Basic OB Prenatal Diagnostic Tests Chapter 16 lecture Ultrasound Transvaginal Done during the first trimester to determine pregnancy, confirm fetal viability, estimate gestational age, determine number of fetuses, and in conjunction with other tests. Transabdominal Done during the second and third trimesters to assess above components plus to evaluate fetal anatomy, assess progress of fetal growth, evaluate amniotic fluid volume, and determine presentation and location of cord and placenta. Advantage is that it is noninvasive and disadvantages are that it is expensive and cannot detect all fetal anomalies. Alpha-fetoprotein screening Measures AFP in maternal serum and in amniotic fluid. See box 162 for conditions associated with abnormal levels. Evaluated between 16 and 18 weeks usually through maternal serum. Advantages include minimal invasiveness and is inexpensive. Limitations include that it is a screening test and abnormal results must be followed by more tests. It is also very time sensitive so screening outside of the 16-18 week window offers inaccurate results and benign conditions can cause abnormal readings. Triple-marker screening In addition to MSAFP, hCG and unconjugated estriol are added to screen for chromosomal abnormalities. Chorionic Villus Sampling Samples of chorionic villus cells can be used for diagnosis of fetal chromosomal, metabolic, or DNA abnormalities between 10 and 12 weeks gestation. Indicated only for those at high risk for genetic anomalies. Procedure CVS can be performed by a transcervical (catheter inserted through the cervix) or a transabdominal (needle inserted through the abdomen) approach. The advantage is that this is performed early in the pregnancy, allowing for a first trimester abortion if the results are abnormal (for those who would consider an abortion as an option). The disadvantages are a higher pregnancy loss rate than with amniocentesis, a higher limb reduction rate in CVS performed before 10 weeks, and the possibility of Rh sensitization. Amniocentesis Aspiration of amniotic fluid and can be performed in the 2nd or 3rd trimesters. See Box 16-3 for common indications. Procedure Using US to locate the fetus and placenta, a needle is inserted into the largest pocket of fluid and fluid is withdrawn for analysis. The mother will then be monitored for UC and FHT for the next ½ to 1 hours. Advantages include low fetal loss rate while permitting diagnosis of many anomalies. Also used to determine fetal lung maturity. Disadvantages include later diagnosis than with some other methods and slight risk of spontaneous abortion. Nonstress test (NST) This assesses fetal heart tones for accelerations, especially those associated with fetal movement. Accelerations are associated with adequate oxygenation and a healthy nervous system. Procedure The woman is monitored for UC and FHT. She may also be instructed to “mark” any fetal movement. A reactive strip is one that occurs during a 20 minute period in which the fetal heart rate goes above baseline twice by at least 15 bpm and for at least 15 seconds. Nonreactive strips do not meet this criteria…see figure 16-8. Advantages include noninvasiveness and may be repeated daily. A disadvantage is a false positive due to fetal sleep but this can be corrected by prolonging the test for at least 40 minutes. Contraction Stress test If the fetal oxygenation appears marginal during the NST, contractions may be added to measure the response to stress. Late decelerations, or those where the fetal heart rate decreases after the contraction peaks and doesn’t return to baseline until after the contraction has ended, suggest inadequate oxygen reserves, Procedure Either IV pitocin or nipple stimulation is used to produce at least three contractions of 40 seconds duration within a 40 minute period. The fetal heart rate pattern is then interpreted. Advantages are few, disadvantages include hyperstimulation of the uterus, time required, and expense. Biophysical Profile This test assesses five parameters of fetal well being: NST, fetal breathing movements, gross fetal movements, fetal tone, and amniotic fluid volume. See table 16-1 for interpretation. This is a frequently used test due to low cost and high sensitivity. Percutaneous umbilical blood sampling Aspiration of fetal blood from the umbilical cord. Disadvantages include fetal loss and premature labor. Maternal Assessment of Fetal Movement (Kick counts) This is the easiest method to evaluate fetal wellbeing. Like an infant, the fetus has sleep/wake cycles and the mother is usually aware of these. The mother is instructed to count kicks or fetal movements during a time that the fetus is normally active. The duration is usually for an hour and the mother can make comparisons from day to day. Obviously a significant decrease in kicks should be reported to the healthcare provider along with a significant increase (an increase precedes a decrease in some situations). Giving Birth Chapter 17 lecture S/S of labor Lightening Increased level of activity Braxton Hicks Cx Cervical ripening UC Show ROM Components of labor spines Fetal presentation Engagement Widest part of fetus is at the level of the ischial spines “0” station Station Relationship of the presenting part to the ischial Ranges from -2 to +2 +3 to +4 is on the perineum Fetal lie whether the fetus is in a horizontal or vertical position cephalic presentation breech shoulder Fetal positions pgs 348, 349, & 350 Powers of labor UC Increment, acme, and decrement Contour changes…Bandl’s ring Pushing Stages of labor First stage (begins with true labor and ends with full dilation/effacement) Latent (up to 3cm) Active (4-7cm) Transition (8-10cm) Second stage (complete-birth) Third stage (birth-expulsion of placenta) Placental separation Placental expulsion Fourth stage (recovery, 1-4 hours) Assessment in labor Early Leopold’s maneuvers ROM If membranes spontaneously ruptured prior to admission, the time is determined. Whether artificially ruptured or spontaneously, the color and quality of the fluid is assessed. Clear is good and may have bits of a creamy white substance (vernix). Fluid should have no odor. Yellow fluid and foul odor suggest infection. Green fluid with or without chunks suggest fetal distress. ROM also ends the mother’s ability to get up and walk unless the presenting part is well-engaged. SVE VS Labs UC Length, intensity, frequency Effacement Dilatation External vs. internal monitoring Care of the woman during the first stage of labor Keep bladder empty Change linens, gowns frequently Pain mgmt Support people Care of the woman during the second stage of labor Laboring down Med mgmt Delivery table setup/prep Positioning Pain mgmt Pushing Episiotomy Birth Cutting and clamping the cord Care of the woman during the third and fourth stages of labor Placenta delivery Perineal repair Oxytocin Recovery assessment Newborn care in delivery room After cord is clamped, if baby looks good, mother may hold momentarily, then baby is taken to radiant warmer, dried off quickly and towel discarded, hat applied, bracelets applied to wrist, foot, and mother’s arm. Footprints taken along with mother’s thumb print…all before mother and baby are separated. Baby suctioned and possibly deep suctioned if not establishing respirations. Newborn assessment in the delivery room Apgar Pg. 385 Done at 1 and 5 minutes Not used to determine need for resuscitation, NALS for that Vital signs TPR, bp not routine unless cardiac abnormality suspected Cord is assessed for vessels Voids and stool assessed If baby is doing well, may stay up to one hour with mother before going to nursery but temp evaluated often