Name_____________________________
Cell Reproduction and Genetics Guided Review
1. Go to my website and open the Unit 4 Cell Communication and Reproduction webpage. Click
on the Key to Genetics Practice Problems. Go through the packet. Which types of problems
are hardest for you to remember how to do?
2. For those problems, study the examples thoroughly, then write a couple of sample questions
yourself here:
Next, find your genetic disease table, look at the key, or go to that section in your review
manual. Pick 3 genetic diseases to memorize and write them here – one should be sickle cell
anemia. Include their cause (chromosomal abnormality or nondisjunction or mutation),
symptoms, and if it is dominant or recessive.
3. Next, click on AP Lab 7 Mitosis and Meiosis and read through pages S83-S96.
 In part 1 and part 4, we used pop-beads to model mitosis and meiosis. What are the
main differences between mitosis and meiosis?

Summarize the procedure for part 2, thinking about what we recently did with the onion
bulbs. Remember we used IAA (auxin) instead of lectin. Look back at your onion
mitosis lab to review the chi-square calculation we did.

Summarize the activity for part 3 – karyotypes. How many chromosomes are there and
how are they lined up in a karyotype?
What is the HeLa karyotype and what disease is it associated with?
What is the Philadelphia chromosome and what disease is it associated with?
Name_____________________________

Summarize the procedure for part 5.
What does a crossover ascus look like compared to a non-crossover ascus?
If 40% of the asci show crossover, what is the true crossover frequency? ________
How many map units is the gene from the centromere? ______________
If you were doing crossover frequency to map gene distances in Drosophila, and 2
genes had a 32% crossover, how many map units apart would they be?___________
4. Next, go back and click on AP Lab Genetics of Organisms. This was the virtual fruit fly lab.
Find your actual lab, read through it and summarize the procedure here:

What were the 3 possible scenarios of the different vials?

For the dihybrid cross, if there are 4 possible out comes, you would have 4 sums to
calculate to find the Chi square value. How many degrees of freedom would there be? ___
What would the critical value for p=0.05 be? ___________
5. Next, go back and open the Unit 5 Molecular Genetics and Biotechnology webpage. Open the
guided notes and read through these. Be sure you memorize the big picture. Summarize the
location, key steps, and key enzymes for the following processes:
 DNA Replication =

Transcription =

Translation =
6. Next, go back and click on the Student Transformation Packet for the pGLO lab. Try to find
your original packet and read through it. Summarize the procedure for the lab:

What uses would this procedure have in the real world (what else might scientists do with
this technology)?
Name_____________________________

How is it possible that a gene from an animal can be inserted into a bacterium – what must
they have in common?

Look at how to calculate transformation efficiency. Try to do the calculations on p. 52
again without looking at the answers. Show your work here:
8) Now, go back and click on the DNA Fingerprinting Student Packet. This was the CSI lab. Try
to find your original packet and read through all of it. Summarize the 2 main procedures:

What are the uses of this type of biotechnology in real world practices, besides crime scene
investigation? (You may have to look back at my weekly powerpoint for this)

After making the semilog graph using the known lambda marker DNA, what were you then
able to figure out?
9) Finally, go back and click on the Plasmid Mapping Extension Activity. Find this packet and
read through all of it. Work through questions 7-12 using the S3 data on p. 53 to construct a
plasmid map.