Conservation of Florida’s Coral Reefs through Controlled Propagation – Year 2 “Protect Our Reef” Grant -Interim Report (April 1 – September 30, 2007) Principal Investigators Kevan Main Kim Ritchie Submitted to Dena Smith Mote Marine Laboratory October 19, 2007 Mote Technical Report # 1215 PROJECT GOALS The goals of this project are to develop optimal culture techniques to produce four selected scleractinian corals for reef restoration research, to evaluate the health of natural and cultured stocks, and to disseminate project results to the user groups in the Florida Keys and the scientific research community. The cultured coral fragments produced in this project will be used to scientifically evaluate coral re-establishment to increase success rates of restoration efforts. PROJECT ACCOMPLISHMENTS Objective 1: Develop and refine culture methods to produce four scleractinian coral species impacted by boat groundings and other catastrophic events for restocking research in the Florida Keys. The Coral Aquaculture Laboratory at TRL has colonies from 19 species of scleractinian corals in the aquaculture tank systems in our environmentally controlled laboratory, which are being monitored for growth and survival. The focus of this project is to develop the culture technology for the four coral species shown in figures 1-4. Figure 1. Acropora cervicornis Figure 3. Montastrea annularis Figure 2. Acropora palmata Figure 4. Montastrea cavernosa During this reporting period, we continued to culture these species and monitor the environmental parameters and feeding rates in the culture systems. New colonies were not collected during this reporting period; however, colonies of some species were fragmented to increase the number of corals in the culture systems. Objective 2: Culture, fragment and document growth rates of four coral species in land-based fully recirculating, environmentally friendly aquaculture systems. Growth measurement data was collected in May and September 2007 for all coral colonies in the indoor culture systems. Coral colonies were photographed from two views (top view to determine laminar growth; side view to determine growth in height). In May, we initiated a growth study with M. annularis at six different light levels in all indoor tanks. A total of 36 (approximately 1 inch) coral colonies were fragmented, mounted and placed on the coral culture bars in 3 100-gallon and 3 200-gallon tanks (see Figure 5). All colonies were photographed in May to obtain initial growth measurements and again in September to measure growth. The data from those photographs has not yet been analyzed. In October, this growth study will be expanded to include A. cervicornis and M. cavernosa. Figure 5. Partial view of Tank 100A with M. annularis on culture bars. Objective 3: Establish captive broodstock colonies for the four selected coral species to ensure availability for restocking and research. Broodstock colonies for all four selected coral species are being maintained in the broodstock reserve facility at Mote’s Tropical Research Laboratory at Summerland Key. Objective 4: Monitor coral health at the time of collection for cultivation and during fragment cultivation. This objective is linked to field collections of new coral colonies. Since no new colonies were collected in the field, no progress was made on this objective during this reporting period. Objective 5: Disseminate project results through public meetings, presentations and outreach to stakeholders, researchers and the general public. No progress has been made on this objective during this reporting period. PROJECT STATUS A change in project staffing occurred in July and our biologist, David Lackland, is no longer working on this project. We have not identified an individual to replace Mr. Lackland; therefore, in the interim, Drs. Kevan Main and David Vaughan have been managing the project activities and the experimental studies. Other staff at Mote’s Tropical Research Lab (Erich Bartels, Cory Walters and Steve Davis) are also assisting in maintaining the corals during this interim period.