http://www.coleparmer.com/catalog/product_view.asp?sku=9903722
Improved design prevents the trapping of liquid
Use these Soxhlet extraction systems to extract soluble components from a solid
sample into an organic solvent. Place a sample in the thimble and heat the solvent
contained in the flask. As the solvent vapors rise (passing through the extraction
thimble), they enter the water-cooled condensor, and liquify. When the liquid level in
the extractor reaches the top of the siphon tube, siphoning action returns the extractenriched solvent to the flask. Soxhlet-type extraction is used for continuous extraction
of solids with a suitable solvent. The improved design includes a protected siphon
tube, an enlarged vapor tube, and sloping seals to prevent the trapping of liquid. TS
joints connect the condenser to the extractor and the extractor to the flask. Uses paper,
alundum, or extraction thimbles (order separately). Purchase Soxhlet glassware as a
complete assembly or as individual components. What's included in a complete
assembly: condenser, extractor body, and flask.
SpecificationsCondenser type Friedrich condenser
Flask Type Flat bottom
Flask capacity 250 ml
Body ID and length 41 x 157 mm
Condenser Joint Size 45/50
Accessories
EW-29821-00 Extraction Thimbles, 22 mm H x 80 mm OD; 25/pack
Availability: In Stock
Price: $43.50/PK
Qty:
EW-29821-04 Extraction Thimbles, 33 mm H x 80 mm OD; 25/pack
Availability: In Stock
Price: $53.00/PK
Qty:
EW-99036-50 Extraction thimble for soxhlet extraction glassware systems, 25 mL
Availability: Lead time for this item is 21 days.
Price: $29.50/EA
Qty:
Literature
05-06 Catalog
http://www.laboratory-glasswares.com/fractionating-assembly.html
77-515 SOXHLET EXTRACTION APPARATUS
Consists of a Flask, Extractor and
Condenser.
Extractor
Code Capacity
(ml.)
Extractor Extractor
Socket
Cone
Flask
Capacity
(ml.)
01
20
B24
B19
50
02
40
B29
B24
100
03
60
B34
B24
150
04
100
B34
B24
250
05
100
B40
B24
250
06
200
B50
B24
500
07
400
B50
B24
1000
08
600
B50
B24
2000
09
1000
B55
B34
3000
10
2000
B55
B34
5000
11
3000
B55
B34
10000
http://pjms.com.pk/issues/janmar05/article/article11.html
Reflux extraction: Aqueous and methanol extractions were performed by
the following method. 50 g of blackseed powder were used with 300 ml of
water or methanol with an extraction period of 10-12 hour. The extracts were
filtered using filter paper and the solvents were evaporated using rotary
distillation apparatus. In order to obtain a completely dry extract, the
resultant extracts were transferred to glass dishes and were left in a 50oC
oven for 24 hour. Then, they were left at 4oC until assessment of their
antimicrobiological activities.
http://www.chem.ualberta.ca/~orglabs/x61web/frames/psl4/fr_psl4_insight.html
PSL 4: Background Information
about
Trimyristin from Nutmeg
Solid-liquid extraction
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In this experiment, extraction involves the removal of a component or
group of components from plant tissue.
This type of extraction, solid-liquid extraction, is accomplished by
allowing hot solvent to leach out the compounds from the solid tissue.
Since different solvents have different solubility properties, choosing a
solvent is an important factor when extracting compounds from the
plant material (e.g., nutmeg). The solvent should dissolve the desired
compound and preferably nothing else, although this is generally
difficult to achieve.
In this experiment, trimyristin, for example, can be selectively removed
from nutmeg by extraction with dichloromethane.
The mixture is usually heated under reflux. The reflux technique allows
extraction of the solid at elevated temperature without loss of solvent
by evaporating.
Reflux

A reflux apparatus [cooled condenser inserted into a round-bottom
flask] is used to prevent loss of liquid during continuous boiling. The
uprising vapors of the solvent are cooled by the condenser and the
droplets fall back into the solid-liquid mixture in the round-bottom flask.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S167891992005000400007
3. Determination of the flavonoid and polyphenol content
3.1. Flavonoids content
The content of flavonoids was determined as described by other authors(9, 17),
based on the UV absorption of Al-Cl3-flavonoid complexes, and was expressed as
the total content of quercetin. Briefly, 2 g of the extract were extracted under
reflux for 30 min with 50 ml of 50% methanol and 5 ml of this extract was then
completed to 50 ml with 80% methanol. Aliquots (2 ml) of this solution (n=4)
were mixed with 2 ml of methanol containing 5% anhydrous aluminum chloride
(AlCl3; complexing agent) and completed to 10 ml with 80% methanol. After 30
min, the absorbances were read at 420 nm against a blank containing 80%
methanol (8 ml) and 5% AlCl 3 (2 ml). The percentage of flavonoids (%FL) was
calculated from a standard curve of quercetin (Sigma-Aldrich®) prepared in
methanol (0, 4, 8, 12, and 16 µ g/ml).
3.2. Polyphenol content
The content of polyphenols was determined as described by other authors(13,
22), using an adaptation by Reicher et al. (22). Briefly, 2 g of the extract were
extracted under reflux with 50 ml of 50% ethanol, and 5-ml aliquots of this
solution (n=3) were completed to 50 ml with 50% ethanol. From this solution, 5ml aliquots were withdrawn and completed to 25 ml using distilled water. Five
hundred microliters of phosphomolybdotungstic reagent was added to 1 ml of this
solution and the volume was completed to 10 ml with 15% sodium carbonate
solution. After 30 min, the absorbance at 720 nm was read against a blank of
15% sodium carbonate solution. The percentage of polyphenols (%PP) was
determined from a standard curve (5, 10, 15, 20, 25, 30, 35, and 40 µ g/ml) of
pyrogallol (Sigma-Aldrich®).
heated not to boil the organic solvent but to create enough vapor pressure to produce a steady flow of
liquid drops from the condenser at the top of the soxhlet apparatus. Once the solvent in the upper
chamber rises above the relief arm, the solvent is returned to the round bottom flask and the process
repeats itself. Most extractions are run for at least four hours and some extractions are run from twelve
to twenty-four hours. Timing experiments must be run to determine the best analysis time for the best
extraction results.
http://www.instrumentalchemistry.com/sampleprep/pages/soxhlet.htm