Exam 1 – Quarter 2 Review Sheet AP Biology USE THIS SHEET AFTER YOU HAVE STUDIED AS A METHOD OF EVALUATING WHAT YOU STILL NEED TO WORK ON. Exam 1 will cover: ALL of Chapter 6 and extra PowerPoint info (STOP HERE…have you studied yet? If yes, continue… If no, GO STUDY FIRST! DO NOT USE THIS TO STUDY!) 1. Study the Prokaryote vs Eukaryote Chart under notes section 2. Study the Organelle Chart under notes section 3. What is the name of the artist who painted the painting shown in the PowerPoint? What do art historians believe this is a painting of? Explain. 4. Describe the significance of the Dutch microscopist Anton van Leeuwenhoek (16321723) and the English Robert Hooke (1635 – 1703). Describe cell theory and identify the three scientists accredited with this theory and their contributions. 5. Explain why hydrophilic molecules like proteins, amino acids, carbohydrates, nucleic acids, Na+, other salts, etc… are NOT able to move through a plasma membrane, while small hydrophobic molecules can. Why do you think large hydrophobic molecules have trouble crossing? 6. What is a hormone? Give an example and include the origin of the hormone, the target organ, and the affect on the body. Why does this hormone not target any other cells when it is all over the body? 7. Explain why amino acid/polypeptide/protein hormones require a cell surface receptor (embedded in the membrane) protein in order to send a signal to the cell (talk to the cell), while steroid hormones typically have protein receptors inside the cell, soluble in the cytoplasm? 8. Compare and contrast the three different types of microscopes we learned about. How are they similar? How are they different? What are the advantages and disadvantages? How are samples prepared for each? Magnifications? Resolutions? Know when to use each if you were working in a lab. 9. Identify and describe the different types of light microscopes available. Explain how the fluorescent microscope works – give a real like example. 10. What is the definition of resolution? What is a better resolution, 5um or 120nm? Explain why. 10.5 Be able to calculate the magnification of a light microscope knowing the ocular and objective magnifications. 11. You should be able to calculate either the FOV under high power, FOV under low power, high power magnification, or low power magnification when you know three of the four variables. Sample questions are in the PowerPoint. 12. Explain what happens to the size of the FOV under high power as compared to low power. Why does this happen? Why can one not use a ruler under high power to measure the FOV? 13. Explain the orientation of an object as viewed through a microscope as compared to its orientation on the slide itself. Check out the virtual microscope under the misc section on the lab page if you don’t recall what happens to the letter “e”. 14. Describe how to prepare a wet mount. 15. Describe how you would estimate the size of an object under low power if you know the FOV diameter. 16. Explain how to focus a light microscope under high power beginning with placing the slide on the microscope. 17. How many microns in a millimeter? How many nanometers in a micron? How many nanometers in a millimeter? Be able to convert. Draw a ruler indicating a meter as we did in class and show the definitions of mm, um and nm using the picture by breaking the distances up into a 1000 equal lengths each time. 18. Give a structure-function example in terms of cells. 19. Explain why cells are limited in how big a cell can be. Be sure to discuss the surface area to volume ratio. Use an example to show your reasoning. 20. What limits how small a cell can be? 21. Be able to label prokaryotic and eukaryotic cells. 22. Compare and contrast prokaryotic to eukaryotic cells. Be able to explain the function and location of every structure. 23. Explain how a protein, made in the cytoplasm, can gain entrance to the nucleus. Give an example of a protein that needs to gain entrance to the nucleus. 24. Download and master the eukaryotic organelle chart. “Master” means to be able to reproduce the entire chart without looking at it and be able to teach another person about each organelle in a conversation. When I say eight time eight, you say 64. When I say smooth endoplasmic reticulum, you say lipid synthesis, detox, Ca++ storage in muscle. It should be automatic. 25. Draw a single membrane like in lysosomes and a double membrane like the nucleus has showing how they are different. What other organelles have a double membrane? How do we theorize this occurred? 26. Explain how 6ft of DNA is packed into a tiny nucleus at a diameter of 1/1000th of a mm. 27. What is a ribosome made of? Where is it made? What genes (segments of DNA) would you hypothesize to find located at the nucleolus? 28. How does the cytosol and cytoplasm differ? 29. Compare and contrast chromatin, chromosome and DNA? 30. How many chromosomes (books) are there in a human nucleus? Are all of these books completely different/unique? Explain. Where did your chromosomes come from? 31. Explain why I call cytoskeletal elements an example of extreme quaternary structure. 32. Make a chart that details the organelles present only in animal cells vs. those that are present only in plant cells. 33. Explain in great detail, starting with the chromosomes in the nucleus, how a secretory protein like insulin is made and transported out of the cell. Explain what a “secretory protein” is... 34. Explain how new membrane (phospholipids) are added to the cell membrane when a cell is growing in size or needs to replace phospholipids that have broken down. 35. Explain how a transport vesicle is sent from the RER to the golgi. 36. A cell needs to make a few new lysosomes. Explain how it goes about doing this. 37. THIS IS A QUESTION: You are inside a liver cell taking a cytoplasmic swim. On the outside, you observe insulin molecules bind insulin membrane receptors. This causes the genes for the glucose transporter, an integral membrane protein that allows glucose to enter cell, to be turned on so that the liver cell can take up the excess extracellular glucose. Starting from the gene, explain how glucose transporter proteins will find their way to the plasma membrane so that they can do their job. Make sure you include the following terms: RNA polymerase, nucleus, chromatin, chromosome, DNA, nuclear pore, N-terminal signal sequence, SRP, SRP receptor, Translocon, ribosome, small ribosomal subunit, large ribosomal subunit, translocate, dehydration synthesis, codon, anticodon, A-site, Psite, E-site, stop codon, release factor, 5’ to 3’, cap, poly-A tail, AUG, peptide bond formation, mRNA, tRNA, rRNA, amino acids, ER, Golgi, transport vesicle, secretory vesicle, fusion, cis-maturation model, cis, trans, rough ER, randomly, thread, pinch, microtubule, kinesin, ATP, glycosylation (two sites), ER resident enzymes, golgi resident enzymes, transcription, translation. 38. The golgi has two different sides. A receiving or CIS cisterna and a shipping or TRANS cisterna. Explain how proteins arriving in vesicles to the golgi make their way from Cis to Trans. You should watch the protein trafficking video (cisternal maturation model). 39. You should be able to describe what is happening in every figure in chapter 6 as well as be able to label them without exception. Cover them with a piece of paper and describe them to yourself, you friend, a stuffed animal…I don’t care. Be able to label and describe. 40. Explain the two lysosomal storage diseases and come up with a hypothetical method of treating or curing these conditions. Watch the Tay-Sachs movie under misc section of website – chapter 6. Why are they called storage diseases? 41. Be able to describe how a protein is synthesized. Watch the protein synthesis animation under misc on website – virtual cell animations. Remember that the tRNA brings the amino acid and base pairs with the mRNA and the ribosome catalyzes the formation of the peptide bond (dehydration synthesis) etc… Watch the video over and over and over and over and over until you get it. 42. Explain the premise/evidence behind the endosymbiotic theory. 43. Compare and contrast both the structures and functions of chloroplasts and mitochondria. How, when working together, do these two organelles illustrate energy flowing through an ecosystem and matter/nutrients cycling through it? 44. You should be able to draw both a simple chloroplast and mitochondria and be able to label your drawings. 45. Explain the structure and function of flagella/cilia. 46. Explain how flagella/cilia move. 47. What is meant by an MTOC? 48. How are the centrosomes from plants different from those of animals. 49. Compare and contrast the structures of centrioles, basal bodies, and the core microtubule structure of flagella and cilia. 50. Compare and contrast the three different fibers of the cytoskeleton in terms of structure and function. 51. Describe the structure and function of the three types of cell junctions and give real life examples of where they are found. 52. THIS IS A QUESTION(S): You must watch the inner life of the cell video and know exactly what you are observing throughout the entire video. There will be video/still shot questions pertaining to this animation. It can be found under the misc section of the website. 53. Explain what is meant by the cytoskeleton is “dynamic”. 54. Make sure you know the central dogma. 55. Compare endergonic to exergonic. 56. Define and give lots of examples of energy coupling. 55. THIS IS A QUESTION: Water to water question. You are an electron in a water molecule in the soil. Describe your travels from this water molecule to another as you pass through photosynthesis and cellular respiration within a plant. End in a water molecule in the atmosphere. Draw out the overall reactions of photosynthesis and cell resp and use these as figures to refer to when you write your text. The following terms must be included and in proper context within your answer. If I cannot tell that you know what the word means then I cannot give you credit. Oxidize, reduce, cellular respiration, photosynthesis, chloroplast, thylakoid disc, grana, thylakoid membrane, stroma, mitochondria, matrix, inner membrane, cytosol, glycolysis, Krebs, ETC, Grooming, Light reactions, Dark reactions, Calvin cycle, electronegativity, light, kinetic energy, potential energy, affinity, ADP, P, ATP, exergonic, endergonic, energy coupling 56. Discuss the importance of peroxisomes and include the reactions occurring within them. Why do these reactions need a special compartment? 57. Describe the different types of plastids and how they arise. 58. That is all I can do…the rest is for you. STUDY WELL