoming Zhou, Postdoctoral fellow (2005-2007), Research investigator, University of Michigan
Tsuneo Ikenoue, Postdoctoral fellow (2004-2007), Assistant professor, University of Tokyo
Young Kang, Research associate (2007-2008), Assistant professor, Scripps Research Institute
Noelia Valbuena, Postdoctoral fellow (2008-2009), Salamaca University, Spain
Wei Wang Postdoctoral fellow (2008-2010), Postdoctoral fellow, Burnham Institute
Amy Lin, Postdoctoral fellow (2008-2010)
Hideki Okazawa, Postdoctoral fellow (2009-2011), Assistant professor, Kobe University, Japan
Joungmok Kim, Postdoctoral fellow (2007-2012), Assistant Professor, Kyung Hee University,
Korea
Jack Zhao, Postdoctoral fellow (2009-2011), Assistant project scientist, UCSD
Xiaoduo Xie, Postdoctoral fellow (2008-2011), Scientist, Beigen
Bin Zhao, Postdoctoral fellow (2009-2012), Assistant professor, Zhejiang University, China
Ian Lian, Postdoctoral fellow (2007-2012)
Seong Noh, MD/Ph.D. Student, 2001, Fellow, University of Washington
Scott Stewart, Ph.D. Student, 2000, Research associate, the Salk Institute
Xiaoli Zhan, Ph.D. Student, 2000, Postdoctoral fellow, UCLA
Eric Tang, MD/Ph.D. student, 2002, Postdoctoral fellow, UCLA
Haris Vikis, Ph.D. Student, 2003, Assistant Research scientist, Washington University
Huira Chong, Ph.D. Graduate student (2005), Postdoctoral fellow, University of Michigan
Jeeyong Lee, Ph.D. Student 2005, Postdoctoral fellow, Yongsei University, Korea
Jennifer Aurandt, Ph.D. Student, 2005, Assistant Professor, Kettering University
Robert Kruger, Ph.D. Student, 2005, Editor, Cell
Mike Corradetti, MD/Ph.D. Student, 2006, Fellow, UPenn Medical School
Qian Yang, Ph.D. Student, 2007, Postdoctoral fellow, University of Michigan
Joe Lee, MD/Ph.D. Student, 2007, Columbia U
Bin Zhao, Ph.D. Student, 2009, Zhejiang University
Minkan Lu, Ph.D. Student (Zhejiang University) 2011
Li Li, Ph.D. Student 2011, Sanford Burnham Institute
Rui Gong, Ph.D. Visiting student
Thesis Committee
1993, M. Hull, Ph.D.
1994 K. Quadnt, Ph.D.
1995 M. Szczypka, Ph.D.
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KunLiang Guan, Ph.D.
1997 Jirundon Yuvaniyama, Ph.D. , Hangjong Duan, Ph.D.; Deb Meyers, Ph.D.
1998 Larry Adams, Ph.D.; Mary Stefa, Ph.D. ; J. Clemens, Ph.D. ; C. Zimmerman, Ph.D. ;
Witoon Titrasophon, Ph.D. ; Seong Noh, Ph.D. ; Sean Collin, Ph.D.
2000 Scott Stewart, Ph.D.; X. Liu, Ph.D.; Matt Wishart, Ph.D. 2000
2001 Sarah Ross, Ph.D.; John Stansberry, Ph.D.
2002 Steven Juris, Ph.D.; Eric Tang, Ph.D.
2003 Gary Meyer, Ph.D.; Claudia Fugerson, Ph.D.; Chuanyun Liu, Ph.D.; Timothy
Blauwkamp, Ph.D.; Xiaohua Shen, Ph.D.; Mike Roth, Ph.D.; Haris Vikis, Ph.D.; Sunny Lee,
Ph.D.; David Karnak, Ph.D.
2004 Huira Chong, Ph.D.
2005 Peng Peng, Ph.D.; Jeeyong Lee, Ph.D.; Stephen Carey, Ph.D.; Jason Kurzer, Ph.D.;
Mattabhorn Phimphilai, Ph.D.
2006 Kunyoo Shin, Ph.D., Mike Corradetti, Ph.D., Brian Cha, Ph.D; Ifan Lodi.Ph.D
2007 Qiang Wang, Ph.D.; Matthew Veldam, Ph.D.; Matthew Pratt-Hyatt, Ph.D.
2009 Bin Zhao, Ph.D.
2011 Emi Casas, Ph.D.; Li Li, Ph.D.; Noel Warfel, Ph.D.
2012 Audrey O’Neal, Ph.D., Marina Kolesnichenko, Ph.D. (Oxford University)
INVITED PRESENTATIONS (2010-)
7th Annual Cancer Drugs Research and Development Conference, La Jolla, Jan. 2010
University of California at San Diego, Department of Medicine, Jan. 2010
15th Aichi International Cancer Conference, Nagoya, Japan, March 2010
14th International Congress of Endocrinology, Kyoto, Japan, March 2010
LAM Conference, Cincinnati, Apr. 2010
Einstein College of Medicine, Bronx, Apr. 2010
SCBA Houston Chapter Symposium, Houston, May, 2010
Han Yang University, Seoul, Korea, May, 2010
Symposium of System Biology for Biomedical Application, Seoul, Korea, May, 2010
Nanjing University, Nanjing, China, May 2010
International Symposium on Neuro-Signaling, Wuhan, China, May, 2010
Neurooncology, UCSD, June, 2010
Cancer Biology, UCSD, June, 2010
Forth Military Medical University, July, 2010
Peking Union Medical College, July, 2010
University of California at Berkeley, Sep. 2010
FASEB Summer conference on AMPK, Kyoto, Japan, Oct. 2010
Xianshan Conference on Proteomics, Beijing, China, Oct. 2010
Department of pathology, UCSD, La Jolla, Oct. 2010
The Second workshop on the Hippo pathway, Rome, Italy, Nov. 2010
Cancer Metabolism Conference, IRB, Barcelona, Spain, Nov. 2010
mTOR Signaling in Health and Disease, Biochemical Society, London, UK, Nov. 2010
Zhejiang University, Hangzhou, China, Dec. 2010
Duke University, Department of Pharmacology and Cancer Biology, Jan. 2011
University of Nebraska Medical Center, March 2011
Shanghai Institute of Biochemistry and Cell Biology, China, March 2011
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KunLiang Guan, Ph.D.
Institute of Molecular and Cell Biology, Singapore, March 2011
AACR 102nd Annual Meeting, Orlando, April 2011
Northwestern University, Chicago, April 2011
36th Lineberger Symposium on Cancer Metabolism, UNC, Chapel Hill, April 2011
GSK Pharmaceuticals. Collegeville, April 2011
TRCP symposium, Yonsei University, Seoul, Korea, May 2011
Korean Society for Biochemistry and Molecular Biology Annual Meeting, Seoul, May 2011
Wuxi Pharm, Shanghai, China, May 2011
Agios Pharmaceutical, Cambridge, MA, May 2011
Cold Spring Harbor Symposium, Metabolism & Disease, June 2011
FASEB Summer conference on acetylation, Steamboat, June 2011
Molecular Therapeutics of Cancer conference, Pacific Grove, July 2011
FASEB summer conference on protein phosphorylation, Snowmass, July, 2011
Xiamen University, Xiamen, China, July 2011
SCBA conference, Guangzhou, China, July 2011
Guangzhou Institutes of Biomedicine and Health, July 2011
CBI Society Biannual Conference, China, Aug. 2011
FASEB Summer Conference, Nutrient Control of metabolism & Cell Signaling, Steamboat,
Aug. 2011
Ontario Institute for Cancer Research, Toronto, Sep. 2011
Genentech, San Francisco, Sep. 2011
RIKEN-Cell Press Symposium, Kobe, Japan, Oct. 2011
MD Anderson Cancer Center, Houston, Oct. 2011
Mayo Clinic, Rochester, Oct. 2011
University of Illinois at Chicago, Nov. 2011
Nazarbayev University, Astana, Kazakhstan, Nov. 2011
Zhejiang University, Hangzhou, China, Nov. 2011
Tsinghua University, Beijing, China, Nov. 2011
Peking University, Beijing, China, Nov. 2011
Zing conference on Autophagy, Cancun Mexico, Dec. 2011
Institute of Molecular Biology, Academia Sinica, Taiwan, Jan. 2012
Scripps Research Institute, La Jolla, Jan, 2012
Oregon Health Sciences University, Portland, Feb. 2012
University of Utah, Salt Lake City, Feb. 2012
Keystone Symposium on Cancer Metabolism, Banff, Canada, Feb. 2012
AACR Annual meeting, Chicago, Apr. 2012
John Hopkins Medical School, Apr. 2012
6th AUHUPO Annual Meeting, Beijing, May, 2012
Ben May Symposium, Cancer Metabolism, University of Chicago, May, 2012
Growth regulation by the TOR pathway, Les Treiles, Nice, France, June, 2012
Phosphorylation & G-protein signaling, Gordon Research Conference, June 2012
Novartis, Shanghai, China, July, 2012
Post-translational regulation of cell signaling, Salk Institute, La Jolla, Aug. 2012
Cancer Metabolism symposium, Sanford Burnham Institute, La Jolla, Aug. 2012
Roswell Park Cancer Institute, Buffalo, Sep. 2012
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KunLiang Guan, Ph.D.
Beth Israel Deaconess Medical Center, Boston, Sep. 2012
Cell Signaling Technology, Salem, Sep. 2012
University of Wisconsin, Madison, Sep. 2012
Memorial Sloan-Kettering Cancer Center, New York, Sep. 2012
University of Alabama at Birmingham, Birmingham, Oct. 2012
Fourth International Stem cell Symposium, City of Hope National Medical Center, Duarte,
Oct. 2012
Friedrich Miescher Institute, Basel, Oct. 2012
2012 Louis-Jeantet Symposium, Geneva, Oct. 2012
Pfizer Inc., La Jolla, Nov. 2012
Washington University, St. Louis, Jan. 2013
University of Southern California, Feb. 2013
AACR Annual Meeting, Washington DC, Apr. 2013
COMMITTEE AND ADMINISTRATIVE SERVICE
1994-03 Department Graduate Admission Committee (co-chair of the department
recruiting committee)
1996-2001 Review Committee for Rackham Graduate School
1997-07 Student Award Committee
1998-03 PIBS admission committee
2002-07 MDRTC Grant Advisory Council
2002-07 MSTP Operating Committee
2003-06 Biomedical Research Council, member
2003
Faculty search committee chair, Biological Chemistry Department
2004
Faculty search committee chair, Life Sciences Institute
2008Department space committee
2008Co-leader, Biology Program, Moores Cancer Center
2012Executive Committee, Cancer Therapeutic Training Program
2012
Faculty Search Committee
BIBLIOGRAPHY
Peer reviewed research papers
1.
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Farres, J., Guan, K-L., and Weiner, H. (1988) Signal peptide sequence of rat
mitochondrial aldehyde dehydrogenase deduced from cDNA. Biochem. Biophys. Res.
Comm. 150, 1083-1087
Guan, K-L., Pak, Y.K., Tu, G.C., Cao, Q.N., and Weiner, H. (1988) Purification and
characterization of beef and pig liver aldehyde dehydrogenases. Alcoholism: Clin. Exp.
Res. 12, 713-719
Farres, J., Guan, K-L., and Weiner, H. (1989) Primary structures of rat and bovine liver
mitochondrial aldehyde dehydrogenase deduced from cDNA sequences. Eur. J. Biochem.
180, 67-74
Guan, K-L., and Weiner, H. (1989) Influence of the 5’-end region of aldehyde
dehydrogenase mRNA on translational efficiency: potential secondary structure inhibition
of translation in vitro. J. Biol. Chem. 264, 17764-17769.
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Guan, K-L., and Weiner, H. (1990) Sequence of the precursor of bovine liver
mitochondrial aldehyde dehydrogenase as determined from its cDNA and its gene and its
functionality. Arch. Biophys. Biochem. 277, 351-360.
Guan, K-L., Haun, R.S., Watson, S.J., Geahlen, R.L., and Dixon, J.E. (1990) Cloning and
expression of a protein-tyrosine-phosphatase. Proc. Natl. Acad. Sci. USA 87, 1501-1505.
Guan, K-L. and Dixon, J.E. (1990) Protein tyrosine phosphatase activity of an essential
virulence determinant in Yersinia. Science 249, 553-556.
Guan, K-L., and Dixon, J.E. (1991) One step purification of eukaryotic proteins
expressed in E. coli: An improved thrombin cleavage and purification procedure of fusion
proteins with glutathione-S-transferase. Anal. Biochem. 192, 262-267.
Bliska, J.B., Guan, K-L., Dixon, J.E., and Falkow, S.A. (1991) Tyrosine phosphate
hydrolysis of proteins by an essential Yersinia virulence determinant. Proc. Natl. Acad.
Sci. USA 88, 1187-1191.
Guan, K-L., Broyles, S.S., and Dixon, J.E. (1991) Vaccinia virus encodes a Tyr/Ser
protein phosphatase. Nature 350, 359-362.
Guan, K-L., Deschenes, R.J., Qiu, H., Dixon, J.E. (1991) Cloning and expression of a
yeast protein tyrosine phosphatase. J. Biol. Chem. 266, 12964-12970.
Guan, K-L. and Dixon, J.E. (1991) Evidence for protein tyrosine phosphatase catalysis
proceeding Via a cysteine-phosphate intermediate. J. Biol. Chem. 266, 17026-17030.
(Named JBC classic paper, JBC 281, e42, 2006).
Ni, L.-Y., Guan, K-L., Zalkin, H., and Dixon, J.E. (1991) De novo purine nucleotide
biosynthesis: cloning and expression of a eukaryotic cDNA encoding 5-aminoimidazole4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase. Gene 106, 197-205.
Guan, K-L., Deschenes, R.J., and Dixon, J.E. (1992) Isolation and characterization of a
second protein tyrosine phosphatase gene, PTP2, from Saccharomyces cerevisiae. J. Biol.
Chem. 267, 10024-10020
Guan, K-L., Hakes, D., Wang, Y., Park, H.D., Cooper, T.G., and Dixon, J.E. (1992) A
novel yeast protein phosphatase related to the vaccinia virus VH1 phosphatase is induced
by nitrogen starvation. Proc. Natl. Acad. Sci. USA, 89, 12175-12179.
Millar, J.B.A., Russell, P., Dixon, J.E., and Guan, K-L. (1992) Negative regulation of
mitosis by functionally overlapping PTPases in fission yeast. The EMBO J., 11, 49434952.
Guan, K-L., Hakes, D., Park, H.D., Cooper, T.G., and Dixon, J.E. (1993). A eukaryotic
homolog to viral Ser/Thr/Tyr phosphatase. Treds in Biochem. Sci. 18, 6.
Zheng, C.F. and Guan, K-L. (1993) Cloning and characterization of two distinct human
ERK activator kinases, MEK1 and MEK2, J. Biol. Chem. 268, 11435-11439.
Zheng, C.F. and Guan, K-L. (1993) Dephosphorylation and inactivation of the mitogen
activated protein kinases by a mitogen induced protein phosphatase. J. Biol. Chem. 268,
16116-16119.
Guan, K-L., Farh, L., Marshall, T.K., and Deschenes, R.J. (1993) Normal mitochondrial
structure and genome maintenance in yeast requires the dynamine like product of the
MGM1 gene. Current Genetics 24, 141-148.
Zheng, C.F. and Guan, K-L. (1993) Properties of MEKs, the kinases that phosphorylate
and activate the extracellular signal-regulated kinases. J. Biol. Chem. 268, 23933-23939.
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Zheng, C.F. and Guan, K-L. (1994) Activation of MEK family kinases requires
phosphorylation of two conserved Ser/Thr residues. The EMBO J. 13, 1123-1131.
Zheng, C.F., Ohmichi, M., Saltiel, A.R., and Guan, K-L. (1994) Growth factor induced
MEK activation is primarily mediated by an activator different from c-raf. Biochemistry,
33, 5595-5599.
Zheng, C.F. and Guan, K-L. (1994) Cytoplasmic localization of the mitogen
activated protein kinase activator, MEK. J. Biol. Chem. 269, 19947-19952.
Frost, J.A., Alberts, A.S., Sontag, E., Guan, K-L., Mumby, M.C., and Feraminsco, J.R.
(1994) Simian virus 40 small T antigen cooperates with mitogen activated kinases to
stimulate AP-1 activity. Mol. Cell. Biol. 14, 6244-6252.
Guan, K-L., Jenkins, C.W., Li, Y., Nichols, M.A., Wu, X., Matera, G., and Xiong, Y.
(1994) Growth suppression by p18, a novel p16INK4/MTS1 and p14INK/MTS2-related
CDK6 inhibitor, correlates with wild type pRb function. Genes & Dev. 8, 2939-2952.
Guan, K-L. and Butch, E. (1995) Isolation and characterization of a novel dual specific
phosphatase, HVH2, which selectively dephosphorylates the mitogen activated protein
kinase. J. Biol. Chem. 270, 7197-7203.
Pang, R., Zheng, C.-F., Guan, K-L., and Saltiel, A.R. (1995) Nerve growth factor
stimulates a novel protein kinase in PC-12 cells that phosphorylates and activates MEK.
Biochem. J. 307, 513-519.
Pham, C.D., Arlinghaus, R.B., Zheng, C.-F., Guan, K-L., and Singh, B. (1995)
Characterization of MEK1 phosphorylation by v-Mos protein. Oncogene 10, 1683-1688.
Wu, Y., Han, M. and Guan, K-L. (1995) Mek-2, a Caenorhabditis elegans MAP kinase
kinase, functions in Ras-mediated vulval induction and other development events. Genes
& Dev. 9, 742-755.
Kornfeld, K., Guan, K-L., and Horvitz, H.R. (1995) The C. elegans mek-2 is required for
vulval induction and encodes a protein similar to the protein kinase MEK. Genes & Dev.
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Rui, D., Zheng, C.-F., Guan, K-L., and Williams, J. A. (1995) Activation of MAP kinase
kinase (MEK) and Ras by cholecystokinin in rat pancreatic acini. Am. J. Physiol. 268,
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Wang, Q.M, Guan, K-L., Roach, P.J., and Depaoli-Roach, A.A. (1995) Phosphorylation
and activation of the ATP-Mg-Dependent Protein Phosphatase by the Mitogen Activated
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Zhang, Z. Y., Zhou, G, Denu, J. M., Wu, L., Tang, X., Mondesert, O., Russell, P., Butch,
E., and Guan, K-L. (1995) Purification and characterization of the low molecular weight
protein tyrosine phosphatase, Stp1, from fission yeast S. pombe. Biochemistry 34, 1056010568.
Chaurch, D.L., Guan, K-L. and Lambie, E.J. (1995) mek-2, mpk-1/sur-1 and let-60 ras
are required for meiotic cell cycle progression in C. elegans. Development. 121, 25252535.
Waters, S. B., Holt, K.H., Ross, S.E., Syu, L.J., Guan, K-L., Saltiel, A.R., Koretzky,
G.A., and Pessin, J.E. (1995) Desensitization of Ras activation by a feedback
disassociation of the SOS-Grb2 complex. J. Biol. Chem. 270, 20883-20886.
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T., Kashiwagi, S., Guan, K-L., and Kikkawa, R. (1996) Atrial natriuretic peptide induces
the expression of MKP-1, a mitogen activated protein kinase phosphatase, in glomerular
mesangial cells. J. Biol. Chem. 271, 544-547.
Wu, X., Noh, S., Zhou, G., Dixon, J.E., Guan, K-L. (1996) Selective activation of MEK-1
but not MEK-2 by A-raf from epidermal growth factor stimulated Hela cells. J. Biol.
Chem. 271, 3265-3271.
Butch, E. and Guan, K-L. (1996) Characterization of ERK1 activation site mutants and
the effect on recognition by MEK1 and EMK2. J. Biol. Chem. 4230-4235.
Robinson, M.J., Cheng, M., Khokhlatchev, A., Ebert, D., Ahn, N., Guan, K-L., Stein,
B., Goldsmith, E., and Cobb, M.H. (1996) Contributions of the mitogen-activated protein
(MAP) kinase backbone and phosphorylation loop to MEK specificity.: J Biol Chem 271,
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Gupta, A.K., Lee, Y.L., Galoforo, S.S., Berns, C.M., Martinez, A.A., Corry, P.M., Wu, X.,
and Guan, K-L. (1997)Differential effect of glucose deprivation on MAPK activation in
drug sensitive human breast carcinoma MCF-7 and multidrug resistant MCF-7/ADR cells.
Mol. Cell. Biochem. 170, 23-30.
Gupta, A. K., Galoforo, S. S., Berns, C. M., Martinez, A. A., Corry, P. M., Guan, KL., and Lee, Y. J. (1996) Elevated levels of ERK2 in human breast carcinoma MCF-7
cells transfected with protein kinase C. Cell Prolif. 29, 655-663.
Jiang, Y., Li, Z., Scharz, E.M., Guan, K-L., Ulevitch, R.J., and Han, J. (1997) Structurefunction studies of p38 MAP kinase: loop12 influences substrate specificity and
autophosphorylation but not upstream kinase selection. J. Biol. Chem. 272, 11096-1102.
Gorgas, G., Butch, E., Guan, K-L., and Miller, R.A. (1997) Diminished activation of the
MAP kinase Pathway in CD3-stimulated T lymphocytes from old mice. Mechanism of
Ageing and Dev. 94, 71-83.
Zhan, X., Deschenes, R.J., and Guan, K-L. (1997) Differential regulation of FUS3 MAP
kinase by tryosine specific phosphatases PTP2/PTP3 and dual specificity phosphatase
MSG5 in S. cerevisiae. Genes & Dev. 11, 1690-1702.
Syu, L-J., Guan, K-L., and Saltiel, A. R. (1997) Inducible expression of a mutant form
of MEK1 in Swiss3T3 cells. J. Cell. Biochem. 67, 367-377.
Dabrowski, A., Groblewski, G. E., Schaefer, C., Guan, K.-L., and Williams, J.A. (1997)
Cholecystokinin activates a MAP kinase cascade by multiple mechanisms in rat acinar
cells. Am. J. Physiol. 273, C1472-1479.
Tang, D.G., Guan, K-L., Li, L., Honn, K.V., Chen, Y.Q., Rice, R.L., Taylor, J.D., and
Porter, A.T. (1997) Suppression of W256 carcinosarcoma cell apoptosis by arachidonic
acid and other polyunsaturated fatty acids. Int. J. Cancer 72, 1078-1087.
VanderKuur, J. A., Butch, E., Waters, S.B., Pessin, J. E., Guan, K.-L., and Carter-Su, C.
(1997) Signaling molecules involved in coupling growth hormone receptor to MAP kinase
activation. Endocrinology 138, 4301-4307.
Sugimoto, T., Scott, S., and Guan, K.-L. (1997) The calcium/calmodulin dependent
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Zhao, Y., Wu, L., Noh, S. J Guan, K.-L., and Zhang, Z-Y. (1998) Altering the
nucleophile specificity of a protein-tyrosine phosphatase catalyzed reaction: probing the
function of the invariant glutamine residues. J. Biol. Chem. 273, 5484-5492.
Sugimoto, T., Scott, S., and Guan, K.-L. (1998) The kinase suppressor of Ras (KSR)
modulates growth factor and Ras signaling by uncoupling Elk-1 phosphorylation from
MAP kinase activation. The EMBO J. 17, 1717-1727.
Goldman, D., Sapru, M.K., Stewart, S., Plotkin, J., Libermann, T.A., Wasylyk, B., and
Guan, K.-L. (1998) Cloning and characterization of GETS-1, a goldfish Ets family
member that functions as a transcriptional repressor in muscle. Biochem. J. 335, 267-275.
Hodge C, Liao JF, Stofega M, Guan, K.-L., Carter-Su C, Schwartz J (1998) Growth
hormone stimulates phosphorylation and activation of Elk-1 and expression of c-fos, egr1, and junB through activation of extracellular signal-regulated kinases 1 and 2. J. Biol.
Chem. 273, 31327-31336.
Noh, S.J., Li, Y., Xiong, Y., and Guan, K.-L. (1999) Identification of functional elements
of p18INK4C essential for binding and inhibition of cyclin-dependent kinase (CDK)4 and
CDK6. Cancer Research 59, 558-564.
Tan, M., Heizmann, C.W., Guan, K-L., Schafer, B.W., & Sun, Y. (1999) Transcriptional
activation of the human S100A2 promoter by wild type p53. FEBS Lett. 445, 265-268.
Tan, M., Li, S., Swaroop, M., Guan, K-L., Oberley, L.W., & Sun, Y. (1999)
Transcriptional activation of the human glutathione peroxidase promoter by p53. J. Biol.
Chem. 274, 12061-12066.
Tang, E., Nunez, G., Bar, D., and Guan, K.-L. (1999) Negative regulation of the forkhead
transcription factor FKHR by AKT. J. Biol. Chem. 274, 16741-16746.
Stewart, S., Sundaram, M., Zhang, Y., Lee, J., Han, M., and Guan, K.-L. (1999) Kinase
Suppressor of Ras (KSR) forms a multi-protein signaling complex and modulates MEK
localization. Mol. Cell. Biol. 19, 5523-5534.
Herzog, C. R., Noh, S., Lantry, L.E., Guan, K.-L., and You, M. (1999) Cdkn2a encodes
functional variation of p16ink4a but not p19ARF, which confers selection in mouse lung
tumorigenesis. Mol. Carcinogenesis 25, 92-98.
Zhan, X. L., and Guan, K.-L. (1999) Specific protein-protein interaction accounts for the
in vivo substrate selectivity of Ptp3 towards the Fus3 MAP kinase. Genes &
Development. 13, 2811-2817.
Xiao G. Jiang D. Thomas P. Benson MD. Guan K.-L. Karsenty G. Franceschi RT. (2000)
MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor,
Cbfa1. Journal of Biological Chemistry. 275, 4453-4459.
Tan, M., Wang, Y., Guan, K-L., and Su Y. (2000) PTGF, a novel TGF- superfamily
member, is a p53 target gene that mediates p53-induced growth suppression. Proc. Natl.
Acad. Sci. USA 97, 109-114.
Zhan, X., Hong, Y., Zhu, T., Mitchell, A.P., Deschenes, R.J., and Guan, K.-L. (2000)
Essential role of tyrosine phosphorylation of the RIM11 kinase in meiosis and sporulation
of Saccharomyces cerevisiae. Mol. Biol. Cell. 11, 663-676.
Stewart, S. and Guan, K.-L. (2000) The dominant negative Ras mutant, N17Ras, can
inhibit signaling independently of Ras activation. J. Biol. Chem. 275, 8854-8862.
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Li, W., Han, M., and Guan, K.-L. (2000) The leucine-rich repeat protein Sur-8 enhances
MAP kinase activation and forms a complex with Ras and Raf. Genes & Development,
14, 895-900.
Guan, K.-L., Figueroa, C., Brtva, T., Zhu, T., Taylor, J., Barber, T., and Vojtek, A.
(2000) Negative regulation of the serine/threonine kinase B-Raf by Akt. J. Biol. Chem.
275, 27354-27359.
Simon, A. R., Vikis, H.G., Fanburg, B.L., Stewart, S., Cochran, B.H., and Guan, K.-L.
(2000) Regulation of STAT3 by direct binding to the small G protein Rac1. Science 290,
144-147.
Koga, M., Zwaal, R., Guan, K-L., Avery, L., and Ohshima, Y. (2000) A MAP kinase
kinase MEK-1 in C. elegans is involved in stress responses. EMBO J. 19, 5148-5156.
Zhang, B. H. and Guan, K-L. (2000) Activation of B-Raf kinase requires
phosphorylation of the conserved residues Thr598 and Ser601. EMBO J. 19, 5429-5439.
Vikis H.G., Li, W, He, Z., and Guan, K.-L. (2000) The semaphorin receptor plexin-B1
specifically interacts with active Rac in a ligand dependent manner. Proc. Natl. Acad. Sci.
97, 12457-12462.
Li, Y., Li, M., Xing, G., Hu, Z., Wang, Q., Dong, C., Wei, H., Fan, G., Chen, J., Yang,
X., Zhao, S., Chen, H., Guan, K-L., Wu, C., Zhang, C., and He, F.C. (2000) Stimulation
of the Mitogen-activated Protein Kinase Cascade and Tyrosine Phosphorylation of the
Epidermal Growth Factor Receptor by Hepatopoietin . J. Biol. Chem. 275, 37443-37447.
Tan, M., Bian, J., Guan K-L., and Sun, Y. (2001) p53CP is p51/p63, the third member of
the p53 gene family: partial purification and characterization. Carcinogenesis. 22, 295300.
Chong, H., Lee, J., and Guan, K.-L. (2001) Positive and negative regulation of Raf
kinase activity and function by phosphorylation. EMBO J. 20: 3716-3727.
Zhang, Z., Wang, Y., Vikis, H. G., Anderson, M.W., Sills, R.C., Holliday, W., Devereux,
T.R., Guan, K-L., and You, M. (2001) Wild type ras can inhibit lung carcinogenesis in
mice. Nature Genetics 29, 25-33.
Zhang, B.H., Tang, E., Zhu, T., Greenberg, M., Vojtek, A. and Guan, K-L. (2001) Serum
and glucocorticoid-inducible kinase SGK phosphorylates and negatively regulates B-Raf.
J. Biol. Chem. 276, 31620-31626.
Li, W., Chong, H., and Guan, K-L. (2001) Function of the Rho family GTPases in Ras
stimulated Raf activation. J. Biol. Chem. 276, 34728-37.
Chen, S., Guttridge, D., Tang, E., Shi, S.T., Guan, K-L., and Wang, C.Y. (2001)
Suppression of tumor necrosis factor-mediated apoptosis by NF-kB-independent
BMP/Smad signaling. J. Biol. Chem. 276, 39259-39263.
Vikis, H.G., Stewart, S., and Guan, K-L. (2002) SmgGDS displays differential binding
and exchange activity towards different Ras isoforms. Oncogene 21, 2425-32.
You Z, Saims D, Chen S, Zhang Z, Guttridge DC, Guan, K-L, MacDougald OA, Brown
AM, Evan G, Kitajewski J, Wang CY. (2002) Wnt signaling promotes oncogenic
transformation by inhibiting c-Myc-induced apoptosis. J. Cell Biol. 157, 429-40.
Vikis H.G., Li, W, He, Z., and Guan, K-L. (2002) The Plexin-B1/Rac interaction inhibits
PAK activation and enhances Sema4D ligand binding. Genes & Development. 16, 836845.
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Lu C, Li Y, Zhao Y, Xing G, Tang F, Wang Q, Sun Y, Wei H, Yang X, Wu C, Chen J,
Guan, K-L, Zhang C, Chen H, He F. (2002) Intracrine hepatopoietin potentiates AP-1
activity through JAB1 independent of MAPK pathway. FASEB J. 16, 90-2.
Inoki, K., Li, Y., Zhu, T., Wu, J. and Guan, K-L. (2002) TSC2 is phosphorylated and
inhibited by AKT and suppresses mTOR signaling. Nature Cell Biol. 4, 648-657.
(Selected by Nature Cell Biology as one of the landmark papers).
Aurandt, J., Vikis, H.G., Gutkind, S., Ahn, N., and Guan, K-L. (2002) The semaphorin
receptor plexin-B1 signals through a direct interaction with the Rho specific nucleotide
exchange factor, LARG. Proc. Natl. Acad. Sci. USA 99, 12085-12090.
Li, Y., Inoki, K., Yeung, R. and Guan, K-L. (2002) Regulation of TSC2 by 14-3-3
binding. J. Biol. Chem. 277,44593-44596.
Li, Y., Inoki, K., Vacrasis, P., and Guan, K-L. (2003) The p38 and MK2 kinase cascade
phosphorylates tuberin, the tuberous sclerosis 2 (TSC2) gene product, and enhances its
interaction with 14-3-3. J. Biol. Chem. 278, 13663-13671.
Hurley, J.H., Szhang, S., Bye, L., Marshall, M.S., DePaoli-Roach, A., Guan, K-L., Fox,
A.P. and Yu, L. (2003). Insulin Signaling Inhibits the 5-HT2C Receptor in Choroid
Plexus via MAP kinase. BMC Neuroscience, 4, 10.
Yang F, Tang E, Guan, K-L., Wang CY. (2003) IKKbeta Plays an Essential Role in the
Phosphorylation of RelA/p65 on Serine 536 Induced by Lipopolysaccharide. J. Immunol.
170, 5630-5635.
Inoki, K., Li, Y., Xu, T., and Guan, K-L. (2003) Rheb GTPase is a direct target of TSC2
GAP activity and regulates mTOR signaling. Genes & Development, 17, 1829-1834.
Lanigan, T., Liu, A., Huang, Y.Z., Mei, L., Margolis, B., and Guan, K-L. (2003) Human
Homologue of Drosophila CNK interacts with Ras effector proteins, Raf and Rlf. FASEB
J. 17, 2048-60.
Tang, E.D., Wang, C., Xiong, Y., and Guan, K-L. (2003) A role for NEMO/IKK
ubiquitination in activation of IB kinase complex by TNF. J. Biol. Chem. 278, 37297305.
Tang, E.D., Inohara, N., Wang, C., Nunez, G., and Guan, K-L. (2003) Roles of
homotypic interaction and transautophosphorylation in IKK activation. J.Biol. Chem.
278, 38566-70.
Zhang, Z., Futamura, M., Vikis, H., Wang, M., Li, J., Wang, Y., Guan, K-L., and You.,
M. (2003). Positional cloning of the major quantitative trait locus underlying lung tumor
susceptibility in mice. PNAS, 100, 12642-47.
Chong, H. and Guan, K-L. (2003) Regulation of Raf through phosphorylation and Nterminus-C-terminus interaction. J. Biol. Chem. 278, 36269-76.
Zhou, Q., Rammohan, K., Lin, S., Robinson, N., Li, Q., Liu, X., Bai, X., Yin, L.,
Scarberry, B., Du, P., You, M., Guan, K-L., Zheng. P., and Liu, Y. (2003) CD24 is a
genetic modifier for risk and progression of multiple sclerosis. Proc. Natl. Acad. Sci. 100,
15041-15046.
Inoki, K., Zhu, T., and Guan, K-L. (2003) TSC2 mediates cellular energy response to
control cell growth and survival. Cell 115, 577-590.
Ruan, Q., Xie, S., Fang, Y., Darzynkiewicz, Z., Guan, K-L., Uniyal, M. J., Dai, W.
(2004) Polo-like kinase 3 (Plk3) is Golgi-localized and involved in regulating Golgi
fragmentation during the cell cycle. Experimental Cell Res. 294, 51-59.
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KunLiang Guan, Ph.D.
99.
Yoder, J.H., Chong, H., Guan, K-L., and Han, M. (2004) Attenuation of the Ras/MAPK
signal transduction pathway via zinc ion mediated inhibition of a convergent regulatory
input. EMBO J. 23, 111-119.
100.
Yang, F,, Yamashita, J., Tang, E., Wang, H.L., Guan, K-L., Wang, C.Y. (2004)
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
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Zinc Finger Mutation C417R of I-kappaB Kinase gamma Impairs Lipopolysaccharideand TNF-Mediated NF-kappaB Activation through Inhibiting Phosphorylation of the IkappaB Kinase beta Activation Loop. J Immunol. 172, 2446-2452.
Wang, M., Devereux, T., Vikis, H., McCulloch, S., Holiday, W., Anna. C., Wang, Y.,
Debenek, Kunkel, T., Guan, K-L., and You, M. (2004) Poliota is a candidate for the
mouse pulmonary adenoma resistance 2 locus, a major modifier of chemically induced
lung neoplasia. Cancer Res. 64, 1924-1931.
Xie, S., Wang, Q., Ruan, Q., Liu, T., Jhanwar-Uniyal, M., Guan, K-L., and Dai, W.
(2004) MEK1-induced Golgi dynamics during cell cycle progression is partly mediated
by Polo-like kinase-3. Oncogene 6, 3822-9.
Li., W. and Guan, K-L. (2004) The Down syndrome cell adhesion molecule DSCAM
interacts with and activates PAK. J. Biol. Chem. 279, 32824-31.
Corradetti, M., Inoki, K., Bardeesy, N., DePinho R., and Guan, K-L. (2004) Regulation
of the TSC pathway by LKB1: Evidence of a molecular link between Tuberous Sclerosis
Complex and Peutz-Jeghers Syndrome. Genes & Dev. 18, 1533-1538.
Li, W., Lee, J., Vikis, H., et al, and Guan, K-L. (2004) Activation of FAK and Src are
receptor proximal events required for netrin signaling. Nature Neuroscience. 7, 12131221.
Basile. J., Barac, A., Zhu, T., Guan, K-L., and J. Silvio Gutkind. (2004) Class IV
Semaphorins Promote Angiogenesis by Stimulating Rho-Initiated Pathways through
Plexin-B. Cancer Research, 64, 5212-24.
Li, Y., Inoki, K., and Guan, K-L. (2004) Biochemical and functional characterization of
small GTPase Rheb and TSC2 GAP activity. Mol. Cell. Biol. 24, 7965-75.
Li, W., Zhu, T., and Guan, K-L. (2004) Transformation potential of Ras isoforms
correlates with activation of PI3K but not ERK. J. Biol. Chem. 279, 37398-406.
Kruger, R., Lee, J., Li, W., and Guan, K-L. (2004) Mapping netrin-receptor binding
reveals domains of Unc5 regulating its activity. J. Neuroscience 24, 10826-10834.
Wolf, M. T., Lee, J., Panther, F., Otto, E. A., Guan, K-L., and Hildebrandt, F. (2005).
Expression and Phenotype Analysis of the Nephrocystin-1 and Nephrocystin-4 Homologs
in Caenorhabditis elegans. J Am Soc Nephrol. 16, 676-687.
Corradetti, M., Inoki, K., and Guan, K-L. (2005) The stress-inducted proteins RTP801
and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J.
Biol. Chem. 280, 9769-72.
Yu, Y., Li, S., Xu, X., Li, Y., Guan, K-L., Arnold, E., Ding, J. (2005) Structural basis for
the unique biological function of small GTPase human Rheb. J. Biol. Chem. 280, 1709300.
Lee, J., Li, W.Q. and Guan, K-L. (2005) SRC-1 mediates UNC-5 signaling in C. elegans
Mol. Cell. Biol. 25, 6485-95.
Gan, B., Melkoumian, Z.K., Wu, X., Guan, K-L., and Guan, J-L. (2005) Identification of
FIP200 interaction with TSC1-TSC2 complex and its role in regulation of cell size
control. J. Cell Biol. 170, 379-89.
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KunLiang Guan, Ph.D.
115. Aurandt, J, Li., W., and Guan, K-L. (2006) Semaphorin-4D activates the MAPK
pathway downstream of plexin-B1. Biochem. J. 394, 459-64.
116. Li, W., Aurandt, J., Jürgensen, C., Rao, Y., and Guan, K-L. (2006) FAK and Src kinases
are required for netrin-induced tyrosine phosphorylation of UNC5. J. Cell Science 119,
47-55.
117. Chong-Kopera, H., Inoki, K., Li, Y., Zhu, T., Garcia-Gonzalo, F.R., Rosa, J.L., and
Guan, K-L. (2006) TSC1 stabilizes TSC2 by inhibiting the interaction between TSC2 and
the HERC1 ubiquitin ligase. J. Biol. Chem. 281, 8316-6.
118. Yang, Q., Inoki, K, Kim, E., and Guan, K-L. (2006) TSC1/TSC2 and Rheb have different
effects on TORC1 and TORC2 activity. Proc. Natl. Acad. Sci. USA 103(18):6811-6.
119. Inoki, K., Ouyang, H., Zhu, T., Lindvall, C., Wang, Y., Yang, Q., Bennett, C., Harada, Y.,
Stankunas, K., Wang, C., He, H., MacDougald, O., You, M., Williams, B., and Guan, KL. (2006) TSC2 integrates Wnt and cellular energy signals through a coordinated
phosphorylation by AMPK and GSK3 to regulate cell growth. Cell 126, 955-68.
120. Yang, Q., Inoki, K., Ikenoue, T., and Guan, K-L. (2006) Identification of Sin1 as an
essential TORC2 component required for complex formation and kinase activity. Genes &
Dev. 20, 2820-32.
121. Wan, M, Wu, X, Guan, K-L, Han, M, Zhuang, Y, Xu, T. (2006) Muscle atrophy in
transgenic mice expressing a human TSC1 transgene. FEBS Lett. 580, 5621-7.
122. Liu, G., Li, W., Li, X., Park, H-T., Jurgensen, C., Hanks, S.K., He, M-L., Wu, J., Guan,
K-L., and Rao, Y (2007) p130CAS Is Required for Netrin Signaling and Commissural
Axon Guidance. J. Neurosci. 27, 957-68.
123. Wang, C, Mao, X, Wang, L, Liu, M, Wetzel, M.D, Guan, K-L., Dong, L,Q, Liu, F.
(2007) Adiponectin sensitizes insulin signaling by reducing p70 S6 kinase-mediated serine
phosphorylation of IRS-1. J. Biol. Chem. 282, 7991-96.
124. Zhao, B., Wei, X., et al and Guan, K-L. (2007) Inactivation of YAP oncoprotein by the
Hippo pathway is involved in cell contact inhibition and tissue growth control. Genes &
Development 21, 2747-61.
125. Lee, CH, Inoki, K., Karbowniczek, M, Petroulakis, E, Sonenberg, N, Henske, EP, and
Guan, K-L. (2007) Constitutive mTOR activation in TSC mutants sensitizes cells to
energy starvation and genomic damage via p53. The EMBO J. 26, 4812-23.
126. Li, Y., Wang, Y., Kim, E., Beemiler, P., Wang, C-Y., Swanson, J., You, M., and Guan,
K-L. (2007) Bnip3 mediates the hypoxia-induced inhibition on mTOR by interacting with
Rheb. J. Biol. Chem. 282, 35803-13.
127. Lei, QY., Zhang, H., Zhao, B., Zha, Y., Bai, F., Pei, XH., Zhao, S., Xiong, Y., and Guan,
K-L., (2008) TAZ promotes cell proliferation and EMT and is inhibited by the Hippo
pathway. Mol. Cell Biol. 28, 2426-36.
128. Zhao, B., Ye, X., Yu, J., Li, L., Li, W., Li, S., Lin, JD., Wang, C-Y., Chinnaiyan, AM.,
Lai, Z-C., and Guan. K-L. (2008) TEAD mediates YAP dependent gene induction and
growth control. Genes & Development, 22, 1962-71.
129. Ikenoue, T., Inoki, K., Yang, Q., Zhou, X, and Guan, K-L. (2008) Essential Function of
TORC2 in PKC and Akt Turn Motif Phosphorylation, Maturation, and Signaling. EMBO
J. 27, 1919-1931.
130. Kim, E., Goraksha, P., Li, L., Neufeld, T.P., and Guan, K-L. (2008) Regulation of
TORC1 by Rag GTPases in nutrient response. Nature Cell Biol. 10, 935-945.
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KunLiang Guan, Ph.D.
131. Ikenoue, T., Inoki, K., Zhao, B., and Guan, K-L. (2008) PTEN Acetylation Modulates its
Interaction with PDZ Domain. Cancer Research, 68, 6908-12.
132. Buller, C., Loberg, RD., Fan, MH, Zhu, Q., Park, JL, Vesely, E., Inoki, K., Guan, K-L.,
and Brosius, FC. (2008) A GSK-3, TSC2, mTOR pathway regulates glucose uptake and
GLUT1 glucose transporter expression. Am J Physiol Cell Physiol, 295, C836-43.
133. Cao, C, Lu, S, Kivlin, R, Wallin, B, Card E, Bagdasarian, A, Tamakloe, T, Chu, WM,
Guan, K-L, Wan, Y. (2008) AMP-activated protein kinase contributes to UV- and H2O2induced apoptosis in human skin keratinocytes. J. Biol. Chem. 283, 28897-908.
134. Chen, C, Liu, Y, Liu, R, Ikenoue, T, Guan, K-L, Liu, Y, Zheng, P. (2008) TSC-mTOR
maintains quiescence and function of hematopoietic stem cells by repressing
mitochondrial biogenesis and reactive oxygen species. J Exp. Med. 205, 2397-2408.
135. Zhao, B., Kim, J., Ye, X., Lai, Z-C, and Guan, K-L. (2009) Both TEAD binding and WW
domains are required for the growth stimulation and oncogenic transformation activity of
YAP. Cancer Research 69, 1089-1098.
136. Liu, G., Li, W., Wang, L., Kar, A., Guan, K-L., Rao, Y, and Wu, J.Y. (2009) DSCAM
functions as a netrin receptor in commissural axon pathfinding. Proc. Nat. Acad. Sci. USA
106, 2951-2956.
137. Mori, H., Inoki, K., Münzberg, H., Opland, D., Faouzi, M., Villanueva, E.C., Ikenoue, T.,
Kwiatkowski, D., MacDougald, O.A., Myers, M.G. Jr., and Guan, K-L. (2009) Critical
role for hypothalamic mTOR activity in energy balance. Cell Metabolism 9, 362-74.
138. Zhao, S., Lin, Y., Xu., W., Jiang, W., Zha, Z., Wang, P., Yu, W., Li, Z., Gong, L., Peng,
Y., Ding, J., Lei, Q., Guan, K-L., Xiong, Y. (2009) Glioma-derived Mutations in IDH1
Dominantly Inhibit IDH1 Catalytic Activity and Induce HIF-1. Science 324, 261-5.
139. Chang, J., Wang, Z., Tang, E., Fan. Z., McCauley, L., Franceschi, R., Guan, K-L.,
Kresbach, PH., and Wang, C-Y. (2009) Inhibition of Osteoblast functions by IKK/NFkB in
osteoporosis. Nature Med. 15, 682-9.
140. Mori, H, Inoki, K, Masutani, K, Wakabayashi, Y, Komai, K, Nakagawa, R, Guan, K-L,
Yoshimura, A. (2009) The mTOR pathway is highly activated in diabetic nephropathy and
rapamycin has a strong therapeutic potential. Biochem. Biophys. Res. Comm. 384, 471-5.
141. Yu, W., Lin, Y., Yao, J., Huang, W., Lei, Q., Xiong Y., Zhao, S., and Guan, K-L. (2009)
Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in
Response to Nutrient Signals. J. Biol. Chem. 284, 13669-75.
142. Zhang, H., Liu-CY, Zha, ZY., Zhao, B., Yao, J., Zhao, SM., Xiong, Y., Lie, QY., and
Guan, K-L. (2009) TEAD transcription factors mediate the function of TAZ in cell
growth and epithelial-mesenchymal transition. J. Biol. Chem. 284, 13355-62.
143. Zhou, X., Ikenoue, T., Chen, X., Li, L., Inoki, K., and Guan, K-L. (2009) Rheb controls
misfolded protein metabolism by inhibiting aggresome and autophagy. Proc. Nat. Acad.
Sci. 106, 8923-8.
144. Mori, H., Inoki, K., Opland, D., Münzberg, H., Villanueva, EC., Faouzi, M., Ikenoue, T.,
Kwiatkowski, D., MacDougald, OA., Myers, MG., and Guan, K-L. (2009) Critical roles
for the TSC-mTOR pathway in -cell function. American J. Physiol. Endocrinol Metab
297, E1013-1022..
145. Zhao, B., Li, L., Wang, C-Y, and Guan, K-L. (2010) A coordinated phosphorylation by
Lats and CK1 regulates YAP stability through SCF-TRCP, Genes & Dev. 24, 72-85.
18
KunLiang Guan, Ph.D.
146. Li, Z., Zhao, B., Wang, P., Chen, F., Dong, Z., Yang, H., Guan, K-L., and Xu, Y. (2010)
Structural insights into the YAP and TEAD complex. Genes & Dev. 24, 235-240.
147. Zhao, S., Xu, W., Jiang, W., Yu, W., Lin, Y., Zhang, T., Yao, J., Zeng, Y., Li, H., Li, Y.,
Shi., J., An, W., Hancock, S.M., He., F., Qin, L., Chin, J., Yang, P., Chen, X., Lei, Q.,
Xiong, Y., Guan, K-L., (2010) Regulation of cellular metabolism by protein lysine
acetylation. Science 327, 1000-1004.
148. Wang, Q., Zhang, Y., Yang, C., Xiong, H., Lin, Y., Yao, J., Li, H., Xie, L., Zhao, W.,
Yao, Y., Ning, Z-B., Zeng, R., Xiong, Y., Guan, K-L., Zhao, S., Zhao, G-P. (2010)
Acetylation of metabolic enzymes coordinates carbon source utilization and metabolic
flux. Science 327, 1004-1007.
149. Alexander, A., Cai, SL., Kim, J., Nanez, A., Sahin, M., MacLean, KH., Inoki, K., Guan,
K-L., Shen, J., Person, MD., Kusewitt, D., Mills., GB., Kastan, MB., Walker, CL. (2010)
ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS. Proc.
Nat. Acad. Sci. 107, 4153-8.
150. Liang I., Kim, J., Okazawa, H., Zhao, J., Zhao, B., Yu, J., Chinnaiyan, A., Israel, M.,
Goldstein, L., Abujarour, R., Ding, S., and Guan, K-L. (2010). The role of YAP
transcription co-activator in regulating stem cells self-renewal and differentiation. Genes
& Dev. 24, 1106-1118.
151. Li, L., Kim, E., Yuan, H., Inoki, K., Goraksha-Hicks, Schiesher, R. L., Neufeld, T.P.,
Guan, K-L., (2010) Regulation of mTORC1 by the Rab and ARF GTPases. J. Biol.
Chem. 285, 19705-9.
152. Guan, K-L., Yu, W., Lin, Y., Xiong, Y., Zhao, S. (2010) Generation of acetyllysine
antibodies and affinity enrichment of acetylated peptides. Nature Protocol 5, 1583-1595.
153. Wang, C., Yoo, Y., Fan, H., Kim, E., Guan, K-L., and Guan, J-L. (2010) Regulation of
integrin 1 recycling to lipid rafts by Rab1a to promote cell migration. J. Biol. Chem.
285, 29389-405.
154. Zhang, D., Contu, R., Latronico, MV., Zhang, J.L, Rizzi, R, Catalucci, D., Miyamoto, S.,
Huang K, Ceci, M., Gu, Y., Dalton, N.D., Peterson, K.L, Guan, K-L., Brown, J.H, Chen,
J., Sonenberg, N., Condorelli, G. (2010) MTORC1 regulates cardiac function and
myocyte survival through 4E-BP1 inhibition in mice. J. Clin. Invest. 120, 2805-16.
155. Liu, C-Y, Zha, ZY., Zhang, H., Zhou, X., Huang, W., Zhao, D., Chan, S.W., Lim, C.J.,
Hong, W., Zhao, S., Xiong, Y., Lei, QY, and Guan, K-L. (2010) The Hippo tumor
pathway promotes TAZ degradation by phosphorylating a phosphodegron and recruiting
the SCFβ-TrCP E3 ligase. J. Biol. Chem. 285, 37159-69.
156. Kang, Y.J., Lu, M-K., and Guan, K-L. (2011) The TSC1 and TSC2 tumor suppressors
are required for proper ER stress response and protect cells from ER stress-induced
apoptosis. Cell Death & Differentiation. 18, 133-44.
157. Zhao, B., Li, L, Lu, O., Wang, L.H., Liu, C., Lei, Q., and Guan, K-L. (2011) Angiomotin
is a novel Hippo pathway component that inhibits YAP oncoprotein. Genes & Dev. 25,
51-63.
158. Xu, W., Yang, H., Liu, Y., et al, Guan, K-L., Zhao, S., and Xiong Y. (2011).
Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of -ketoglutaratedependent dioxygenases. Cancer Cell 19, 17-30.
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KunLiang Guan, Ph.D.
159.
160.
161.
162.
163.
164.
165.
166.
167.
168.
169.
170.
171.
Liu, C., Lv X., Li, T., Xu, Y., Zhou, X., Zhao, S., Xiong, Y., Lei, Q., and Guan, K-L.,
(2011). PP1 Cooperates with ASPP2 to dephosphorylate and activate TAZ. J. Biol.
Chem. 286, 5558-66.
Kim, J., Kundu, M., Viollet, B., and Guan K-L., (2011) AMPK and mTOR regulate
autophagy via direct phosphorylation of ULK1. Nature Cell Biol. 13, 132-141.
Hallows, WC, Yu, W., Smith, B.C,, Devires, M.K., Ellinger, J.J., Someya, S., Shortreed,
M.R., Prolla, T., Markley, J.L., Smith, L.M., Zhao, S., Guan, K-L., Denu, J.M. (2011)
Sirt3 Promotes the Urea Cycle and Fatty Acid Oxidation during Dietary Restriction. Mol.
Cell 41, 139-49.
Inoki, K., Mori, H., Wang, J., Suzuki, T., Hong, S., Yoshida, S., Blattner. S.M., Ikenoue,
T., Rüegg, R.A., Hall, M.N., Kwiatkowski, D.J., Rastaldi, M.P., Huber , T.B., Kretzler,
M., Holzman, L.B., Wiggins, R.C., Guan, K-L. (2011). mTORC1 activation in podocytes
is a critical step in the development of diabetic nephropathy. J. Clin. Invest. 121, 181196.
Zheng, M, Wang, Y.H, Wu, X.N, Wu, S.Q, Lu, B.J, Dong, M.Q, Zhang, H, Sun, P, Lin,
S.C, Guan, K-L, and Han. J. (2011). Inactivation of Rheb by PRAK-mediated
phosphorylation is essential for energy-depletion-induced suppression of mTORC1.
Nature Cell Biol. 13, 263-272.
Xie, X., Zhang, D., Zhao, B., Lu, M-K., You, M., Condorelli, G., Wang, C.Y., and Guan,
K-L. (2011) IKKε and TBK1 activate AKT by direct phosphorylation. Proc. Natl. Acad.
Sci. 108, 6474-9.
Chen, Y., Zhang, J., Lin, Y., Lei, Q., Guan, K-L., Zhao, S., Xiong, Y. (2011). Tumor
suppressor SIRT3 deacetylates and activates manganese superoxide dismutase (SOD2) to
scavenge ROS. EMBO Report. 12, 534-41.
Jiang, W., Wang, S., Xiao, M., Lin, Y., Lei, Q.Y., Xiong, Y., Guan, K-L., and Zhao, S.
(2011). Acetylation regulates gluconeogenesis by promoting PEPCK1 degradation via
recruiting the UBR5 ubiquitin ligase. Mol. Cell 43, 33-44.
Lv, L., Li, D., Zhao, D., Lin, R., Chu, Y., Zhang, H., Zha., Z., Xu, Y., Li, Z., Wang, G.,
Huang, Y., Zhao, S., Xiong, Y., Guan, K-L., Lei, Q-Y. (2011) Acetylation Targets the
M2 Isoform of Pyruvate Kinase for Degradation through Chaperone-Mediated Autophagy
and Promotes Tumor Growth. Mol. Cell, 42, 719-30.
Wu, Q., Liu, Y., Chen, C., Ikenoue, T., Qiao, Y., Li, C.S., Li, W., Guan, K-L., Liu, Y.,
and Zheng, P. (2011) The Tuberous Sclerosis Complex-Mammalian Target of Rapamycin
Pathway Maintains the Quiescence and Survival of Naive T Cells. J. Immunol. 187,
1106-12.
Gong, R., Li., L., Liu, Y., Wang, P., Yang, H., Wang, L., Cheng, J., Guan, K-L., and Xu,
Y. (2011) Crystal Structure of Gtr1p-Gtr2p complex reveals new insights into the amino
acid-induced TORC1 activation. Genes & Dev. 25, 1668-73.
Yoshida, S., Hong, S., Suzuki, T., Nada, S., Mannan, AM., Wang, J., Okada, M., Guan,
K-L., and Inoki, K. (2011) Redox regulates mTORC1 activity by modulating the
TSC1/TSC2-Rheb GTPase pathway. J. Biol. Chem. 286, 32651-60.
Zhou, X., Li, T., Feng, X., Hsiang, E., Xiong, Y., Guan, K-L., Lei, Q. (2012) Targeted
polyubiquitylation of RASSF1C by the Mule and SCFβ-TrCP ligases in response to DNA
damage. Biochem. J. 441, 227-36.
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KunLiang Guan, Ph.D.
172.
173.
174.
175.
176.
177.
178.
179.
180.
181.
182.
183.
184.
185.
186.
Zhao, B., Li, L., Wang, L., Wang, C.Y., Yu, J., and Guan, K-L. (2012) Cell detachment
activates the Hippo pathway via cytoskeleton reorganization to induce anoikis and inhibit
tumor metastasis. Genes & Dev. 26, 54-68.
Zhang, T., Wang, S., Lin, Y., Ye, D., Xiong, Y., Zhao, S., Guan, K-L. (2012)
Acetylation negatively regulates glycogen phosphorylase by recruiting protein
phosphatase 1. Cell Metabolism 15, 75-87.
Tang, F., Wu, Q., Ikenoue, T., Guan, K-L., Liu, Y., and Pan, Z. (2012) A critical role for
rictor in T lymphopoiesis. J. Immunol. 189, 1850-7.
Valbuena, N., Guan, K-L., and Moreno, S. (2012) The Vam6-Gtr1/Gtr2 pathway
activates TORC1 in response to amino acids in fission yeast. J. Cell Sci. 125, 1920-8.
Xiao, M., Yang, H., Xu, W., et al, Xiong, Y., and Guan, K-L. (2012)
KG-dependent histone and DNA demethylases by fumarate and succinate that are
accumulated in mutations of FH and SDH tumor suppressors. Genes & Development 26,
1326-38.
Huang, W., Lv, X., Liu, C., Zha, Z., Zhang, H., Jiang, Y., Xiong, Y., Lei, Q., and Guan,
K-L. (2012) The N-terminal phosphodegron targets TAZ for SCFβ-TrCP dependent
degradation in response to PI3K inhibition. J. Biol. Chem. 287, 26245-53.
Magee, J.A, Ikenoue T., Nakada, D., Lee, J.Y., Guan, K-L., and Morrison, S.J. (2012)
Temporal changes in PTEN and mTORC2 regulation of hematopoietic stem cell selfrenewal and leukemia suppression. Cell Stem Cell 11, 415-428.
Yu, F-X., Zhao, B., Panupinthu, N., Jewell, J. L., Wang, L., Zhao, J., Yan, H., Tumaneng,
K., Li, H., Fu, X-D., Mills, G. B., and Guan, K-L. (2012) Regulation of the Hippo-YAP
pathway by G-protein coupled receptor signaling. Cell 150, 780-91.
Purohit, A.A., Li, W., Qu, C., Guan, K-L., and Liu, G. (2012) DSCAM Associates with
UNC5C in Netrin-1-Mediated Growth Cone Collapse, J. Biol. Chem. 287, 27126-38.
Mo, J.S., Yu, F-X., Gong, R., Brown, J.H., and Guan, K-L. (2012) Regulation of the
Hippo-YAP pathway by protease activated receptor PAR. Genes & Dev. 26, 2138-2143.
Tumaneng, K., Schlegelmilch, K., Russell, R.C., Yimlamai, D., Basnet, H., Mahadevan,
N., Fitamant, J., Bardeesy, N., Camargo, F., and Guan, K-L. (2012) YAP mediates
crosstalk between the Hippo and PI3K-TOR pathways by suppressing PTEN via miR-29.
Nature Cell Biol. 14, 1322-1329.
Kim, J., Kim, Y.C., Fang, C., Russell, R.C., Kim, J.H., Fan, W., Liu, R., Zhong, Q., and
Guan, K-L. (2013) Differential regulation of distinct Vps34 complexes by AMPK in
nutrient stress and autophagy. Cell 152, 290-303.
Li, L. and Guan, K-L. (2013) Microtubule-associated protein/microtubule affinityregulating kinase 4 (MARK4) is a negative regulator of the mammalian target of
rapamycin complex 1 (mTORC1). J. Biol. Chem. 288, 703-708.
Yang, H., Liu, Y., Bai, F., Zhang, J.Y., Ma, S. H., Liu, J., Xu, Z., Zhu, H-G., Ling, Z.Q.,
Ye, D., Guan, K-L., and Xiong, Y. (2013) Tumor development is associated with
dramatic decreases of TET gene expression and global 5-hydroxymethylcytosine.
Oncogene, 32, 663-669.
Wang, P., Dong, Q., et al., Ye. D., Guan, K-L., Xiong, Y., Qin, L.X., Chiang, D.Y.
(2012). Mutations in isocitrate dehydrogenase 1 and 2 occur frequently in intrahepatic
cholangiocarcinomas and share hypermethylation targets with glioblastomas. Oncogene,
(in press).
21
KunLiang Guan, Ph.D.
187.
188.
189.
Zhao, D., Zou, S-W., Liu, Y., Zou, X., Mo, Y., Wang, P., Xu, Y-H., Dong, B., Xiong, Y.,
Lei, Q-Y., and Guan, K-L. (2013) Lysine-5 acetylation negatively regulates lactate
dehydrogenase A and is decreased in pancreatic cancer. Cancer Cell, 23, 464-476.
Russell, R.C., Tian, Y., Yuan, H., Park, H.W., Chang, Y.Y., Kim, J., Jim, H., Neufeld, T.,
Dillin, A., Guan, K-L. (2013) ULK1 induces autophagy by phosphorylating Beclin-1 and
activating Vps34 lipid kinase. Nature Cell Biol. (in press).
Yuan, H.X., Russell, R., and Guan, K-L. (2013) Regulation of PIK3C3/VPS34
complexes by mTOR in nutrient stress-induced autophagy. Autophagy (in press).
Peer-reviewed reviews
1.
Clemens, J.C., Guan, K-L., Bliska, J.B., Falkow, S., and Dixon, J.E. (1991) Microbial
Pathogenesis and tyrosine dephosphorylation: Surprising bedfellows. Mol. Microbiol. 5,
2617-2620.
2.
Guan, K-L. (1994) The mitogen activated protein kinase signal transduction pathway:
from the cell surface to the nucleus. Cellular Signaling 6, 581-589.
3.
Li, Y., Jenkins, C. W., Nichols, M. A.,Wu, X., Guan, K-L., and Xiong, Y. (1996). p16
family inhibitors of cyclin-dependent kinase. Pezcoller Foundation Journal. 7, 57-82.
4.
Guan, K-L. and Han, M. (1999) A G-protein signaling network mediated by an RGS
protein. Genes & Development 13, 1763-1767.
5.
Zhang, B. H. and Guan, K-L. (2001) Regulation of the Raf kinase by phosphorylation.
Experimental Lung Res. 27, 269-295.
6.
Zhan, X., Wishart, M. and Guan, K-L. (2001) Nonreceptor tyrosine phosphatase in
cellular signaling: Regulation of mitogen activated protein kinase. Chemical Rev. 101,
2477-2496.
7.
Chong, H., Vikis. H., and Guan, K-L. (2003) Regulation of the Raf family protein
kinase. Cellular Signaling. 15, 463-469.
8.
Wishart, M. and Guan, K-L. (2003) Protein phosphatase. Encyclopedia of Life Science
(Nature Publishing Group). In press.
9.
Guan, K-L. and Rao, Y. (2003) Signal Transduction Mechanisms Mediating Neuronal
Responses to Guidance Cues. Nature Rev. Neuroscience 4, 941-956.
10.
Li, Y., Corradetti, M., Inoki, K., and Guan, K-L. (2004) TSC2: filling the GAP of
mTOR signaling. TIBS 29, 32-38.
11.
Inoki, K., Qwyang, H., Li, Y., and Guan, K-L. (2005) TOR signaling in cell growth
control. Microbiol. and Mol. Biol. Rev. 69, 79-100.
12.
Inoki, K., Corradetti, and Guan, K-L. (2005) Disease associated with TOR
Dysregulation. Nature Genetics. 37, 19-24.
13.
Kruger, R., Aurandt., J., and Guan, K-L. (2005) Signaling of semaphorin in axon
guidance and other biological processes. Nature Rev. Mol. Cell. Biol. 6, 789-800.
14.
Inoki, K. and Guan, K-L. (2006) Complexity of the TOR signaling network. Trends in
Cell Biol. 16, 206-12.
15.
Corradetti, M. and Guan, K-L. (2006) Upstream of the mammalian target of rapamycin:
do all roads pass through mTOR? Oncogene 25, 6347-60.
16.
Lee, C.J, Inoki, K., and Guan, K-L. (2007) mTOR Pathway as a Target in Tissue
Hypertrophy. Annu. Rev. Pharmacol. Toxicol. 47, 443-67.
22
KunLiang Guan, Ph.D.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
Yang, Q. and Guan, K-L. (2007) Expanding mTOR signaling. Cell Research. 17, 666681.
Zhao, B., Lei, Q.Y., and Guan, K-L. (2008) The Hippo-YAP pathway: new connections
between regulation of organ size and cancer. Current Opinion in Cell Biology 20, 638646.
Kim, E. and Guan, K-L. (2009) RAG GTPases in Nutrient-mediated TOR Signaling
Pathway. Cell Cycle 8, 1014-1018.
Wang, W. and Guan, K-L. (2009) AMPK and Cancer. Acta Physiol. 196, 55-63.
Inoki, K. and Guan, K-L. (2009) Tuberous sclerosis complex, implication from a rare
genetic disease to common cancer treatment. Human Molecular Genetics 18, R94-100.
Zhao, B., Li, L., Lei, Q., and Guan, K-L. (2010) The Hippo-YAP pathway in organ size
control and tumorigenesis. Genes & Dev. 24. 862-874.
Zhao, B., Li L, and Guan, K-L. (2010) Hippo signaling at a glance. J. Cell Sci. 123,
4001-6.
Guan, K-L. and Xiong, Y. (2011) Regulation of intermediate metabolism by protein
acetylation. TIBS 36, 108-116.
Kim, J. and Guan, K-L. (2011) Amino acid signaling in mTOR activation. Ann. Rev.
Biochem. 80, 1001-32.
Zhao, B., Tumaneng, K., and Guan, K-L. (2011) The Hippo tumor suppressor pathway
in organ size control, tissue regeneration and stem cell self-renewal. Nature Cell Biol. 13,
877-83.
Russell, R.C. Fang, C., and Guan, K-L. (2011) An emerging role for TOR signaling in
mammalian tissue and stem cell physiology. Development, 138, 3343-56.
Inoki, K. Kim, J., and Guan, K-L. (2011) AMPK and mTOR in cellular energy
homeostasis and drug targets. Ann. Rev. Pharmacol. Toxicol. 52, 381-400.
Oermann, E.K., Guan, K-L., and Xiong, Y. (2012) Alterations of metabolic genes and
metabolites in cancer. Semin. Cell Dev. Biol. 23, 370-80.
Tumaneng, K., Russell, R. C., and Guan, K-L. (2012) Organ size control the Hippo and
TOR pathways. Current Biology 22, R368-R379.
Hong, W. and Guan, K-L., (2012) The YAP and TAZ transcription coactivators: key
downstream effectors of the mammalian Hippo pathway. Seminar in Cell Dev. Biol. 23,
785-93.
Xiong, Y. and Guan, K-L. (2012) Mechanistic insights into the regulation of metabolic
enzymes by acetylation. J. Cell Biol. 198, 155-64.
Yang, H., Ye, D., Guan, K-L., and Xiong, Y. (2012). IDH1 and IDH2 mutations in
tumorigenesis: mechanistic insights and clinical perspectives. Clin. Cancer Res. 18,
5562-5571.
Yan, H-X., Xiong, Y., and Guan, K-L. (2013) Nutrient sensing, metabolism, and cell
growth control. Mol. Cell. 49, 379-387.
Jewell, J.L. and Guan, K-L. (2013) Nutrient signaling to mTOR and cell growth. TIBS.
(in press).
Yu, F. and Guan, K-L. (2013) The Hippo Pathway: Regulators and Regulations. Genes
& Dev. 27, 355-371.
Jewell, J.L. Russell, R.C., and Guan, K-L. (2013) Amino acid signaling, mTOR. Nature
Rev. Mol. Cell. Biol. 14, 133-139.
23
KunLiang Guan, Ph.D.
Non peer-reviewed publications
1.
Weiner, H., Wang, T., Guan, K-L., Farres, J., Pak, Y.K., Saigal, D., Cunningham, S.,
and Jeng, J.J. (1988) Application of Molecular Biological Techniques in Alcohol
Research: Study of Aldehyde Dehydrogenase in Biomedical and Social Aspects of
Alcohol and Alcoholism (Kuriyama, K., Takada, A., & Ishii, H., eds) pp. 377-380,
Elsevier Science Publishers B.V.
2.
Guan, K-L., and Weiner, H. (1987) Molecular biology studies of beef liver aldehyde
dehydrogenase in Enzymology and Molecular Biology of Carbonyl Metabolism:
aldehyde dehydrogenase, aldo-keto reductase and alcohol dehydrogenase (Weiner, H. &
Flynn, T.G., eds) pp. 15-24, Alan R, Liss, Inc., New York.
3.
Woodford, T.A., Guan, K-L., and Dixon, J.E. (1991) Expression of rat PTP1 in Normal
and transformed cells. Adv. Prot. Phosphatases 6, 503-524.
4.
Dixon, J.E., Hakes, D., and Guan K-L. (1993) Improved vectors for expression and
purification of recombinant proteins. Methods in Genetics 2, 44-53.
5.
Guan, K-L. and Dixon, J.E. (1993) Bacterial and viral protein tyrosine phosphatases.
Seminars in Cell Biology . 4, 389-396.
6.
Wang, Y. and Guan, K-L. (1996) Regulation and function of the mitogen activated
protein kinase (MAPK) signal transduction pathway. Bio/Pharma Quaterly. 1(4), 23-26.
7.
Vikis, H. and Guan, K-L. (2002) Regulation of the Ras-MAPK pathway at the level of
Ras and Raf. Genetic Engineering (Jane Setlow eds) 24, 49-66.
8.
Lanigan, T., Vikis, H., Guan, K-L. (2003) Control of the Ras-MAPK signaling pathway
by phosphorylation and scaffolding proteins. Res. Signpost 37, 661-615.
9.
Vikis, H. and Guan, K-L. (2004) Glutathione-S-transferase-fusion based assays for
studying protein-protein interactions. Methods Mol. Biol. 261, 175-186.
10.
Li, Y, Inoki, K, Vikis, H, Guan, K-L. (2005) Measurements of TSC2 GAP Activity
Toward Rheb. Methods Enzymol. 407, 46-54.
11.
Zhao, S. and Guan, K-L. (2008) Substrate selectivity APPLies to Akt substrate
selectivity. Cell 133, 399-400.
12.
Zhao, B., Lei, Q., and Guan, K-L. (2009) Harness the Power: New Insights into the
Inhibition of YAP/ Yorkie. Dev. Cell. 16, 321-322.
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