Modupe Felicia Adesina 1611 Huron street • London, Ontario N5V 3A4 • 226-777-5351 • 519-200-8744 m.adesina@gmail.com SUMMARY OF QUALIFICATIONS Over 7 years research experience in the field of agronomy, soil microbial ecology, plant pathology, bacteriology, plant-microbe interaction and biological disease control Experience in microbiological techniques: all basic microbiological techniques and biochemical testing Experience in study of microorganisms and their disease causing activities (e.g. Rhizotonia solani, Fusarium oxysporum, Ralstonia solanacearum and Streptomyces scabies) Experience in working with food crops (lettuce, potato and corn) and soil samples Experience in technical writing, experimental design, results analysis and dissemination at scientific meetings Experience in post-secondary school teaching and managing students through provision of guidance to strengthen their skills Possess excellent administrative skills Possess strong multi-tasking and interpersonal skills with relationship management Excellent English proficiency, with strong written and oral communication skills Proficiency in the use of Microsoft Word, Excel, Adobe Photoshop and PowerPoint presentation TECHNICAL SKILLS Plant Health and Field Work Soil and plant samples handling and storage Growth-chamber, greenhouse and field work with various cropping systems Insecticides, herbicides and Fertilizer application Crop harvest and storage Dual culture screening of bacteria for activity against plant pathogens Plant inoculation with beneficial bacteria Knowledge in mode of action of biological control agents, their interaction with plant host and environment; and use as inoculants Microbiology Bacterial and fungal isolation on general and specific media from different environmental samples by dilution techniques Microbial cultivation, enumeration, storage and maintenance Preparation of general and selective media as well as laboratory reagents Bacterial staining and biochemical analysis Sterilization of laboratory equipment and tools In vitro screening of bacterial isolates for antagonistic activity against plant pathogens by dual culture plate assay and enzymatic activities Inoculation of plant and soil with microbial culture Isolation, extraction and purification of secondary metabolites from bacterial culture for High Performance Liquid Chromatography (HPLC) analysis Microscopic observation of microbes Page 1 of 6 Molecular Techniques DNA (plasmid and genomic) and RNA extraction from bacteria and environmental samples DNA and RNA quantification on agarose gel and with spectrophotometer Bacterial transformation and preparation of competent cells PCR analysis – conventional PCR, Reverse transcriptase PCR, and Quantitative PCR; RFLP and ARDRA Design of PCR primers and Taqman-probes Denaturing Gradient Gel Electrophoresis (DGGE) for analysis of microbial diversity and structure in environmental samples Southern blotting and probe formation Gene cloning Teaching Effective classroom teaching techniques Course outlines/curricula and materials development Students’ performance assessment Computer Data analysis using Excel and SAS Microsoft and Power-point usage EXPERIENCE Part-time Laboratory Instructor (Microbiology) Fanshawe College, London, Ontario Taught introductory microbiology course and provided practical microbiology knowledge to students Actively involved in laboratory teaching, discussion and ensured attendance and participation of students in the laboratory Designed and organized course/laboratory materials Ensured compliance with laboratory safety procedures and enforced a high standard of behavior and discipline among students Assessed and graded students’ laboratory reports and tests Lecturer/Farm Supervisor All Nations University College (ANUC), Koforidua, Ghana Jan 2015 – April 2015 Jan., 2014 – Dec. 2014 Taught microbiology and molecular biology courses to undergraduate students Actively involved in classroom teaching, discussion, debate and ensure attendance and participation of students in class Mentored and supported students at both academic and personal levels Assessed students course work and materials Invigilated examinations and marked scripts in a timely fashion Course outlines and materials design with organization of industrial attachment to enhance hand-on practical experience of students Enforcement of high standard of behavior and discipline among the students and faculty members Page 2 of 6 Carry out administrative roles as the assistant head of the department : organize and conduct departmental staff meetings, review departmental requests and staff appraisals and ensure collaborative atmosphere within the department Conduct interviews and orientations for new staff members and ensure professional growth among members Managed and supervised activities at the university crop, animal and forestry units Post-Doctoral Research Fellow (NSERC) Agriculture Agri-Food Canada (SCPFRC), London, Ontario Participated in three funded projects (by OMAFRA and Horticultural Australia limited) on improving plant growth, nutrition and crop yield through use of beneficial endophytic and rhizosphere bacteria Investigated the effects of crop rotation and green-manure (pearl-millet) incorporation into soil on soil fertility, potato yield, Verticillium wilt and common scab disease Isolated free-living nitrogen fixing and antibiotic producing bacteria from root and endophytic tissue of corn and potato plants Characterized bacterial and fungal isolates based on in-vitro antibiotic and enzymatic activities, such as protease, glucanase, chitinase, 2,4-Diacetylphloroglucinol, pyrrolnitrin and phenazine productions Screened antibiotic producing bacteria against plant pathogens from large environmental bacterial strains Carried out greenhouse and field assessment of nitrogen fixing and antibiotic producing bacteria on promotion of growth and yield of corn and potato Assessed environmental impact of biocontrol inoculants introduced into the soil using molecular fingerprinting technique (PCR based Denaturing Gradient Gel electrophoresis) Provided effective leadership, management, organization and supervision of daily experimental work to undergraduate students according to research objectives and ensured projects timelines are met Ensured team members complied with field and laboratory safety and procedures Prepared detail writings of experimental protocols and results Presented research results and recommendations at scientific meetings or conferences Volunteer Visiting Scientist University of Manchester, Faculty of Life Sciences, England 2007 – 2008 Conducted research: “Cultivation-independent analysis of sulphatase-producing bacteria in the rhizosphere” Extracted plasmid DNA and prepared competent bacterial cells Transformed and tagged bacteria with plasmid carrying gene(s) of interest and screened transformedbacterial cells for gene(s) of interest Prepared reagents, materials and equipment used in microbiological testing in sterile environments Doctoral Fellow Federal Biological Research Center for Agriculture & Forestry, Braunschweig, Germany 2008 – 2011 2002 – 2007 Completed a project (denoted METACONTROL) aimed at elucidating consortia of beneficial bacteria residing in suppressive and non-suppressive soils of Europe (Germany, Sweden, the United Kingdom, France and the Netherlands) Assessed bacterial isolates for antagonistic potential against soil-borne plant pathoges (Pythium ultimum, Fusarium oxysporum, Rhizoctonia solani and Ralstonia solanacearum) via in-vitro and in-vivo assays Assessed the diversity of antifungal producing bacteria in rhizobacteria using molecular biology techniques Carried out series of greenhouse experiments to evaluate effects of potential bacterial antagonists on control of bottom rot disease caused by R. solani AG1-IB on lettuce Page 3 of 6 Isolated bacterium that could be considered as strong biocontrol agent and used for suppression of this pathogen Assessed the environmental impacts of biocontrol agents applied as seed inoculants on indigenous microbial population using molecular fingerprinting technique (PCR-DGGE analysis) Research Fellow International Institute of Tropical Agriculture (IITA), Nigeria 1997 – 1999 Conducted greenhouse experiment to determine symbiotic interaction between mycorrhiza and Rhizobium Analyzed available phosphorus, mineral nitrogen from plant and soil samples Determined mycorrhiza infection rate on the roots of cowpea plants EDUCATION Ph.D in Biology (Microbial Ecology) Technical University, Carolo-Wilhelmina, Braunschweig, Germany 2007 Evaluated by World Education Services as equivalent to earned doctorate (Ph.D.) Degree from a Canadian University Master of Science in Agronomy (Soil Science) University of Ibadan, Ibadan, Nigeria 2000 Evaluated by World Education Services as equivalent to a two-year Master’s of Science Degree from a Canadian University Bachelor of Science in Soil Science University of Ibadan, Ibadan, Nigeria 1997 Evaluated by World Education Services as equivalent to a four-year Bachelor’s Degree from a Canadian University SCHOLARSHIPS/GRANTS RECEIVED NSERC-Visiting Fellowship May 2008 – Apr 2011 Deutscher Academische Ausland Dienst (DAAD) Scholarship to pursue a doctorate degree in Germany Apr 2002 – Jun 2006 Page 4 of 6 PUBLICATIONS IN PEER-REVIEWED JOURNALS Adesina, M.F., Grosch, R., Lembke, A., Vatchev, T.D., Smalla, K. (2009). In vitro antagonists of Rhizoctonia solani tested on lettuce: rhizosphere competence, biocontrol efficiency and rhizosphere microbial community response. FEMS Microbiology Ecology 69: 62-74 Hjort, K., Bergström , M., Adesina, M.F., Jansson, J.K., Smalla, K., Sjöling, S. (2009). Chitinase genes revealed and compared in bacterial isolates, DNA extracts and a metagenomic library from a phytopathogen-suppressive soil. FEMS Microbiology Ecology 71: 197-207 Adesina, M.F., Lembke, A., Costa, R., Speksnijder, A., Smalla, K. (2007). Screening of bacterial isolates from various European soils for in vitro antagonistic activity towards Rhizoctonia solani and Fusarium oxysporum: site-dependent composition and diversity revealed. Soil Biology and Biochemistry 39: 28182828 TALKS AND POSTERS PRESENTED AT CONFERENCES/SYMPOSIA Adesina, M.F. , Ramarathnam, R., Reynolds, B., Lazarovits, G. “Effect of pearl millet-green manure applications on potato yield and disease suppression” Presented at the 13th International Symposium on Microbial Ecology. Book of abstracts, Seattle, WA, USA, August 22 – 27, 2010 Adesina, M.F. “Assessment of antagonistic bacteria from suppressive soils for biological control of Rhizoctonia solani AG1-IB on lettuce” Presented at the “Tag der Junge Wissenschaft 2006’ in the Institute for Vegetables and Ornamental crops,IGZ, Großbeeren/Erfurt e.V, Germany, August 30, 2006 Adesina, M.F., Grosch, R., Lembke, A., Smalla, K. “Assessment of antagonistic bacteria from suppressive soils for biological control of Rhizoctonia solani AG1-IB on lettuce” In: 11th International Symposium on Microbial Ecology ISME-11. Book of Abstracts, Wien, Austria, August 20 – 25, 2006 Hjort, K., Bergström, M., Sjöling, S., Adesina, M. F., Smalla, K., Jansson, J. K. “Metagenomic analysis of antifungal activity in a suppressive soil” In: 11th International Symposium on Microbial Ecology ISME-11. Book of Abstracts, Wien, Austria, August 20 – 25, 2006 Lembke, A., Ding, G.C., Adesina, M.F., Smalla, K. “Diversity of Streptomycetes specific chitinase genes in suppressive and non-suppressive soils” In: 11th International Symposium on Microbial Ecology ISME-11. Book of Abstracts, Wien, Austria, August 20 – 25, 2006 Adesina, M. F., Grosch, R., Lembke, A., Smalla, K.: “Biological control of Rhizoctonia solani AG1-IB on Lettuce plants by two Pseudomonas strains isolated from suppressive soils”. Presentation at the Annual Conference of the General and Applied Microbiology (VAAM). Jena, March 19 – 22, 2006 Lembke, A., Adesina, M. F., Smalla, K. “Comparison of microbial community structure and function of suppressive and non-suppressive soils” presented at 2. Gemeinsamer Kongress der DGHM und VAAM_2005, September 25 – 28, 2005, Göttingen, Germany Lembke, A., Adesina, M. F., Smalla, K. “Microbial community structure and function of suppressive soils compared to non-suppressive soils”. Presentation at BAGECO-2005, 8th Symposium on Bacterial Genetics and Ecology, June 26 – 29, 2005, Lyon, France Page 5 of 6 Adesina, M. F., Lembke, A., Smalla, K. “Isolation and characterization of potential antagonists from suppressive soils” presented at IOBC_2005 “Multitrophic interactions in soil”, Wageningen, The Netherlands, June 5 – 7, 2005 Lembke, A., Adesina, M. F., Smalla, K. “Chitinase producing bacteria – a reason for suppressiveness in soils?” presented at IOBC_2005 “Multitrophic interactions in soil”; Wageningen, June 5 – 7, 2005 Lembke, A., Adesina, M. F., Smalla, K. “Characterization of bacterial communities in suppressive soils” In: 10th International Symposium on Microbial Ecology ISME-10. Book of Abstracts, Cancun, Mexico, August 22 – 27, 2004, 258 Lembke, A., Adesina, M. F., Smalla, K. “Charakterisierung von Bakterien-Gemeinschaften in supressiven Böden“. 54th Deutche Pflanzenschutztagung, Sept. 20 – 23, 2004, University of Hamburg Adesina, M. F., Lembke, A., Smalla, K. “Characterization of Bacterial community in Suppressive soil” presented at Eurosoil Conference 2004, September 4 – 12, Freiburg, Germany Komolafe, M. F., Lembke, A., Smalla, K. “Characterization of Bacterial community in Suppressive soil” presented at Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM) 2004, Technical University Carolo-Wilhelmina Braunschweig, Germany, March 28 – 31, , 2004 Komolafe, M. F., Lembke, A., Smalla, K. “Characterization of bacterial community in suppressive soils” Presentation at International symposium of structure and function of soil microbiota, Philipps-University Marburg, Germany, September 18 – 20, 2003 REFERENCES AVAILABLE UPON REQUEST Page 6 of 6