CHAPTER 1 : Introduction – The Scientific Study of Life I. The Organization of Life – defining the scope of biology (AIM: How is life organized) (Figure 1.1) A. Levels at which life is organized 1. Biosphere 2. Ecosystem 3. Community 4. Population 5. Organism 6. Organ System 7. Organ 8. Tissues 9. Cell 10. Molecule 11. Atoms Figure 1.1 – Life’s hierarchy of organization Hierarchy – each level builds on the one below it. Emergent Properties – new properties emerge as one goes from a lower level to a higher level Structure-Function Relationship – these new properties are based on the new structures that are generated. Structure is directly related to function (A hammer has a structure for hitting nails and a screwdriver has a structure for turning screws.) NOTE: Most Biologists tend to focus on one particular level (Ex. E. O. Wilson – studies social behavior of animals, I studied proteins and DNA (molecular level)) II. Scientists use two main approaches to learn about nature (AIM: What is Science?) A. What is Science? 1. Write down your definition of science… 2. How many of you have done or used science to know something? a) Ex. Who taught you that objects have the tendency to fall towards the Earth? b) Can you prove to me that the next object I drop will fall? c) Can you prove that the sun will set today and night will come? d) Can you prove anything to me? e) Can you prove that I am standing here?? (1) Everything we know is based on data collected by instruments. In this case the instruments are our eyes, ears, etc… (2) Could there be another explanation??? (3) There can always be another explanation… (4) Conclusion: we cannot prove with 100% certainty anything and therefore we know nothing with 100% certainty. f) Everything we know is based on a level of uncertainty… (1) The edge of the universe…high uncertainty (2) My standing in front of the room…low uncertainty (but not zero uncertainty) (3) When someone is determined to be guilty of a crime they are guilty beyond a reasonable doubt, but we can never say the we have proven guilt. (4) Conclusion: we aim to make what we know have as little uncertainty as possible and we use science to do this… 3. derived from latin word meaning “to know” 4. A way (method) of knowing 5. The process employed to try and understand the history of the natural universe, and how the universe works using observable evidence. 6. So how does one do science? B. The two main approaches to learning about nature: 1. Discovery Science (descriptive science) a) Based on verifiable observations and measurements (1) Example: an ecologist observes that newborn flying fox cling to the mothers chest during flight (Figure 1.2) – Show Wild Parrots of Telegraph Hill (Mark Bittner’s cherry head conures) 41:58 2. Hypothesis-Driven Science (hypothetico-deductive reasoning) a) Discovery science leads to questions being asked, which will lead to suggested explanations of what you are observing – HYPOTHESIS **The textbook may mislead you into thinking that discovery science and hypothesis driven science are separate entities, but they work together – observation (discovery science) leads to hypotheses (hypothesis driven science) b) Flashlight demonstration (1) OBSERVATION – My flashlight doesn’t work (2) QUESTION – What’s wrong with my flashlight? (3) HYPOTHESIS – The flashlight’s batteries are dead (4) PREDICTION – If I replace the batteries the flashlight should work (5) EXPERIMENT – Replace the batteries with new ones, (experiments are really just ingenious ways of generating new observations) (6) ANALYZE RESULTS – did the flashlight go on or not?? (a) If not, we need to modify our hypothesis and try again. (b) If it does, then our hypothesis survives c) This process is called the SCIENTIFIC METHOD (1) Observations – from discovery science, earlier tests (experiments), etc… (2) Questions – about unclear aspects of the observations (How? Why? When? Where? Etc…) (3) Hypothesis – a tentative explanation for an observation (4) Predictions – What you would predict to happen if your hypothesis were correct (5) Tests (Experimental) – Determine if the predictions are supported or not. The results are used as new observations for another investigative cycle d) There are two different types of reasoning present in the Scientific Method: Inductive vs. Deductive reasoning (1) Inductive (a) Derives general principles from a large number of specific observations – everything I drop falls to the earth, therefore all things fall to the earth (b) Observations hypothesis (c) specific to general (2) Deductive (a) Sherlock Holmes (b) From general hypotheses we try and make specific predictions. (c) Hypothesis conclusions e) Other examples of the scientific method in action (1) Gravity (2) Your doing science right now!! (3) Medical doctors (4) My drive to work example (5) Darwin – Theory of Evolution (a) Publish results for scientific community (b) Theory vs. Law (i) Theory is a collection of hypotheses all about the same general principle that has stood the test of time (never been broken by any observation) (ii) Automobile analogy (a) Theory of Evolution, Cell Theory, etc… (iii)Law – concise explanation of an action or set of actions (sometimes written as a simple equation) (a) Law of Gravity, Laws of Motion, Laws of Thermodynamics, etc… (c) Scientists try to falsify (disprove) hypotheses, it doesn’t prove anything – ex. It can’t prove that the next thing I let go of will fall to the Earth. (d) Science is tentative (i) Another example: Newton and Einstein – nova video f) Science is fallible (liable to be erroneous) (1) Doubt – the greatest tool of any scientist (and everyone else) (2) Don’t always believe what you hear and read, and question everything regardless of who the information is coming from. g) Real-life Example – plants in brackish tidal marsh h) Lancet – mental health article C. Controlled Experiments – A special class of experiments 1. Example – Earth Worm Droppings as fertilizer a) Control group b) Experimental group c) Variable – that which is changed or altered in the experiment (1) Independent variable – variable adjusted by the scientist – there should be only ONE! (2) Dependent variable – the variable that is being measured by the scientist d) Experimental validity (1) Randomly chosen sample group (2) Large sample size (3) Must be done more than once (many trials) 2. Experiments MUST be repeatable by others D. Design your own experiments E. A case study from the Biological Science (A PredatorPrey System - mimicry) 1. OBSERVATIONS – 1) Jumping spiders defend theirs territories against other members of their population by waving their legs at them. 2) Certain fly species like the snowberry fly, when approached by jumping spiders, wave their wings, which have markings that resemble spider legs 2. QUESTION – What is the function of the flies’ wing markings and waving behavior? 3. HYPOTHESIS – The markings and wing waving increase survival of the flies by causing jumping spiders to flee 4. PREDICTION – If one masks the flies wing marking with dye, the spider should pounce on these flies more often 5. EXPERIMENT 6. PREDICTED RESULT The diversity of life can be arranged into three domains (or 5 kingdoms!?)- [15-14-ClassificationAnim.mov] III. A. ~1.5 million known species / estimated 30 million species on planet – we need to organize!!!! B. Species – populations of organisms who have the potential to interbreed and produce FERTILE offspring C. Taxonomy – branch of science concerned with naming and classifying the diverse forms of life D. Prokaryotes vs. Eukaryotes 1. Prokaryotes – lack a nucleus 2. Eukaryotes – have a nucleus and internal structures called organelles. E. The 3 domain system: 1. Bacteria (Eubacteria) - prokaryotes 2. Archaea (Archaeabacteria) - prokaryotes 3. Eukarya - eukaryotes F. The 5 kingdom system 1. Monera – single celled, only cells without a nucleus 2. Protista – Protozoans and algae (predominantly singlecelled) 3. Fungi – molds, yeast, and mushrooms – decompose and absorb nutrients from dead organic matter 4. Plantae – complex form, photosynthesis (autotrophs – autos = self, trophe = nutrition (Greek)) 5. Animalia – complex form, eat other organisms (heterotrophs – heterone = (an)other, trophe = nutrition) G. Classification System (Taxonomy) – human taxonomy 1. Kingdom 2. Phylum 3. Class 4. Order 5. Family 6. Genus 7. Species 8. Kings Play Chess On Flat Glass Squares, King Phillip Came Over From Germany Singing H. BINOMIAL NOMENCLATURE - Carolus Linnaeus 1. Scientific names are applied by using Genus species I. Mention E.O. Wilson and the encyclopedia of life IV. Phylogenetic trees strive to represent evolutionary history A. Phylogeny (phylon = tribe, genesis = origin (Greek)) – the evolutionary history of a group of organisms B. Phylogenetic trees – diagrams that trace evolutionary relationships as best they can be determined. 1. It is simply a very large family tree looking back to our ancestors. If we trace the ancestry of all organisms back, we will meet at one organism, at one place in time. (For a great book on this subject try The Ancestors Tale by Richard Dawkins). V. Dichotomous (Identification) Key A. How would you go about figuring out the species assignment of a living thing? B. Dichotomous keys are a method for doing just that C. It is very similar to the game – 20 questions D. Each question narrows down the possibilities further and further until there is only one answer. E. Tree Branch demonstration using either website below: 1. http://www.dnr.state.wi.us/org/caer/ce/eek/veg/treekey/i ndex.htm 2. http://www.uwsp.edu/cnr/leaf/Treekey/tkframe.htm VI. Unity in Diversity: All forms of life have common features – Characteristics of Life: A. Although life appears to be quite diverse, we all share common properties. Taken together, these properties distinguish living (biotic) from non-living (abiotic). 1. Order (organized): all organisms are made of one or more cells (the basic units of structure and function). The internal workings of cells as well as the interactions between cells in multi-cellular organisms are highly ordered (hierarchy of life). a) All cells share similar molecules (ex. DNA) b) The way that genetic information is encoded in DNA is the same for all cells in all known organisms c) The information stored in DNA is used to build the organism and give the organisms the properties that distinguish living (biotic) from non-living (abiotic) d) DNA is a parts list – It is a list of every protein in the organism and how to assemble them. 2. Regulation (homeostasis): The environment outside an organism may change markedly, but regulatory mechanisms maintain the internal environment of the organism within life sustaining limits. a) The maintenance of our internal environment is called homeostasis. (1) Ex. When you exercise, you will generate heat and begin to sweat. Sweating is the bodies way of cooling itself down in an attempt to maintain the body’s temperature around 98.6F – homeostasis. (2) What about if you get too cold? 3. Growth and Development 4. Energy Utilization – organisms must take in energy and transform it to perform life’s activities. In order to do this cells carry out various chemical reactions. a) Metabolism – combination of all the chemical reactions that occur in an organism b) Ex. Moving your arm requires energy. This energy, in the case of heterotrophs, comes from the food we eat. 5. Response to the environment a) Ex. You are hiking in the woods when you start to smell smoke and see large flames in the distance. You turn around and head in the other direction. b) Ex. You touch a hot iron and pull your hand away. 6. Reproduction: the passing of hereditary information to new organisms of the same type 7. Evolution: the capacity of a species to change over time. It is the central unifying feature of life from its inception almost 4 billion years ago. VII. The Life Processes: these are the processes required by living things to maintain life. 1. Nutrition – all the activities by which an organism obtains food from the environment and breaks it down into a form that can be absorbed by its cells. 2. Transport – involves the absorption and distribution (circulation) of essential materials throughout the organism. 3. Cell Respiration – Cell respiration (different from breathing) releases energy from food by a complex series of chemical reactions. 4. Excretion – process by which wastes of metabolism are removed from the organism. 5. Synthesis – the process by which simple molecules are combined chemically to form more complex molecules. 6. Growth – increase in size of an organism resulting from the synthesis of food substances into new materials and new structures. 7. Responding to internal and external stimuli 8. Reproduction – process by which organisms give rise to offspring. B. Non-living (abiotic) things may carry out some of these processes, but living (biotic) things carry out ALL of them. C. HNTRSGERMR VIII. Evolution explains the unity and diversity of life – it is the core theme of all life A. THEORY – a widely accepted explanatory idea that is broad in scope and supported by a large body of evidence B. Charles Darwin – On the Origin of Species by Means of Natural Selection – showed that evolution can be explained by the diversity of life and its underlying commonalities C. Theory of Natural Selection – proposed by Darwin to explain evolution 1. Heritable variations must occur in a population 2. Environmental factors favor one variation over another in terms of survival and reproductive success 3. The evolution of new species comes from the accumulation of small changes over long periods of time D. ADAPTATIONS – features that evolved by means of natural selection E. Evolution does NOT create!!! It selects from what is available!! IX. Living organisms and their environment form interconnecting webs A. Living things are intertwined with their environment, which included both living and non-living entities. B. Ecosystems rely on the flow of chemical nutrients and energy 1. Chemical - The most basic chemicals needed for life (atoms making CO2, H2O, O2, N2, Minerals, etc...) are constantly being recycled between living and non-living entities. 2. ENERGY a) 1st law of thermodynamics - energy cannot be created or destroyed, it can only change form. b) Energy, Work, and Force c) Three sources of energy on Earth (1) Sun (2) Geothermal (3) Gravitational pull of the moon d) Flow of Energy example (1) Energy comes from the sun in the form of light (2) Plants (some prokaryotes and protists also) trap the light’s energy and use it to make food molecule from CO2 and H2O – light energy now stuck in the food molecule (3) Animals need to eat the plants, etc… to get the energy (4) Other animals will eat these animals to get the energy, and so on. X. We are biological organisms and thus we are intimately connected to biology in our lives: A. Birth and death, population, nutrition, exercise, dieting, medicine, agriculture, endangered species, pollution, environmental changes (global warming) etc…