2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC 2009 List of Proposals from the Department of Agriculture and Agri-Food Canada (AAFC) (The List includes 49 proposals in total.) Project ID Contact Project Title Internship (Use ‘CTRL+click’ on the ‘Project ID’ to follow the hyperlink to (year_location_#) (AAFC Scientist) Duration the proposal description) (months) David Ehret Improving Nutritional Quality of Organic and Hydroponic 12 2009_Agassiz_01 Tomatoes Todd Kabaluk Yield increase of corn following treatment of seed with 12-24 2009_Agassiz_02 Metarhizium anisopliae: the identification of causal factors David De Koeyer Genomics-assisted potato breeding to meet global 12 2009_Fredericton_01 challenges Rong Cao Antioxidant properties and potential health benefits of 12 2009_Guelph_01 pigmented vegetables John Shi ‘Green’ extraction process to produce functional food 12 2009_Guelph_02 ingredients from agricultural material Alireza Navabi Genotype by environment interaction and mega12 2009_Guelph_03 environment investigation in long term common bean (Phaseolus vulgaris L.) regional variety testing in Ontario Kangfu Yu Coarse mapping of an F2 bean population BBSS x Jalo 24 2009_Harrow_01 EEP558 by Diversity Arrays Technology (DArT) Les Shipp Evaluation of bumble bees as vectors for microbial control 24 2009_Harrow_02 agents (viruses and fungi) for arthropod pest management Vaino Poysa EcoTILLING Molecular analysis of glycinin genes in 24 2009_Harrow_03 soybean (Glycine max) mutant lines. Martin Kalmokoff Manipulation of intestinal bacterial communities; an 12 2009_Kentville_01 investigation into the impact of dietary fibre and prebiotics. Benoît Bizimungu Identification and selection of potato clones with novel 24 2009_Lethbridge_01 starch properties for food and industrial uses 2009_Lethbridge_02 John Lu Genetic engineering of cereal crops to produce novel starch 12-24 Ranjana Sharma Assessment of molecular and genetic diversity in specific 12 2009_Lethbridge_03 bacterial population from beef cattle - Implications for the Food safety Wenzhu Yang Develop alternative growth promoter in ruminant 12 2009_Lethbridge_04 production Xiying Hao Hydrogen energy production through bio-fermentation of 12 2009_Lethbridge_05 livestock manure 2009_London_01 Deena Errampalli Management of postharvest diseases in tree fruits 19 2009_London_02 Aiming Wang Developing Novel Genetic Resistance to Plum Pox Virus 24 Frédéric Marsolais Functional genomic analysis of seed storage protein 24 2009_London_03 deficiency in common bean (Phaseolus vulgaris): regulation of sulphur amino acid content. George Lazarovits Development of sustainable agricultural systems through an 12-24 2009_London_04 understanding of interactions between microorganisms in the rhizosphere and corn roots Mark Gijzen Plant pathogenic Phytophthora: molecular determinants of 12 2009_London_05 virulence Pervaiz A. Abbasi Developing reduced-risk and biological-based strategies for 24 2009_London_06 management of soil-borne diseases of vegetable crops and promotion of plant growth 2009_London_07 Rima Menassa Characterizing plant-produced human interleukin-24 24 2009_Ottawa_01 Allen Xue Biological management of fusarium head blight in wheat 12-24 2009_Ottawa_02 Lana M. Reid Phytochemical Resistance Mechanisms to Corn Pests 12 Carlos Monreal Management and total use of agricultural residues for 12-24 2009_Ottawa_03 biofuels, bioproducts and bioenergy (Update: January 28, 2009) Page 1 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Elizabeth Pattey 2009_Ottawa_04 2009_Quebec_01 Yves Castonguay Eric van Bochove 2009_Quebec_02 Gaëtan Tremblay 2009_Quebec_03 2009_Quebec_04 2009_Saskatoon_01 2009_Saskatoon_02 2009_Saskatoon_03 2009_Saskatoon_04 2009_Saskatoon_05 2009_Sherbrooke_01 2009_Sherbrooke_02 2009_Sherbrooke_03 2009_SJSR_01 Marie-Josée Simard Kevin Rozwadowski Kevin Rozwadowski Kevin Rozwadowski Russell K. Hynes Yong-Bi Fu Lucie Masse Luigi Faucitano Daniel Ouellet Shahrokh Khanizadeh Gaétan Bourgeois 2009_SJSR_02 2009_StHyacinthe_01 2009_StJohns_01 2009_Summerland_01 Byong H. Lee Samir C. Debnath Dan O’Gorman Helene Sanfacon 2009_Summerland_02 2009_Summerland_03 2009_Summerland_04 Joan Cossentine Howard Thistlewood A. K. Singh 2009_Swift Current_01 2009_Winnipeg_01(Morden) 2009_Winnipeg_02 Anfu Hou Mark Jordan (Update: January 28, 2009) Adapting micrometeorological techniques for verifying GHG emissions from agricultural fields and paddocks to desired conditions Identification of functional markers associated to superior freezing tolerance in alfalfa. Develop a particulate phosphorus (PP) component for a risk indicator of water contamination by P from Canadian agricultural land Fractions protéiques et glucidiques de diverses espèces fourragères / Protein and carbohydrate fractions in different forage species Evaluation of invasive weed distribution and spread into crop fields Enhancing quality and function of recombinant proteins produced in seeds DNA recombination and repair in plants Seed-based production of recombinant proteins Formulation development for baculovirus Molecular characterization of wheat adaptation genes Ammonia recovery by volatilization and capture Effect of vehicle design and transport distance on stress response and carcass and meat quality of three pig lines Factors influencing variations in milk protein constituents Use phytochemical as marker to select strawberry and raspberry lines for processing (juice & fruit wine) Impact of climate change and variability on the productivity of field grown vegetable crops: A bioclimatic modelling approach Overproduction of extremophilic bacterial enzymes (lactase and lipase) for the industrial applications Bioreactor micropropagation and genetic fidelity in berry crops Diagnostic Technologies and Management Strategies for Trunk Disease of Grapevines in British Columbia Evaluation of the breadth and durability of engineered resistance to Plum pox virus based on the induction of RNA silencing Biological control of orchard insect pests Landscape ecology and genetics of invasive pests in orchards Identification of molecular markers linked to disease resistance through linakge analysis and association mapping approaches in durum wheat Genetic diversity and breeding use of dry bean (Phaseolus vulgaris L.) germplasm Novel molecular markers for wheat improvement 24 12 12 12 12 12 12 12 12 24 12 12 12 12 12 12 12 24 24 24 12-24 Page 2 of 78 24 12 24 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_Agassiz_01 Back PROJECT TITLE / TITRE DU PROJET : Improving Nutritional Quality of Organic and Hydroponic Tomatoes Internship location in Canada/ Pacific Agri-Food Research Centre, Internship Duration/Durée du Emplacement du stage Agassiz, British Columbia stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: david.ehret@agr.gc.ca d’AAC) David Ehret Phone/Téléphone: 1-604-796-1712 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agri-Food Research Centre Mailing address/Adresse postale du Centre : PO Box 1000, Agassiz, BC, Canada V0M 1A0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. David Ehret, Sustainable Production System Other AAFC collaborators/Autre collaborateurs à AAC: Drs. W. Lin, K. Usher, M. Cliff, T. Papadopoulos and M. Dorais University collaborators/Collaborateurs universitaires: Dr. A. Plant, Simon Fraser University, BC, Dr. E. Heuvelink, Wageningen University, the Netherlands Industry partners/Partenaires industriels: Gipaanda Greenhouses Ltd., BC. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Over the last 50 years, the nutritional quality of fruits and vegetables grown in North America has been shown to have declined. Despite this, consumer demand for nutritious vegetables is continually growing. Consumption of organically-grown foods is also at an all-time high. It is well-known that fruit and vegetable quality is influenced by growing conditions. The remarkable control of environmental conditions which is possible for greenhouse crops presents opportunities to significantly improve the nutritional quality of greenhouse vegetables. Objective/Objectifs: The goal of this project is to develop ways to improve the nutritional quality of greenhouse-grown tomatoes. The study will examine the effects of specific environmental growing conditions and short-term environmental stress on nutritional composition (vitamins, antioxidants and pigments), flavour, and shelf-life of hydroponic and organic greenhouse tomatoes. Outcome expected/Résultats attendus: Changes in fruit nutritional quality due to environmental conditions such as nutrient concentration, light, and water availability will be related to the underlying physiology, biochemistry and molecular biology. Subtle changes in whole-plant function such as growth rate and water uptake will be measured using novel automated monitoring technology and related to changes in fruit quality. Since organically-grown vegetables are an increasingly important part of the Canadian diet, organic fertilizer regimes will also being developed to promote higher nutritional quality in greenhouse-grown organic tomatoes. Finally, integrated fruit quality models will be developed to provide a better understanding of nutritional quality in relation to environmental growing conditions and the physiology of the crop. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Dr. Ehret has experience in training and mentoring Canadian and foreign students. The program will provide the candidate with a broad experience in technologically-advanced agricultural science and organic growing methods. Depending on the specific aspect selected for study, the candidate will learn techniques and concepts in horticulture (organic and hydroponic), plant and food chemistry, plant physiology, molecular biology, crop monitoring and modeling. The candidate will be given the opportunity to write scientific papers for international science journals. Candidate qualifications include some combination of knowledge and experience in horticulture, plant physiology, phytochemistry, food chemistry, molecular biology, statistics or computer modeling. (Update: January 28, 2009) Page 3 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_Agassiz_02 Back PROJECT TITLE / TITRE DU PROJET: Yield increase of corn following treatment of seed with Metarhizium anisopliae: the identification of causal factors Internship location in Canada/ Pacific Agri-Food Research Centre, Internship Duration/Durée du Emplacement du stage Agassiz, British Columbia stage: 12-24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: kabalukt@agr.gc.ca d’AAC) Todd Kabaluk Phone/Téléphone: 1-604-796-1710 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agri-Food Research Centre Mailing address/Adresse postale du Centre : PO Box 1000, Agassiz, BC, Canada V0M 1A0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Todd Kabaluk, Biologist Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Tom Forge, Research Scientist University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: Novozymes Biologicals, Inc. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: We have recently discovered that treating corn seed with conidia (spores) of the insect pathogenic fungus M. anisopliae resulted in an 11% increase in stand density and a 20% increase in area fresh weight yield based on field trials at three locations in 2006 (Kabaluk and Ericsson, 2007). The original purpose of the M. anisopliae corn seed treatments was to protect the corn plants from wireworm herbivory, as M. anisopliae is an insect fungal pathogen and has been developed in other countries for pest control. However, it is suspected that other factors are involved in causing the increase in corn yield. Based on the assessment of data to date, the following causes are considered for exploration: -wireworm mortality and subsequent protection of corn plants from wireworm herbivory -repellency of wireworms from M. anisopliae-coated corn seed, protecting the corn from herbivory i.e. M. anisopliae is simply by repelling them from germinating corn seedlings -competition of M. anisopliae with seed-rotting fungi such as Pythium and Fusarium, preventing loss of stand and improved plant health. -interaction of the M. anisopliae applications with plant pathogenic nematodes, conferring protection to the crop plant -colonization of corn rhizosphere with M. anisopliae, conferring an uptake of soil nutrients and improved emergence and plant health -M. anisopliae is digesting the corn seed coat, improving corn emergence and escape from wireworm herbivory Kabaluk, J. T. and Ericsson, J. D. 2007. Metarhizium anisopliae seed treatment increases yield of field corn when applied for wireworm control. Agronomy Journal. 99: 1377-1381. The yield-increase of corn as a result of treating seeds with M. anisopliae is a truly interesting and novel scientific phenomenon. Understanding the mode of action for the yield increase will represent a new contribution to science. Commercialization of M. anisopliae, either as a pest control or plant growth enhancement will undoubtedly take place following the research. Objective/Objectifs: The objective of this research is to understand why treating corn seed with M. anisopliae results in an increase in the yield of field corn. The understanding will come from the identification of the causal factor(s) of the yield increase listed under Background/Rational/Issue (above). This understanding is essential i) to make a meaningful contribution to science; ii) so that confidence is established with the public when this technology is commercialized; iii) so the scientific information can be further exploited to enhance the yield increase phenomenon; iii) to guide the use of the technology down a regulatory pathway associated with either pest control or plant growth enhancement. Outcome expected/Résultats attendus: The primary deliverable of this project is scientific information relating the causality of the yield increase in corn as a result of treating seeds with M. anisopliae. The information will be derived through scientific experimentation by the PDF, under the (Update: January 28, 2009) Page 4 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC guidance of Todd Kabaluk and Tom Forge, AAFC, and Jarrod Leland, Novozymes Biologicals. At the end of the project, the PDF will provide a summary of the work and its results to AAFC. Further research findings, as deliverables, will be assembled in the form of scientific manuscripts for publication in peer-reviewed journals. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. This project is an excellent opportunity for the prospective PDF to generate new scientific information to advance his/her scientific career aspirations to make a significant contribution to science. Discovering the mode of action for increased yield will be a significant contribution to science: both the use of microbial insecticides and recent discoveries on beneficial effects of certain insect-pathogenic fungi on plant growth are areas of interest in current science. The discovery and publication of the mode of action will enable other researchers and industry to investigate and exploit the knowledge for further improvements to agriculture and industry. The PDF will have his/her own office, laboratory space, and access to a technician and students for assistance. According to the needs of the project, equipment and apparatus will be available. We require that the PDF have experience, interest, or ability to work in at least two of the following areas: plant physiology, microbial ecology, entomology, entomopathology, microbiology. We require the PDF to be self-motivated and to possess a strong work ethic. (Update: January 28, 2009) Page 5 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_Fredericton_01 Back PROJECT TITLE / TITRE DU PROJET : Genomics-assisted potato breeding to meet global challenges Internship location in Canada/ Potato Research Centre, Internship Duration/Durée du Emplacement du stage Fredericton, New Brunswick stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: David.DeKoeyer@agr.gc.ca d’AAC) David De Koeyer Phone/Téléphone: 1-506-452-4885 Name of Research Centre/Nom du Centre de Recherche : AAFC-Potato Research Centre Mailing address/Adresse postale du Centre : 850 Lincoln Road, Fredericton, NB, CANADA E3B 4Z7 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. David De Koeyer, Research Scientist Other AAFC collaborators/Autre collaborateurs à AAC: Agnes Murphy, Helen Tai, Benoit Bizimungu, Larry Kawchuk University collaborators/Collaborateurs universitaires: Karen Tanino, University of Saskatchewan; Walter De Jong, Cornell University; Merideth Bonierbale, International Potato Center, Peru Industry partners/Partenaires industriels: Andrjez Killian, Diversity Arrays Technology Pty Ltd, Australia; John Argall, BioAtlantech B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: The potato is a key part of the global sustainable food system - the most significant vegetable crop in Canada and the world, producing more food energy on less land than corn, wheat or rice. More than half of total production is generated from developing countries, rendering it an important source of income to millions of farmers. Thus, the potato crop is a significant economic mechanism for the production of food to address intensifying health needs and the delivery of new bioproducts, such as bioplastics. To recognize the importance of the potato crop, the United Nations designated 2008 as the International Year of the Potato. Potato is susceptible to many pathogens and pests that result in economically significant disease losses globally. Global warming is expected to increase the full range of abiotic stresses as well as disease losses as pathogens and vectors expand their distributions. To develop ecologically and economically sustainable agricultural practices that reduce chemical and fossil fuel inputs, we must develop varieties with more durable and generalized resistance. Emerging marker technologies and statistical methods are required to access beneficial genetic variation related to stress tolerance from improved and unadapted germplasm and integrate them into breeding programs. Objective/Objectifs: To combine improved phenotypic screening methods with new transcriptome, metabolome and epigenome data for potato to identify candidate genes for abiotic and biotic stress resistance and novel bioproduct traits to contribute to marker development To generate natural allelic diversity information for marker development through targeted genome re-sequencing of candidate genes and genome regions. To identify and localize markers in the potato genome linked to economic and environmental traits using association genetics based on dosage-sensitive candidate gene and whole-genome marker profiling. To initiate Genomics-Assisted Breeding using markers for improving stress resistance and utilization traits in potato. Outcome expected/Résultats attendus: The proposed project will develop genomics tools for potato breeding programs with improved potato varieties for new and existing markets that can be grown with reduced inputs and reduced loss from abiotic and biotic stress. The project will integrate physiology, breeding and molecular approaches and produce a comprehensive Genomics-Assisted Breeding Platform. Key outcomes from this project will include: Identification of genes involved in abiotic stress resistance, disease resistance, and quality traits. DNA markers tightly linked to genes influencing important traits. Dosage sensitive markers for quantitative assessment of allele dosage in tetraploid potatoes. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. (Update: January 28, 2009) Page 6 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC The postdoc will be part of a comprehensive research team that will be developing genomics tools for potato improvement. The primary responsibilities of the candidate will be to develop high-resolution DNA melting (HRM) markers for candidate genes, to genotype a germplasm panel with candidate gene markers, to integrate HRM data with DArT genotyping data; and to conduct association genetics analysis. The expectation is that the candidate will have a doctoral degree in plant sciences, genetics, agriculture, or molecular biology; have experience in a molecular laboratory; have a good understanding of statistics; and have a willingness to learn new skills. The benefits to the intern will be conducting research within an applied genomics laboratory; working with a large multi-disciplinary team; having excellent opportunities for publication of novel research; and broadening skills in biotechnology and bioinformatics. (Update: January 28, 2009) Page 7 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Guelph_01 Back PROJECT TITLE / TITRE DU PROJET : Antioxidant properties and potential health benefits of pigmented vegetables Internship location in Canada/ Guelph Food Research Centre, Internship Duration/Durée du Emplacement du stage Guelph, Ontario stage: 12 months/mois Contact : Rong Cao (R. Tsao) Email/Courriel: caor@agr.gc.ca Phone/Téléphone: 1-519-827-0550 Name of Research Centre/Nom du Centre de Recherche : AAFC-Guelph Food Research Centre Mailing address/Adresse postale du Centre : 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Rong Cao, Functional Foods & Nutraceuticals Team Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Dr. Mary-Ruth McDonald Industry partners/Partenaires industriels: OMAFRA B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Consumption of fruits and vegetables has been related to significant reduction of chronic diseases, particularly cancer. This is one of the five health claims approved by Health Canada. Phytochemicals are now considered to play a major role in the prevention of cancer and cardiovascular diseases. Such physiological properties of vegetables have been attributed to the strong antioxidant capacities of the phytochemicals instead of the essential vitamins such as vitamin C. Although in general polyphenols and carotenoids are known as the major antioxidative phytochemicals, and compounds such as anthocyanins are well know to be the major contributor to the antioxidant capacity of small berries e.g. blueberries and their various health benefits, knowledge and information on the phytochemical profiles of different vegetables and factors affecting the phytochemicals (genetics, environment, post-harvest and processing conditions etc) are largely unknown. On the other hand, efforts in producing high antioxidant vegetables have been made. In the Province of Ontario, highly pigmented vegetables with yellow, orange, red, blue and purple colours are being examined currently for their anthocyanin and carotenoid compositions, and the effects of cultivar, geographic location and fertilizers. There is a need to develop validated method for phytochemicals in such new vegetables, and examine their antioxidant capacities. Objective/Objectifs: 1. To modify and develop method for the analysis of phytochemicals in most economically important vegetables in Ontario : tomato, potato, cauliflower, cabbage, carrot, onion, broccoli 2. To further investigate the anthocyanin and carotenoid compositions and factors affecting of pigmented varieties (variety, location, nitrogen, processing etc) 3. To investigate the antioxidant capacities using in vitro methods Outcome expected/Résultats attendus: 1. 2. 3. 4. Phytochemical database of major Ontario vegetable crops such as purple potatoes, purple cauliflowers, purple carrots. Cultivars and best agronomic practice and processing conditions for maximized antioxidant capacities of Ontario vegetables Information for consumers on the potential health benefits of the new and pigmented Ontario vegetables Increased production and income by Ontario Vegetables farmers C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The program is to study highly pigmented vegetables and understand their health benefit (antioxidant potential). The intern should be a PhD preferably in food /natural products/analytical chemistry with experiences in phytochemicals and antioxidants research. The intern will be trained in a chemistry laboratory setting and will benefit from the following techniques including : extraction of phytochemcials from different vegetables ; sample clean up and pre-concentration techniques such as solid phase (Update: January 28, 2009) Page 8 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC extraction (SPE) ; analysis of total phenolic content using Folin-Ciocalteu method ; total anthocyanin/carotenoid content using spectrophotometric detection ; analysis of phenolic compounds and carotenoids using HPLC (high performance liquic chromatography) ; HPLC-MS (mass spectrometry), in vitro antioxidant capacity measurement using FRAP, ORAC, PCL, betaCLAMS, and cell-based antioxidant activity method (CAA). (Update: January 28, 2009) Page 9 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Guelph_02 Back PROJECT TITLE / TITRE DU PROJET: ‘Green’ extraction process to produce functional food ingredients from agricultural material Internship location in Canada/ Guelph Food Research Centre Internship Duration/Durée du Emplacement du stage Guelph, Ontario stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: shij@agr.gc.ca d’AAC) John Shi Phone/Téléphone: 1-519-780-8035 Name of Research Centre/Nom du Centre de Recherche: AAFC-Guelph Food Research Centre Mailing address/Adresse postale du Centre : 93 Stone Road West, Guelph, Ontario N1G 5C9 Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr John Shi, Functional Foods & Nutraceuticals Team Other AAFC collaborators/Autre collaborateurs à AAC: Dr Elsayed Abdelaal, Dr Magda Kostrzynska, Dr Dan Ramdath, University collaborators/Collaborateurs universitaires: University of Guelph (Dr Yukio Kakuda, Dr Gauri Mittal); Industry partners/Partenaires industriels: Heinz Canada Co.; Joseph Natural Products, Ltd. B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: The development of separation/ extraction/ concentration processes to produce high value-added functional food ingredients from agricultural materials are now being recognized as one of the most important technological needs by agri-food industries. The challenge is to develop “organic-solvent free” “green” extraction technologies to meet food safety regulations and to economically preserve the bioactivity of health-promoting compounds. Supercritical fluid-based extraction (SFE) processes have drawn much attention by extracting high-value compounds and opening new opportunities for innovative food products. To achieve the goal for functional foods and ingredients that are free from chemical residues, an “organic-solvent free” “green” separation/extraction process by supercritical-CO2 fluid –based technology, must be developed, optimized, and fully exploited to produce the functional foods demanded by consumers. AAFC has considered development of functional foods by novel separation technology as priority research area, and given full support in research activity. Dr. Shi and his research team have launched projects related to extracting lycopene from tomatoes, β-carotene from pumpkin, sweet potatoes, and some berry fruits, etc. He established collaborations with Heinz Co., Joseph Natural Products. Separation equipment such as supercritical CO2 fluid extractors, microwave solvent extractor, molecular distillation separation apparatus, and membrane separation equipment is available in Guelph. Advanced analysis facilities such as HPLC, GC, LC-Mass, GC-Mass, chemiluminescence meter, etc. are also available. This team at AAFC has internationally recognized scientific expertise, is world class with broad experience, international reputations. Objective/Objectifs: The Internship project is “Development of ‘organic-solvent free’ ‘green’ extraction processes by supercritical fluid-based technologies to produce health-promoting functional food ingredients from agricultural plant material”: (a). development of an optimum supercritical fluid extraction to provide high selectivity, solubility and yield, bioactivity stability; (b) development of modeling methods to monitor the extraction process and product quality and bioactivity degradation of health-promoting food ingredients; Outcome expected/Résultats attendus: The proposed project is to develop a new technology that will enhance the extraction of health-promoting components such as carotenoid-rich functional food ingredients. It would be highly interested for the researcher to develop natural high-value-added carotenoids-rich functional food ingredients for people health, and have high benefits for economic development. Dr Shi has some classmates working as professors in universities. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. (Update: January 28, 2009) Page 10 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Internship project: “Green” extraction process to produce functional food ingredients from agricultural material” Methods: 1).Design of process conditions to extract carotenoids from selected carotenoid-rich materials by using SFE-CO2 equipment, 2). Modifiers (Co-solvents) design: water, ethanol, or food-grade-oils are used as co-solvents. 3).Optimization of operation parameters: to establish an optimized operation condition, experiments will be conducted to determine the effect of extraction parameters and conditions such as pressure, temperature, moisture level, particle size, CO 2 flow rate, co-solvent, etc. on extraction yields and selectivity of the targeted component. Work Plan and Outputs: a) to prepare experimental plan (1st month), b) to optimize operating condition for extracting selected materials(2-3th months), c) to study effects of pre-treatments on yield of extracted products (4-5th month), d) to determine solubility, mass transfer coefficients, and other engineering properties of extracts under different conditions (610th month), e) to determine stability of bioactivity of caroteniod-rich functional foods under different conditions (9-11th month), f) to develop optimal process condition and process modeling (9-12th month), Post-doctoral fellow with background of food engineering, chemical engineering, food science and technology will well fit in this project. They will be well –trained through the lab work activity and to gain sound knowledge and skill. (Update: January 28, 2009) Page 11 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Guelph_03 Back PROJECT TITLE / TITRE DU PROJET : Genotype by environment interaction and mega-environment investigation in long term common bean (Phaseolus vulgaris L.) regional variety testing in Ontario Internship location in Canada/ Universtiy of Guelph, Internship Duration/Durée du Emplacement du stage Guelph, Ontario stage: 12 months/mois Contact : Alireza Navabi Email/Courriel: alireza.navabi@agr.gc.ca Phone/Téléphone: 1-519-824-4120 ext. 56829 Name of Research Centre/Nom du Centre de Recherche : AAFC-Greenhouse and Processing Crops Research Centre Mailing address/Adresse postale du Centre : 2585 County Road 20, Harrow, ON, Canada N0R 1G0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Alireza Navabi, Research Scientist , Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Dr. Istvan Rajcan, Universtiy of Guelph Industry partners/Partenaires industriels: Ontario White and Colored Bean Growers Associations B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Multi-location and regional variety performance trials of common bean (Phaseolus vulgaris L.) in Ontario is performed annually, in order to identify superior genotypes for commercial release and to provide growers with variety recommendations. Like other multi-location variety trials, results of these tests are generally confounded by the presence of crossover genotype by environment interaction, which complicates the process of variety selection and recommendation. In this research, we intend to use long-term variety trial data from Ontario, along with agro-climatic data and to apply novel statistical models to test the hypothesis that bean growing areas in Ontario may be dissected to subsets of regions with repeatable crossover interaction. This may lead to identification of bean growing mega-environments in Ontario. We will also attempt to investigate if the identified mega-environment(s) can be separated by agro-climatic indicators such as cumulative crop heat units during the growing season, soil type etc. Objective/Objectifs: 1) To study the genotype by environment interaction patterns in multi-location trials and 2) To perform mega-environment investigations using a long term dataset of common bean variety trials in Ontario. Outcome expected/Résultats attendus: Identification of bean growing mega-environments in Ontario and identification of sites that have historically been more informative of the genotype by environment interaction patterns of bean genotypes in Ontario. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The intern will have the opportunity to work with the AAFC Bean Breeding Program at the University of Guelph in Guelph, Ontario. The program is currently working on a variety of objectives, including breeding for high yield, high quality, resistance to major bean diseases and the canopy structure suitable for direct combining. For the purpose of this research, a dataset of long-term variety trials in Ontario is being put together. The internship program will provide the PDF with experience in application of novel statistical models in plant breeding data analysis. For this position, prior training in plant breeding, knowledge of advanced statistics, genotype by environment interaction analyses and SAS programming is required. The PDF will have the opportunity to learn a range of novel approaches in statistical analysis of multi-location data. The intern may also bring along data from crop breeding programs of the home country and perform similar tests on a dataset from his/her country. It is expected that this research result in at least one scientific publication in peer-reviewed journals. (Update: January 28, 2009) Page 12 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Harrow_01 Back PROJECT TITLE / TITRE DU PROJET : Coarse mapping of an F2 bean population BBSS x Jalo EEP558 by Diversity Arrays Technology (DArT) Internship location in Canada/ Greenhouse and Processing Crops Research Centre, Internship Duration/Durée du Emplacement du stage Harrow, Ontario stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: yuk@agr.gc.ca d’AAC) Kangfu Yu Phone/Téléphone: 1-519-738-1207 Name of Research Centre/Nom du Centre de Recherche : AAFC-Greenhouse and Processing Crops Research Centre Mailing address/Adresse postale du Centre : 2585 County Road 20, Harrow, ON, Canada N0R 1G0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Kangfu Yu, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Drs : Navabi, Alireza; Marcus Shi University collaborators/Collaborateurs universitaires: Drs : William B. Crosby; Peter Pauls Industry partners/Partenaires industriels: Ontario White and Colored Bean Growers Associations B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Common bacterial blight (CBB) of common bean is a significant bacterial pathogen that is endemic to the $280 Million annual processing bean production industry in Canada. At present, cultivation of CBB resistance cultivars is the only effective and environmentally sound approach to management of this disease in bean production. Application of marker-assisted selection (MAS) can significantly increase the selection efficiency in bean breeding program. However, the whole bean genome is not sequenced yet and the high-throughput genotyping method, Diversity Arrays Technology (DArT), is also not established in bean. DArT is a novel large-scale genotyping technique that has been very attractive to breeders due to its low cost and high throughput, as well as its ability to generate reproducible molecular markers with no prior DNA sequence information. Objectives/Objectifs: 1) To establish the DArT technology in common bean genetics; 2) To apply DArT for high-throughput genotyping and linkage mapping of an F2 population derived from the BBSS (resistant) x Jalo EEP558 (susceptible) cross. Outcomes expected/Résultats attendus: 1) To produce the Bean Diversity Array containing ≥1,500 DArT markers; 2) To genotype 52 lines including two parent lines and 50 individual F2 lines; 3) To construct a coarse map of the F2 BBSS x Jalo EEP558 population with ≥100 markers. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The internship program will focus on preparing genomic representations from Jalo EEP558 and generating the Bean Diversity Array. For this position, prior training in plant genetics and plant molecular biology is preferred. Experience with plant pathology will also be a plus. The internship will have the opportunity to learn a range of cutting-edge genomics techniques, such as, enriched DNA library construction, macroarray-based hybridization, and microarray data analysis. Upon completion of this training program, the intern could adapt these technologies to improve bean production in the home country. (Update: January 28, 2009) Page 13 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Harrow_02 Back PROJECT TITLE / TITRE DU PROJET : Evaluation of bumble bees as vectors for microbial control agents (viruses and fungi) for arthropod pest management Internship location in Canada/ Greenhouse and Processing Crops Research Centre, Internship Duration/Durée du Emplacement du stage Harrow, Ontario stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: shippl@agr.gc.ca d’AAC) Les Shipp Phone/Téléphone: 1-519-738-1235 Name of Research Centre/Nom du Centre de Recherche : AAFC-Greenhouse and Processing Crops Research Centre Mailing address/Adresse postale du Centre : 2585 County Rd. 20, Harrow, Ontario, Canada N0R 1G0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Les Shipp, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Bruce Broadbent (London) and Martin Erlandson (Saskatoon) University collaborators/Collaborateurs universitaires: Dr. Peter Kevan, University of Guelph Industry partners/Partenaires industriels: Biobest Canada, Novozymes Biologicals Inc. and NSERC Canadian Pollination Initiative Network B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Traditionally, microbial control agents have been applied to crops for pest control using standard chemical spray application technology. It has been known for a long time of the ability of bees to carry microscopic particles such as pollen. Dr. Shipp and colleagues have developed a “Pollinator Biocontrol Vector Technology” for using bee pollinators to deliver fungal control agents for insect pest control and plant disease suppression using the agents, Beauveria bassiana and Chlonostachys rosea. Greenhouse cage trials have shown that bee-vectored B. bassiana can cause substantial mortality of Lygus, whiteflies, thrips and aphids (up to 80% mortality) when tested on greenhouse tomato and sweet pepper. The bees deliver the fungal spores directly to the flowers and leaves where the pests are found. This approach reduces the amount microbial agent applied, minimizes human handling of the fungal control agents, eliminates the use of pesticides for insect control and is more environmentally-friendly and cost effective than the application of chemical pesticides. Since bumble bees are the standard pollination method in greenhouse vegetables, you can combine pollination and pest control activities together. In addition, preliminary greenhouse cage trials have found that bumble bees can simultaneously deliver both a pest fungal biocontrol agent (B. bassiana) and a plant inoculum (C. rosea) to sweet pepper for Lygus (ca. 80% infection) and grey mould (45-55% suppression) control. The impact of these fungal agents on bumble bees appears to be minimal. The next steps in developing this technology is to evaluate the ability of bumble bees to deliver insect viruses for pest control and assess the ability of bees to vector other entomopathogenic fungi, such as Metarhizium anisopliae. This project involves a multidisciplinary team approach consisting of AAFC, a Canadian university and industry partners. The overall objective of this project is to develop an effective, environmentally-friendly method for application of microbial control agents using bee pollinators for pest control of greenhouse vegetable crops. The end result will be safer food products for the consumer through the elimination of insecticides. Objective/Objectifs: 1. Evaluate the effectiveness of using bumble bee pollinators for delivery of the entomopathogenic fungus, M. anisopliae, for pest control. 2. Evaluate the use of bumble bees as a delivery system for baculoviruses for control of lepidopteran pests. Outcome expected/Résultats attendus: Determination of the ability of the commercially-reared bumble bee, Bombus impatiens, as a new application method for delivering M. anisopliae for pest control to crops. Development of a new methodology for the application of insect viruses to crops for pest control. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / (Update: January 28, 2009) Page 14 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship Program: Intern will assist in conducting greenhouse cage trials: to determine the optimal bee-vectored concentration ratio of M. anisopliae for pest control (whitefly, thrips and aphids) on greenhouse tomato and sweet pepper to determine the impact of bee vectored M. anisopliae on non-targets hosts (ie, bumble bees and biological control agents) to determine the amount of M. anisopliae deposited on plant structures (flowers and leaves) to determine the effectiveness of using bee vectoring to deliver baculoviruses to crops for control of lepidopteran pests (ie. cabbage looper) to improve the design of the inoculum dispenser. Expected qualifications of the intern: Experience in entomology and integrated pest management. Knowledge of greenhouse pests, bumble bees and insect pathology is an asset, but not essential. Benefits to the intern: Gain experience with a new novel IPM strategy of using pollinators to deliver microbial control agents for control arthropod pests and diseases in greenhouse crops. Gain experience in IPM in Canadian greenhouse vegetable crop production systems. The main emphasis of greenhouse vegetable IPM in Canada is biological control. Be part of a greenhouse IPM/pollination team that is recognized as a world leader in this area of research. Broaden their network of contacts in entomology and pest management. (Update: January 28, 2009) Page 15 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID: 2009_Harrow_03 Back PROJECT TITLE / TITRE DU PROJET : EcoTILLING Molecular analysis of glycinin genes in soybean (Glycine max) mutant lines. Internship location in Canada/ Greenhouse and Processing Crops Research Centre, Internship Duration/Durée du Emplacement du stage Harrow, Ontario stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: poysav@agr.gc.ca d’AAC) Vaino Poysa Phone/Téléphone: 1-519-738-1260 Name of Research Centre/Nom du Centre de Recherche : AAFC-Greenhouse and Processing Crops Research Centre Mailing address/Adresse postale du Centre : 2585 County Rd. 20, Harrow, Ontario, Canada N0R 1G0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Vaino Poysa, Research Scientist – Soybean Breeding and Genetics Other AAFC collaborators/Autre collaborateurs à AAC: Drs. K. Yu & S. Jegadeesan University collaborators/Collaborateurs universitaires: Dr. Peter Pauls, U of Guelph Industry partners/Partenaires industriels: Ontario Soybean Growers B – Project Description: rational, objectives, outcome expected/Description du projet: rationnel, objectifs, résultats attendus Background, Rationale, Issue / Context, Rationale, Problématique: Soy food quality is determined largely by soy seed protein content and composition. Together glycinin (11S) and β-conglycinin (7S) proteins account for about 70% of the total soybean seed proteins, with glycinin accounting for about 40%. Functional properties of soy proteins, such as gelation and emulsification, are determined by the total and relative amounts of the different 11S and 7S protein subunits or peptides. Since glycinin plays such an important role in the properties of foods made from soybean, the soy breeding program at Harrow has developed lines with different protein subunit compositions. Understanding the genetic control for the presence or absence of a specific protein subunit would help us to develop soybean varieties with optimal combinations of the protein subunits. Glycinin has five subunits, coded for by five genes, divided into group-1(Gy1, Gy2 and Gy3) and group-2 (Gy4 and Gy5). We have shown that the absence of the A4 peptide is conditioned by a single recessive null allele resulting from one base pair mutation in the Gy4 gene. The absence of the A4 peptide has been associated with superior nigari tofu quality, while the absence of A4 and 7S ά results in superior GDL tofu quality. We have developed a series of unique soy lines with various protein subunit compositions, including lines lacking all 11S subunits and the 7S ά subunit, as well as various combinations of the 11S subunits. The availability of the complete soy genome sequence combined with reverse genetics will facilitate the identification of novel genetic variations in any gene affecting the phenotype. Targeting Induced Local Lesions IN Genomes (TILLING) and EcoTILLING techniques are being used to discover polymorphisms in specifics gene in mutated and natural populations respectively, providing guidelines to develop new strategies for genetic improvement of soybean. Objective/Objectifs: (1) To study genetic variation behind different glycinin subunit mutant lines. (2) To understand whether INDELs of structural genes or mutations of regulatory genes are involved in regulating expression of glycinin genes. (3) To associate genetic variation (SNPs and INDELs) with protein subunit polymorphisms contributing to improved soy food quality. Outcome expected/Résultats attendus: New SNPs and other genetic variations identified in this study could be used as genetic markers in breeding programs for quality improvement. Understanding the genetic control of soy protein subunits will allow breeders to combine specific protein subunits to develop superior soybean cultivars for soy food production. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The internship at Harrow will be integrated into the soy breeding and molecular genetics labs, providing the PDF with an opportunity to learn new techniques and improve their skills in breeding and molecular genetics. The candidate should have a Ph.D. in molecular biology, plant breeding, plant biochemistry, plant biology or in a related field. The selected applicant will be involved in the identification of SNPs and other genetic polymorphisms in soybean, cloning the different protein subunit polymorphic gene(s), and sequencing the genes. The PDF will also assist in associating the polymorphism with protein quality. The PDF will have the opportunity to learn more about Canadian agriculture and to observe a wide range of research activities in a progressive AAFC Research Center. (Update: January 28, 2009) Page 16 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Kentville_01 Back PROJECT TITLE / TITRE DU PROJET: Manipulation of intestinal bacterial communities; an investigation into the impact of dietary fibre and prebiotics. Internship location in Canada/ Atlantic Food and Horticulture Research Centre, Internship Duration/Durée du Emplacement du stage Kentville, Nova Scotia stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: KalmokoffM@agr.gc.ca d’AAC) KALMOKOFF, Martin Phone/Téléphone: 1-902-679-5374 Name of Research Centre/Nom du Centre de Recherche : AAFC-Atlantic Food and Horticulture Research Centre Mailing address/Adresse postale du Centre : 32 Main St., Kentville, Nova Scotia, Canada B4N 1J5 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Martin Kalmokoff (Research Scientist), Food Safety and Quality Other AAFC collaborators/Autre collaborateurs à AAC: Steve Brooks (Acting Chief, Bureau of Nutritional Research, Food Directorate, Health Canada, Ottawa) University collaborators/Collaborateurs universitaires: Julia Green-Johnson (Professor Immunology, University of Ontario Technical Institute), Brent Selinger (Professor Microbiology, University of Lethbridge) Industry partners/Partenaires industriels: Advanced Food and Materials Network (AFMnet) B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Emerging evidence indicates that the bacterial community associated with the gastro-intestinal tract plays an important role in the health status of the host. Over the last decade there has been significant market growth in food products and ingredients having health claims pertaining specifically to gut-health. These products represent a source for significant additional economic value from the commodity food chain. However, health related claims require regulatory approval and it has been recognized that current regulatory framework is inadequate to assess gut-health claims associated with these new and novel food products. Furthermore, current approaches used by industry to justify health claims lack a firm scientific basis. Traditional approaches for the analyses of gastro-intestinal communities are based on cultivation. Not surprisingly, many foods having purported gut-health promoting properties base their health claims on the ability of these substrates to alter the numbers of certain readily cultivable bacterial genera as proof of positive impacts on gut health. However, it is now recognized that the vast majority of colonic bacteria have yet to be cultivated, and that cultivation alone provides a very limited and skewed perspective on change within these microbiologically complex communities. Over the last five years molecular based approaches have largely superseded cultivation-based community analysis. While these new phylogenetics-based approaches have provided new insights into the complexity and composition of the communities found in a wide variety of animals, these studies remain largely descriptive. Objective/Objectifs: There are few studies which have used molecular based methods to analyze gut-community change in response to dietary fibre, prebiotics or probiotics. However there is a need for functional-based studies so that change within the colonic flora may ultimately be correlated with other indicators of gut-health. Our objectives are to understand how different dietary fibre sources alter the colonic community and impact the immunological status of the host. Outcome expected/Résultats attendus: Data generated from different dietary fibre sources using a single animal model will establish a baseline useful to assess the properties of new and novel functional fibre sources. Understanding how dietary fibre alters colonic communities will allow a more realistic basis to assess health claims and begin to correlate improvements in gut health with colonic community composition. This data will be used by regulatory agencies to assess functional claims and by industry to identify and characterization the activities of new functional dietary fiber sources. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The PDF will participate in an ongoing national collaboration aimed at elucidating the impact of different dietary fibre sources on colonic community composition and the impact on the immunological state of the host using a rat nutrition model. It would be advantageous for the PDF to have some previous experience in microbiology or molecular biology, although this is not an (Update: January 28, 2009) Page 17 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC absolute requirement as we are willing to provide training. The PDF will participate in on-going animal feeding trials involving various dietary fibre sources and interact with scientists and technicians at various locales in Canada. Change within the colonic community in response to these fibre sources will be assessed using both cultivation dependent (anaerobic cultivation) and cultivation-independent methods (16S rRNA libraries, DGGE). The PDF will gain experience in current molecular methodologies useful for the analysis of complex microbial communities, where ever they may originate from. Within the context of this main project are additional opportunities to carry out basic and applied research on a number of related topics. Examples of this include studies on the characterization of new anaerobic bacteria, studies on structure/function in anaerobic bacteria, metagenomic-based analysis of colonic communities and the genetic and physical characterization of new fibre hydrolytic enzymes. (Update: January 28, 2009) Page 18 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Lethbridge_01 Back PROJECT TITLE / TITRE DU PROJET : Identification and selection of potato clones with novel starch properties for food and industrial uses Internship location in Canada/ Lethbridge Research Centre, Internship Duration/Durée du Emplacement du stage Lethbridge, Alberta stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: BizimunguB@agr.gc.ca d’AAC) Benoît Bizimungu Phone/Téléphone: 1-403-317-2276 Name of Research Centre/Nom du Centre de Recherche : AAFC-Lethbridge Research Centre Mailing address/Adresse postale du Centre : 5403- 1 Avenue S. P.O Box 3000, Lethbridge AB T1J 4B1 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Benoît Bizimungu, Research Scientist (potato breeding) Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Qiang Liu (Guelph) and Xiu-Qing Li (Fredericton) University collaborators/Collaborateurs universitaires: Prof. Alan Sullivan and Prof. Ming Fan (U.Guelph), and Prof. Thomas Wolever (U. Toronto) Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique : Potato has recently risen to the third rank among the most important food crops worldwide, with more than half of total production generated from developing countries. To recognize the importance of the potato crop, the United Nations had designated 2008 as the International Year of the potato. Potato is also a high value crop in Canada, and one of the most important sources of dietary starch for many Canadian diets. Potatoes account for about 39 per cent of all fresh and processed vegetables consumed in Canada, representing a total annual consumption of about 72.69 kg per capita. The newly ABIP funded BioPotato Network seeks to deliver bioproducts that address key issues concerning human and environmental health, including the development of a low glycemic index potato, functional foods, and industrial starch. The production of new potato germplasm high in slowly digestible or resistant starches and fibre content, tailored for the food sector which have proven health benefits to the consumer, would be beneficial to the health and well-being of Canadians and increase the markets of Canadian-based food industries and producers. Objective/Objectifs: To develop new screening tools (including NIR spectrometry and/or molecular markers) for use in potato breeding to develop germplasm with novel starch properties for food and industrial applications. Outcome expected/Résultats attendus: Improved potato germplasm with low glycemic index, germplasm with novel starch properties for food or industrial applications, new screening tools for the development of germplasm with altered starch characteristics. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The candidate will be expected to participate in the screening and the development of potato germplasm with altered starch characteristics for food and industrial uses, using NIR spectrometry and/or molecular markers. Ph.D. degree specializing in plant/food science and familiarity with starch analysis and molecular markers are desirable. (Update: January 28, 2009) Page 19 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_Lethbridge_02 Back PROJECT TITLE / TITRE DU PROJET : Genetic engineering of cereal crops to produce novel starch Internship location in Canada Lethbridge Research Centre, Internship Duration/Durée du Emplacement du stage Lethbridge, Alberta stage: 12 -24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: luj@agr.gc.ca d’AAC) John Lu Phone/Téléphone: 1-403-381-0118 Name of Research Centre/Nom du Centre de Recherche : AAFC-Lethbridge Research Centre Mailing address/Adresse postale du Centre : 5403 - 1st Ave. S. Lethbridge, AB T1J 4B1 Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. John Lu, Bio-Products and Bio-Processes Other AAFC collaborators/Autre collaborateurs à AAC: Drs. A. Laroche, D. Gaudet, F. Eudes University collaborators/Collaborateurs universitaires: University of Alberta, University of Lethbridge Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Cereal crops represent one of the most important economic forces over the world. Scientists at Lethbridge Research Centre have been working on improvement of wheat yield and quality for many years and already made significant contributions to wheat production in Canada. Starch is the most abundant and renewable polysaccharide in cereal grains and can be produced cost-effectively in vast quantities by using modern agronomic systems. Genetic modifications of cereal genotypes create a new platform to produce value-added starches for carbohydrate-based industrial applications. Objective/Objectifs: Our research objective will be focused on genetic modifications of wheat and triticale genotypes to produce value-added starches, such as amylose-free starches (waxy starches) and high-amylose starches (resistant starches), for industrial applications. Outcome expected/Résultats attendus: This research project will turn out measurable and deliverable bioproducts (e.g. waxy and resistant starches) and biotechnology (e.g. novel delivery system for genetic modification). Such outcomes can benefit the economy and also advance our knowledge in plant sciences. C –Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The post-doctor under this internship program will have the opportunity to employ the latest techniques for studying gene isolation, expression, transient and whole plant transformation, quantitative real-time PCR, RNAi, and all other ancillary biotechnologies. He/she will use these techniques to isolate and characterize the conserved domains of cereal starch syntheses genes, to construct the expression vectors for RNAi function, to conduct the target gene transformations, to develop transgenic wheat and triticale genotypes, and to evaluate the transformants for determining novel starch properties. The post-doctor will be trained to conceive research objectives, design experimental approaches, conduct the independent researches, and analyze the experimental results. He/she is required to have strong communication skills on speaking, reading, and writing in English, and would be expected in preparation and presentation of experimental results at local or national conference and in preparation of a manuscript on the work carried out in our labs. (Update: January 28, 2009) Page 20 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Lethbridge_03 Back PROJECT TITLE / TITRE DU PROJET : Assessment of molecular and genetic diversity in specific bacterial population from beef cattle - Implications for the Food safety Internship location in Canada/ Lethbridge Research Centre, Internship Duration/Durée du Emplacement du stage Lethbridge, Alberta stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: sharmar@agr.gc.ca Phone/Téléphone: 1-403-317-2232 d’AAC) Ranjana Sharma Name of Research Centre/Nom du Centre de Recherche : AAFC-Lethbridge Research Centre Mailing address/Adresse postale du Centre : 5403-1Ave. S, PO BOX 3000, Lethbridge, AB T1J 4B1, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Ranjana Sharma, Biotechnologist, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Ed Topp, Tim McAllister, University collaborators/Collaborateurs universitaires: Drs. L. Brent Selinger, James Thomas, Marie Louie, Ron Read (located at Universities of Lethbridge and Calgary). Industry partners/Partenaires industriels: awaiting confirmation B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: The proposed research project is part of initiative of Agriculture and Agri-Food Canada (AAFC) aimed at assessing the sustainability of current farming practices for livestock production and factors that enhance the food safety. The results from the research will be of direct consequence to safe agri-food productions and at finding sustainable beef production practices that are ecologically and environmentally sustainable. The proposed project will be focussed on Assessment of molecular and genetic diversity in specific bacterial population from beef cattle – and has Implications for the Food safety. The proposed research will address the impact of production environment and practices on the bacterial populations. The research will use the bacterial isolates being currently collected from an ongoing animal trial at AAFC, Lethbridge. Objective/Objectifs: To quantify the specific bacterial population from the feces and rumen digesta samples from animals. Specific objectives : 1) quantify specific bacterial populations from animal feces destined for the food chain using Real-Time PCR. Correlate the quantification between samples derived from rumen vs. feces to understand the stability of specific population. 2) assess present role of diets and farming practices in affecting beneficial bacteria and identifying factors and/or conditions that supports their growth. Outcome expected/Résultats attendus: The research will provide an integrated knowledge of beef production system with regards to specific bacterial quantification and persistence in the food chain. These results will be useful in devicing food safety practices. The methods developed in the study will provide diagnostic tools to detect, monitor and control bacterial related hazards in the food chain and environment. The results from the study will provide data to better manage on-farm spread of specific bacteria. There is a need to establish the prevalent methods of spread of antibiotic-resistant (AR) bacteria before we can develop and plan for mitigation strategies. The results will identify ways to limit and and contain AR in food production. The results will be applicable to agricultural industry since it has agricultural production base (meat and fish). The molecular diagnostic techniques used in the proposed study are versatile and are applicable for addressing related food safety concerns. Relevance to AAFC strategic priorities: This proposal is closely aligned with two of the AAFC research priorities; namely “Enhancing environmental performance of the Canadian agricultural system” and “Enhancing the quality of food and the safety of the food system”. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship Program: The selected individual will be involved in molecular research using fecal samples derived from (Update: January 28, 2009) Page 21 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC animals. He/She would work in the laboratory of the PI with the aim of addressing the proposed objectives. The individual will conduct experiments, compile and analyze data and will work on writing and presenting results for scientific audience. Expected Student Qualification: The candidate must either have a Ph. D degree or in the final phase of completion of the degree. Trained in Microbiology and Molecular Biology with knowledge of DNA/RNA extraction, PCR and Real-time PCR analysis. Benefits to the student: The individual will add to his/her abilities and skills in terms of experimental procedures and techniques. He/She will get training in molecular fingerprinting and molecular quantitative techniques for specific bacteria. These techniques are versatile in their application and can be applied for diagnostics and food safety issues such as bacterial tracking through food chain. Application of these techniques will help to understand the critical mitigation points and the measures required for safe food supply and reducing environmental pollution. Apart from the technical skills, the individual will get adept with the scientific writing. Hence the work term will be highly beneficial to the incumbent. (Update: January 28, 2009) Page 22 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Lethbridge_04 Back PROJECT TITLE / TITRE DU PROJET : Develop alternative growth promoter in ruminant production Internship location in Canada/ Lethbridge Research Centre, Internship Duration/Durée du Emplacement du stage Lethbridge, Alberta stage: 12 months/mois Contact: Wenzhu Yang Email/Courriel: yangw@agr.gc.ca Phone/Téléphone: 1-403-317-3427 Name of Research Centre/Nom du Centre de Recherche : Lethbridge Research Centre Mailing address/Adresse postale du Centre : 5403-1 Ave. S. Box 3000, Lethbridge, AB, Canada T1J4B1 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Wenzhu Yang, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Maolong He University collaborators/Collaborateurs universitaires: Dr. Burim Ametaj, University of Alberta Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected/Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Rare earth elements (REE) including La, Ce and other lanthanides are a group of elements with many similarities in chemical and biochemical characters. The REE have been used as feed additives in animal production for more than twenty years in China, and have been recently shown, in Europe, improved growth performance of pigs, poultry and calves. In Canada, we are the first to have conducted the studies using the REE in animal nutrition. Our results indicated that supplementation of REE in media culture stimulated proliferation of 3T3-L1 preadipocytes, and affected the fatty acid composition in lipids accumulated in the cells. Supplementation of REE in the diet of feedlot cattle increased dry matter disappearance in vitro that was resulted mainly from the increased of digestibility of fibre. Including REE in the culture medium also stimulated ConA-induced splenocyte proliferation. The finding suggests a dietary REE-mediated enhancement of immunological function that may contribute to improved immune response and possibly prevent from disease. Given their potential but very limited information on their mode of action in animal, these elements should be considered for a series of examination in vitro or in vivo to understand their potential activities. Objective/Objectifs: to elucidate the mode of action of REE under continuous flow rumen environment or in animal, and to define the optimum dosage of REE that could be safely used under feedlot cattle management system. Outcome expected/Résultats attendus: The expected results of this project are to enhance the efficiency of feed utilization, lower the cost of production, ameliorate immune status and animal health, and improve animal welfare. Another important outcome of the proposed research is to decrease feedlot industry reliance on in-feed antimicrobials, lower the risk of antibiotic resistance and its consequences to human health. The outcomes of the proposed work will have significant implications on the sustainability of dairy and beef cattle production systems. The proposed research project is part of a larger and longer research program aiming at developing nutraceuticals as new alternatives to in-feed antibiotics for use in livestock production systems. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The potential candidate will become a member of our integrated research team and is expected to have an in-depth knowledge of biochemistry, ruminant nutrition, and immunology. More specifically, the student will be expected to: 1) use in vitro, in vivo and molecular (Real-time PCR) techniques as research tools to assess the effects of REE on rumen bacterial composition and to define the optimum conditions for application of these novel additives; 2) conduct in vivo studies to assess the effects of REE on rumen fermentation, animal performance and modulation of immune status; 3) participate and learn diverse methods of data entry and statistical analysis and report results in peer-reviewed scientific journals; 4) have good communication skills in both oral and written English; and 5) work efficiently and effectively under minimum supervision. The student will participate in a research program that consists of multiple-disciplinary scientists that closely collaborate with research institutes and universities. The proposed work would allow the potential student to get acquainted with research activities at a Canadian National Research Laboratory. The student will be trained how to establish a database and work with them statistically, how to write and publish scientific papers in English peer-reviewed journals and present the data to colleagues at scientific conferences. (Update: January 28, 2009) Page 23 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Lethbridge_05 Back PROJECT TITLE / TITRE DU PROJET: Hydrogen energy production through bio-fermentation of livestock manure Internship location in Canada/ Lethbridge Research Centre, Internship Duration/Durée du Emplacement du stage Lethbridge Alberta stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: haoxy@agr.gc.ca d’AAC) Xiying Hao Phone/Téléphone: 1-403-317-2279 Name of Research Centre/Nom du Centre de Recherche : Lethbridge Research Centre Mailing address/Adresse postale du Centre : 5403 1st ave S, Lethbridge, Alberta, Canada T1J 4B1 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Xiying Hao, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Chi Chang and Tim McAllister University collaborators/Collaborateurs universitaires: Dr. Angus Chu, University of Calgary Industry partners/Partenaires industriels: Canadian Cattlemen Association B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: With the increase in Canadian intensive livestock production, animal manure management is often problematic and has been associated with environmental problems such as water and air pollution, resulting in negative public reaction. If animal manure could be used for bio-hydrogen production, this would not only contribute to better animal manure management practices but also produce a clean fuel that reduces greenhouse gas emissions. Traditionally, animal manure is used to produce methane through fermentation. However, unlike methane, hydrogen used as a fuel source will produce only H 2O, not CO2. Therefore, there are advantages in producing hydrogen rather than methane from animal manure. More research is needed to investigate the anaerobic fermentation conditions for different waste substrates and combinations, and to analyze the efficiency of mixed waste sources for hydrogen production. By understanding how the process works for various types of wastes and waste mixtures, schemes for the best utilization of these different organic wastes will be developed. This will also provide data that is fundamental to the engineering of any fullscale facility designed to bioprocess waste for the generation of hydrogen, thereby contributing to Canadian bio-based energy systems and technologies and reducing our reliance on fossil fuel. Objective/Objectifs: This research investigates optimal conditions to produce hydrogen gas using livestock manure and other organic waste. The objectives of this project are to: 1) explore the factors affecting hydrogen production and optimize fermentation conditions and feedstock composition for hydrogen production 2) explore the feasibility of applying 2-phase anaerobic digestion to hydrogen and methane production 3) genetically “finger print” the microbial community responsible for hydrogen production and explore potential use of bacteria to increase hydrogen production Cattle feedlot manure, liquid potato processing waste, and meat processing by-products (specified risk materials: SRM) will be tested as substrates in this H2 fermentation study. Outcome expected/Résultats attendus: 1) Recommendations for optimal conditions for hydrogen production using livestock manure and other organic waste. 2) 1 to 2 scientific manuscripts addressing hydrogen production through bio-fermentation of livestock manure. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. (Update: January 28, 2009) Page 24 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Program: The cattle feedlot manure will be incubated alone and in co-incubations in a continuously-stirred batch process at various temperatures. Gas samples will be collected daily and the concentrations of H 2 and other gaseous products (CO2, N2, CH4) will be determined. Slurry samples will also be taken throughout the fermentation process and analyzed to determine total organic nitrogen, total organic carbon, available nitrogen, total carbohydrates, volatile suspended solids and microbial community composition. Experiments will be conducted to investigate (1) the effect of substrate:inoculum ratios used for co-fermentation of various organic wastes with cattle manure on hydrogen production, (2) the effect of temperature shifts on hydrogen production, and (3) the feasibility of applying 2-phase anaerobic digestion to hydrogen and methane production. Anaerobic digestion will be divided into two stages; the first will emphasize hydrogen production (as in Experiment 1), while the second will utilize the effluent from the first stage as a substrate for methane production. By using two digestion stages, usable bio-energy in the form of hydrogen and methane is being produced, while increasing the stability of the effluent generated. This will increase the total energy yield obtained while reducing the potential environmental impact of effluent from the system. Microbial analysis focusing on ‘genetic fingerprints’ of the overall community composition will be performed utilizing denaturing gradient gel electrophoresis techniques targeting PCR-amplified 16S rRNA gene fragments. This will allow comparisons between trials, aiding in the interpretation of the results obtained from chemical and physical analyses. Qualification: Ph.D. specializing in soil science, agronomy, biology or environmental chemistry. Benefit to student: The new PDF will interact and work side-by-side with other researchers in this group and gain first-hand knowledge on how to conduct agronomical and environmental research. This includes how to design and plan the logistics to conduct rigorous scientific experiments. The PDF will learn how to prepare, extract and digest livestock manure and other organic waste samples and techniques to analyze these samples using an auto-analyzer, atomic absorption machine, and ion chromatograph and analyze gas samples on gas chromatography (GS). The PDF will learn proper data management and record keeping and how to conduct statistical analysis. Finally, the PDF will learn how to write scientific papers in English for publication. Being able to work in an English environment and write papers in English is important to future international collaboration and ensures that results are disseminated to the widest possible audience. For the Lethbridge Research Centre, the opportunity to bring in top potential researchers from all over world helps us build ongoing collaborations and international networks. These PDFs could be our research collaborators for many years to come. (Update: January 28, 2009) Page 25 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_01 Back PROJECT TITLE / TITRE DU PROJET : Management of postharvest diseases in tree fruits Internship location in Canada Southern Crop Protection and Food Research Centre Internship Duration/Durée du Emplacement du stage Vineland Station, Ontario stage: 19 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: errampallid@agr.gc.ca d’AAC) Deena Errampalli Phone/Téléphone: 1-905-562-4113 ext. 234 Name of Research Centre/Nom du Centre de Recherche : AAFC-Southern Crop Protection Food Research Centre, Mailing address/Adresse postale du Centre : 4902 Victoria Ave. N., Vineland Station, Ontario, L0R 2E0, Canada Website/site Web: http://www4.agr.gc.ca/AAFC-AAC/display-afficher.do?id=1180640801098&lang=e A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Deena Errampalli, Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Peter Sholberg, AAFC, Summerland, BC University collaborators/Collaborateurs universitaires: Dr. Jennifer DeEll, OMAFRA/University of Guelph Industry partners/Partenaires industriels: Grape and Tenderfuit Ontario Ltd. B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Field based horticultural crop production represents a significant component of agriculture in Canada. To enable the year long supply of the fresh produce, producers from the southern hemisphere countries were able to directly export their produce to the northern hemisphere countries and vice versa. Long distance marketing for the horticultural crops was made possible by improved technologies in extending storage life of the commodity, storage facilities and faster and better transport systems. One such development in the recent years is the use of 1-methylcyclopropene (1-MCP) to preserve the quality of fresh produce. 1-MCP is an ethylene inhibitor. Ethylene is the precursor for disease resistance genes in plants. Dr. Errampalli’s research has shown that the effect of 1-MCP depends on apple cultivars (Errampalli et al. 2007). One of the issues to be addressed is the effect of 1-MCP on pears and peaches. The second issue that affects the fruit quality is the postharvest diseases. Significant crop losses attributed to storage rots have been reported worldwide. The control of postharvest diseases of apples and pears stored commercially depends on fungicides applied as postharvest dips or drenches. Thiabendazole (TBZ, Mertect) was the only registered postharvest fungicide for apples (until 2005) and the extensive use of this fungicide over two decades has resulted in the development of TBZ-resistant strains of storage rot pathogens (Errampalli and Brubacher 2006). One of the fungicide resistance management strategies is to alternate or combine fungicides with different modes of action. In recent years biological control agents, and new chemistries that have different mode of action than the TBZ are being developed. Objective/Objectifs: To determine the effect of 1-MCP on the management of postharvest diseases of tree fruits (apples, pears and peaches) with a biological control agent and two reduced risk fungicides. Outcome expected/Résultats attendus: 1) the effect of 1-MCP on diseases of apples, pears and peaches will be determined and 2) the efficacy of the control agents against postharvest diseases of tree fruits in the presence of 1-MCP will be assessed. References: Errampalli. D., and Brubacher, N.R. 2006. Biological and integrated control of blue mold (Penicillium expansum) of apples by Pseudomonas syringae and cyprodinil Biological Control 36:49-56. Errampalli, D., DeEll, J. and Murr, D. 2005. Effect of 1-Methylcyclopropene treatment on postharvest decay in apple cvs. Empire and McIntosh. Canadian Journal of Plant Pathology 27:467. (Update: January 28, 2009) Page 26 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship Program: 19 months: August & September 2009 –May 2010: A) Peach, pear and apple fruit will be harvested at the optimum commercial maturity required for long-term storage, and treatment with 1-MCP. Fruit will be treated with reduced-risk fungicides and biological agent and stored for 8 months. All of the pears and apples will be evaluated for disease incidence at monthly intervals (Lab and cold storage). June-July 2010: C) Data analysis from above experiments August 2009-December 2010 D) Detection and identification of the pathogens with traditional and molecular techniques & data analysis. E) Preparation of a research publication to be submitted to International scientific journal; will present results at a regional conference. Expected student qualifications : Ph.D. in Plant Pathology or Plant Sciences Benefits to the student: The student will have an opportunity to work on a multi-disciplinary project (plant pathology and plant physiology) and learn traditional and molecular techniques in the detection of the pathogens and identification of diseases of tree fruits; 2) will prepare a report and have an opportunity to make research presentation at a regional conference and finally, 3). will develop skills of collaboration and team work in a research laboratory in Canada. (Update: January 28, 2009) Page 27 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_02 Back PROJECT TITLE / TITRE DU PROJET: Developing Novel Genetic Resistance to Plum Pox Virus Internship location in Canada/ Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, Ontario stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: wanga@agr.gc.ca d’AAC) Aiming Wang Phone/Téléphone: 1-519-457-1470 ext. 313 Name of Research Centre/Nom du Centre de Recherche: AAFC-Southern Crop Protection and Food Research Centre Mailing address/Adresse postale du Centre: 1391 Sandford ST, London, Ontario N5V 4T3, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Aiming Wang, Genomics and Biotechnology Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: University of Western Ontario Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Plum pox virus (PPV) is the causal agent of the most devastating viral disease on many stone-fruit species (Wang et al. 2006). It has been found in many fruit-producing countries. Currently there is no effective way to control the virus. No natural resistance can be introduced into conventional breeding programs. The development of genetic resistance to PPV through biotechnology becomes an attractive approach to the control of the virus. Like other plant viruses, PPV has a small genome and thus must rely on host gene products (host factors) for translation, replication and infection. Mutation or silencing of these host factors, yet dispensable for plant biology, will generate novel recessive resistance to PPV. Identification of host factors is critical for the development of novel strategies against PPV. Objective/Objectifs: Recently using a number of genomics tools such as EST, cDNA-AFLP and microarray, the Wang lab has identified a few hundred of candidate genes for host factors (Wang et al. 2005; Babu et al. 2008). The objective of this research is to screen for recessive resistance genes from these candidate genes and further characterize these genes using Arabidopsis mutant lines. Outcome expected/Résultats attendus: This proposed project will advance our understanding of molecular PPV-host interactions and identify target genes for the development of novel genetic resistance against PPV. Agriculture/horticulture development will directly benefit from this research. The findings resulting from this research will be presented in academic conferences and submitted to peer-reviewed journals for publication. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. This postdoctoral internship program will provide a postdoctoral scientist to work in this genomics and molecular virology laboratory. Aiming at academic excellence, the hosting lab resides in a research centre equipped with state-of-the-art facility for research in the areas of plant molecular biology, genomics, biotechnology and biochemistry. The hosting lab has extensive experience in training technicians, postdoctoral scientists and graduate students with multicultural background. . The postdoctoral scientist must be fluent in English and have basic knowledge and lab experience in plant molecular biology or virology or microbiology. Under Dr. Wang’s direct supervision, the postdoctoral scientist will team up with an NSERC Visiting Fellow and a molecular biology technician to conduct the project. Thus the postdoctoral scientist will receive extensive training in the area of plant molecular biology/virology and biotechnology. (Update: January 28, 2009) Page 28 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_03 Back PROJECT TITLE / TITRE DU PROJET : Functional genomic analysis of seed storage protein deficiency in common bean (Phaseolus vulgaris): regulation of sulphur amino acid content. Internship location in Canada/ Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, ON stage: 24 months/mois Contact: Frédéric Marsolais Email/Courriel: marsolaisf@agr.gc.ca Phone/Téléphone: 1-519-457-1470 ext. 311 Name of Research Centre/Nom du Centre de Recherche : AAFC-Southern Crop Protection and Food Research Centre Mailing address/Adresse postale du Centre : 1391 Sandford St., London, ON, N5V 4T3, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Frédéric Marsolais, Genomics and Biotechnology Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Sangeeta Dhaubhadel University collaborators/Collaborateurs universitaires: Raju Datla, Gopalan Selvaraj, NRC-PBI, Saskatoon Industry partners/Partenaires industriels: Saskatchewan Pulse Growers B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: In the past 15 years, Canadian acreage of pulses has grown by 400%, because of their high value as export commodity, and significant environmental benefit due to reduced need for nitrogen fertilizer. Among pulses, common bean (Phaseolus vulgaris) is dominant for human consumption worldwide, and an important source of vegetable protein. Canada is the world’s second exporter of common bean. Despite their importance, common bean and other pulses lack the genomic resources available for other major crops. This proposal will contribute to address this gap, while focusing on an important seed quality trait. The contents of sulphur amino acids in seeds of common bean and other grain legumes are suboptimal for nutrition. A distinctive feature of Phaseolus and several Vigna species is the accumulation of high levels of a non-protein sulphur amino acid, S-methyl-cysteine, in part as a γ-glutamyl dipeptide. S-Methyl-cysteine cannot substitute for methionine or cysteine in the diet. In common bean, a deficiency in the major seed storage proteins, phaseolin, phytohemagglutinin and arcelin, results in a significant increase in sulphur amino acid content, particularly cysteine (ca. 2-fold), mostly at the expense of S-methyl-cysteine (Taylor et al. 2008 J. Agric. Food Chem. 56, 5647-5654). This increase brings the methionine and cystine content to levels slightly above FAO guidelines on amino acid requirements for human nutrition. Objectives: The objectives of this proposal are to achieve a detailed understanding of the S-methyl-cysteine metabolic pathway, and the regulation of sulphur amino acid levels in developing seeds of common bean. To attain this objective, we will develop a core platform for transcript profiling in pulse seeds, a 90K CombiMatrix microarray. Using this microarray, transcripts will be profiled in developing seeds of two genetically related lines differing in seed storage protein composition, and contrasted in S-methyl-cysteine and sulphur amino acid content. Five stages will be profiled, selected according to the developmental profile of S-methyl-cysteine accumulation. Candidate genes will be validated and further characterized. Outcome expected: The results of these studies will identify key biosynthetic genes controlling S-methyl-cysteine accumulation, based on differential transcript accumulation among the two genetic lines. This research will provide insight into how protein demand for sulphur regulates the levels of sulphur amino acids. The identity of associated metabolite signals will be investigated. The microarray results will constitute a core resource to investigate other important seed traits and related biochemical pathways, including raffinose oligosaccharides, vitamins and minerals, resistant starch and protein composition. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’ineterne. The postdoctoral intern will participate in functional genomic research, including transcript profiling using a custom microarray platform, data analysis and validation by quantitative RT-PCR. The derived hypotheses will be tested using a viral induced gene silencing system, by the characterization of recombinant enzymes, and targeted biochemical analyses of metabolites. The candidate should have a strong background in plant molecular biology and biochemistry. Dr. Frédéric Marsolais’ research program focuses on the protein chemistry of pulse crops, and the metabolism of nitrogen and sulphur in developing seeds. Drs. (Update: January 28, 2009) Page 29 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Datla and Selvaraj at NRC-PBI will be responsible for the development and production of the Combimatrix array. Dr. Dhaubhadel will provide support for the transcript profiling experiments. The intern will benefit from training in a research centre having a strong track record and expertise in functional genomics of crops. The centre is located in a state-of-the-art facility, equipped with all necessary instrumentation to carry out the research project. (Update: January 28, 2009) Page 30 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_04 Back PROJECT TITLE / TITRE DU PROJET : Development of sustainable agricultural systems through an understanding of interactions between microorganisms in the rhizosphere and corn roots Internship location in Canada Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, Ontario stage: 12-24 months/mois Contact: George Lazarovits Email/Courriel: Lazarovitsg@agr.gc.ca Phone/Téléphone: 1-519-457-1470 ext. 293 Name of Research Centre/Nom du Centre de Recherche : AAFC-Southern Crop Protection and Food Research Centre Mailing address/Adresse postale du Centre : 1391 Sandford Street, London, Ontario, Canada, N5V 4T3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor: Dr. George Lazarovits, Plant Sciences and Plant Protection University collaborators: Dr. Manish Raizada, University of Guelph Industry partners: Ontario Ministry of Agriculture and Food B – Project Description: rational, objectives, outcome expected Background, Rational, Issue: We aim to reduce the cost of production of major crops of Canada, specifically corn, by reducing the need for chemical fertilizers that continue to increase in price. This will also allow Canadian producers of corn to be more economically viable as based on the Brazilian sugarcane model. We identified several root-associated microorganisms from Ontario-grown corn that enhance the nutrient flow to corn roots, directly or indirectly. This can minimize the use of extraneous fertilizer and is useable by organic growers. In greenhouse and field tests the bacteria consistently improved the growth of corn and also vegetable crops. In initial tests, the growth promoting ability of the microbes appears to be cultivar-specific but not plant specific. Current methods used to screen and measure microbial nitrogen fixation are time consuming and expensive. In this project we will screen potential beneficial endophytic microbes using polymerase chain reaction assay (PCR) to identify microbes that have potential biological activities such as N fixation, antibiotic production, phosphate solubilization, etc. The microbes selected will bet tested in model assays for growth promotion under low N and P inputs, under disease pressures form various root pathogens etc. For such information we will develop soil microbial inoculants as biofertilizers. Objective : 1) Create a collection of known and potential microorganisms from the rhizosphere of corn and potato; 2) Identify potential agents for plant growth promotion using known genes associated with such functions eg. N fixation, antibiosis etc. ; 3) Screen such agents using model systems having low nutrient inputs and high disease pressures; 4) Develop some sense of mode of action for the microbes using gnotobiotc assays where plants are grown in various substrates under limited nitrogen conditions; 5) Create consortia that may enhance plant growth; 6) Mark microbes with tags for rapid identification on/in root systems Outcomes expected: This project addresses the critical need for low cost biomass production based on ecological principles for plant cultivation. We expect to identify an array of biological agents that enhance the fertility of various biofuel and food crops, developed novel screening tools as to how to identify and quantify organisms in the rhizosphere, develop methods to bring them to commercial use, and implement agricultural systems to maintain their populations in soil. We expect to enhance the science of soil microbiology and the economic health of the biofuels and food production industry by delivering highly effective biofertilizers for diverse crops. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne The intern will participate in all aspects of our laboratories’ activity. Their specific tasks will be to isolate microbial agents from the root zone and screen them using gene specific probes and PCR for specific genes considered beneficial to plant growth. Using plant growth as an indicator they will screen these bacteria for growth promotion by exposing plants directly to such agents and then growing them under various stress conditions. Consortia and individual microorganisms will then be field tested for yield benefits. The candidate should have experience in microbiology, sterile technique and handling of plants for growth assays. Some experience in molecular biology would be useful but is not a necessity. There will be opportunities to interact with two postdoctoral fellows, graduate and udnergradute students. (Update: January 28, 2009) Page 31 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_05 Back PROJECT TITLE / TITRE DU PROJET : Plant pathogenic Phytophthora: molecular determinants of virulence Internship location in Canada/ Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, Ontario stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: gijzenm@agr.gc.ca d’AAC) Mark Gijzen Phone/Téléphone: 1-519-457-1470 ext 280 Name of Research Centre/Nom du Centre de Recherche : AAFC-Southern Crop Protection and Food Research Centre Mailing address/Adresse postale du Centre : 1391 Sandford Street, London, ON, Canada N5V 4T3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Mark Gijzen, Genomics and Biotechnology University collaborators/Collaborateurs universitaires: University of Western Ontario Industry partners/Partenaires industriels: Ontario Soybean Growers, Soy20/20 B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: Plant pathogens destroy crops and impede production of food, fiber, and biofuels. Pathogens may also be invasive organisms that damage natural ecosystems, parkland, and urban landscapes. Overall, plant pathogens threaten our security and quality of life. Proper control of plant pathogens relies on a multitude of monitoring, diagnostic, and eradication techniques. Safer and more effective control strategies for many plant pathogens are required, since these organisms are often overlooked and understudied. This research is aimed at developing better management strategies for plant diseases caused by oomycetes, especially species from the genus Phytophthora. Objective/Objectifs: This research is on a disease problem that affects all soybean growing areas. The disease is caused by a soil borne pathogen Phytophthora sojae. It produces a root and stalk rot of soybean that reduces the yield and quality of the crop. The objective of the research is to discover the molecular and genetic factors that mediate this host-pathogen interaction, for the purpose of developing better diagnostic and control measures. Soybean losses due to Phytophthora root rot impact production wherever soybeans are grown. Many other crops, ornamental plants, and even natural environments are damaged by Phytophthora species, since these organisms are all destructive and invasive plant pathogens. The study of the interaction between P. sojae and soybean additionally provides a model for other Phytophthora diseases. Outcome expected/Résultats attendus: The expected outcome for the Postdoctoral candidate will be co-authorship on a publication derived from the research project. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The Postdoctoral candidate will be engaged in functional genomics work, using genome sequence information from the pathogen (Phytophthora sojae) and from the host (Glycine max) to find targets for functional characterization. The genome sequences of P. sojae and soybean (G. max) are now available and offer new opportunities for discovery. The candidate will use bio-informatics to mine sequence and gene expression databases to select candidate genes that are likely to be important determinants in the P. sojae - soybean interaction. For example, P. sojae genes that determine race-cultivar compatibility are known as avirulence (Avr) genes. It is necessary to identify all the P. sojae Avr genes because this will greatly aid breeding and diagnostics. Other genes that are crucial for pathogen growth in the host, and for soybean resistance to P. sojae, also need to be identified. Candidate genes will be systematically chosen form genome sequence data and compared among different strains of the pathogen or host cultivars. Bioassays will be preformed to measure the effect on the virulence and aggressiveness of the pathogen, or level of resistance of the host. The work may also involve genetic mapping using molecular markers, and expression of proteins in E. coli for purification and characterization. The candidate will benefit from working in a modern biochemical laboratory engaged in molecular genetic and genomic research. The organisms under study are economically important. The research is practically driven. The candidate will learn how to conduct experiments, assembly the results, draft a manuscript, and carry this through to publication in a major international journal. (Update: January 28, 2009) Page 32 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_06 Back PROJECT TITLE / TITRE DU PROJET: Developing reduced-risk and biological-based strategies for management of soilborne diseases of vegetable crops and promotion of plant growth Internship location in Canada/ Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, Ontario stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: Pervaiz.Abbasi@agr.gc.ca d’AAC) Pervaiz A. Abbasi Phone/Téléphone: 1-519-457-1470 ext. 696 Name of Research Centre/Nom du Centre de Recherche : AAFC-Southern Crop Protection & Food Research Centre Mailing address/Adresse postale du Centre : 1391 Sandford Street, London, ON, Canada N5V 4T3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Pervaiz A. Abbasi, Plant Sciences and Plant Protection Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Production of vegetables is vital to the economic health of a country. Plant diseases caused by various bacterial and fungal pathogens are one of the key limiting factors in vegetable production with estimated annual losses of 8-23% or more in terms of the yield and quality of the produce. Organic amendments animal by-products, poultry by-products, fish by-products, plantbased products, manures, and composts play an important role in organic production systems both as nutrient source and disease management tools. It is now a well known fact that soil-borne plant diseases are less severe in organically-managed soils than in conventionally-managed soils. Organic amendments can also provide an ideal matrix for delivery of bio-control agents and can enhance the performance of a microbial product in the field. The main constraint in using organic amendments for control of soil-borne diseases is the inconsistent field efficacy results and high rates of applications. Our focus is to minimize this field inconsistency, enhance disease control effects, and reduce high application rates of organic amendments by formulating them with known or other biological control agents. The organic amendments we are interested in include fish emulsion, corn distillery products, composts, and any other products which are readily available in large quantities. Once suitable combination of biological control agents and organic materials are established through growth room bioassays with target soil-borne pathogens, efficacy of the formulated products can be tested under micro-plot or field conditions. We also anticipate to tag our key bio-control agent with antibiotic resistance and GFP markers for monitoring its populations in the soil and plant and to study its interactions with pathogens in the presence of organic materials. Objective/Objectifs: Evaluate reduced-risk and biological-based strategies for managing damping-off, root rots, and wilts of vegetable crops and enhance disease control effect of organic amendments with bio-control agents. Outcome expected/Résultats attendus: The ultimate aim is to reduce pesticide use and minimize health impacts on humans and environment, and to provide control measures for soilborne diseases in the organic production systems. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The applicant is expected to possess a Ph. D degree in Plant Pathology or any related discipline in Biological Sciences. The internship will involve: developing or modifying or setting up plant bioassays, perform molecular biology techniques, isolate and screen potential new biological control agents, and formulation work. The applicant will learn new techniques and the internship will increase his/her knowledge and understanding. (Update: January 28, 2009) Page 33 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_London_07 Back PROJECT TITLE / TITRE DU PROJET : Characterizing plant-produced human interleukin-24 Internship location in Canada/ Southern Crop Protection and Food Research Centre, Internship Duration/Durée du Emplacement du stage London, Ontario stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: menassar@agr.gc.ca d’AAC) Rima Menassa Phone/Téléphone: 1-519-457-1470 ext 270 Name of Research Centre/Nom du Centre de Recherche : AAFC- Southern Crop Protection and Food Research Centre Mailing address/Adresse postale du Centre : 1391 Sandford St, London, ON, Canada N5V 4T3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Rima Menassa, Genomics and Biotechnology Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: Plantigen Inc. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: The human interleukin-24 gene is being developed for the treatment of various cancers in humans, as it targets and causes apoptosis specifically in cancer cells but not in normal cells. The gene has been shown to successfully reduce the size of tumors when applied as gene therapy in an adenoviral vector. However, this delivery method does not provide enough IL-24 to the tumors, and it is thought that applying enough recombinant protein directly to the tumors might achieve a stronger effect. We have produced the human interleukin-24 protein in plants and plant cell lines. We would like now to characterize this protein by determining its glycosylation patterns and biological activity. This characterization will allow us to define the possible applications in cancer treatment of this recombinant protein. Objective/Objectifs: To determine expression levels of the recombinant protein in the plant cell lines To purify the recombinant IL-24 from high expressing plant cell lines To determine the glycosylation levels and patterns of plant-produced IL-24 To determine the biological activity of plant-produced IL-24 in cancer cell lines Outcome expected/Résultats attendus: One or two publications are expected to be published from the results obtained. We already have filed a patent application for the production of IL-24 in plants. Further, a major outcome of this project will allow us to determine if plant-produced IL-24 can be used for the treatment of cancer C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The fellow will work within a broad program in molecular biology and biotechnology at the Agriculture Canada Research Station in London, Ontario, which houses state of the art facilities for molecular biology research. The fellow will work independently within a multidisciplinary team composed of plant scientists, immunologists and medical doctors to develop the potential use of interleukin-24 as a cancer therapy. This position requires a strong background in protein expression and purification, basic knowledge in immunology and cell culture, and an excellent mastery of the English language. This position will allow the fellow to develop their scientific potential, and should lead to the publication of at least one scientific article. (Update: January 28, 2009) Page 34 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Ottawa_01 Back PROJECT TITLE / TITRE DU PROJET : Biological management of fusarium head blight in wheat Internship location in Canada/ Eastern Cereal and Oilseed Research Centre, Internship Duration/Durée du Emplacement du stage: Ottawa, Onatario stage: 12 -24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: axue@agr.gc.ca d’AAC) Allen Xue Phone/Téléphone: 1-613-759-1513 Name of Research Centre/Nom du Centre de Recherche : AAFC-Eastern Cereal and Oilseed Research Centre (ECORC) Mailing address/Adresse postale du Centre : KW Neatby Building, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Allen Xue, Research Scientist, Plant Pathology Other AAFC collaborators/Autre collaborateurs à AAC: Drs. M. Savard, H. Voldeng, G. Fedak University collaborators/Collaborateurs universitaires: Dr. Tom Hsiang, University of Guelph Industry partners/Partenaires industriels: Ontario Wheat Producers Marketing Board B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Wheat is the largest crop in both Canada, and fusarium head blight (FHB) has been a devastating disease that causes extensive yield and quality losses to wheat in both countries. Wheat cultivars with high levels of resistance to FHB are not yet commercially available, and foliar application of fungicides has been the only mean to combat the disease in both countries. However, the fungicide treatment is not desirable due to some adverse effects on the environment and ecosystem, and an improved FHB management system with reduced fungicide use is urgently needed. Since 2005, Dr. Xue at ECORC has led studies in identifying a more natural and environmentally acceptable alternative to the existing chemical treatment methods. His research demonstrated that a strain of Clonostachys rosea, ACM941, and a strain of Trichoderma harzianum, Tr14, and a strain of Bacillus subtilis ACO071 are effective bioagents against FHB pathogens in vitro and in greenhouse and field conditions. A further in-depth research is underway to examine the mechanisms of these bioagents against FHB pathogens, the population dynamics in various wheat production systems and their impact on reduction of initial inoculum of FHB after foliar application, and the compatibility of bioagents with registered fungicides for potentially enhanced effectiveness for the control of FHB. Research on the development of experimental commercial formulations are underway in collaboration with Cornell University to make new biocontrol products more stable, effective, safe, and cost-effective. Objective/Objectifs: The objectives of this project are to evaluate selected microbe bioagents and formulated products of these bioagents for their efficacy in reducing the initial inoculum by suppressing the perithecium production in various wheat production systems and in reducing FHB disease severity, Fusarium damaged kernels, and Mycotoxin levels in kernels by foliar application, in comparison with Folicur (tebuconazole) fungicide. Outcome expected/Résultats attendus: The successful completion of this project will lead to the development of commercial formulations of the bioagents. The new technologies will be transferred to the grain industries through licences and technology transfer agreements to interested companies. Results will be published in at least two peer-reviewed articles in scientific journals. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The PDF will work with members of the Crop Pathology Team at ECORC conducting majority of the research experiments to evaluate and validate the efficacy of selected bioagents in controlling FHB. Depending on the PDF’s experience, training may be provided in data collection, statistical analysis, and preparation of research reports and manuscripts. The PDF will also have the opportunity to interact with a number of excellent scientists in cereal breeding, cytogenetics, mycotoxin, and molecular biology at ECORC and University of Guelph. Candidates with some background training and experience in biological control of plant diseases would be desirable. (Update: January 28, 2009) Page 35 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Ottawa_02 Back PROJECT TITLE / TITRE DU PROJET : Phytochemical Resistance Mechanisms to Corn Pests Internship location in Canada/ Eastern Cereal and Oilseed Research Centre, Internship Duration/Durée du Emplacement du stage Ottawa, Onatario stage: 12 months/mois Contact: Lana M. Reid Email/Courriel: reidl@agr.gc.ca Phone/Téléphone: 1-613-759-1619 Name of Research Centre/Nom du Centre de Recherche : AAFC-Eastern Cereal and Oilseed Research Centre (ECORC) Mailing address/Adresse postale du Centre : Bldg. 99-Central Experimental Farm, 960 Carling Ave., Ottawa, ON, Canada K1A 0C6 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Lana M. Reid, Research Scientist, Corn Breeding Other AAFC collaborators/Autre collaborateurs à AAC: Xiaoyang Zhu, Biologist, Corn Pathology University collaborators/Collaborateurs universitaires: Dr. John T. Arnason, University of Ottawa Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: AAFC has developed many corn varieties with improved resistance to eight of the major diseases of corn found in Canada. These resistant varieties were developed with conventional breeding protocols. In some cases, it is not clear what the underlying mechanism of resistance to a given disease is. Understanding these mechanisms will lead to the development of rapid screening tools to assist in the development of higher levels of resistance and a greater understanding of the genetics of this resistance. Dr. Reid and Dr. Arnason have a long standing collaborative research program to investigate phytochemical resistance mechanism in corn. Dr. Reid, as a corn breeder, and Mr, Zhu, as a corn pathologist, breed and identify the resistant germplasm; Dr. Arnason, as a phytochemist, conducts the laboratory analysis of the potential phytochemical resistance factors. Objective/Objectifs: To further our investigations into the role of phytochemicals in resistance to corn diseases, with an emphasis on di-ferulates and resistance to Fusarium species. Outcome expected/Résultats attendus: Increased co-operation to further the development of corn varieties resistant to major economically important diseases in both countries. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. In the summer and fall, the student will work primarily in the experimental plots of AAFC with Dr. Reid to learn corn breeding and specifically, breeding for pest resistance. The PDF/student will learn how to inoculate plants with diseases, assess resistance and collect plant tissue samples. In the winter months, the student will work at the University of Ottawa with Dr. Arnason to conduct a phytochemical analysis of the samples he/she collected in the field. The intern will return to the home country with a greater understanding of North American corn breeding and phytochemisty; the intern will learn how to integrate this new knowledge into improving corn or other crop production in the home country. Candidates should have some basic understanding of plant genetics and breeding and/or chemistry. (Update: January 28, 2009) Page 36 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Ottawa_03 Back PROJECT TITLE / TITRE DU PROJET: Management and total use of agricultural residues for biofuels, bioproducts and bioenergy Internship location in Canada/ Eastern Cereal and Oilseed Research Centre, Internship Duration/Durée du Emplacement du stage Ottawa, Onatario stage: 12-24 months/mois Contact: Carlos Monreal Email/Courriel: monrealc@agr.gc.ca Phone/Téléphone: 1-613-759-1053 Name of Research Centre/Nom du Centre de Recherche : AAFC-Eastern Cereal and Oilseed Research Centre (ECORC) Mailing address/Adresse postale du Centre : KW Neatby Building, 960 Carling Ave., Ottawa, ON, Canada K1A 0C6 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Carlos Monreal, Research Scientist, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Drs. M. Schnitzer, R. Lemke, T. Huffman, E. Pattey, R. Desjardins University collaborators/Collaborateurs universitaires: University of Ottawa and Carleton University in Canada, and other foreign universities. Industry partners/Partenaires industriels: Advanced Biorefineries Inc., Highmark Renewables, Enerkem Inc. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Agricultural residues, especially animal manures and crop residues, have no economic value in spite of their high nutrient and energy content. The pathogens existing in animal residues create health related problems to humans and wildlife. Agricultural residues may also contribute to environmental contamination with excess nutrients and GHG emissions. The disposal of the latter residues in municipal landfills increases the cost of their disposal and the cost of food production to farmers and the agrifood industry and translates into higher food prices for consumers. In comparison, the use, conversion and processing of agricultural residues alone or in combination with municipal, food, of forest residues into biofuels and bioproducts create new economic opportunities in Rual communities by creating new value-added bioproducts in biorefineries. Objective/Objectifs: a) to establish benefitial management practices for the handling of animal manures in the intern’s home country; b) create value-added for agricultural residues through the production of bioenergy, biofuels and bioproducts derived from the conversion of agricultural and other biomass; b) enhance knowledge on the carbon and nitrogen cycles associated with agriculture in the northern and southern hemispheres; c) support the commercialization of leading-edge Canadian technologies for the conversion of agricultural residues; and c) to enhance the environmental, economic and social performance of agriculture. Outcome expected/Résultats attendus: production of: i) second generation biofuels (i.e., biooil and biogas), ii) bioenergy (electrical and thermal), and iii) bioproducts (i.e., polyesters for bioplastics, biofertilizers); ii) scientific R&D networks for enhancing knowledge on the carbon and nitrogen cycles associated with cultivated land and food production systems; iii) scientific and technical networks for the utilization of residues, and the reduction and monitoring of net greenhouse gas emissions; iv) development and commercialization of Canadian technologies and bioprocesses. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The Post-doctoral fellow(s) will spend time in the lab of Dr. Monreal and others at AAFC and universities enhancing knowledge of complex living and chemical systems, learning new techniques of nano- and bio-technology and developing new knowledge and bioprocesses that will contribute to better manage carbon and nitrogen during food production and the successful establishment of biorefineries in rural communities. Arrangements will be made with industry for the Post-docs to spend time with Canadian industry and farmers for getting acquainted with leading-edge primary biomass conversion technologies of pyrolysis, anaerobic digestion and gasification useful to farm operations and rural communities. (Update: January 28, 2009) Page 37 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Ottawa_04 Back PROJECT TITLE / TITRE DU PROJET: Adapting micrometeorological techniques for verifying GHG emissions from agricultural fields and paddocks to desired conditions Internship location in Canada/ Eastern Cereal and Oilseed Research Centre, Internship Duration/Durée du Emplacement du stage Ottawa, Onatario stage: 24 months/mois Contact: Elizabeth Pattey Email/Courriel: elizabeth.pattey@agr.gc.ca Phone/Téléphone: 1-613-759-1523 Name of Research Centre/Nom du Centre de Recherche : AAFC-Eastern Cereal and Oilseed Research Centre (ECORC) Mailing address/Adresse postale du Centre : KW Neatby Building, 960 Carling Ave., Ottawa, ON, Canada K1A 0C6 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Elizabeth Pattey, Research Scientist, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Ray Desjardins, Ward Smith, Dave Dow, Stuart Admiral University collaborators/Collaborateurs universitaires: Dr. Ian Strachan Industry partners/Partenaires industriels B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: National greenhouse gas (GHG) inventory calculation are developed using IPCC methodology. Countries are encouraged to develop their own sets of emission factors which better accounts for the influence of environmental conditions and management practices. This is referred as Tier 2 methodology in IPCC. The country-specific methodology can be further refined by using process-based modelling in mass balance approach, which corresponds to a Tier 3 methodology. Using a country-specific methodology is particularly important for calculating the nitrous oxide emissions from the agricultural sector. Indeed, the complexity of biological and physical controls on microbial processes that generate N 2O in soils and animal wastes has caused huge uncertainty in estimating N2O emissions from agricultural sources. More site-specific N2O emission factors are needed for GHG inventories that account for variability caused by climate, soil and manure management, and topography. Moreover, these site-specific datasets can be used to verify process-based models in preparation of Tier 3 approach. Any new emission factors should be scientifically documented and peer-reviewed to be accepted in IPCC. Nitrous oxide emissions are well-known to be highly variable over space and time. These emissions are driven by both nitrification and denitrification processes. The latter takes place under anaerobic conditions and usually leads to higher N 2O production. In order to capture the spatial and temporal variability at the scale of the production unit, (e.g., field, paddock, manure storage facility), micrometeorological techniques need to be adapted to these appropriate scales and conditions. They can provide N2O emissions on a continuous basis and with lower uncertainty than using a chamber approach alone, which usually integrate emissions over a square meter or less, thus requiring scaling-up. Our laboratory has a long experience in developing and refining micrometeorological techniques. The laboratory has four tunable diode lasers, all the software for supporting the various techniques, more than a decade of experience in measuring N 2O emissions as testified by the scientific publications on the topic. We also calculate the national GHG emisisons from the Canadian agricultural sector. Objective/Objectifs: Provide a post-doctoral internship on measuring N2O emissions from diffuse agricultural sources using several micrometeorological techniques, in order for the target country to develop specific N2O emissions factors and verify the performance of process-based models under the wide range of climate and soil environments that prevails in the target country. Outcome expected/Résultats attendus: This project will allow the training of highly qualified personnel in trace gas flux measurement using both tower and chamber approaches. This expertise will be useful to setup a trace gas flux laboratory in the target country and establish collaborative work with us. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The emphasis will be put on the micrometeorological approach and the PDF will become familiar with the operation of a (Update: January 28, 2009) Page 38 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC tunable diode laser and other instruments used to measure fluxes from diffuse sources (such as sonic anemometers and infrared gas analyzers). The intern will have the opportunity to use chambers and gas chromatograph for measuring GHG fluxes and to analyze datasets from chambers and towers. The internship will cover the various phases of the experimentation, from setting up and operating the equipment, acquiring and processing the data, analyzing the emissions with regard to the environmental conditions and comparing the N2O emissions with estimates from models. The candidate will have the opportunity to be exposed on how we compute the agro-environmantal indicator on GHG emission of Canada. The PDF should have a good background in physics or meteorology, gas laws, statistics, ecology, knowledge in agricultural practices. The PDF will join a team made of scientists, research professionals and a technician with complementary expertise, providing an opportunity to cover the various phases of the research including the submission of scientific manuscript. (Update: January 28, 2009) Page 39 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Quebec_01 Back PROJECT TITLE / TITRE DU PROJET : Identification of functional markers associated to superior freezing tolerance in alfalfa. Internship location in Canada/ Soils and Crops Research and Development Centre, Internship Duration/Durée du Emplacement du stage Québec City, Québec stage: 12 months/mois Contact: Yves Castonguay Email/Courriel: yves.castonguay@agr.gc.ca Phone/Téléphone: 1-418-210-5012 Name of Research Centre/Nom du Centre de Recherche : AAFC-Soils and Crops Research and Development Centre Mailing address/Adresse postale du Centre : 2560 HOCHELAGA BLVD., QUÉBEC, QC, CANADA, G1V 2J3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Yves Castonguay, Scientist in Environmental Physiology, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Annick Bertrand (biochemistry), Dr. Serge Laberge (molecular biology), and Dr. Réal Michaud (forage breeding) University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected/ Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Alfalfa (Medicago sativa L.) is the most extensively used forage legume with 32 million hectares cultivated worldwide and 15 million hectares in North America. Alfalfa’s attributes include genetic variability/adaptability, growth on alkaline soils and under reduced moisture, and a deep nitrogen-fixing taproot which improves soil structure and health. In addition to its large economic value as a staple used by dairy and beef livestock producers, it is a strong biomass candidate for sustainable production of ligno-cellulosic biofuel. However, cultivars currently available in Canada, suffer from recurrent winterkill as a result of insufficient tolerance to subfreezing temperatures. In recent years, our group has applied an indoor screening method to develop alfalfa populations selectively improved for their tolerance to freezing (TF populations). Several cycles of recurrent phenotypic selection have been performed in various cultivars and large increases in freezing tolerance that translated into superior field survival in the spring were documented. Further acceleration of the breeding process could be achieved by the identification of DNA markers. For that purpose, the identification of functional markers derived from polymorphic sites within genes associated to the trait will be required for the fixation of alleles in different genetic backgrounds without additional calibration. Objective/Objectifs: Using the Sequence Related Amplified Polymorphism (SRAP) technique, we have recently identified several anonymous genomic DNA polymorphisms that increase in frequency with the number of cycles of selection for improved freezing tolerance. Although the SRAP technique is a random PCR-based approach, primers are designed to preferentially target coding sequences. Using already identified SRAP polymorphisms, the candidate will perform parallel amplification with genomic DNA and cDNA templates from cold-acclimated plants in order to identify polymorphisms located within functional sequences. DNA polymorphism putatively located within coding sequences will be cloned, sequenced and analyzed for heir homology with sequences in databank. Candidate sequences will be validated for their linkage with freezing tolerance using segregating genotypes individually scored for their freezing tolerance. Outcome expected/Résultats attendus: Combination of unique genetic resources and molecular genetics techniques will allow the identification of markers that can trace functional cold tolerance alleles during introgression into germplasm of high agronomic value. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Program: The project will be conducted over a one year period and will proceed according to the following schedule : 1- First trimester: Make an inventory of SRAP polymorphisms uncovered using populations recurrently selected for superior freezing tolerance. Clone and sequence several (10-20) fragments that are polymorphic among populations recurrently selected for superior freezing tolerance and that are equally amplified with genomic DNA and cDNA templates. (Update: January 28, 2009) Page 40 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC 2- Second trimester: Search for homologies with sequences in data bank. Analyze correlation between the inheritance of functional sequences and segregation of freezing tolerance within populations. Conduct multivariate analyses (principal component and/or discriminant analyses) to identify groups of polymorphisms that are closely associated to superior freezing tolerance. 3- Third trimester: Finalize analyses, write a scientific manuscript and prepare final report. Expected qualifications: The candidate should have completed a PhD program in agricultural plant science in a recognized university. She/he should have a background in plant breeding and have a good knowledge of the field of molecular genetics. She/he should have expertise in PCR analyses, gene cloning and sequence analyses with bioinformatics software (BLAST, FASTA). She/he should be familiar with data analyses including correlation and multivariate analyses using specialized statistical software (e.g. SAS, Genstat). She/he should also have English language skills in speech and writing. Benefits to post-doctoral candidate: The project will provide an excellent opportunity for the candidate to be trained to field of molecular genetics for species with complex genome within a multidisciplinary environment involving a physiologist, a biochemist, a molecular biologist and a plant breeder. She/he will have access to the latest techniques and equipment and will work with a research team with a recognized expertise in forage breeding and physiology. The candidate will also have access to data and genetic resources (genotypes, DNA, RNA samples) that are already available to initiate the project. This project provides a good opportunity to learn techniques in molecular breeding of species with complex genomes. (Update: January 28, 2009) Page 41 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Quebec_02 Back PROJECT TITLE / TITRE DU PROJET : Develop a particulate phosphorus (PP) component for a risk indicator of water contamination by P from Canadian agricultural land Internship location in Canada/ Soils and Crops Research and Development Centre, Internship Duration/Durée du Emplacement du stage Québec City, Québec stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: eric.vanbochove@agr.gc.ca d’AAC) Eric van Bochove Phone/Téléphone: 1-418-210-5050 Name of Research Centre/Nom du Centre de Recherche : AAFC-Soils and Crops Research and Development Centre Mailing address/Adresse postale du Centre : 2560 Hochelaga Blvd, Québec, QC, G1V 2J3, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Eric van Bochove, Research Scientist in Water Quality Other AAFC collaborators/Autre collaborateurs à AAC: Georges Thériault, Jean-Thomas Denault, Marie-Line Leclerc University collaborators/Collaborateurs universitaires: Pr. Suzanne Allaire, Laval University, Quebec, QC; Pr. David Lobb, University of Manitoba, Winnipeg, MB; Pr. Brad Joern, Purdue University, Indiana, USA Industry partners/Partenaires industriels: None B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Excessive amounts of P in surface fresh water contribute to eutrophication of rivers and lakes and to Cyanobacteria blooms. The contamination of water is of greatest concern in areas where soil tests show high P levels, ability of soils to retain P is low, susceptibility to runoff is high, macropore flow is high, soil erosion is high; and where connectivity to surface water and artificial drainage are dense. Phosphorus from agricultural land is transported by surface runoff and drainage in variable proportions of dissolved P (DP) and particulate P (PP). The Government of Canada has undertaken evaluation of the performance of its agriculture and reporting on agri-environmental indicators. The indicator of risk of water contamination by phosphorus (IROWC-P) has been recently developed at the national scale. The IROWC_P algorithm aims to integrate both annual risks of water contamination by DP and PP for the agricultural land of Canada at the watershed scale. IROWC_P is a multiplicative equation between phosphorus source (P_Source) and transporthydrology (T_H) components. P_Source represents the proportion of DP that can be potentially desorbed by agricultural soils of a watershed during a Census of agriculture year. The T_H component integrates both DP and PP transport processes (surface runoff, drainage and water erosion) as well as other factors accounting for hydrological connectivity between P source and water bodies (topographic index, tile drainage, surface drainage and preferential flow). The total proportion of PP is indirectly estimated by the amount of soil sediments removed by water erosion and transported to streams as a function of P saturation expressed by P_Source. The P_Source component is a function of a 30-year cumulative P_Balance (1976 to 2006) and soil P saturation. Final IROWC_P modified values were associated to five vulnerability classes (very low, low, moderate, high and very high) on the basis of their respective distribution in 2006 to obtain a corresponding magnitude of risk for each agricultural watershed at the scale of Fundamental Drainage Areas for all Census years. Finally, the risk was calculated for 279 watersheds having more than 5% agricultural land to identify critical areas across Canada and temporal trend of P risk loss over 25 years period (from 1981 to 2006). Objective/Objectifs: Develop the methodology to better estimate the risk of PP and bioavailable PP that can be released from agricultural land of Canada by runoff events using available soil and watershed datasets at the national scale. Outcome expected/Résultats attendus: Literature review, concept and methodology integrating the new PP component to the IROWC-P indicator. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The post-doctoral fellow will be integrated in a bilingual team (French/English) of two research scientists, four research professionals, post-doctoral fellows and graduate students with access to a laboratory of water quality and soil analyses and GIS facilities working in collaboration with governmental organizations and universities across the country. (Update: January 28, 2009) Page 42 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC The candidate should have a background and/or knowledge on the phosphorus cycle and/or soil science and/or soil hydrology. Experience with risk indicators development and/or modeling will be an advantage. The post-doctoral work will be integrated within a 4-yr national project and contribute significantly to the indicator development leading to publication of peer-reviewed scientific papers in collaboration with the team of scientists partner in the project. (Update: January 28, 2009) Page 43 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Quebec_03 Back TITRE DU PROJET : Fractions protéiques et glucidiques de diverses espèces fourragères PROJECT TITLE: Protein and carbohydrate fractions in different forage species Internship location in Canada/ Soils and Crops Research and Development Centre, Internship Duration/Durée du Emplacement du stage Québec City, Québec stage: 12 months/mois Contact: Gaëtan Tremblay Email/Courriel: tremblaygf@agr.gc.ca Phone/Téléphone: 1-418-210-5048 Name of Research Centre/Nom du Centre de Recherche : AAC-Centre de recherche et de développement sur les sols et les grandes cultures / AAFC-Soils and Crops Research and Development Centre Mailing address/Adresse postale du Centre : 2560 Boulevard Hochelaga, Québec, QC, Canada G1V 2J3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Gaëtan Tremblay. Ph.D., Chercheur, Valeur nutritive des aliments du ruminant / Research Scientist, Nutritive value of feedstuffs. Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Gilles Bélanger (agronomie et physiologie des plantes fourragères / forage crop physiology and agronomy), Yves Castonguay (physiologie végétale / plant physiology), Annick Bertrand (biochimie / biochemistry), et/and Réal Michaud (génétique des plantes fourragères / forage breeding). University collaborators/Collaborateurs universitaires: Dr. Guy Allard (agronomie des plantes fourragères / forage crop agronomy), Université Laval, Québec. Industry partners/Partenaires industriels: Novalait inc. (Corporation créée conjointement par les producteurs et les transformateurs laitiers du Québec / A private corporation owned by Québec milk producers and processors). B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Contexte, Rationnel, Problématique: La dégradation intensive et rapide des protéines, combinée à une faible teneur en énergie rapidement fermentescible dans les fourrages, causent une faible utilisation de l’azote des fourrages par les ruminants, ce qui résulte en un excès d’azote excrété dans l’environnement. Background, Rational, Issue : Intensive and rapid degradation of proteins combined with insufficient readily fermentable energy in forages contributes to the poor forage N utilization by ruminants and, consequently, results in an excess of nitrogen to be excreted in the environment. Objectifs: Le projet global vise à augmenter l’énergie et l’azote protéique (fractions B2 et B3) des fourrages pour ainsi améliorer les performances du ruminant via une augmentation de la prise alimentaire et de l’efficacité d’utilisation de l’azote, causant du même coup une réduction des pertes d’azote dans l’environnement. Un des objectifs spécifiques est de caractériser les fractions protéiques et glucidiques dans les espèces fourragères cultivées dans l’Est du Canada. Objective : The overall project aims at increasing the content in energy and protein-nitrogen (fractions B2 and B3) of forages with the ultimate goal of improving the performance of ruminants through an increase in intake and nitrogen utilisation efficiency while reducing nitrogen losses to the environment. One of the specific objectives is to characterize protein and carbohydrate fractions in different forage species grown in Eastern Canada. Résultats attendus: L’estimation des fractions protéiques et glucidiques des différentes espèces fourragères, faites par spectroscopie dans le proche infrarouge, sera utilisée par l’industrie pour formuler les rations des ruminants au Canada. Outcome expected: Estimation of protein and carbohydrate fractions in different forage species, made using near infrared reflectance spectroscopy (NIRS), will be used by the industry to formulate ruminant rations in Canada. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne : Pour le stagiaire, le projet représente une excellente opportunité de faire partie d’une équipe multidisciplinaire dans le domaine de la physiologie, de l’agronomie et de la valeur nutritive des plantes fourragères. Il travaillera dans un laboratoire utilisant de l’équipement de pointe. Il utilisera les techniques de laboratoire permettant de doser les fractions protéiques et glucidiques, ainsi que la technique de prédiction de ces fractions en utilisant la spectroscopie dans le proche infrarouge (SPIR). Le projet sera réalisé entre octobre 2009 et octobre 2010 : Octobre 2009-Février 2010: Balayage par SPIR des échantillons de fourrages prélevés au cours des étés 2007 et 2008. (Update: January 28, 2009) Page 44 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Analyses en laboratoire des fractions protéiques (Cornell Net Carbohydrate and Protein System, Sniffen et al. 1992) et des fractions glucidiques (acides organiques, mono- et oligo-saccharides, amidon, et fibres solubles dans un détergent neutre, Hall et al. 1999. J. Sci. Food Agric. 79:2079) dans un certain nombre d’échantillons. Mars 2010: Calibration SPIR pour prédire les fractions protéiques et glucidiques dans tous les échantillons. Avril 2010-Mai 2010: Revue de littérature et analyse statistiques des données. Juin 2010- Septembre 2010: Rédaction d’un manuscrit scientifique. Le candidat sélectionné devra détenir un doctorat en phytologie ou en sciences animales avec expérience en laboratoire, dans l’utilisation de la spectroscopie dans le proche infrarouge, et dans l’analyse statistique des données. Il devra avoir une bonne connaissance de l’anglais parlé et écrit. La connaissance du français est un atout. Internship: Describe the internship program, qualifications of and the benefits to the expected intern The project will be an excellent opportunity for the postdoctoral student to be part of a multidisciplinary team in the area of plant physiology, agronomy, and nutritive value of forage crops. She/he will work in the laboratory using the latest equipment. The postdoctoral student will also use techniques for determination of protein and carbohydrate fractions, and for their NIRS predictions. The project is scheduled from October 2009 to October 2010 and will be organized as follows: October 2009-February 2010: NIRS scanning of forage species samples collected during the 2007 and 2008 summers. Laboratory analyses of protein fractions (Cornell Net Carbohydrate and Protein System, Sniffen et al. 1992) and carbohydrates fractions (organics acids, mono- and oligo-saccharides, starch, and neutral detergent-soluble fiber, Hall et al. 1999. J. Sci. Food Agric. 79:2079) in a subset of samples. March 2010: NIRS calibration to predict protein fractions and carbohydrates in all collected samples. April 2010-May 2010: Literature review, data and statistical analyses. June 2010- September 2010: Completion of a scientific manuscript. The selected postdoctoral student should have a Ph.D. in plant or animal science with some experience in laboratory, NIRS equation development, and in statistical data analyses. She/he should also have English language skills in speech and writing. Having French language skills would be an asset. (Update: January 28, 2009) Page 45 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_Quebec_04 Back PROJECT TITLE / TITRE DU PROJET : Evaluation of invasive weed distribution and spread into crop fields Internship location in Canada/ Centre de recherche et de développement sur les sols et les Internship Duration/Durée du Emplacement du stage grandes cultures/Soils and Crops Research and Development stage: 12 months/mois Centre, Québec, Québec Contact: Marie-Josée Simard Email/Courriel: marie-josee.simard@agr.gc.ca Phone/Téléphone: 1-418-210-5044 Name of Research Centre/Nom du Centre de Recherche : AAC-Centre de recherche et de développement sur les sols et les grandes cultures / AAFC- Soils and Crops Research and Development Centre Mailing address/Adresse postale du Centre : 2560 boul. Hochelaga, Québec, QC, G1V 2J3, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Marie-Josée Simard/Plant Ecologist-Weed Scientist Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Robert Nurse and Stephen Darbyshire University collaborators/Collaborateurs universitaires: Dr. Claude Lavoie Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte/Rationnel/Problématique: A striking number of invasive species are plants (Pimentel et al. 2005). Darbyshire (2002) estimated that 1-2 new species of weeds become established in Canada every year and that this rate is increasing. Agriculture (new crops such as biofuels and seed contaminants) is an important pathway of plant introduction into Canada (CFIA 2008). Nevertheless, the agricultural linkage (as source of new propagules and as source of landscape) of plant invasions receives limited attention from invasion ecologists that mainly study natural systems, or from weed scientists who typically investigate weed control in fields. Pastinaca sativa L. (wild parsnip) is an invasive species of increasing agronomic importance in Canada whose landscape dynamic deserves attention. Pastinaca sativa is a biennial species that has spread from Eurasia into the American continent. The species, occasionally referred to as Pastinaca sativa subspecies sylvestris, probably escaped from fields of the edible crop at the beginning of the twentieth century (Rousseau 1968; Averill and DiTommaso 2007). Wild populations of Pastinaca sativa contain furanocoumarins which cause severe skin photo-activated dermatitis in humans and livestock (Mitchell and Rook 1979) and suppress herbivory (Berenbaum 1981; Zangerl and Nitao 1998). The species invades reduced-tillage cropping systems in eastern Canada and is considered a noxious weed in Ontario (Darbyshire 2003) and several U.S. states. The presence of this poisonous weed in hay severely decreases forage quality for on-farm use and closes or restricts markets for high-value eastern Canadian hay commodities. Abundant along roadsides in eastern Canada, road networks could be contributing to the spread of the species into crops and associated agricultural landscapes (DiTommaso pers. comm.). An evaluation of the historical and current spread of the species could be used to test this hypothesis (Lavoie et al. 2005; Amor and Stevens 2008). Objective/Objectifs: Evaluate the historical distribution of Pastinaca sativa in Canada using herbarium records of the species and of other weeds. Compare the historical distribution of Pastinaca sativa with that of other weeds not found along roadsides. Evaluate the potential for spread by water along roadsides and by soil transport during road construction/refection. Outcome expected/Résultats attendus: Documenting the spread of the species in Canada will provide information on the interactions and dynamics of roadside weeds with agro-ecosystems. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship program description: The project (training) will be organized as follows: Four months of herbarium data collection and literature update (September to December 2009); Three months of herbarium data analyses (January to April 2010) and (Update: January 28, 2009) Page 46 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Four months of manuscript writing and occasional field work (participation in different phases of the field project, A-Base funding) (May to August 2010). Expected qualification: The selected applicant should have a Ph. D. in weed science, plant ecology or botany or equivalent from a recognized university. The selected intern should have a good knowledge of weed biology/dynamics, of statistical data analyses and of science. The selected intern should also have some experience in computer mapping (GIS) and in the conduct of field experiments. Benefits for the student: The project will be an excellent opportunity for the intern to be trained with a multidisciplinary team in the area of weed science, plant ecology and botany. He/She will have the opportunity to consult different national (Ottawa), university (Québec) and provincial (Québec, Montréal) herbariums, use state of the art computer programs, write a manuscript and participate in field experiment in Canada. (Update: January 28, 2009) Page 47 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Saskatoon_01 Back PROJECT TITLE / TITRE DU PROJET : Enhancing quality and function of recombinant proteins produced in seeds Internship location in Canada/ Saskatoon Research Centre, Internship Duration/Durée du Emplacement du stage Saskatoon, Saskatchewan stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: RozwadowskiK@agr.gc.ca d’AAC) Kevin Rozwadowski Phone/Téléphone: 1-306-956-7692 Name of Research Centre/Nom du Centre de Recherche : AAFC-Saskatoon Research Centre Mailing address/Adresse postale du Centre : 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Kevin Rozwadowski, Recombination Biology Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Dwayne Hegedus & Derek Lydiate University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: Seed-based synthesis of recombinant proteins is a highly attractive system for production of industrial proteins (e.g. enzymes, fibres, etc.) due to the ease of scalability, transport and storage. We have advanced technologies to promote accumulation of recombinant proteins. However, in addition to high-levels of accumulation, a fundamental requirement of seed-based expression systems is ensuring recombinant proteins retain functionality. Recently it has been demonstrated that seed proteins are extensively modified by carbonylation, an irreversible process involving the direct oxidation of some amino acid residues and their covalent linkage to lipids and reducing sugars. This process results in the inactivation of proteins and their increased proteolysis. Therefore, inhibition of the protein carbonylation process is an important consideration to ensure production of functional recombinant proteins in seed-based protein expression systems. Objective/Objectifs: The research will investigate the biology of protein carbonylation in seeds and development of methods to inhibit this process to enhance accumulation of non-carbonylated, functional recombinant proteins in seeds. The Arabidopsis model system will be used in these studies to investigate the biochemical pathways regulating oxidative state in developing seeds and evaluation of methods to suppress oxidative damage of seed proteins. This will include characterising mutant lines of Arabidopsis defective for genes involved in oxidative processes as well as creating transgenic lines expressing candidate genes expected to reduce oxidative potential in the seed. Outcome expected/Résultats attendus: The research in the Arabidopsis model system will identify strategies to reduce oxidative damage and carbonylation of proteins in seeds. These results will be applied to oilseed crucifer crop species to develop a platform for producing recombinant proteins in seeds to meet industry needs and provide an alternate revenue stream for producers. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship program—The postdoctoral intern will work in an established plant molecular biology lab conducting plant biology research in a work group consisting of experienced technicians and other post-doctoral researchers. The intern will participate in ongoing aspects of the research program and be responsible for an independent research project that is integrated with the lab’s research program. The research will involve a wide-range of molecular biology, biochemistry and genetic techniques including: gene cloning and engineering; protein engineering; protein purification and characterisation; characterising seed and whole plant development and growth; generation and characterisation of transgenic plants. Candidate qualifications—A Ph.D. in plant biology, preferably with experience in molecular biology, is required. Ability to work independently, with good oral and written communication skills in English is also necessary. Benefits to the candidate—The intern will develop comprehensive knowledge and skills in plant molecular biology transferable to a breadth of possible subsequent studies in applied and basic plant biology. (Update: January 28, 2009) Page 48 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Saskatoon_02 Back PROJECT TITLE / TITRE DU PROJET : DNA recombination and repair in plants Internship location in Canada/ Saskatoon Research Centre, Internship Duration/Durée du Emplacement du stage Saskatoon, Saskatchewan stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: RozwadowskiK@agr.gc.ca d’AAC) Kevin Rozwadowski Phone/Téléphone: 1-306-956-7692 Name of Research Centre/Nom du Centre de Recherche : AAFC-Saskatoon Research Centre Mailing address/Adresse postale du Centre : 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Kevin Rozwadowski, Recombination Biology Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: DNA recombination and repair are essential biological processes that both maintain genome fidelity in vegetative and germ-line cells and generate genetic diversity during meiosis. Double-strand breaks (DSBs) are critical, potentially lethal, DNA lesions that result from normal metabolism, environmental stress and programmed cellular processes. DSBs may be repaired by a high-fidelity, homologous recombination process or an error-prone, non-homologous end joining mechanism. The research project will focus on DSB-repair systems in plants using the model plant Arabidopsis thaliana and will build on a foundation of DNA repair research established in the Rozwadowski laboratory. The results will contribute to developing effective approaches for manipulating DNA recombination processes in crop species to enable highfrequency gene targeting. Gene targeting is a process enabling precision engineering of plant genomes via using homologous recombination to precisely alter the genetic sequence of a specified genomic target locus, as opposed to the random genetic alterations resulting from conventional transgenic approaches. In addition, the results will be applied to developing systems for controlling recombination frequency during meiosis, a capability that will enhance the efficiency of plant breeding programs. Objective/Objectifs: The research will involve characterising Arabidopsis plants with altered DNA recombination potential resulting from mutation of DNA repair genes or their overexpression through development of transgenic lines. The characterisation will apply techniques already established in the lab and involve quantifying responses to DNA damaging agents, evaluating for defects in DNA metabolism (e.g. replication defects) and signal transduction processes, as well as defining altered growth and development. Candidate genes with pronounced effect on DNA repair capability will be applied in transgenic approaches to alter recombination potential in vegetative cells and during meiosis. Outcome expected/Résultats attendus: The initial research using Arabidopsis will identify genes and define strategies for use in manipulating recombination frequency in plants. These results will be applied to manipulate recombination frequency in crop species, particularly oilseed Brassica species, to accelerate plant breeding and enable direct optimisation of metabolic pathways via in vivo protein engineering. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship program—The postdoctoral intern will work in an established plant molecular biology lab conducting plant biology research in a work group consisting of experienced technicians and other post-doctoral researchers. The intern will participate in ongoing aspects of the research program and be responsible for an independent research project that is integrated with the lab’s research program. The research will involve a wide-range of molecular biology, biochemistry, cell biology and genetic techniques including: gene cloning and engineering; gene expression analysis; protein engineering; protein-protein interactions; subcellular localisation of proteins through fluorescent protein fusions; SNP analysis, development and characterisation of transgenic plants. Candidate qualifications—A Ph.D. in plant biology, preferably with experience in molecular biology, is required. Ability to work independently, with good oral and written communication skills in English is also necessary. (Update: January 28, 2009) Page 49 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Benefits to the candidate—The intern will develop comprehensive knowledge and skills in plant molecular biology transferable to a breadth of possible subsequent studies in applied and basic plant biology. (Update: January 28, 2009) Page 50 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Saskatoon_03 Back PROJECT TITLE / TITRE DU PROJET : Seed-based production of recombinant proteins Internship location in Canada/ Saskatoon Research Centre, Internship Duration/Durée du Emplacement du stage Saskatoon, Saskatchewan stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: RozwadowskiK@agr.gc.ca d’AAC) Kevin Rozwadowski Phone/Téléphone: 1-306-956-7692 Name of Research Centre/Nom du Centre de Recherche : AAFC-Saskatoon Research Centre Mailing address/Adresse postale du Centre : 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Kevin Rozwadowski, Recombination Biology Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Dwayne Hegedus & Derek Lydiate University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: Seed-based synthesis of recombinant proteins is a highly attractive system for production of industrial proteins (e.g. enzymes, fibres, etc.) due to the ease of scalability, transport and storage. However, attempts to accumulate novel proteins in seeds from transgenes have resulted in disappointing yields to-date. This is due to the evolutionary programming of seed development to channel much of metabolism in the developing embryo to accumulate native seed storage proteins (SSPs) which act as a reserve of sulfur and nitrogen for the germinating seed: this natural metabolic programming commits metabolic resources to produce the native SSPs instead of the desired introduced recombinant protein. Therefore, a practical approach to promote accumulation of recombinant proteins is to suppress the accumulation of the native SSPs. The most efficient way to achieve this in crop species with complex genomes encoding large SSP gene families is through expression of transcriptional repressors that directly inhibit expression of SSP genes. We have used genetic and molecular approaches to develop candidate repressors that are effective in suppressing SSP accumulation Objective/Objectifs: The research will investigate development and analysis of transcriptional repressors of SSP genes for application to inhibiting SSP accumulation in oilseed crucifer species. Initial research will be conducted using the Arabidopsis model system to characterise repressor candidates. This will involve engineering DNA binding proteins with protein domains encoding repressor motifs or chromatin-remodelling components. Gene promoters specific for the maturation stage of seed development will be identified. Transgenic Arabidopsis lines will be created using the promoter and repressor combinations and characterised for SSP accumulation. Outcome expected/Résultats attendus: The research in the Arabidopsis model system will identify repressor genes, promoters and strategies for inhibiting the accumulation of SSPs. These results will be applied to oilseed crucifer crop species to develop a platform for producing recombinant proteins in seeds to meet industry needs and provide an alternate revenue stream for producers. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Internship program—The postdoctoral intern will work in an established plant molecular biology lab conducting plant biology research in a work group consisting of experienced technicians and other post-doctoral researchers. The intern will participate in ongoing aspects of the research program and be responsible for an independent research project that is integrated with the lab’s research program. The research will involve a wide-range of molecular biology, biochemistry and genetic techniques including: gene cloning and engineering; gene expression analysis; protein engineering; protein-protein interactions; promoter characterisation; characterising embryo, seed and whole plant development and growth; generation and characterisation of transgenic plants. Candidate qualifications—A Ph.D. in plant biology, preferably with experience in molecular biology, is required. Ability to work independently, with good oral and written communication skills in English is also necessary. (Update: January 28, 2009) Page 51 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Benefits to the candidate—The intern will develop comprehensive knowledge and skills in plant molecular biology transferable to a breadth of possible subsequent studies in applied and basic plant biology. (Update: January 28, 2009) Page 52 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Saskatoon_04 Back PROJECT TITLE / TITRE DU PROJET : Formulation development for baculovirus Internship location in Canada/ Saskatoon Research Centre, Internship Duration/Durée du Emplacement du stage Saskatoon, Saskatchewan stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: hynesr@agr.gc.ca d’AAC) Russell K. Hynes Phone/Téléphone: 1-306-956-7638 Name of Research Centre/Nom du Centre de Recherche : AAFC-Saskatoon Research Centre Mailing address/Adresse postale du Centre : 107 Science Place, Saskatoon, SK, Canada S7N 0X2 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Drs. Russell K. Hynes and Martin Erlandson Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Dr. D. Korber, Department of Food and Bioproduct Science, Univ. of Saskatchewan Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: The cabbage looper (Trichoplusia ni) (Lepidoptera: Noctuidae) has become a serious pest of greenhouse vegetable (tomato, sweet pepper and cucumber) production in Canada due in part to the development of populations that are resistant to Bacillus thuringiensis (Bt) based bioinsecticides. Baculoviruses provide an attractive alternative to Bt products for cabbage looper control as they are highly infectious and typically host specific and therefore, compatible with IPM systems based on the use of a complex of insect biological control agents. The Baculoviridae are a diverse family of rod-shaped, occluded viruses that have circular dsDNA genomes ranging in size from 80 to 180 kb and are divided into two genera: the Nucleopolyhedrovirus (NPVs) and the Granulovirus (GVs). Baculoviruses, TnSNPV and TnMNPV, have been evaluated as biocontrol agents for T. ni in cole crops and deemed to have good potential for development as viral insecticides. Research studies conducted at AAFC Saskatoon Research Centre have indicated that strain AcMNPV may have greater utility then other baculovirus strains tested. However, a formulation technology for AcMNPV that promotes survival and application using conventional greenhouse delivery systems is lacking. Formulations are composed of i) an active ingredient, i.e. microorganism, microbial metabolite or natural product, ii) carriers, often inert materials used to support and deliver a high concentrations of the biopesticide and iii) adjuvants, compounds that promote and sustain the function of the biopesticide, provide protection from UV radiation, ensure attachment on the target, promote the spread and dispersal of the biopesticide and if required retain moisture or provide protection against desiccation. The scope of formulation types that can be investigated in this study can include, for example, wettable powders and microencapsulation technologies for these baculovirus. This project will require i) screening of formulation components for compatibility with survival and efficacy of AcMNPV with T. ni, ii) screening of formulation processing technologies such as extrusion, spheronization and fluidized bed drying, and iii) survival and efficacy testing of formulated AcMNPV following application using conventional greenhouse delivery systems. Objective/Objectifs: The objectives of this research program are to i) develop formulation and application technologies for biopesticides for insect pest management in greenhouse vegetable production, ii) promote the transfer of this technology to the agriculture industry sector. Outcome expected/Résultats attendus: Postdoctoral candidate will receive training in handling baculovirus, insect rearing, plant cultivation, and biopesticide formulation development including operating the following equipment extruder, spheronizer, fluidization bed dryer and particle sizer (Mastersizer 2000 laser scanning system). C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The internship program is for a post doctoral candidate at Agriculture and Agri-Food Canada and with potential for collaboration with scientists at the University of Saskatchewan. Agriculture and Agri-Food Canada, Saskatoon Research Centre (Update: January 28, 2009) Page 53 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC is located on the campus of the University of Saskatchewan (http://www.usask.ca/). The Research Centre is equipped with state-of-the-art equipment to carry out the research project described above. Candidates must have graduated from a recognized university with a Ph.D. or an equivalent degree with expertise in microbiology, entomology or plant pathology. The candidate must be proficient in the English language. Under the advice and guidance of your supervisors you will conduct research that addresses the needs of the project described above. (Update: January 28, 2009) Page 54 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Saskatoon_05 Back PROJECT TITLE / TITRE DU PROJET : Molecular characterization of wheat adaptation genes Internship location in Canada/ Saskatoon Research Centre, Internship Duration/Durée du Emplacement du stage Saskatoon, Saskatchewan stage: 24 months/mois Contact: Yong-Bi Fu Email/Courriel: fuy@agr.gc.ca Phone/Téléphone: 1-306-956-7642 Name of Research Centre/Nom du Centre de Recherche: AAFC-Saskatoon Research Centre Mailing address/Adresse postale du Centre: 107 Science Place, Saskatoon, SK, S7N 0X2 Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherché Supervisor/superviseur: (name and title/ nom et titre): Dr. Christiane Deslauriers, Science Director Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Ron DePauw, Semiarid Prairie Agricultural Research Centre University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Wheat is one of the most important staple crops worldwide. Wheat genetic improvement depends largely on genetic variability available in the adapted wheat gene pool. Molecular characterization of diverse wheat germplasm provides essential information for understanding the genetic basis of wheat adaptation and useful guidance to utilization of wheat germplasm for further genetic improvement. Recently, a number of wheat genes associated with adaptation to environment stresses such as drought, salinity, winter hardiness, early maturity, have been cloned. However, there is lack of detailed characterization of these adaptation genes in improved wheat gene pools. Research effort is warranted to characterize and categorize the unique adaptation alleles for sustainable wheat improvement to various environment stresses. Objective/Objectifs: The overall goal is to enhance the utilization and conservation of diverse wheat germplasm. The specific objectives are 1) to infer the genetic basis of wheat adaptation by characterizing adaptation genes and categorizing unique adaptation alleles and 2) to develop effective strategies for searching unique wheat germplasm of wide adaptation. Outcome expected/Résultats attendus: Information on wheat adaptation genes, a list of genetically unique wheat germplasm, and scientific publications. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The Internship Program: The candidate is expected to conduct molecular research supervised by the research scientist by planting wheat seeds in greenhouse, collecting leave tissue, extracting DNAs, performing sequence analysis of various adaptation genes, analyzing sequence data with advanced bioinformatics tools, and publishing scientific findings. Qualifications: The candidate should have a PhD degree in molecular biology from a recognized university, is proficient in English, and is motivated to do independent scientific research. Benefits to Student: Saskatoon Research Centre is a state of art plant biotechnology/ genomics research facility. The candidate will have the opportunity to gain research experience in plant genomics and bioinformatics. The candidate will also have the opportunity to interact with other research groups within and outside the centre through joint lab meetings, collaborations, workshops and conferences. (Update: January 28, 2009) Page 55 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Sherbrooke_01 Back PROJECT TITLE / TITRE DU PROJET : Ammonia recovery by volatilization and capture Internship location in Canada/ Dairy and Swine Research and Development Centre, Internship Duration/Durée du Emplacement du stage Sherbrooke, Canada stage: 12 months/mois Contact: Lucie Masse Email/Courriel: massel@agr.gc.ca Phone/Téléphone: 1-819-565-9171 ext. 132 Name of Research Centre/Nom du Centre de Recherche : AAFC-Dairy and Swine Research and Development Centre Mailing address/Adresse postale du Centre : 2000 COLLEGE STREET, PO BOX 90, STN LENNOXVILLE, SHERBROOKE, QC, CANADA J1M 1Z3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Lucie Masse, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Daniel Massé University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: Bioterre System Inc. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Wastes generated by large animal farms or slaughterhouses often contain excessive amounts of nutrients for the regional cultivated land base. Solid-liquid separators and biological reactors have been developed to concentrate phosphorous in the solid fraction. However, the liquid fraction generated by these technologies still contains most of the ammonia. Excess nitrogen can be processed in nitrification-denitrification bioreactors, but this system eliminates a valuable nutrient that could be used in feed and food producing regions. The wastage of nitrogen means that additional inorganic fertilizers must be produced, using an energy-intensive and greenhouse emitting process. On the other hand, the transport of manure and sludges is often excessively costly given the low nitrogen concentrations compared to chemically produced nitrogen fertilizers. Also, organic wastes may contain contaminants (e.g. pathogens) of volatile compounds (e.g. methane and CO2 precursors, NH3), that could produce undesirable emissions during transport and land applications. Our research team, composed of two research scientists, two technicians qualified in laboratory analysis, data acquisition systems and mechanical engineering, as well as graduate and undergraduate students, has been actively working in the area of nitrogen recovery. The project presented here would consist in developing a technology to volatilize ammonia in organic waste and capture it in a clean and transportable medium. An experimental set up has been installed in our laboratory and analytical procedures have been tested. We are now establishing the optimum conditions (pretreatment, pH, initial and final concentration, receiving solution, temperature, air velocity, air/liquid contact, etc.) for the process. The setup has been linked to membrane systems to create these optimum conditions. The laboratory also has anaerobic digesters that can provide treated manure and slaughterhouse sludge effluents. Objective/Objectifs: The ultimate objective of the project is the development of a technology to volatilize and capture ammonia from liquid manure, slaughterhouse sludges and anaerobic effluents. The end-produce would be a clean nitrogen fertilizer that could be sold to feed and vegetable farmers as an alternative to chemical nitrogen fertilizers. Outcome expected/Résultats attendus: The outcomes expected from the one-year internship would be: the elaboration of optimum conditions for the operation of the system a list of technical specifications for the design of a semi-commercial scale pilot the draft of a scientific paper C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The work of the intern would consist in: 1. familiarising him/herself with existing literature, based on a review already written by the project leader (Update: January 28, 2009) Page 56 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC 2. 3. 4. 5. 6. 7. writing experimental protocols if required, modifying or improving the existing experimental setup carrying the experiments – with the help the team technicians supervising the laboratory analyses of the samples analysing results and suggesting modifications to the procedures, protocols and experimental setup write up the draft for a scientific article to be published in a referee journal Qualifications: be able to read scientific articles in English and write protocols in French or English have a basic knowledge of waste composition and treatment systems, especially in the field of nutrient recovery have a good chemistry background Benefits to the intern Acquiring experience in the emerging field of nutrient recovery, which aim is to reduce consumption of nonrenewable resources and limit the leaching and emissions of contaminants in the environment. Acquiring experience in environmental research in a well equipped governmental laboratory working with laboratoryscale as well as commercial scale treatment systems. Developing technical knowledge of treatment systems such as membrane filtration, anaerobic digestion, and volatilisation/capture technologies. (Update: January 28, 2009) Page 57 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Sherbrooke_02 Back PROJECT TITLE / TITRE DU PROJET : Effect of vehicle design and transport distance on stress response and carcass and meat quality of three pig lines Internship location in Canada Dairy and Swine Research and Development Centre, Internship Duration/Durée du Sherbrooke, Quebec stage: 12 months/mois Contact: Luigi Faucitano Email/Courriel: faucitanol@agr.gc.ca Phone/Téléphone: 1-819-565-9174 ext. 237 Name of Research Centre : AAFC-Dairy and Swine Research and Development Centre Mailing address : 2000 COLLEGE STREET, PO BOX 90, STN LENNOXVILLE, SHERBROOKE, QC, CANADA J1M 1Z3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor: Luigi Faucitano, PhD, Bioproducts and Bioprocesses Other AAFC collaborators : Nicolas Devillers, PhD and Stephanie Torrey, PhD University collaborators : Linda Saucier (Laval University); Anna Bassols (University of Barcelona, Spain) and Matilde Piñeiro (University of Zaragoza, Spain) Industry partners: Breton Food B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue: The higher susceptibility to stress of the Piétrain breed results in increased transport mortality rate and inferior meat quality. However, it’s not sure whether the higher stress sensitivity is due to the high frequency of the so-called Halothane (HAL) gene in this genotype or to the breed itself. The major international pig genetic companies are selling terminal boars of 25-50% Piétrain genetics in order to take advantage of the high growth performance and carcass yield. However, the use of pigs of P genetics is more challenging in North America given the difficult transport conditions (vehicle design, distance and climate). The transport factor contributed by 19% to the mortality rate variation reported in a transport survey in Canada. Poor vehicle design may explain the effects of the transporter on these mortality losses. In Canada pigs are mostly transported to slaughter using pot-belly trailers. This trailer design is not considered suitable for swine transportation as it has a large number of steep ramps resulting in more stress during loading and unloading with clear consequences on the incidence of deads-on-arrival and downers. Other common vehicle for swine transportation is the flat deck trailer. Compared to the pot-belly trailer, the flat deck model is shorter to load and unload and produces less casualties. However, like the pot-belly trailer, its effects on response and meat quality of pigs of different genetics transported at different travel distance were never evaluated. Objectives : 1) Study of the effects of vehicle design and transport distance on behaviour, physiology and carcass and meat quality of pigs; 2) Study of the response to transport stress in pigs of Halothane-free Pietrain pigs and its effects on carcass and meat quality variation Outcome expected: The interest of swine producers towards pigs of Piétrain genetics is based on their higher feed efficiency, faster growth rate and better carcass traits. However, their use is limited because of the high stress-susceptibility leading to two-fold higher transport mortality and up to four times higher incidence of PSE pork. The recommendations for the preslaughter management of Piétrain pigs arising from this study will be useful to producers who want to take advantage of the high performance and carcass value of this genotype and, at the same time, limit the losses caused by death in transit or PSE pork. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. A post-doc student in Animal Science would qualify for this program. The intern will participate in the sample and data collection at the farm and slaughter plant, laboratory analysis (blood analysis, body temperature and behavioural data handling). The intern will get acquainted with the techniques for the assessment of animal welfare before slaughter and the online assessment of carcass and meat quality traits. (Update: January 28, 2009) Page 58 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Sherbrooke_03 Back PROJECT TITLE / TITRE DU PROJET : Factors influencing variations in milk protein constituents Internship location in Canada/ Dairy and Swine Research and Development Centre, Internship Duration/Durée du Emplacement du stage: Sherbrooke, Québec stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: Daniel.Ouellet@agr.gc.ca d’AAC) Daniel Ouellet Phone/Téléphone: 1-819-565-9171 ext. 209 Name of Research Centre/Nom du Centre de Recherche : AAFC-Dairy and Swine R&D Centre Mailing address/Adresse postale du Centre : 2000, College street P.O. Box 90 STN Lennoxville, Sherbrooke (Québec) Canada J1M 1Z3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Daniel Ouellet, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Kevin Wade, Ph.D. Professor, McGill University; René Lacroix, Ph.D. Professional associate, McGill University Industry partners/Partenaires industriels: Daniel Lefèbvre, Ph.D. Director of R&D, Valacta B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: A better control by dairy producers on milk components that are produced at the farm is imperative for the dairy industry to continue to grow. Factors that influence the distribution of the different fractions of protein and lipids are numerous and include climate, breed, feed, genotype, stage of lactation, parity, etc. The focus of this project will be on milk protein constituents, for which regional and seasonal variations have been observed in past studies. In order to better understand such variations and factors influencing it, more than 900 milk samples were collected on about 30 dairy farms in Quebec between 2005 and 2008. The samples were collected on test day on farms that were enrolled in a Dairy Herd Improvement (DHI) program with Valacta inc. The DHI program involved the collection of milk recording data on each cow (e.g. days in lactation, disease), feed recommendations and feed chemical analyses, and farm characteristics. Samples were collected for individual cows and from bulk tanks (25% of the samples). Milk samples were analyzed in details (content and profiles) for fat and protein. Objective/Objectifs: The goal of the present project is to analyze factors that could lead to variations in milk protein constituents especially protein, casein, urea, and whey, using results from milk sample laboratory analyses combined to DHI production and feed data. Outcome expected/Résultats attendus: The first outcome from this work it the establishment of mathematical relationships as the basis of a model relating feed and management data to milk protein profiles. The proposed approach differs from previous studies in the sense that the model will be based on data routinely collected on farms by a DHI. This allows for the application of the model to potentially all farms using DHI feed services. In turn, this leads to two important outcomes: 1) the extension of the study by using the model with the data from all those farms (i.e., several thousands in the Province of Quebec), to predict and analyze variations of the protein profiles, 2) the eventual use of the model by DHIs to help dairy producers controlling the protein profiles of the milk that they produce through management practices, including feed ration formulation. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The results of milk sample analyses (e.g., protein profiles) and the data provided by Valacta has been organized into a central MySQL data base. The data can be accessed with analytical tool such as SAS. Analyses of this type of data is challenging and required an expertise that has been traditionally mastered by animal scientists in the area of quantitative geneticist, and not so much in the area of dairy cattle nutrition. Traditionally, research by this second group of researchers has been handled mostly through physical experimentation. In this project, it is proposed to apply a quantitative approach in an area that includes dairy nutrition, which will be the focus of the research of the postdoctoral fellow. The postdoctoral candidate needs a strong interest and skills in mathematics/statistics and computer literacy. At the end of the project, the candidate will have developed deep (Update: January 28, 2009) Page 59 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC skills in handling and analysis of large data sets. The candidate will benefit from the expertise in feed nutrition and milk components at the AAC experimental station in Lennoxville, quantitative analysis from researchers at McGill University (Macdonald Campus), and DHI data characteristics from Valacta inc (Ste-Anne-de-Bellevue). The skills that will be developed are increasingly in demand in most research areas, for example in genomics and proteomics. (Update: January 28, 2009) Page 60 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_SJSR_01 Back PROJECT TITLE / TITRE DU PROJET : Use phytochemical as marker to select strawberry and raspberry lines for processing (juice & fruit wine) Internship location in Canada/ McGill University, St. Anne de Bellevue, Quebec and Internship Duration/Durée du Emplacement du stage AAFC-Horticultural Research & Development Centre, St. stage: 12 months/mois Jean sur Richelieu, Quebec Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: khanizadehs@agr.gc.ca d’AAC) Shahrokh Khanizadeh Phone/Téléphone: 1-450-515-2058 Name of Research Centre/Nom du Centre de Recherche : AAFC-Horticultural Research and Development Centre, Mailing address/Adresse postale du Centre : 430 Boul Gouin, St Jean sur Richelieu, QC, Canada, J3B 3E6 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherché Supervisor/superviseur: (name and title/ nom et titre) Dr. Shahrokh Khanizadeh, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Marie-Thérèse Charles University collaborators/Collaborateurs universitaires: Dr. Katrine Stewart Industry partners/Partenaires industriels: Lareault Nurseries and Phytoclone B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique:: Strawberries and raspberries are one of the leading small fruits in Canada. The major part of the production is consumed fresh, and a large part of it is processed. Interest in polyphenolic compounds and antioxidant activity of fruits has been very high in recent years owing to their contribution to sensory quality of fruit, juice, dried fruits, cider, frozen fruit treat, dried fruit bar, and fruit roll ups and their possible relationship with shelf life and disease susceptibility. For the past few years, growing interest has been devoted to the phytochemical content of the fruits and specific attention has been given to ellagic acid and flavonoids. These compounds not only are known to influence quality, acceptability and stability of foods by acting as flavorants, colorants or antioxidants but also act as defence mechanisms and also play an important role in senescence and the ageing process and shelf life. Substantial variation has been observed in Strawberries and Raspberries for biochemical content. As underlined by several authors, the antioxidant profile of fruits need to be considered in breeding programs, along with field performance and responses of the selections to cultural practices as well as harvest and post-harvest storage strategies in order to bred a locally adapted disease resistant line. The use of chemical composition as marker assisted in breeding allows plant breeders to significantly accelerate the speed of a natural (traditional) plant breeding program, without exposure to the unpredictable health and environmental risks associated with genetic engineering techniques. The aim of this proposal is to identify the factors controlling fruit quality specially for processing and develop a methodology and a model in order to reduce the time from crossing to naming to select disease resistant lines with high fruit quality. Objective/Objectifs: Short term 1. Study and quantify the individual phenolic compounds in selected lines from an existing population. 2. Determine the affect of phenolic content and total antioxidant capacity on fruit quality. 3. Evaluate the affect of selected environmental factors on chemical and phytochemical composition. 4. In vitro evaluation of selected antioxidant and their interaction on selected diseases progress. Long term 5. Development of a model to use chemical composition/phytochemical as marker to select high quality disease resistant lines for processing (juice, dried fruits, cider, Frozen Fruit Treat, dried fruit bar, and fruit roll). Outcome expected/Résultats attendus: 1. The out come will supply breeders with a tools, such as chemical markers linked to fruit quality and disease resistance, in order to improve selection developing distinct strawberry and raspberry lines for processing e.g. juice, fruit wine, dried and frozen fruit treat, fruit bar and roll ups. 2. Develop a new raspberry or strawberry lines suitable for processing useful to both countries 3. World-class scientific publication (Update: January 28, 2009) Page 61 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Agriculture and AgriFood Canada and McGill University are both prestigious research and teaching institution, well known in Canada and abroad. It offers post graduate and post doctorate training to selected Ph.D. level. This program is emphases on chemical composition in breeding as marker to identify lines for processing. The internship not only educates the candidate academically but also allow him/her to get familiar with research conducted at federal research center. The proposed practices not only are useful to Canadian public and industry but also will be applicable to other countries in agriculture. The candidate has the opportunity to work with senior scientists/academic with many years of experience and extended number of publication in this field. More specifically the candidate will benefit by improving his experience in fruit breeding and genetics, acquiring in-depth understanding of bio-chemical and will be involved in preparing and submitting scientific publications while working within a research team including industry, university and research centers. (Update: January 28, 2009) Page 62 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_SJSR_02 Back PROJECT TITLE / TITRE DU PROJET : Impact of climate change and variability on the productivity of field grown vegetable crops: A bioclimatic modelling approach Internship location in Canada/ Horticultural Research & Development Centre, Internship Duration/Durée du Emplacement du stage St. Jean sur Richelieu, Quebec stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: Gaetan.Bourgeois@agr.gc.ca d’AAC) Gaétan Bourgeois Phone/Téléphone: 1-450-515-2017 Name of Research Centre/Nom du Centre de Recherche : AAFC-Horticultural Research and Development Centre, Mailing address/Adresse postale du Centre : 430 Boul Gouin, St Jean sur Richelieu, QC, Canada, J3B 3E6 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherché Supervisor/superviseur: (name and title/ nom et titre) Gaétan Bourgeois, Ph.D., Bioclimatology and Modelling Other AAFC collaborators/Autre collaborateurs à AAC: Dominique Plouffe, B.Sc.; Danielle Choquette, B.Sc.; Nathalie Beaudry (Computer Specialist); Élizabeth Pattey, Ph.D. International collaborators/Collaborateurs internationaux : Marie Launay, Ph.D. and Nadine Brisson, Ph.D. (INRA, Avignon, France) University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: Diane Chaumont (Climate change specialist, Ouranos, Montreal) B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Many crop growth simulators have been developed for field crops such as wheat, soybean, corn, etc. They have been used mostly to improve our understanding of crop physiology and to some extent as decision support tools at the farm. One of these tools is the software STICS, which was developed in France to simulate the effect of climate, soil, and crop management on the production and the environment. It was conceived as a generic model, able to adapt easily to various kinds of plant. Many physiological processes are included in this simulator, which makes it quite mechanistic by its internal complexity. Another crop simulator is being developed by the research team in bioclimatology and modelling of AAFC. The simulator proposed, named Sim2Cult, will also be generic but its internal structure will be much simpler by limiting the physiological processes to the most critical ones in the prediction of crop productivity. The two crop simulators indicated previously were adapted mostly for field crops (i.e. wheat, maize, soybean, etc.) and limited adaptations have been done for field vegetable crops. Since 1990, in their studies on disease and insect forecasting, the research team in bioclimatology and modelling of AAFC has accumulated many data sets on the phenology, growth, and yield of a number of vegetable crops, namely carrot, onion, and celery. They also have access to historical climate data and future climate scenarios that are required inputs to crop growth simulators. All these biotic and abiotic data are key elements for the adaptation of crop simulators to these vegetable crops. Objective/Objectifs: 1) Adaptation of two crop growth simulators (i.e. STICS and BDGSim) to carrot, onion, and celery; 2) Using different simulation techniques, improve our understanding of the impact of climate change and variability on vegetable crop productivity; 3) Propositions of crop management strategies to help the agricultural sector in their adaptation to climate change and variability. Outcome expected/Résultats attendus: Adaptation of two crop growth simulators (i.e. STICS and BDGSim) to a number of vegetable crops; Evaluation of the predictions with observed field data; Simulations over many historical climate data and future climate scenarios to assess the impact of climate change and variability on vegetable crop productivity; Generation of important information to help the agricultural sector in their adaptation to climate change. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. (Update: January 28, 2009) Page 63 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC Description of internship program: The PDF intern will be in charge of 1) reviewing the litterature, 2) adapting existing bioclimatic crop simulators (i.e. STICS and Sim2Cult) to a number of vegetable crops, 3) evaluating the predictions with observed field data, 4) simulating future crop productivity from climate change scenarios, and 5) preparing reports and/or first draft of scientific articles. Expected qualifications: Good knowledge of crop physiology and productivity; Basic knowledge in crop and/or pest bioclimatic modelling; Efficiency in using computer software and handling large data sets; Interest in impacts of climate change and variability on agriculture; Interest in mathematics. Benefits to the intern: Scientific experience in bioclimatology and modelling research; Training with specialized software developed for crop and pest modelling; Active participation in meetings of the research team where many aspects of bioclimatic modelling are discussed; International collaborations and contacts in bioclimatology, crop modelling and climate change; Possibility of co-authorship on a number of scientific articles; Improved understanding of the expected impact of climate change on crop productivity and associated pests. (Update: January 28, 2009) Page 64 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/NO DU PROJET: 2009_StHyacinthe_01 Back PROJECT TITLE / TITRE DU PROJET : Overproduction of extremophilic bacterial enzymes (lactase and lipase) for the industrial applications Internship location in Canada/ Food Research and Development Centre, Internship Duration/Durée du Emplacement du stage Saint-Hyacinthe, Québec stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: leeb@agr.gc.ca d’AAC) Byong H. Lee Phone/Téléphone: 1-450-773-1105 Name of Research Centre/Nom du Centre de Recherche : AAFC-Food and Research Centre Mailing address/Adresse postale du Centre C : 3600 Blv. Casavant West, St-Hyacinthe, QC, Canada J2S 8E3 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Byong H. Lee, Principal Research Scientist, Bioproducts and Bioprocesses Other AAFC collaborators/Autre collaborateurs à AAC: Drs. Normand Robert and Yves Arcand University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Although the genetics and molecular tools of common bacteria exist, knowledge is lacking behind with extremophilic bacteria. We have screened several new strong promoters to express high level of enzyme expression and thus after the molecular tools are available and molecular mechanisms are elucidated, a more rational approach to the overproduction of industrially important enzymes and bioactive molecules could be possible. We have successfully over-expressed more than 1,000 folds of (1) an aminopeptidase of Lactobacillus rhamnosus which has many applications in biotechnology and foods, (2) lipase of Rhizomucor miehei (In-house invention, 2007), (3) esterase of Lactobacillus casei (Choi et al., 2004. Appl. Env. Microbiol. 70:3213-3221), lactase of Bifidobacterium (Hung et al.2001. Appl. Env. Microbiol. 67:4256-4263) and some have scaled-up the fed-batch process in 150 liter fermentor, very little works have been carried out with the over-expression enzymes of extremophilic bacteria which can grow at cold temperature (below 10 oC) or above 100oC, that are very useful for the industrial applications in detergent, food, and biotechnology. Besides the application of hydrolytic lactase for (1) reducing lactose content in milk and lactose intolerance in dairy products, (2) producing whey syrup sweeteners from lactose, lactase can produce (3) galacto-oligosaccharides, GOS, as bifidus factors'-promoting the growth of desirable intestinal microflora. Lipases are endowed with a substrate specificity surpassing that of any other known enzyme. This confers on the lipases an application potential that is literally boundless (Jaeger and Eggert, 2002. Curr Opin. Biotech., 13:390-397). Objective/Objectifs: The goal of this work is to (1) investigate the molecular basis of enzyme expression from extremophilic bacteria, and (2) to apply this knowledge for the production of recombinant enzymes and bioactive molecules economically important. The long term research program is to advance our understanding of gene function and expression of extremophilic bacterial enzymes and the effects of these activities on the production of bioactive molecules. Outcome expected/Résultats attendus: The extremophilic bacteria derived enzymes (lactase and lipase) which are either cold temperature active or thermostable can have many applications in bioindustries. After the overexpression system is developed, for the application of industrial scale, we will also find a fusion system that does not need expensive materials for the purification. One of the attractive protein that be used as fusion protein is exoglucanase Cex (47 kDa) from Cellulomonas fimi. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Previously Dr. Jenny Blamey (jblamey@bioscience.cl), Research Director and me. Olivier Rickmers, President (orickmer@bioscience.cl) requested me to collaborate on the extremophilic enzymes. This non-profit industry has expertise in the extremophilic bacteria and has the best known sources of these bacteria in the world. The expected student qualification should be a molecular biologist and biochemistry. If possible the funding for two years will be ideal for the project, but one year for the first trial will be fine as well. (Update: January 28, 2009) Page 65 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_StJohns_01 Back PROJECT TITLE / TITRE DU PROJET : Bioreactor micropropagation and genetic fidelity in berry crops Internship location in Canada/ Atlantic Cool Climate Crop Research Centre, Internship Duration/Durée du Emplacement du stage St. John’s, New Foundland stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: debnaths@agr.gc.ca d’AAC) Samir C. Debnath Phone/Téléphone: 1-709-772-4788 Name of Research Centre/Nom du Centre de Recherche : AAFC-Atlantic Cool Climate Crop Research Centre Mailing address/Adresse postale du Centre : P.O. Box 39088, 308 Brookfield Road, St. John’s, NL A1E 5Y7, Canada Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) : Dr. Samir C. Debnath, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: Cris Lester, Lester’s Produce Inc., St. John’s, NL, Canada; Mike Rabinowitz, Organic Farm, Portugal Cove, St. Phillips, NL, Canada. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: An improved understanding of the important role of dietary fruit in maintaining human health has led to a dramatic increase of the global berry crop production and berry demand in recent years. While berry fruit have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. Of particular significance has been the evolution of the technology permitting multiplication of berry plants through bioreactor micropropagation. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem and strategies have been developed in order to reduce the variation to manageable levels. Molecular markers such as RAPDs, RFLPs, AFLPs, DAFs, SCARs, SSRs, and ISSRs have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. The current program will focus on the bioreactor systems used for in vitro culture of berry crops (raspberry and strawberry) and employment of molecular markers in micropropagated berry plants for the assessment of genetic fidelity, uniformity, stability, and true-to-typeness among donor plants and tissue culture regenerants. Objective/Objectifs: 1. To develop and improve in vitro techniques for strawberry and raspberry micropropagation using bioreactors. 2. To use genetic fingerprinting protocol to monitor trueness-to-type of micropropagated berry plants and to characterize novel somaclones in strawberry and raspberry micropropagules. Outcome expected/Résultats attendus: 1. Best cost effective propagation method using bioreactor will be developed. 2. Protocol to monitor trueness-to-type of micropropagules and somaclones will be developed. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The internship program will consist of culturing raspberry and strawberry explants on culture media, regeneration and proliferation of microshoots, their rooting under in vitro condition and acclimatization of in vitro-derived plants under greenhouse and field conditions. DNA isolation, PCR analysis, gel electrophoresis and PCR data analysis will be done following standard procedures. The expected student qualifications will be a Ph.D. degree in biology, botany, biochemistry or related science with experience in plant tissue culture and/or molecular analysis. The student will learn new techniques on in vitro and molecular analyses and will be a co-author of peer-reviewed journal articles. (Update: January 28, 2009) Page 66 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Summerland_01 Back PROJECT TITLE / TITRE DU PROJET : Diagnostic Technologies and Management Strategies for Trunk Disease of Grapevines in British Columbia Internship location in Canada/ Pacific Agriculture Research Centre, Internship Duration/Durée du Emplacement du stage Summerland, British Columbia stage: 24 months/mois Contact: Dan O’Gorman Email/Courriel: Daniel.OGorman@agr.gc.ca Phone/Téléphone: 1-250-494-6364 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agriculture Research Centre Mailing address/Adresse postale du Centre : 4200 Hwy. 97, Box 5000, Summerland, BC, Canada V0H 1Z0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Daniel O’Gorman (Biologist), Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Peter Sholberg, Karen Bedford, Paula Haag University collaborators/Collaborateurs universitaires: Doug Gubler (UC Davis) Industry partners/Partenaires industriels: BC Wine Grape Council B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Vine decline includes a number of destructive trunk and root diseases of grapevines which are commonly found throughout the grape growing regions of the world. Some of the most important of these diseases are Esca, Botryosphaeria canker and Black Foot. All of these diseases reduce yields and increase production costs through the removal of diseased vines, re-planting with new vines, and any control measures (cultural and chemical) undertaken to prevent or lessen disease severity. That being said, in Canada there are no chemicals registered for grapes that show effective control of these pathogens. In addition, each of these diseases is caused by multiple species of fungi which complicates the ability to obtain an accurate diagnosis. The pathogens can also effect the vines in what is known as a disease complex (more than one pathogen attacking the vine) making it potentially difficult to identify the correct management strategies. Therefore the development of an efficient and accurate diagnostic tool and establishment of control strategies for regional environmental conditions are required to manage these diseases effectively. Objective/Objectifs: 1. (a) Development and validation of molecular diagnostic DNA macro-array for rapid and accurate detection of the numerous fungal species responsible for each of these diseases (Esca, Botryosphaeria canker and Black foot). (b) Assessment of the DNA array as a diagnostic tool to test for infection in mature vines from vineyards, as well as nursery stock samples to ensure clean material prior to planting. 2. Evaluate disease management strategies: potential chemical and biological controls, as well as cultural practices suited for the unique local environmental conditions. Suitable sites have been identified for Black foot and Botyosphaeria decline disease management trials and we will also attempt to find appropriate sites to conduct trials for the management of Esca infected vines. 3. Continued monitoring of local vineyards to: (a) confirm the identity of the species responsible for each of these trunk diseases in BC; (b) identify disease symptoms, severity and spread; and (c) determine if these diseases are primarily occurring, individually or collectively as a disease complex. Outcome expected/Résultats attendus: This multi year project will: (a) help identify which of the species exist in BC vineyards; (b) develop a comprehensive DNA macro-array capable of detecting one or more pathogens in a single diagnostic test in field and nursery settings; and (c) help identify control measures (biological, chemical and cultural) which will be effective in British Columbia’s unique climate C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. During the 24 month internship the candidate will be involved in: (a) the propagation and planting of the test vineyard; (b) (Update: January 28, 2009) Page 67 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC drafting of the experimental design and conducting both greenhouse and field trials; (c) work with the PI on the design, development and validation of probes for the DNA array; and (d) oversee the disease survey and pathogen isolation and identification. To accomplish the goals successfully, the project will require a team effort in both lab and filed work,. However, depending on the qualifications and abilities the candidate, he or she may assume a lead role in as many facets of the projects as they can manage. The candidate will gain experience with diagnostics and management strategies of trunk diseases of grapevines, an important crop in both Canada and the intern’s home country. They will also gain experience in the design of molecular diagnostic tools which are becoming increasingly important in plant pathology. The candidate should have: (a) a strong background and experience in plant pathology and be comfortable with both lab and filed work. (b) a good understanding of molecular biology with practical experience with molecular lab techniques being an asset; and (c) a positive attitude and work well as part of a team. (Update: January 28, 2009) Page 68 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Summerland_02 Back PROJECT TITLE / TITRE DU PROJET : Evaluation of the breadth and durability of engineered resistance to Plum pox virus based on the induction of RNA silencing Internship location in Canada/ Pacific Agriculture Research Centre, Internship Duration/Durée du Emplacement du stage Summerland, British Columbia stage: 24 months/mois Contact: Helene Sanfacon Email/Courriel: SanfaconH@agr.gc.ca Phone/Téléphone: 1-250-494-6393 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agri-Food Research Centre Mailing address/Adresse postale du Centre : PO Box 5000, 4200 Highway 97, Summerland, BC, Canada V0H 1Z0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: Dr. Helene Sanfacon, Bioproducts and Bioprocesses Other AAFC collaborators/Autre collaborateurs à AAC: This proposal is in the context of a large collaborative project aimed at engineering resistance to Plum pox virus (PPV) in peach. AAFC collaborators on the PPV project are: Drs. Dan Brown, Lining Tian and Aiming Wang (AAFC-London), Dr. Antonet Svircev (AAFC-Vineland) and Dr. Brian Miki (AAFC-Ottawa). The proposed research will be conducted in the Sanfacon laboratory in Summerland. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Durable eradication of PV in Canada will only be possible if elimination of infected plants in the field is accompanied with approaches to prevent future reintroduction of the virus. The generation of PPV-resistant peach germlines is necessary to accomplish this objective. We have developed strong resistance to PPV in model herbaceous hosts and in plum lines (Zhang et al, 2006. Can. J. Plant Pathol. 28, 263) . The resistance is based on the induction of PPV-specific RNA silencing in plants. Because of the high level of resistance obtained, this approach is the most promising technology for delivery to the industry by 2011. Regulatory approval of transgenic PPV-resistance requires supporting research to document the stability and breadth of the resistance Although strong resistance to the Canadian isolate of PPV was demonstrated, further research is required to study the breadth of the resistance to other PPV isolates. In collaboration with Dr. Ravelonandro (INRA-Bordeaux, France), we have confirmed that transgenic lines developed in Canada are resistant to a collection of European PPV isolates. However, the strength of the resistance varies somewhat with the isolate considered. RNA silencing is a natural antiviral resistance mechanism in plants that directs degradation of the viral genome in a sequence-specific manner. Many (but not all) plant viruses are known to encode proteins that can suppress RNA silencing. To evaluate the breadth and stability of the PPV resistance, it is important to determine the relative ability of various PPV isolates to evade or suppress the engineered resistance. Because other peach viruses are sporadically present in peach orchards in Canada, we also want to determine whether they can suppress RNA silencing. Objective/Objectifs: - Compare susceptibility of European and Canadian PPV isolates to RNA silencing in transgenic lines using sensor assays (portion of viral genome fused to fluorescent protein for visual detection of silencing and accelerated resistance assays); i.e. determine whether European PPV isolates can transiently evade silencing. - Evaluate silencing suppression activity in European and Canadian PPV isolates. - Continue an in-depth study of the interaction of other peach viruses with the RNA silencing pathway (see Jovel et al, 2007. J. Virol. 81, 12285-12297 for results obtained to date). Outcome expected/Résultats attendus: - Supporting documentation regarding the strength, breadth and stability of PPV-resistance for regulatory acceptance and commercialization - Increased understanding of the interaction between peach viruses and the plant RNA silencing pathway for eradication of PPV and improved management of other viruses (Update: January 28, 2009) Page 69 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. Funding for this research has been secured until April 2011. Dr. Sanfacon is a recognized leader in the field of molecular plant virology and has demonstrated expertise in the proposed research as evidenced by publications in high-impact international scientific journal. The research station is well equipped for research in molecular plant virology. Dr. Sanfacon leads a team of technicians, post-doctoral fellows (PDF) and Ph.D. students offering a dynamic environment for post-doctoral research. The research proposed will allow the student to develop expertise in an exciting, leading-edge field of research. Although the proposed research is aimed at the study of plant viruses occurring in Canada, the technology and expertise developed by the applicant will be directly applicable to the study and management of plant viruses in the home country. The PDF applicant must have previous experience in molecular plant biology and must have a Ph.D. in molecular biology or biochemistry from a recognized university. Previous experience with plant viruses would be an asset but is not required. The applicant must have a good command of the English language and must be prepared to work well with a team of researchers coming with various backgrounds and cultures. (Update: January 28, 2009) Page 70 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Summerland_03 Back PROJECT TITLE / TITRE DU PROJET : Biological control of orchard insect pests Internship location in Canada/ Pacific Agriculture Research Centre, Internship Duration/Durée du Emplacement du stage Summerland, British Columbia stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: Joan.Cossentine@agr.gc.ca d’AAC) Joan Cossentine Phone/Téléphone: 1-250-494-6366 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agri-Food Research Centre Mailing address/Adresse postale du Centre : 4200 Highway 97, Box 5000, Summerland, BC, Canada, V0H 1Z0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Joan Cossentine, Research Scientist, Environmental Health Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Gary Judd University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: BC Fruit Growers Association, Okanagan-Kootenay Sterile Insect Release Program B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Apple and pear orchards in British Columbia, Canada have benefited from the worlds largest area-wide control program for the key apple pest, the codling moth, for the last 15 years. The program is based on the release of sterile males, however integrates other codling moth control strategies including mating disruption and the codling moth granulovirus. Without the use of broadspectrum chemical insecticides, the introduction or use of parasitoids may be justified to supplement fruit pest suppression. The exotic codling moth parasitoid, Mastrus ridibundus, is being considered for introduction as it shows excellent potential to find and suppress low residual codling moth populations which are characteristic of area-wide control orchards. The program maintains a mass codling moth rearing facility that could be used to rear parasitoids however the technology and economics need to be developed and evaluated as well as a thorough field efficacy evaluation of parasitoid releases. Integrating biological controls makes the possibilities for pest suppression more complex. Parasitoids not only suppress insect pest populations by parasitizing and killing hosts, but have also been shown to enhance entomopathogenic biological control agents, by mechanically transmitting them to host larvae. Ichneumonid parasitoids, such as Mastrus species also inject a polydnavirus into host larvae to suppress their immune response. The suppression of the host’s immune system has been recorded to enable baculoviruses to cause virus-induced mortality in parasitized hosts. Wild orchard codling moth populations could experience previous exposure to sublethal concentrations of the codling moth granulovirus as an applied biopesticide or as a sublethal or latent infection. Prior viral infection of host larvae has also been shown to suppress the development of parasitoids. Consequently, the release of Mastrus ridibundus for the suppression of codling moth in British Columbian orchards may have the potential to augment or be suppressed by the use of the codling moth granulovirus. Molecular tools have been developed to identify the codling moth granulovirus. Objective/Objectifs: To determine if the mass-rearing and release of Mastrus ridibundus are an efficacious and economical pest management strategy to suppress hosts within an area-wide codling moth control program. Outcome expected/Résultats attendus: - Development of codling moth based mass-production technology for Mastrus ridibundus - Laboratory evaluation of the interaction of parasitoids with codling moth granulovirus - Field evaluation of strategic parasitoid releases assessing established parasitism, impact on host population densities, - and fruit damage suppression - Publication of all results C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The post doctorate candidate would be expected to have knowledge in the areas of entomology, biological control, and molecular biology and have experience working in the laboratory and field. Experience and knowledge in the area of (Update: January 28, 2009) Page 71 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC invertebrate pathology and parasitology would be helpful. The successful candidate will work in a biological control laboratory within a large, well equipped federal research facility with a team conducting research on the suppression of pests within temperate fruit orchards. The intern will become trained in the integrated pest management of lepidopteran apple orchard pests within the area-wide control program in British Columbia, develop skills in the mass production of both codling moth and parasitoids, gain experience in bioassay techniques, polymerase chain reaction skills for testing for the presence of a granulovirus, gain knowledge in the pathogenesis of the codling moth granulovirus and gain field experience in parasitoid release and assessment trials in Canadian orchards. Access to local commercial orchards will be arranged to carry out field trials. The knowledge obtained on the biological control of the codling moth may be applied to codling moth management within orchards of the intern’s home country. The knowledge obtained in all aspects of the program will potentially benefit the rearing, release, and evaluation of orchard parasitoids and our understanding of how they interact with pathogens. (Update: January 28, 2009) Page 72 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Summerland_04 Back PROJECT TITLE / TITRE DU PROJET : Landscape ecology and genetics of invasive pests in orchards Internship location in Canada/ Pacific Agriculture Research Centre, Summerland, British Internship Duration/Durée du Emplacement du stage Columbia and either University of British Columbia, stage: 12-24 months/mois Okanagan at Kelowna, B.C. or University of Western Ontario, London, Ontario Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: howard.thistlewood@agr.gc.ca d’AAC) Howard Thistlewood Phone/Téléphone: 1-250-494-6419 Name of Research Centre/Nom du Centre de Recherche : AAFC-Pacific Agri-Food Research Centre Mailing address/Adresse postale du Centre : 4200 Highway 97, Box 5000, Summerland, BC, Canada, V0H 1Z0 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Howard Thistlewood, Research Scientist, Environmental Health (Website/site Web: http://www4.agr.gc.ca/AAFC-AAC/display-afficher.do?id=1181923175739&lang=e) Other AAFC collaborators/Autre collaborateurs à AAC: Grace Frank, Coordinator of Geomatics (GIS) Unit, PARC; Scott Smith, Supervisor of Geomatics (GIS) Unit, PARC; Other partners in related Abase project, AAFC and JK Institute, Germany, or a possible continued IAEA collaboration. University collaborators/Collaborateurs universitaires: Prof. Jason Pither (Biology), or Prof. Sylvia Esterby, Paramjit Gill (Statistics) or Prof. Rebecca Tyson (Mathematics), at University of British Columbia – Okanagan, in Kelowna, B.C.; Prof. Nusha Keyghobadi (Biology), University of Western Ontario, in London, ON Industry partners/Partenaires industriels: Okanagan-Kootenay Sterile Insect Release Program, B.C.; BC Fruit Growers Association, Okanagan Tree Fruit Company, and others. B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Of value is that PARC Summerland is the only centre in Canada with significant research on fruit flies, the only Canadian centre for sustainable management of fruit pests in an arid area, unique in AAFC in having a state-of-the-art GIS unit for research, and has the largest dataset in Canada on physical topography, land use, micro-climates, and associated spatiotemporal insect counts. The projects in the Thistlewood lab typically involve academic collaborators at two sites per year and provide solutions to a variety of questions of interest to national quarantine agencies and large pest control projects, worldwide. We are bringing new analytical techniques and higher mathematics to bear on the data and have a good publishing record with enthusiastic collaborators. Together, a synergy may be developed that enhances skills of scholars and benefits colleagues in university or government laboratories. Objective/Objectifs: The project is part of a collaborative study on area-wide management of pest insects, to enable the sustainable and safe production of horticultural crops in a region of varied land use and varied terrain. The project is measuring insect movement and applying mathematical and statistical technology, geographic information systems and molecular microsatellite DNA tools, on fruit flies and moths. It supports a cooperative industry-government $60 million area-wide management program that has greatly reduced harsh insecticide use. Outcome expected/Résultats attendus: Understanding of movement and ecology of invasive insects in highly varied terrain and landscapes, or of micro-climates within a mountainous region, or of new molecular or GIS technology to enhance sustainable horticulture. One or two peerreviewed papers per year. Related publications have appeared in Acta Horticulturae, Biological Control, Canadian Entomologist, Canadian Journal of Statistics, Canadian Journal of Zoology, Ecological Modelling, Entomologia Experimentalis et Applicata, Environmental Entomology, Experimental & Applied Acarology, Insect Molecular Biology, Journal of Applied Ecology, Journal of Theoretical Biology, Landscape Ecology, Molecular Ecology Resources, and Pest Management Science. The Universities of British Columbia and of Western Ontario are highly rated within Canada and in international rankings. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. (Update: January 28, 2009) Page 73 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC The scholar will study existing projects and methods, when planning with Thistlewood and collaborator(s). Field-work in the Summer or Autumn at PARC Summerland, and in Winter conduct the analytical work, present it for review, constructive criticism, and prepare for publication. Based at Summerland with travel or stay at the University of BC – Okanagan nearby, or University of Western Ontario, depending on the project and academic interaction required. Possible interactions with fruitgrowing organizations in Canada, USA, and other international collaborators. Expected qualifications Proficient English. Biologist interested in molecular, analytical, or computer skills, or a mathematical person interested in biology. Interest in one or more of: spatial ecology, landscape genetics of insects, Geographic Information Systems, microclimatic data, modelling of movement and invasion. Benefits to scholar Opportunity to work with an established group of biologists, modeller, statisticians, and a molecular ecologist. To develop skills with molecular, modelling, or GIS-based technology in the important new area of spatial and landscape ecology. Experience working in a GIS project analysing the largest dataset in Canada of physical topography, land use, micro-climates, and spatio-temporal insect counts; working with mark-release-recapture experiments; agent-based modelling; or molecular probes (proteins or microsatellite DNA) of moths or flies. (Update: January 28, 2009) Page 74 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Swift Current_01 Back PROJECT TITLE / TITRE DU PROJET: Identification of molecular markers linked to disease resistance through linakge analysis and association mapping approaches in durum wheat Internship location in Canada Semiarid Prairie Agricultural Research Centre, Internship Duration/Durée du Emplacement du stage Swift Current, Saskatchewan stage: 24 months/mois Contact: Asheesh (Danny) K. Singh Email/Courriel: singhak@agr.gc.ca Phone/Téléphone: 1-306-778-7256 Name of Research Centre/Nom du Centre de Recherche : AAFC-Semiarid Prairie Agricultural Research Centre Mailing address/Adresse postale du Centre : P.O. Box 1030, Swift Current, SK, Canada S9H3X2 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre): Dr. Asheesh K. Singh, Durum Breeder, Sustainable Production Systems Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Ron Knox, Dr. Myriam Fernandez, Dr. Jeannie Gilbert, Dr. Ron DePauw, Dr. Fran Clarke, Dr. T. Fetch, Dr. B. McCallum University collaborators/Collaborateurs universitaires: Dr. Curtis Pozniak Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Project background and rationale: Durum is an important world crop (for area, production, $ value) and diseases are major obstacles to producing high yield and quality crop. Identification of genetic resistance in durum wheat germplasm can impact profitability and sustainability of Canadian durum and stabilize international market because Canadian durum occupies the largest share of the world market. The focus of this research will be stripe, leaf and stem rusts; tan spot; septoria leaf blotch; and common root rot. Objective: The objective of this project will be to identify new sources of resistance to these pathogens, and then utilize existing and new molecular marker information to tag these resistance genes. Outcome expected: Our research groups at Agriculture and Agri-Food Canada and the University of Saskatchewan has genotyped an association mapping population consisting of diverse germplasm from around the world and several bi-parental mapping populations using molecular markers. Evaluation of disease resistance in this diverse set of germplasm will be useful to identify disease resistance genes and linked DNA markers. This research will characterize the complement of disease resistance genes existing in the Canadian germplasm pool. Disease screening will be conducted in the greenhouse and field conditions in multi-location, -replicated trials. The proposed disease evaluation will help identify sources of resistance; and coupled with extensive molecular data already compiled, will permit association genetic studies to identify novel genes. This will be beneficial for breeders to use as a source of disease resistance through conventional breeding and marker assisted breeding. The outcomes of this project will be the identification of new sources of disease resistance to several pathogens that can be used in cultivar development efforts in Canada and worldwide. The subsequent output of new cultivars with disease resistance will be available to producers, thus reducing current inputs for disease control and lowering the impact of chemical use on the environment. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. We have done extensive genotyping with molecular markers of the bi-parental and association mapping populations. There will be opportunities to do further molecular work in the lab. Disease resistance screenings in the field and greenhouse are the planned role of a post-doctoral-fellow (PDF) along with molecular marker analysis. The PDF will also be involved in the statistical analysis, and publication writing. There is flexibility for the PDF to incorporate new ideas in the broader scope of disease resistance breeding. Experience/education in plant genetics and pathology is required. It will be desirable to have background in molecular markers, breeding and statistics. The intern will be provided the opportunity to do QTL and association mapping analysis, and to work as a part of a multidisciplinary team, and will have freedom to contribute new ideas, and write publications from their project. The PDF will have the opportunity to be involved and learn in the wheat breeding program at SPARC-AAFC. (Update: January 28, 2009) Page 75 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Winnipeg_01(Morden) Back PROJECT TITLE / TITRE DU PROJET : Genetic diversity and breeding use of dry bean (Phaseolus vulgaris L.) germplasm Internship location in Canada/ Cereal Research Centre - Morden Research Station, Internship Duration/Durée du Emplacement du stage Morden, Manitoba stage: 12 months/mois Contact: (name of AAFC scientist / nom du chercheur d’AAC) Email/Courriel: houa@agr.gc.ca Phone/Téléphone: 1-204-822-7228 Anfu Hou Name of Research Centre/Nom du Centre de Recherche : AAFC-Cereal Research Center-Morden Research Station Mailing address/Adresse postale du Centre : Unit 100-101 Route 100, Morden, MB, Canada R6M 1Y5 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Anfu Hou, Bean Breeder Research Scientist Other AAFC collaborators/Autre collaborateurs à AAC: Dr. Robert Conner, Research Pathologist University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Sustainable dry bean production relies on steady-flow of cultivars with high yield potential, disease resistance, and marketable seed quality. Public elite genetic materials suitable for hybridization and breeding selections are very limited and becoming restrictive factors in breeding. Genetic diversity exists in world wide dry bean resources, and can be estimated by modern technologies such as DNA markers. Evaluation of genetic diversity and characterization and identification of new genetic resources for adaptation to Manitoba will expand regional gene pool, facilitate molecular marker-assisted cultivar development, and improve efficiency of germplasm conservation. Objective/Objectifs: To evaluate genetic diversity in dry bean germplasm using molecular markers and develop diverse breeding materials for adaptation to Manitoba. Outcome expected/Résultats attendus: 1). Phenotyping of agronomic traits in dry bean genetic materials for use in breeding and gene mapping; 2). Estimation of genetic diversity in worldwide dry bean germplasm collections; 3). Publication of research results in newsletters and international refereed journals. C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The internship program is designed for a postdoctoral researcher to conduct research and gain training in modern crop breeding and genetic practices, including technologies in molecular markers, seed quality analysis, disease resistance screening and gene mapping, genetic diversity evaluation, and genetic enhancement of germplasm resources. The qualified candidates are expected to have advanced knowledge and training in disciplines of plant science, especially in the areas of plant genetics and breeding, plant pathology, and molecular markers and technology. Fluency in English language is required. Willingness to do field-work is a prerequisite. The successful applicant should be able to work in a multi-disciplinary team, and be multi-task oriented. The training will provide opportunities to the candidate for experience in both conventional and molecular plant breeding and genetics, and lead to a successful career in managing a modern comprehensive breeding program. (Update: January 28, 2009) Page 76 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC PROJECT ID/ NO DU PROJET: 2009_Winnipeg_02 Back PROJECT TITLE / TITRE DU PROJET : Novel molecular markers for wheat improvement Internship location in Canada: Cereal Research Centre, Internship Duration/Durée du Emplacement du stage Winnipeg, Manitoba stage: 24 months/mois Contact: (name of AAFC scientist / nom du chercheur Email/Courriel: Mark.Jordan@agr.gc.ca d’AAC) Mark Jordan Phone/Téléphone: 1-204-983-1453 Name of Research Centre/Nom du Centre de Recherche : AAFC-Cereal Research Centre Mailing address/Adresse postale du Centre : 195 Dafoe Rd., Winnipeg, MB, Canada R3T 2M9 Website/site Web: http://www.agr.gc.ca/science A – The Research Team / L’équipe de recherche Supervisor/superviseur: (name and title/ nom et titre) Dr. Mark Jordan, Section Head, Molecular Genetics Other AAFC collaborators/Autre collaborateurs à AAC: Mr. Travis Banks, Dr. Gavin Humphreys University collaborators/Collaborateurs universitaires: Industry partners/Partenaires industriels: B – Project Description: rational, objectives, outcome expected / Description du projet: rationnel, objectifs, résultats attendus Background, Rational, Issue / Contexte, Rationnel, Problématique: Our group are leaders in the development and application of genetical genomics tools to wheat. We were the first group to supply proof of concept that whole genome expression quantitative trait loci (eQTL) analysis (Jordan et al., 2007) and single feature polymorphism (SFP) analysis and mapping (Banks et al., 2008) are feasible in wheat. We found that several genome regions contained hotspots which explained a significant amount of the variation in expression of many genes. These regions could harbour regulatory genes important in seed development and end-use quality. What is needed is a larger population size to validate these regions. In addition, more markers can be applied to the map using the same data used for eQTL analysis. This relies on taking advantage of the design of the microarray chips used to identify and map SFPs. Using a single time point in seed development we have identified and mapped over 1000 SFPs. The potential now exists to combine eQTL and SFP analysis in a larger population over several time points in seed development. This will provide a resource that will include the addition of thousands more potentially useful molecular markers to wheat maps, provide the physical location of thousands of genes expressed during the course of seed development and identify genome regions responsible for regulating seed expressed genes. These can then be associated with trait QTL data (agronomic and extensive end-use quality traits) already collected on this population. Banks, T.W. et al (2008). In The 11th International Wheat Genetics Symposium proceedings. http://hdl.handle.net/2123/3375 Jordan, M.C. et al. (2007). Plant Biotechnol. J. 5:442-453. Objective/Objectifs: Identify SFPs in several time points of seed development in the RL4452 X AC Domain population Map the SFPs along with existing SSR data for the population Validate the SFPs using robustness, rice synteny, PCR and 454 sequencing Create a database of mapped sequenced based polymorphisms which will identify the physical location of genes expressed in seed development and relate these to existing eQTL data to assist in the identification of regulatory genes Outcome expected/Résultats attendus: By assigning more markers to the linkage map the database will assist map based cloning efforts in wheat. Ultimately this will allow the identification of key regulatory genes controlling seed development and end-use quality and provide molecular markers for these genes that can be used in molecular breeding efforts to improve end-use quality (food or industrial uses derived from grain). C – Internship: Describe the internship program, qualifications of and the benefits to the expected intern / Stage: Décrire le stage, les qualifications demandées et les avantages pour l’interne. The intern will be involved in aspects of QTL and genetic linkage mapping using traditional and novel molecular markers. This will include integrating new molecular marker data (eg. SFP) with currently available data using existing segregating populations, association populations, QTL data, gene expression and marker data. The intern will gain experience in aspects of wheat genomics including associating genotype to phenotype, identifying novel molecular markers, associating markers to (Update: January 28, 2009) Page 77 of 78 2009 Postdoctoral Internship at AAFC / Stage postdoctoral 2009 à AAC traits, new sequencing technology and microarray analysis. This experience will allow the development of expertise in modern methods in molecular breeding that can be applied to the wheat breeding programs in the intern’s home country. The intern must have a strong background in statistical genetics including QTL analysis, creation of genetic linkage maps and knowledge of associated software and theory. Experience in association mapping and aspects of bioinformatics such as sequence assembly would be beneficial. The intern will collaborate with AAFC scientists involved in wheat breeding, bioinformatics, genetics and genomics and be involved in projects to identify genes, genomic regions and molecular markers involved in traits such as improved human nutrition, pre-harvest sprouting and insect resistance. (Update: January 28, 2009) Page 78 of 78