國立台灣大學生物資源暨農學院
農業化學系 九十年年報
（2001 Annual Report of the Department of
Agricultural Chemistry ）
第一項
系所概況
(一) 系所簡介及未來發展重點
本系為台北帝國大學於 1928 年創校時最早設立的學系之一。前身為理農學部農
藝化學科。1945 年台灣光復後，本校改制為國立台灣大學，本系以原日治時代農藝
化學科為基礎，合併農學院其他科系部分講座，更名為農學院農業化學系。由於師
資陣容堅強，設備完善，旋即於 1947 年成立研究所碩士班；1961 年大學部分兩組：
農產製造組與土壤肥料組；1968 年更成立博士班，是國立臺灣大學農學院歷史最悠
久，體制最完備的科系。1994 年研究所增為五組：土壤環境與植物營養組、生物工
業化學組、微生物學組、營養科學組和生物化學組，本系歷史悠久，成立至今已有
七十年，在全體師生的共同耕耘下，我們有輝煌的歷史和燦爛的前程。
本系教學重點著重生物與化學相關科學基礎教育，配合現代科學教育理念及國
際學術研究發展趨勢，強化實驗課程功能，進行實作與研習，並輔以專題討論與專
題研究的學習，使本系大學部畢業生具備實驗室研究經驗。研究所的學習則依循學
生研究主題特別需求，修習不同領域的課程。研究生需參與專題討論課程，於師生
共同參與討論的環境中激發創造力。本所鼓勵學生發表論文於國際科學期刊，並參
與各項學術會議及研討會活動，致力培育具有解決問題能力、獨立進行科學研究的
專業人才為目標。
現代的農業化學，則綜合了化學、物理化學及生命科學的知識與技術，擴大應
用於農業生產環境的保護與控制以及生物資源的高度利用。例如：農業生產對土壤
及環境衝擊的研究與管制，農藥與肥料於環境中之動向監測、污染的防治、環境及
食品微生物之監測、生物技術應用於高價值醫療用品及食品材料的產製研發，農業
與食品相關之植物與微生物之分子生物學研究，食品成份與營養保健相關研究等。
本系（所）目前除擁有一棟三層樓的農化館及一棟五層樓之新建系館外，尚有
農藝館一樓的應用微生物研究室、電子顯微鏡館、機械工廠、土壤環境化學研究室、
土壤調查研究室及玻璃溫室等。
— 1 —
本系今後持續在生物技術和環境科學方面繼續研究發展，教學與研究並重，理
論與應用兼顧。結合社區資源有效利用，提昇國內農業和產業之水準，並提供農業
及產業之技術服務。
(二) 2001 年活動剪影

9 月 13 日本系舉辦 90 年度大一新生入學指導，共有 55 位新生參加。

10 月 22 日本系 1956 級畢業系友返母系參訪，並拜會陳維昭校長，在系務會議
室與本系 20 餘位教師舉行系友座談會，會中系友代表並致贈感謝狀給本系，成
為美好的回憶。

10 月 27 日上午大陸地區中國土壤學會交流訪問團於中華土壤肥料學會辦理「第
四屆海峽兩岸土壤肥料學術交流研討會」，會議期間至本系參訪。

11 月 25 日校慶募款餐會共募到 26 萬元。

12 月 27 日斯洛伐克國家科學委員會院長 Drsc. Ing. Viliam Novak 與本系土壤環
境領域相關同仁進行學術交流，互換心得。
(三) 人事異動消息：

本系李敏雄教授兼系主任暨所長任期屆滿，於 8 月起由李佳音教授擔任系主任
暨所長。

蘇南維助教於 8 月起聘為助理教授。

國科會以「博士後研究」資格，補助延攬大陸地區科技人士張學雷先生參與本
系研究。

劉王春花小姐升等為技工。
(四) 人力資源概況(九十學年度統計資料)

大學部：合計學生 384 人，大學部分土壤肥料組與農產製造組兩組。

研究所：合計學生 215 人，均各分五組，分別為土壤環境與植物營養組、生物
工業化學組、微生物學組、營養科學組和生物化學組，其中碩士班人數 134 人，
博士班人數 81 人。

榮譽教授：合計 5 人。

專任教師：合計 35 人。
教授

25 人
副教授
5人
助理教授
5人
兼任教授：合計 5 人。
— 2 —

技術人員及相關支援人員：合計 18 人。
技正、技士、技佐
技工、工友

12 人
6人
師生比：
年度 教師*
大學部
碩士班
博士班
學生總數
師生比
86
36
320
98
63
481
1：13
87
36
348
106
74
528
1：15
88
34
362
115
76
553
1：16
89
34
371
126
77
574
1：17
90
35
384
134
81
599
1：17
*註：不含合聘教師部分。
(五) 設備更新
1. 整修農化二館地下樓 B03 室為討論室可供討論、開會用途。
2. 於農化二館農化系辦公室 316 室增加闢建一間小型會議室。
3. 農化二館全棟門禁系統更新，變更刷卡方式使刷卡卡片與教職員證及學生證整合
成一張刷卡，並增加監測範圍。
4. 原系圖書館 416 室闢建為公用儀器室及貴重儀器室。
5. 本系各實驗室之環境保護及職業安全衛生工作相關資料，彙整製成電子檔以利查
詢更新。
6. 添購 LC-MS-MS (液相層析四極棒/飛行時間式串聯質譜儀)。
7. 添購全自動可程式蛋白質液相層析儀。
8. 添購 C.N.S.元素分析儀。
9. 添購氣相層析儀。
10. 添購光二極體矩陣檢測器。
11. 重新製作各教師名牌及各研究室標示牌。
— 3 —
第二項
統計表
(一) 教師人數統計表：
50
專任教師人數
(不含合聘教師 )
40
36
36
1997
1998
34
34
35
1999
year
2000
2001
30
20
10
0
(二) 學生人數統計表：
NUMBER OF STUDENTS
500
400
348
362
371
384
Undergraduate
students
320
300
200
161
180
191
203
215
100
0
1997
1998
1999
year
— 4 —
2000
2001
Graduate
students
(三) 研究成果統計表：
95
100
NUMBER OF PAPERS
87 87
80
91
84
Journal
Papers
70
58
60
54
Conference
Papers
40
19
20
0
1997
1998
1999
year
2000
2001
(四) 研究計畫統計表：
year
Number of Projects
Total Research of Grants
(million NTS)
1997
75
59,397
1998
79
58,193
1999
85
81,984
2000
49
39,641
2001
76
66,054
NUMBER OF PROJECT
TOTAL RESEARCH GRANTS (MILLION
NTS)
100
80
75
59
60
79
8582
76
66
Number of
Projects
58
49
40
40
Total Research
of Grants
(million NTS)
20
0
1997
1998
1999
year
2000
— 5 —
2001
— 6 —
界
業
學
小
企
及
商
工
門
部
中
及
科
專
位
單
政
行
府
政
位
所
單
究
研
府
政
究
研
人
究
百
分
私
研
及
學
大
(五) 畢業生就業狀況統計表：
40
30
20
比
10
0
第三項
研究計畫及經費來源
本系之研究計畫經費主要來自中央政府部門，其中以國科會、農委會及衛生署
為主要之資助機構。於 2001 年其他資助單位則有國立中興大學、財團法人南投縣建
設發展基金會及味全食品工業股份有限公司，共有 78 項研究計畫，總計畫經費為
68,424,300 元。

2001 年研究計畫一覽表
計畫
主持人
計畫名稱
補助單位
李佳音 PRTV 膠原蛋白於細菌中分泌能力之探討 3/3
國科會
李佳音 L-aspartate B -decarboxy-lase 重組酵素特性分析及其基因資訊研究
農委會
李佳音 腸炎弧菌磷脂絲胺酸合成脢基應用於 QPCR 檢測方法之研究
衛生署
林鴻淇 土壤生態.生物多樣性及地力增進之研究-土壤生態系中氮收支之研究
國科會
林鴻淇 堆肥的綜合肥效與土壤中養分的生物有效性之影響
農委會
王一雄 去除環境中殘留農用化學品之氯化物分解菌之培育與篩選之研究 1/3
國科會
王一雄 環境保護-苯胺及醯胺類型除草劑在土壤中之吸附及去除技術之研究 3/3
國科會
王一維 農藥環境毒理研究
農委會
李敏雄 豆腐製造程序中廢棄物減量之研究 2/2
國科會
李敏雄 以微生物生產黃色色素之研究(1/3)
國科會
李敏雄 洛神花花青素之分離及其抗氧化活性與血脂功能之研究
農委會
李敏雄 葡萄及楊桃釀造酒之研發
農委會
陳尊賢 陽明山國家公園之地形土序及其化育作用 1/2
國科會
陳尊賢
全球變遷:南仁山森林生態系研究-全球變遷:南仁山森林土壤水份含量及
土壤溶液組成之變
國科會
陳尊賢 九十年度農作物汙染監測及協助損害調處計畫補助
農委會
陳尊賢 台灣地區耕地之永續管理系統之建立(1/3)
農委會
陳尊賢 畜牧業處理過之廢水以土壤處理之技術及評估
農委會
王明光 塔塔加地區森林土壤生態養分動態及土壤調查(四)3/3
國科會
王明光 污染農地根圈土壤之重金屬生物有效性與復育管理 3/3
國科會
王明光 氣候變遷對林業影響適應技術之研究--對森林土壤古氣候的研究之二
農委會
王明光 林業溫室氣體排放資料庫之建立及更新(IV)
農委會
王明光 NMYU 伐對枯枝落葉分解及土壤理化性質的效應
農委會
鍾仁賜
經十一年不同輪作系統與施肥管理之土壤之磷素化學特性及對加入不同
肥料後之磷素轉變
國科會
鍾仁賜
土壤生態,生物多樣性及地力增進之研究-土壤生態系中植物之氮素營養特
性 1/3
國科會
鍾仁賜 加強肥料管理計畫-總胺基酸定量分析方法之探討
— 7 —
農委會
賴朝明
農業土壤生態,生物多樣性及地力增進之研究-土壤生態系中根圈酵素活性
之研究 1/3
國科會
賴朝明 台灣地區耕地之永續管理系統之建立(1/3)
農委會
李達源 應用階段調整式採樣結合地理統計推估污染場址中污染物之空間分佈
國科會
李達源 應用地理統計解析土壤中溶質傳輸之空間變異 2/2
國科會
李達源 台灣地區耕地之永續管理系統之建立(1/3)
農委會
顏瑞泓 有機磷殺蟲劑對映異構物之分離純化與生物毒性關係之研究
國科會
何聖賓 台灣地區耕地之永續管理系統之建立(1/3)
農委會
何聖賓 台灣酸性土壤石灰需要量的評估與模式之建立
農委會
財團法人南投縣
吳三和 福龜村有機農業推廣計畫
建設發展基金會
賴喜美 水分子移動性質對米糰與麵糰於糊化與凍藏儲藏期間質地變化之影響
國科會
賴喜美 油麵與涼麵衛生安全儲存品質改善之研究
衛生署
吳蕙芬 食物病源 Salmonella typhomusium clpy 基因表現與致毒調控蛋白之關係
衛生署
蘇仲卿 水稻澱粉磷解脢之分子生物學研究(第二年)
國科會
林良平 海藻天然保健食品之開發研究-微細藻株凝集素之篩選與生產程序之開發
國科會
林良平 生物性肥料開發與利用研究-生物肥料豆科根瘤菌增殖用培養資材之開發
農委會
林良平 利用黴藻去除養豬廢水中氮、磷之研究
農委會
宋賢一 植物蔗糖轉化之表現與調控研究 2/3
國科會
宋賢一 幾丁聚醣之純化與特性研究 2/3
國科會
劉文雄
利用植物固醇為原料以兩段式微生物轉換法生產 17-酮基類固醇化合物之
研究
國科會
楊盛行
溫室氣體之量測與減量對策-總計畫暨子計畫一:北部水稻田.旱田.魚類養
殖和濕地甲烷
國科會
楊盛行
塔塔加高山研究生態系-塔塔加高山研究生態系-塔塔加高山土壤微生物生
態分解 1.雲杉林土
國科會
楊盛行 提昇飼料及牧草品質之研究--高營養價值生物飼料之製備
農委會
楊盛行 生物性肥料開發與利用研究-高溫溶磷菌生物肥料之開發利用研究
農委會
楊盛行 加強畜牧污染防治及廢棄物處理
農委會
楊盛行 開發複合功能生物肥料之研究(1/3)
中興大學
李平篤 水稻蔗糖磷酯合成之生化學研究與生理學功能探討 2/3
國科會
黃青真 營養學門規劃研究推動計畫
國科會
黃青真 餵食炸鼠藉活化 PPARALPHA 調控其下游基因的表現 2/3
國科會
黃青真
PPARalpha 活化微粒體與過氧化體脂肪酸氧化系統於維生素 E 代謝之角色
1/3
莊榮輝 紅二次代謝物之免疫學測驗工具
國科會
國科會
重組類胰島素成長因子之量產.純化及對笛雕魚類成長之研究-重組類胰島
素成長因子之生產
國科會
黃健雄 利用酵素法生產特用 D 型胺基酸-Dht 及 DCase 之醱酵生產與純化及其在
國科會
黃健雄
— 8 —
特用 D 型胺基酸製程
蕭寧馨 過氧化體生成過氧化氫對細胞鐵調節蛋白質活性與鐵恆定狀況之影響
國科會
蕭寧馨 我國老人膳食鐵營養需要量研究
衛生署
味全食品工業
蕭寧馨 味全螺旋藻之護肝功能評估
股份有限公司
陳建源 低極化電位多項目電化學感測系統之開發研究
國科會
林璧鳳 維生素 E 與炸油對免疫細胞凋亡的影響 2/2
國科會
林璧鳳 微生物與植物食材各區分物對免疫功能的影響(3/3)
國科會
林璧鳳 菇菌類對過敏免疫功能調節的研究與開發
農委會
林璧鳳 我國老人膳食葉酸營養需要量研究
衛生署
潘子明 大腸桿菌 0157:H7 快速檢測與分子分形研究(2/3)
國科會
潘子明 基因改造大豆發酵食品-豆腐乳.味增及臭豆腐之分析研究
國科會
潘子明 豆腐乳、味增及臭豆腐等基因改良食品檢驗方法之研究
衛生署
許瑞祥 厭氣性真菌多樣性菌種資料庫建立及應用 1/3
國科會
王愛玉 糖與蛋白質磷酸化對水稻蔗糖合成基因及酵素功能之影響 2/3
國科會
劉俊民 本土性大腸桿菌 Escherichia coli 0157:h7 特性之研究
衛生署
黃慶璨
細胞與細胞之聯繫對 Pseudomonas aeruginosa 生物膜藻酸鹽生合成基因表
現之影響
國科會
楊健志
植物保護生技產業建立有關關鍵生物技術與資源之發展應用-以功能性基
因體分析選殖植物
國科會
楊健志 結構蛋白質體學研究計畫-植物膜蛋白乙烯受體之結構學研究
國科會
李昆達 藥用植物 ATROPA BELLADONNA 形質轉換根對水楊酸刺激之反應
國科會
李昆達 藥用植物八角蓮的細胞及組織培養
農委會
— 9 —
第四項
學術活動
(一) 2001 年參加國際學術會議情形
日
期
1 月 11-14 日
2 月 14-16 日
4 月 18-22 日
5 月 20-23 日
5 月 28 日~
6月
2日
7 月 1- 6 日
7 月 29 日~
8 月
2 日
8 月 5-11 日
8 月 26-31 日
8 月 27-31 日
10 月 8-12 日
10 月 12-16 日
10 月 15-16 日
10 月 23-24 日
10 月 28-30 日
11 月 26 日~
12 月
1 日
參
加
會
議
名
稱
The 3rd World Congress of
International Society for
Apheresis
26th International Stroke
Conference. AHA
第四次歐洲蛋白質學會研討
會
美國微生物學會 101 屆年會
第 18 屆亞太雜草學會會議
IUPAC International Congress
6th International Conference
on the Biogeochemistry of
Trace Elements
第三屆海峽兩岸植物分子生
物學及生物技術學術研討會
International Congress of
Nutrition
第 17 屆世界營養學大會
Soil Classification 2001:
Developing the basis for the
systems of the future
The 51th Annual Meeting of
the American Society of
Human Genetics
海峽兩岸首屆毒理學研討會
International Conference on
Biomedical Science & Food
Nutrition-Nobel Prize Forum
亞洲第一屆乳酸菌研討會
International Workshop on
Geographic Information
System in Soil Resources
Management
地
點
參加教師
補助單位
Taipei,
Taiwan
潘
文
涵
Fort
Lauderdale,
Florida,
U.S.A
法國巴黎巴
斯德研究所
美國奧倫多
北京
潘
文
涵
中 研 院
楊
健
志
自
潘
王
子
一
明
雄
國 科 會
教 育 部
澳洲
布里斯班
加拿大安大
略省 Guelph
市
香港中文大
學
維也納
楊
盛
行
國 科 會
李
王
達
一
源
雄
教 育 部
國 科 會
陳
慶
三
國 科 會
林
璧
鳳
國 科 會
維也納
Velence,
Hungary
黃
陳
青
尊
真
賢
中 研 院
國 科 會
California,
U.S.A.
潘
文
涵
中 研 院
南京
輔仁大學
王
黃
一
青
雄
真
國 科 會
韓國漢城
潘
子
明
Malila,
Philippines
陳
尊
賢
養樂多公
司
亞太糧肥
中心
— 10 —
費
(二) 2001 年舉辦國內外研討會及活動
日
期
5 月 3 日
5 月 12 日
研
討
會
(
或
活
動
名
)
稱
5 月 17 日
6 月 21 日
地理統計在農業和環境科學之應用研討會
「溫室氣體通量測定及減量對策(Ⅲ)」成果發
表研討會
生物科技在農業上之應用
第七屆土壤污染整治研討會
6 月 21 日
基因改造食品之檢驗與管理研討會
7 月 18 日
9 月 5 日
2001 年環境保護論壇
機能性醱酵製品研討會
9 月 13 日
11 月 2 日
11 月 13 日
12 月 14 日
12 月 27 日
新生入學指導
前進基因大世紀，生物產業教戰守則研討會
學術演講
土壤及地下水污染檢測講習會
品牌水果—楊桃研討會
斯洛伐克國家科學委員會院長 Drsc. Ing.
Viliam Novak 與土壤環境領域相關同仁進行
學術交流。
地
點
台大思亮館國際會議廳
台大農業陳列館
台大農業陳列館
台大第二學生活動中心
國際會議廳
台大第二學生活動中心
音樂廳
台大農業陳列館
台大第二學生活動中心
國際會議廳
台大圖書館國際會議廳
台北生物技術開發中心
台大思亮館國際會議廳
台大農業陳列館
本系
(三) 2001 年舉辦之學術專題演講
日
期
2 月 16 日
講
題
Moderm Approaches for the
Antibiotic Discovery Research
5 月 16 日
演
講
人 服
吳群也
先生
Regulation of Fat Intake by the
Peptide Enterostatin
5 月 30 日 生物晶片與農業科技
6 月 6 日 Structure and Expression of an
Amlopullulanase Gene From
Bacillus Stearothermophilus TS-23
10 月 22 日 Antioxidants, NFkB and
Diabetogenesis
Dr. David
York
籐涵菁博士
朱文深
博士
10 月 22 日 Chemical and Bionutritional
Aspects of Carotenoids
Dr. Steven
Schwartz
12 月 3 日 Recent Progress in Recombinant
Protein Production
王淑媛博士
Dr. Tammy
Bray
— 11 —
務
單
位
Associate Senior Microbiogist
Infections Diseases Division Eli
Lilly Research Laboratories
美國路易西安那州立大學
Pennington 生醫中心主任
晶宇生物科技實業股份有限公司
食品工業發展研究所菌種保存
及研究中心副主任
Associate Dean and Professor,
Department of Human Nutrition,
College of Human Ecology, The
Ohio State University
Professor of Food Science and
Technology, The Ohio State
University
美國 Amgen 生技公司研究員
第五項
期刊論文、研討會論文等研究成果
李佳音
◎期刊論文
1. Su, S.C. and C.Y. Lee. 2001. Cloning of the N-acylamino acid racemase gene from
Amycolatopsis azurea and biochemical characterization of the gene product. Enzyme
Microb. Technol. 30:647-655.(SCI)
2. Lee, C.Y., M.F. Cheng, M.S. Yu and M.J. Pan. 2001. Purification and characterization
of a putative virulence factor, serine protease, from Vibrio parahaemolyticus. FEMS
Microbiol. Lett. 209:30-36.(SCI)
◎研討會論文
1. Lin, P. and C.Y. Lee. 2001. Gene cloning, protein expression, purification and
characterization of D-aminoacylase from Variovorax paradoxus ISO1. Abstr. P.46.
ASM Conference on Biodegradation, Biotransformation, and Biocatalysis (B3), San
Juan, Puerto Rico.
2. 李佳音。2001。高分子生物可分解塑膠生產菌之開發。生物科技在農業上之應用
85-94。
3. 林奐妤、李佳音。2001。腸炎弧菌膠原蛋白酵素基因調控與蛋白質表現。第三十
五次中華民國微生物學會會員大會 75。國立陽明大學。
◎ 專利
1. 中華民國專利「鑑定腸炎弧菌之引子及探針」，專利號碼 088863 號。
洪崑煌
◎期刊論文
1.
Lin, T.H., C.L. Chen and K.H. Houng. 2001. Interpretation of the Charge
Characteristic in terms of constant capacitance and diffuse double layer models.
Food Sci. Agr. Chem. 3: 121-126.
王一雄
◎期刊論文
1. Chiang, H.C., J.R. Duh and Y.S. Wang. 2001. Butachlor, thiobencarb and
chlomethoxyfen movement in subtropical soils. Bull. Environ. Contam. Toxicol. 66(1):
— 12 —
1-8. (SCI)
2. Chen, I.M., F.C. Chang, M.F. Hsu and Y.S. Wang. 2001. Comparisons of PCBs
dechlorination occurences in various contaminated sediments. Chemosphere 43(7):
649-654. (SCI)
3. Chen, I.M., F.C. Chang and Y.S. Wang. 2001. Correlation of gas chromatographic
properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of
reductive dechlorination by untamed microorganisms. Chemosphere 45(2): 223-229.
(SCI)
4. Yen, J.H., C.C. Tsai, C.C. Su and Y.S. Wang. 2001. Environmental dissipation of
fungicide triphenyltin acetate and its potential as a groundwater contaminatant.
Ecotoxicol. Environ. Safe. 49: 164-170. (SCI)
5. Chen, H.W., J.H. Yen, R.S. Chung, C.M. Lai, S.S. Yang and Y.S. Wang. 2001. Carbon
dioxide flux density ini cultivated rice paddy field. Proc. Natl. Sci. Counc. ROC (B)
25(4): 239-247.
6. Chen, I.M., B.V. Chang, S.Y. Yuan and Y.S. Wang. 2001. Reductive dechlorinatioon of
hexachlorobenzene under various additions. Water, Air, and Soil Pollution 139 (1-4):
61-74(SCI)
7. Lin, K.Y., J.H. Yen and Y.S. Wang. 2001. Characterization of fungicides nabam and
zineb in alkaline solution and a simple determination method for them. Food Sci. Agric.
Chem. 3(4):127-130.
◎研討會論文
1. Sheu, W.S., J.H. Yen and Y.S. Wang. 2001. Dissipation of herbicide oxyfluorfen in
subtropical soils and its potential to contaminate groundwater. Paroceedings II, The
18th Asian-Pacific Weed Science Socitty Conference 888. Beijing, P. R. China.
2. Yen, J.H., H.C. Chen and Y.S. Wang. 2001. Dissipation of aniline herbicide trifluralin
and benfluralin in soils. Paroceedings II, The 18th Asian-Pacific Weed Science Socitty
Conference 892. Beijing, P. R. China.
3. 張秀齡、顏瑞泓、王一雄。2001。醯銨類除草劑在不同土壤中的吸附、降解及移
動之研究。中國農業化學會第三十九次會員大會論文摘要102，E-12。台北。
4. 張芳誠、顏瑞泓、王一雄。2001。多氯聯苯曝露於紫外光下之降解情況與途徑。
中國農業化學會第三十九次會員大會論文摘要 103，E-13。台北。
5. 方鶴岩、顏瑞泓、王一雄。2001。除草劑達有龍、理有龍、愛速隆及甲基合氯氟
在土壤中之消散。中國農業化學會第三十九次會員大會論文摘要 112，E-31。台
— 13 —
北。
6. Wang, Y.S., K.Y. Lin and J.H. Yen. 2001. Propeteies of metal-containing ethylene
bisdithiocarbamate fungicides nabam and zineb in alkaline solutiono. Proceedings of
ICOBTE 2001 conference (GP 128) 409. 6th International coonference on the
Biogeochemistry of Trace Elements, University of Guelph, Guelph, Ontario, Canada.
7. 王一雄。2001。台灣地區農業環境毒理研究概況。海峽兩岸首屆毒理學研討會論
文摘要集8。南京。
◎專書論文
王一雄、陳尊賢、李達源。2001。土壤資源利用與保育。國立空中大學用書，國立
空中大學印行。台北。
李敏雄
◎期刊論文
1.
Su, N.W. and M.H. Lee. 2001. Purification and characterization of a Novel
Salt-tolerant Protease from Aspergillus sp. FC-10, a soy sauce koji mold. Journal of
Industrial Microbiology and Biotechnology 26: 253-258. (SCI)
2.
Su, N.W. and M.H. Lee. 2001. Screening and characterization of koji molds
producing saline-tolerant protease. Journal of Industrial Microbiology and
Biotechnology 26: 230-234. (SCI)
3.
鍾玉明、黃寶雄、蘇南維、李敏雄。2001。酒中醋酸之快速氣相層析定量法。
台灣農業化學與食品科學，39:231-236。
◎研討會論文
1. 蔡珮新、李敏雄。2001。黑后葡萄酒製備之研究。中國農業化學會第三十九次會
員大會手冊 39。
2. 高馥君、李敏雄。2001。豆水回添豆腐製備之研究。中國農業化學會第三十九次
會員大會手冊 40。
3. 林姿伶、李敏雄。2001。由 Gordonia terrae FC-OR1 生產類胡蘿蔔色素之研究。
中國農業化學會第三十九次會員大會手冊 40。
4. 鍾玉明、翁順祥、蘇南維、李敏雄。2001。云香科水果果皮自體酵素作用精油成
分之研究。中國農業化學會第三十九次會員大會手冊 49。
5. 鍾玉明、李敏雄。2001。脂肪醇與酒石酸之反應產物組成及其基本特性之探討。
中華民國食品科學技術學會第三十一次會員大會手冊 208。
6. Choong, Y.M., M.C. Lin and M.H. Lee. 2001. Immobilized lipase-catalyzed synthesis
— 14 —
of hexyl acetate by direct esterification. Handbook of Annual meeting of the Chinese
Institute of Food Science and Technology 208.
7. 吳貞瑩、李敏雄。2001。紅龍果紅色色素之研究。中華民國食品科學技術學會第
三十一次會員大會手冊 227。
陳尊賢
◎期刊論文
1. Hseu, Z.Y. and Z.S. Chen. 2001. Quantifying soil hydromorphology of a rice-growing
Ultisol toposequence in Taiwan. Soil Sci. Soc. Am. J. 65 (1): 270-278 (SCI)
2. Hseu, Z.Y. and Z.S. Chen. 2001. Quantifying soil hydromorphology of a rice-growing
Ultisol toposequence in Taiwan. Soil Sci. Soc. Am. J. 65(1): 270-278 (SCI)
3. Chen, Z.S., T.C. Tsou, V.B. Asio and C.C. Tsai. 2001. Genesis of Inceptisols on a
volcanic landscape in Taiwan. Soil Sci. 166(4): 255-266. (SCI)
4. Tsai, C.C., Z.S. Chen, C.T. Duh and F.W. Horng. 2001. Prediction of soil depth using a
soil-landscape regression model: Case study of the forest soils of southern Taiwan.
Proc. National Sci. Council, ROC. Part B: Life Sci. 25(1): 34-39.
5. 許正一、陳尊賢、蔡呈奇、崔君至。2001。以微形態學探討南仁山區熱帶森林土
壤之化育作用。台灣林業科學，16(1): 25-38.
6. Tsai, C.C. and Z.S. Chen. 2001. Soil characteristics and variability of an Ultisol
toposequence in Nanjenshan subtropical forest ecosystem of Taiwan. Food Sci. &
Agric. Chem. 3(1): 13-22.
◎研討會論文
1. Chen, Z.S., Z.Y. Hseu, S.P. Wu and C.C. Tsai. 2001. Transition soils between
Spodosols and Ultisols in subalpine forest of Taiwan. Soil Classification 2001:
Developing the basis for the systems of the future. Velence, Hungary.
2. 賴鴻裕、陳尊賢。2001。污染土壤之植物復育技術。第七屆土壤污染整治研討會
論文集 145-170。陳尊賢(主編)。國立臺灣大學第二活動中心國際會議廳。台北市。
◎專書論文
1. Chen, Z.S., J. Bay-Petersen, and W.T.F. Chiu (editors). 2001. Micronutrient deficiency
of crop in Asia. Published by Food and Fertilizer Technology Center, Taipei, Taiwan
ROC. p.111.
— 15 —
王明光
◎期刊論文
1. Chen, M.C., M.K. Wang, C.Y. Chiu, P.M. Huang and H.B. King. 2001. Determination
of low molecular weight dicarboxylic acids and organic functional groups in
rhizosphere and bulk soils in a temperate forest soils. Plant and Soil. 31: 37- 44. (SCI)
2. Juang, T.C., M.K. Wang, H.C. Chen and C.C. Tan. 2001. Ammonium fixation by
surface soils and clays. Soil Sci. 166: 345-356. (SCI)
3. Chang, C.M., M.K. Wang, T.W. Chang and C. Lin. 2001. Transport modeling of copper
and cadmium with linear and nonlinear retardation factors. Chemosphere 43:
1133-1139. (SCI)
4. Yang, D.S. and M.K. Wang. 2001. Characterization and a fast method to synthesis of
sub-micron lithiophorite. Chemical of Materials 13: 2589-2594. (SCI)
5. Chen, P.Y., M.K. Wang and D.S. Yang. 2001. Mineralogy of dickite and nacrite from
northern Taiwan. Clays and Clay Minerals 49,(6):586-595. (SCI)
6. Liu, C.L., M.K. Wang and C.C. Yang. 2001. Determination of cation-exchange
capacity by one-step soil leaching column method. Comm. Soil Sci. and Plant Analy.
32(15&16): 2359-2372. (SCI)
7. Chiu, C.Y., M.K. Wang, H.B. King and J.L. Hwong. 2001. Physical and chemical
properties of rhizosphere and bulk soils of Tsuga and Yushania in a temperate forest
soils. Commun. Soil Sci. Plant Anal. 33 (11-12): 1723-1735. (SCI)
8. Chang, T.W., M.K. Wang and C. Lin. 2001. Adsorption in the different sorbent/water
ratios of soil systems. Water, Air, and Soil Pollution. 138 (1-4): 199-209 ( SCI)
9. Chen, H.J., Y.M. Tzou, T.C. Juang and M.K. Wang. 2001. Effect of net surface charge
and NH4+ exchange selectivity on ammonium fixation of soil clays. Soil and
Environment 4(2) 93-104.
鍾仁賜
◎期刊論文
1. Chen, H.W., J.H. Yen, R.S. Chung, C.M. Lai, S.S. Yang and Y.S. Wang. 2001. Carbon
dioxide flux density in cultivated paddy rice field. Proc. Natl. Sci. Counc ROC (B), 25:
239-247.
2. 王斐能、蘇育萩、鍾仁賜。2001。不同的堆肥對生長在紅壤之青梗白菜養分吸收
— 16 —
的影響。土壤與環境，3: 183-192。
◎ 研討會論文
1. Chung, R.S., R.C. Shieh, H.S. Hsu and Y.H. Tsai. 2001. Nutrient released from soil
after application of different fertilizers for 11 years in two rotation systems. Proceeding
of 12th World Fertilizer Congress, 12th World Fertilizer Congress 12th World Fertilizer
Congress 6, Beijing. (in press)
2. 鍾仁賜、曾日昌、徐華盛、蔡永皞。2001。經長期(十一年)不同施肥管理後之土
壤對作物生長與養分吸收的影響。有機肥料與合理化施肥研討會 37-40，中華土
壤肥料學會，台中。
李達源
◎期刊論文
1. Juang, K.W., D.Y. Lee and T.R. Ellsworth. 2001. Using rank order geostatistics for
spatial interpolation of highly skewed data in a heavy metal contaminated site. J.
Environ. Qual. 30: 894-903. (SCI)
◎研討會論文
1.Juang, K.W., Y.S. Chen and D.Y. Lee. 2001. Use of geostatistical simulation for
assessing the spatial uncertainty of mapping heavy-metal contents in soils. Proceedings
of Extended Abstracts, 6th International Conference on the Biogeochemistry of Trace
Elements. 521. University of Guelph, Guelph, Ontario, Canada.
2.Lee, D.Y., P.N. Liao and K.W. Juang. 2001. Fractionation and bioavailability of copper
and zinc in four Taiwan soils amended with sewage sludges. Proceedings of Extended
Abstracts, 6th International Conference on the Biogeochemistry of Trace Elements.
421. University of Guelph, Guelph, Ontario, Canada.
3.吳榮富、莊愷瑋、李達源。2001。以排序性階段調整叢集採樣結合克力金法推估
土壤中之重金屬分佈。第七屆土壤污染防治研討會論文集 181。
4.李達源、莊愷瑋。2001。地理統計於重金屬污染場址危害範圍界定之應用。地理
統計在農業和環境科學之應用研討會論文集 57-78。
◎專書
1. 王一雄、陳尊賢、李達源。2001。土壤資源利用與保育。國立空中大學印行。
1. 李達源(編)。2001。地理統計在農業和環境科學之應用研討會論文集。中國農業
化學會印行。
— 17 —
顏瑞泓
◎期刊論文
1. Yen, J.H., C.C. Tsai, C.C. Su and Y.S. Wang. 2001. Environmental dissipation of
fungicide triphenyltin acetate and its potential as a groundwater contaminatant.
Ecotoxicology and Environmental Safety 49: 164-170. (SCI)
2. Chen, H.W., J.H. Yen, R.S. Chung, C.M. Lai, S.S. Yang and Y.S. Wang. 2001.
Carbon dioxide flux density in cultivated rice paddy field. Proceedings of the National
Science Council, ROC, Part B: Life Sciences 25(4): 239-247.
3. 許渭鑫、王一雄、顏瑞泓。2001。除草劑伏寄普在茶園土壤中的消散。土壤與環
境。4(4):279-290。
4. 顏瑞泓、謝國慶、王一雄。2001。有機磷殺蟲劑賽達松鏡像異構物之分離與毒性。
台灣農業化學與食品化學。4(40)。
賴喜美
◎期刊論文
1. Lai, H.M. 2001. Effects of hydrothermal treatment on the physicochemical properties
of pregelatinized rice flour. Food Chemistry 72: 455-463. (SCI)
◎研討會論文
1.鍾依林、賴喜美。2001。雙醣分子對澱粉凝膠之熱性質與分子移動性質之影響。
第 39 屆中國農業化學會年會，台北，台灣。
2.黃傳雄、賴喜美。2001。糊化條件對高直鏈澱粉含量米穀粉之凝膠性質與質地之
影響。第 39 屆中國農業化學會年會，台北，台灣。
3.Lai, H.M. and Y.L. Chung. 2001. Molecular mobility of waxy rice starch paste at low
temperature studied by using MDSC and NMR. 83th Annual Meeting of The American
Association of Cereal Chemists, Charlotte, North Carolina, U.S.A.
4.Tseng, C.S. and H.M. Lai. 2001. Effects of MTGase on the modification of flour
dough properties studied by response surface method. 83th Annual Meeting of The
American Association of Cereal Chemists, Charlotte, North Carolina, U.S.A.
5.鍾依林、賴喜美。2001。雙醣對冷凍熟麵品質之影響。第三十一屆食品科學年會，
嘉義，台灣。
— 18 —
吳蕙芬
◎研討會論文
1. 李宜穎、張瓊方、吳蕙芬。2001。In vivo protein-protein interactions involved in
subunits oligomerization and substrates recognition of the Escherichia coli ClpYQ
(HslUV) protease by the yeast two-hybrid.中國農業化學第 39 次會員大會壁報論文。
蘇南維
◎期刊論文
1. Su, N.W. and M.H. Lee. 2001. Purification and characterization of a novel salt-tolerant
protease from Aspergillus sp. FC-10, a soy sauce koji mold. Journal of Industrial
Microbiology and Biotechnology 26: 253-258. (SCI)
2. Su, N.W. and M.H. Lee. 2001. Screening and characterization of koji molds producing
saline-tolerant protease. Journal of Industrial Microbiology and Biotechnology 26:
230-234. (SCI)
3. 鍾玉明、黃寶雄、蘇南維、李敏雄。2001。酒中醋酸之快速氣相層析定量法。台
灣農業化學與食品科學，39: 231-236。
◎研討會論文
1. 鍾玉明、翁順祥、蘇南維、李敏雄。2001。云香科水果果皮自體酵素作用精油成
分之研究。中國農業化學會第 39 次會員大會
林良平
◎ 期刊論文
1.
林良平。2001。光學顯微鏡之最新進展－無限遠補正光學系統。科儀新知，22(4):
6-11。
2.
Wu, H.L., R.S. Hseu and L.P. Lin. 2001. Utilization of ribosomal DNA sequences to
identify Chlorella spp. isolates. Bot. Bull. Acad. Sin. 42:115-121. (SCI)
3.
胡淳怡、林良平。2001。滲透壓保護物對根瘤菌之影響。科學農業，49: 49-55。
4.
Liu, C.P. and L.P. Lin. 2001. Ultrastructural study and DHA production of Isochrysis
sp. CCMP1324. Bot. Bull. Acad. Sin. 42: 207-214. (SCI)
◎研討會論文
1. Lin, L.P. 2001. Mixotrophic mass culturing of Chlorella cells for health foods. The
— 19 —
11th World Congress Food Sci. Tech., Seoul, Korea.
2. Lin, L.P. 2001. Mixotrophic growth of Chlorella spp. Asian- Pacific Meet. Phycol.
Yamagata, Japan.
3. Lin, L.P. 2001. Signification of microalgal mass cultivation in the subtropics of
Taiwan. 2nd Intrn’l Symp. Biotechnology of Tropical & Subtropical Species. Taipei,
Taiwan, R.O.C.
4. Chen, S.L., C.Y. Chu., W.R. Liao, R. Huang and L.P. Lin. 2001. Hemagglutination,
antibiotic activity and identification of Chlorella spp. isolates. T59, The 2001 Ann. Met.
Fish. Soc. Taiwan & Symp. New Fron. Marine Biotech. Kaohsiung.
5.
Chung, C.C., K.Y. Chen, W.R. Liao, J.Y. Lin, Y.M. Chiang, L.P. Lin and R. Huang.
2001. Agglutinating activity from marine algae of Taiwan. T60, The 2001 Ann. Met.
Fish. Soc. Taiwan & Symp. New Fron. Marine Biotech. Kaohsiung, Taiwan.
6. Hu, C.Y., C.Y. Chen, W.Y. Lee, S.M. Lin and L.P. Lin. 2001. The morphogensis of
Sinorhizobium fredii and confirmation of cellulytic and pectinolytic enzymes. 2001
International Workshop on Advances Microscopy. Hsinchu, Taiwan.
7.
Chu, C.Y., S.L. Chen, W.R. Liao, R. Huang and L.P. Lin. 2001. Screening
hemagglutinins of freshwater microalgae with animal and human erythrocytes. The
35th Ann. Met. Chin. Soc. Microbiol.
8. Chu, C.Y., S.L. Chen, Y.S. Lai, W.R. Liao, R. Huang and L.P. Lin. 2001.
Hemagglutination and antibacterial activity of Chlorella spp. isolates. The 35 th Ann.
Met. Chin. Soc. Microbiol. Taipei, Taiwan.
9. 劉清標、林良平。2001。利用小球藻去除養豬廢水中氮、磷及重金屬。第 26 屆
廢水處理技術研討會 A-40。高雄，台灣。
10. 劉清標、林良平。2001。微藻對養豬廢水氮、磷之去除效應。第四屆畜牧資源回
收再利用研討會。台中，台灣。
◎ 專利
1. 日本發明專利「過濾器、過濾方法及過濾裝置」，專利號碼 H10-152081 號。
2. 日本發明專利「小球藻大量培養之新方法及裝置」，專利號碼 H9-173262 號。
劉文雄
◎期刊論文
1.
Lo,
C.K.,
K.L.
Wu
and
W.H.
Liu.
2001.
Interconversion
of
androst-4-ene-3,17-dione and testosterone using enzyme or resting cells of
— 20 —
Mycobacterium sp. Food Sci. Agric. Chem. 3: 30-35.
2.
Tang, T.Y., J.G. Jong and W.H. Liu. 2001. Purification and some properties of
sarcosine dehydrogenase from Pseudomonas putida RS65. Food Sci. Agric. Chem.
3: 36-41.
◎討論會論文
1. 黃承烺、潘兆平、劉文雄。2001。利用分枝桿菌由植物固醇生產荷爾蒙中間體雄
烯二酮化合物之研究。第六屆生化工程研討會 35-38，中央大學，中壢，台灣。
◎技術報告
1. 劉文雄。2001。利用植物固醇為原料以醱酵法生產 17-酮基類固醇化合物之研究。
2. 劉文雄。2001。利用任意突變技術改良及解析假單胞桿菌 RS65 肌酸美酶基因在
大腸桿菌中之表現。
楊盛行
◎期刊論文
1.
Yang, S.S. and H.L. Chang. 2001. Effect of green manure amendment and flooding
on methane emission from paddy fields. Chemosphere: Global Change Science 3:
41-49. (SCI)
2.
Yang, S.S. and H.L. Chang. 2001. Methane emission from paddy fields in Taiwan.
Biology and Fertility of Soils 33: 157-165. (SCI)
3.
Yang, S.S. and C.M. Lee. 2001. Formation and regeneration of protoplasts for
protease production in Streptomyces rimosus. Journal of Microbiology, Immunology
and Infection 34: 8-16.
4.
Yang, S.S. and C.M. Lei. 2001. Effect of culture media on protease and
oxytetracycline production in Streptomyces rimosus. World Journal of Microbiology
& Biotechnology 17: 403-410. (SCI)
5.
Yang, C.K. and S.S. Yang. 2001. Microbial ecology of soils surrounding nuclear and
thermal power plants in Taiwan. Environment International 26: 315-322.(SCI)
6.
Yang, S.S., H.T. Yang, W.R. Chen and C.B. Wei. 2001. Properties of livestock and
poultry wates during composting in the farms of northern Taiwan. Journal of the
Chinese Environmental Society 24: 8-25.
7.
Yang, S.S., C.M. Liu and Y.L. Liu. 2001. Estimation of methane and nitrous oxide
emission from animal husbandry industry on Taiwan. Journal of the Agricultural
Association of China 2: 450-463.
— 21 —
8.
Chen, H.W., J.H. Yen, R.S. Chung, C.M. Lai, S.S. Yang and Y.S. Wang. 2001.
Carbon dioxide flux density in cultivated rice paddy field. Proceedings of National
Science Council, ROC, Part B. 25: 239-247.
9.
Yang, S.S. and C.Y. Yueh. 2001. Oxytetracycline production by immobilized
Streptomyces rimosus. Journal of Microbiology, Immunology and Infection
34:235-242
10. Chang, C.H. and S.S. Yang. 2001. Effect of cultural media on the
phosphate-solubilizing activity of thermo-tolerant microbes. Journal of Biomass
Energy Society of China 20:79-90.
11. Jang, H.D. and S.S. Yang. 2001. Greenhouse gases production of municipal solid
wastes in column bioreacters. Journal of Biomass Energy Society of China
20:101-112.
◎研討會論文
1. Yang, S.S., C.M. Liu and Y.L. Liu. 2001. Methane and nitrous oxide emissions from
agricultyre sector in Taiwan. In: Proceedings of the Seminar on Taiwan’s National
Greenhouse Gases Inventories. 5-1~5-19. Environmental Protection Administration,
Government of the Republic of China and Energy & Resources Laboratories of
Industrial Technology Research Institute, Taipei, Taiwan, ROC.
2. Yang, S.S. 2001. Application of solid state fermentation on the production of
agriculture and industry. In: Proceedings of the Seminar of Microbial Technology in
Taiwan. Department of Food Sciences and Technology,49-67. National Taiwan
Marine University, Keelung, Taiwan, ROC.
3. Yang, S.S. 2001. Flux and mitigation of greenhouse gases (III). In: Flux and
Mitigation of Greenhouse Gases (III). 1-39. Global Change Research Center,
Department of Agricultural Chemistry and Agricultural Exhibition Hall, National
Taiwan University, Taipei, Taiwan, ROC.
4. Yang, S.S. 2001. Methane fluxes and mitigations of landfills in northern Taiwan (iii):
flux and diurnal variation of greenhouse gas emission. In: Flux and Mitigation of
Greenhouse Gases (III).41-56. Global Change Research Center, Department of
Agricultural Chemistry and Agricultural Exhibition Hall, National Taiwan University,
Taipei, Taiwan, ROC.
5. Yang, S.S. and U. Hegde. 2001. Greenhouse gas production during anaerobic digestion
of municipal solid waste. In: Flux and Mitigation of Greenhouse Gases (III). 57-67.
— 22 —
Global Change Research Center, Department of Agricultural Chemistry and
Agricultural Exhibition Hall, National Taiwan University, Taipei, Taiwan, ROC.
6. Hegde, U., T.C. Chang and S.S. Yang. 2001. Greenhouse gas emissions from
Shan-Chu-Ku landfill site in northern Taiwan. In: Flux and Mitigation of Greenhouse
Gases (III). 69-83. Global Change Research Center, Department of Agricultural
Chemistry and Agricultural Exhibition Hall, National Taiwan University, Taipei,
Taiwan, ROC.
7. Chang, E.H. and S.S. Yang. 2001. Biofixation of carbon dioxide by microalgae
isolated in Taiwan. In: Flux and Mitigation of Greenhouse Gases (III). 219-236.
Global Change Research Center, Department of Agricultural Chemistry and
Agricultural Exhibition Hall, National Taiwan University, Taipei, Taiwan, ROC.
8. Yang, S.S. 2001. Application of microbial technology on waste treatment. In:
Proceedings of the Seminar of Biological Technology on Agriculture.15-16.
Agricultural Exhibition Hall and Department of Agricultural Chemistry, National
Taiwan University, Taipei, Taiwan, ROC.
9. Yang, S.S. 2001. Environmental protection and agricultural production. In:
Proceedings of the Seminal on Sustainable Agriculture Production. The Chinese
Sustainable Agriculture Society, Taichung, Taiwan, ROC.
10. Yang, S.S. 2001. Isolation and technology of multiple functional biofertilizer and
compost. In: Proceedings of the Forum of Environmental Protection. Agricultural
Exhibition Hall of National Taiwan University and National Program of Agricultural
Biotechnology, Taipei, Taiwan, ROC.
11. Yang, S.S. and M.Y. Chang. 2001. Introduction to the cultivation, physiology and
biochemical properties of star fruit. In: Proceedings of the Seminar on Well-Know
Fruit: Star Fruit. 1-7. Department of Agricultural Chemistry and Agricultural
Exhibition Hall, National Taiwan University, Taipei, Taiwan, ROC.
12. Yang, S.S. and C.H. Chang. 2001. Effect of culture media on thermophilic
phosphate- solubilizing activity. In: Proceedings of the 4th Symposium on Resources
Recovery and Re-utilization of Livestock. National Chung-Hsin University, Taichung,
Taiwan, ROC.
13. Yang, S.S. 2001. Application of solid state fermentation in waste treatment. In
Program and Abstracts of Annual Meeting of Environment Protection Society of ROC
in 2001.15-25. National Taiwan University, Taipei, Taiwan.
— 23 —
◎專書論文
1. Yang, S.S. 2001. Flux and Mitigation of Greenhouse Gases (III). Global Change
Research Center, Department of Agricultural Chemistry and Agricultural Exhibition
Hall, National Taiwan University, Taipei, Taiwan, ROC. p. 1-239.
2. Yang, S.S. 2001. Application of Biological Technology on Agriculture. Department of
Agricultural Chemistry and Agricultural Exhibition Hall, National Taiwan University,
Taipei, Taiwan, ROC. p. 1-423. ISBN-957-02-9599-6.
3. Yang, S.S. 2001. Application of Microbial Technology on Waste Treatment. In:
Application of Biological Technology on Agriculture. Agricultural Exhibition Hall and
Department of Agricultural Chemistry, National Taiwan University. 95-124. Taipei,
Taiwan, ROC. p. 1-423. ISBN-957-02-9599-6.
◎技術報告
1. Yang, S.S. and S.H. Tsai. 2001. Treatment and Re-utilization of Food Wastes.
Legislative Yuan Monthly. (in press).
李平篤
◎期刊論文
1. Chen, W.L., D.J. Hung, H.L. Wang, J.C. Su and P.D. Lee. 2001. Purification and
characterization of sucrose phosphase synthase from sweet potato tuber roots. Bot.
Bull. Acad. Sin. 42 (2): 123-129.(SCI)
◎研討會論文
1. Hung, D.J., J.C. Su and P.D. Lee. 2001. Purification and Characterization of Sucrose
Phosphate Synthase from Sweet Potato Tuberous Roots. The 39th Annual Meeting of
the Chinese Agri. Chem. Society.
黃青真
◎期刊論文
1. 趙蓓敏、黃青真。2001。15-deoxy-Δ12,14-PGJ2 對 3T3-L1 前脂肪細胞分化之影
響。中國醫藥科學雜誌，2: 117-124。
2. Wu, M.C. and C.J. Huang. 2001. Inhibition of prostaglandin e2 production of a
macrophage cell line by some phytochemicals. Fd. Sci. Agric. Chem. 3: 59-71.
3. Chao, P.M., C.Y. Chao, F.J. Lin and C.J. Huang. 2001. Oxidized frying oil up-regulates
— 24 —
hepatic acyl coa oxidase and cytochrome P-450 4 A1 genes in rats and activate
PPAR-. J. Nutr. 131: 3166-3174. (SCI)
◎研討會論文
1. 李亦臻、黃青真。2001。比較黃豆油、芥花油、及牛脂對大鼠肝臟中
游基因及 cytochrome P-450
下
27 屆
年會暨學術研討會手冊 104。國防醫學中心。
2. 鄭瑋宜、黃青真。2001。飲食炸油對大鼠體內水分及鈉鉀平衡之影響。中華民
國營養學會第 27 屆年會暨學術研討會 104。國防醫學中心。
3. Huang, C.J. 2001. PPAR and Atherosclerosis.中華民國血脂及動脈硬化學會九十年
度學術研討會會刊 33。台北榮總致德樓第二會議室。
4. Chao, P.M., C.Y. Chao and C.J. Huang. 2001. Upregulation of hepatic acyl CoA
oxidase and cytochrome P-450 4A Genes through PPAR-α activation in rats fed
oxidized frying oil. International Conference on Biomedical Science & Food
Nutrition—Nobel Prize Forum. Handbook. 34. Fu-Jen Catholic University.
5. 黃青真。2001。影響前列腺素 E2 生合成之食物或實務成分。機能性食品研討會，
國立嘉義大學國際會議廳。
莊榮輝
◎期刊論文
1. Chen, H.M., S.C. Chang, C.C. Wu, T.S. Cuo, J.S. Wu and R.H. Juang. 2001. The
catalytic behavior of L-form starch phosphorylase from sweet potato roots is regulated
by proteolysis. Physiol. Plantarum. 114(4): 506-515. (SCI)
黃健雄
◎期刊論文
1.
黃健雄、胡紹揚。2001。利用重組大腸菌以全合成饋料液饋料批式培養生產
N-Carbamoyl-D-Amino Acid Amidohydrolase。中華生質能源學會會誌，20: 33-45。
◎研討會論文
1.
胡紹揚、黃健雄、吳金洌。2001。吳郭魚第二型類胰島素成長因子之生產。中
國農業化學會年會演講摘要 38。
2.
洪嘉佑、胡紹揚、黃健雄。2001。重組類胰島素成長因子第一型之生產與純化。
中國農業化學會年會演講摘要 38。
— 25 —
3.
Huang, J.H., W.H. Hsu and C.H. Hsu. 2001. Production of thermostable
D-hydantoinase from recombinant Escherichia coli. Abstracts of the ASM
Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan,
Puerto Rico.
蕭寧馨
◎研討會論文
1. 王瑞蓮、高美丁、潘文涵、蕭寧馨。2001。台灣地區成人膳食營養品質評估：飲
食健康指數(HEI; Health Eating Index). 中華民國營養學會第廿七屆年會，台北市。
2. 黃惠玲、蕭寧馨。2001。膳食缺鐵透過 IRPs 影響 LEC 大鼠肝臟體蛋白與粒線體
aconitase 表現。中華民國營養學會第廿七屆年會，台北市。
3. Huang, H.L. and N. Shaw. 2001. Differential modulation of hepatic IRP1 and IRP2
activities in rats fed oxidized oil and fish oil. 17th International Congress of Nutrition,
Vienna, Austria.
4. Huang, H.L. and N. Shaw. 2001. Hypolipidemic drug clofibrate alters serum iron
transport and hepatic iron and copper metabolism in rats fed a high fat diet. 17th
International Congress of Nutrition, Vienna, Austria.
陳建源
◎期刊論文
1. Chen, C.Y., W.T. Wu, M.H. Lin, C.K. Ch’ang, H.J. Huang, J.M. Liao, L.Y. Chen and
Y.T. Liu. 2001. A common precusor for the three subunits of L-glutamate oxidase from
Streptomycess platensis NTU3304. Canadian Journal of Microbiology. 47:269-275.
(SCI)
2. Chou, S.F. and C.Y. Chen. 2001. Production and purification of monoclonal and
polyclonal antibodies against human ferritin, a nonspecific tumor marker. Hybridoma
20(1):59-62. (SCI)
3. Chou, S.F., W.L. Hsu, C.M. Hwang and C.Y. Chen. 2001. Development of an
immunosensor of human ferritin, a nonspecific tumor marker. Analytica Chimica Acta,
453 (2): 181-189. (SCI)
4. 周淑芬、陳建源。2001。免疫感測器之技術發展及其應用。化學，59(2)：263-271。
— 26 —
林璧鳳
◎期刊論文
1. Wu, W.M., B.L. Chiang, S.C. Chang and B.F. Lin. 2001. Dietary fish oil increases
CD8+ T cells and decreases autoreactive T cells in autoimmune NZBxNZW F1 mice.
Journal of Microbiology, Immunology and Infection. 34: 41-49.
◎研討會論文
1. Lin, J.Y., M.L. Chen, B.L. Chiang and B.F. Lin. 2001. Ganoderma tsuga exerts
anti-inflammatory effect in animal model of airway inflammation. Annals of Nutrition
& Metabolism 45(s): 290.
2. Cheng, H.C. and B.F. Lin. 2001. The effect of different preparations of high frying oil
intake on immune responses in animal model of asthma Annals of Nutrition &
Metabolism 45(s): 290.
3. Hong, Y.H. and B.F. Lin. 2001. Establishment of in vitro model to study the effect of
health food on anti-inflammation. Annals of Nutrition & Metabolism. 45(s): 290.
4. Lin, J.Y., B.L. Chiang and B.F. Lin. 2001. Wild Amaranchus spinosus linn. Inhibits
spontaneous apoptosis of immune cells of BALB/c mice. Annals of Nutrition &
Metabolism 45(s): 293.
5. Hsieh, C.C. and B.F. Lin. 2001. Vitamin E supplement modulates the effect of
oxidizing frying oil diet and dietary fat content on disease course of NZB/W F1 mice.
Annals of Nutrition & Metabolism 45(s): 593.
潘子明
◎期刊論文
1. Luo, W.C. and T.M. Pan. 2001. Investigation of the antibiotic resistance of
Enterobacteriaceae of swines in Taiwan. Taiwanese J. Agri. Chem. & Food Sci. 39. (in
press)
2. Lin, K.C. and T.M. Pan. 2001. Screening of di-(2-ethylhexyl) phthalate-degrading
microorganism and the optimization of biodegradation conditions. Taiwanese J. Agri.
Chem. & Food Sci. 39. (in press)
3. Lin, J.S., J.L. Lee and T.M. Pan. 2001. Relationship analysis of the oubbreak of
shigellosis at Tau-Yuan area. Epidemiology Bulletin. 17: 168-177.
4. Lin, Y.L., C.C. Chou and T.M. Pan. 2001. Screening procedure from cattle feces and
— 27 —
the prevalence of Escherichia coli O157:H7 in Taiwan dairy cattle. J. Microbial
Immunol. Infect. 34: 17-24.
◎研討會論文
1. Pan, T.M., F.M. Lin and C.H. Chiu. 2001. Isolation of Lactobacillus paracasei subsp.
paracasei NTU101 strains for use in fermentation of milk-soymilk with Lycium
chinense Miller, Asian First Symposium on Lactic Acid Bacteria.
2. Pan, T.M. and F.M. Lin. 2001. Application of lactic acid bacteria isolated from Taiwan,
The 39th Annual Meeting of the Chinese Agricultural Chemical Society.
3. Pan, T.M. and J.Y. Tsai. 2001. Studies on the detection and molecular typing of
Escherichia coli O157:H7. The 39th Annual Meeting of the Chinese Agricultural
Chemical Society.
4. Pan, T.M. and E.R. Liou. 2001. Studies on the detection and molecular typing of
Salmonella enteritidis. The 39th Annual Meeting of the Chinese Agricultural Chemical
Society.
5. Pan, T.M. and Y.Y. Chia. 2001. Studies on the production of polyglutamate. Annual
Symposium of Chinese Institute of Food Science and Technology.
6. Pan, T.M. and J.W. Shi. 2001. Studies on the detection method of su-fu, miso and
chaw-tofu derived from GMO. Annual Symposium of Chinese Institute of Food
Science and Technology.
7. Pan, T.M. and T.Y. Tsai. 2001. Study on molecular typing of Escherichia coli O157:H7
The 35th Annual Meeting of the Chinese Society of Microbiology.
8. Pan, T.M. 2001. Current Development and Determination of Genetically Modified
Food, Symposium on GMO of GMP Association 1-21.
9. Pan, T.M. and K.C. Lin. 2001. Studies on the screening of bacteria and the
optimization of the degredation of DEHP, Symposium on Environmental Analytical
Chemistry 2001.
10. Pan, T.M. 2001. Infection of Escherichia coli O157:H7, Workshop of Infection
Disease by Center for Disease Control C12-C60.
11. Pan, T.M., L.M. Chen and Y.C. Su. 2001. Development of primers specific to hlyA,
eaeA, stx1, stx2, fliC and rfb genes for identification and characterization of
Enterohemorrhagic Escherichia coli O157:H7 by multiplex PCR, 101 General Meeting
of American Society for Microorganism 270.
12. Pan, T.M., L.M. Chen and T.Y. Tsai. 2001. Molecular typing of Escherichia coli
— 28 —
O157:H7 isolated in Taiwan by pulsed-field gel electrophoresis and random
amplification polymorphic DNA, 101 General Meeting of American Society for
Microorganism 260.
13. Pan, T.M. 2001. Detection of E. coli O157:H7 from food, animal and human,
Symposium on the Detection of Important Clinical Microorganisms 78-135.
14. Pan, T.M. 2001. Determination of GMO, Symposium on GMO 78-108.
◎專書
1. 潘子明。2001。機能性醱酵製品，藝軒圖書出版社。
2. 潘子明。2001。食品生物技術，藝軒圖書出版社。
許瑞祥
◎期刊論文
1. Wu, H.L., R.S. Hseu and L.P. Lin. 2001. Identification of Chlorella spp. isolates using
ribosomal DNA sequence. Botanical Bulletin of Academia Sinica 42: 115-121. (SCI)
◎專利
1. 「Method for identifying Cordyceps sinenis」獲得美國專利第 6,271,003 號(August 7,
2001)。
2. 「Gene sequence and method for distinguishing Cordyceps sinensis」獲得美國專利第
6,251,606 號(June 26, 2001)。
黃慶璨
◎期刊論文
1. Shih, P.C. and C.T. Huang. 2001. Effects of quorum sensing deficiency on the
Pseudomonas aeruginosa biofilm formation and resistance. Journal of Antimicrobial
Chemotherapy (in press，SCI)
2. Chen, C.C., C.T. Huang and K.J. Cheng. 2001. Improvement of phytase
thermostability
using
sorghum
liquor
waste
Biotechnology Letters 25: 331-333. (SCI)
楊健志
— 29 —
supplemented
with
starch.
◎ 期刊論文
1. Chen, P.Y., B.G. Gopalacushina, C.C. Yang, S.I. Chan and P.A. Evans. 2001. The role
of a -buldge in the folding of the –hairpin structure in ubiquitin. Protein Sci. 10:
2075-2082. (SCI)
李昆達
◎ 期刊論文
1. Lee, K.T., T. Hirano, T. Yamakawa, T. Kodama, Y. Igarashi and K. Shimomura. 2001.
Responses of transformed root culture of Atropa belladonna to salicylic acid. J. Biosci.
Bioeng. 91: 586-589. (SCI)
— 30 —
第六項

選擇性主要期刊論文摘要
Su, S.C. and C.Y. Lee. 2001. Cloning of the N-acylamino acid racemase gene from
Amycolatopsis azurea and biochemical characterization of the gene product.
Enzyme Microb. Technol. 30:647-655.(SCI)
Abstract:
A gene encoding N-acylamino acid racemase (NAAAR) from Amycolatopsis
azurea CCRC13413 was cloned. An analysis of its sequence revealed an open reading
frame encoding 368 amino acid residues. The deduced amino acid sequence of
NAAAR showed an 89% identity with that of Amycolatopsis sp. TS-1-60. The
N-acylamino acid racemase gene (aaar) was subcloned into expression vector pET-17b.
and was transformed into E. coli BL21 (DE3). A 41-kDa protein band was present on
the SDS-PAGE gel from the intracellular soluble part of E. coli. The recombinant
NAAAR produced in E. coli was purified by Toyopearl DEAE-650M and Sephacryl
S-200 h chromatography. The molecular weight of NAAAR, determined by
gel-filtration chromatography, was 320 kDa, indicating that NAAAR was composed of
eight identical subunits. NAAAR had maximal activity at 40degreesC and pH 7.4. The
enzyme activity was enhanced notably by Co2+ and Mn2+. Substrate specificity
revealed that N-acetyl-D-methionine. N-acetyl- L-methionine, N-acetyl-D-tyrosine,
N-acetyl-L-valine. and N-chloroacetyl -D-phenylalanine were the effective substrates,
For the substrate N- acetyl-D-methionine K-m was 27.8 mM with a V-max of 0.07
mumol(-1) min(-1) and a k(cat)/K-m of 311.4 M-1 s(-1). Our data revealed that this
N-acylamino acid racemase has a broad substrate range, In addition. we procured the
distribution of sequence-conserved regions from known NAAARs and putative
NAAARs in this study.

Lee, C.Y., M.F. Cheng, M.S. Yu and M.J. Pan. 2001. Purification and
characterization of a putative virulence factor, serine protease, from Vibrio
parahaemolyticus. FEMS Microbiol. Lett. 209:30-36.(SCI)
Abstract:
A protease (protease A) was successfully purified from the extracellular proteins
of Vibrio parahaemolyticus no. 93. a clinical strain carrying neither tdh nor trh genes,
using phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The
molecular mass of protease A was 43 kDa using gel Filtration, which was in agreement
with the results obtained from SIDS PAGE, suggesting that protease A was a
monomeric protein. Additionally, the isoelectric point of this protein was 5.0. The
optimum temperature and pH of protease A ranged from 40degreesC to 50degreesC
and pH 8, respectively. Protease A activity was inhibited by serine protease inhibitors.
— 31 —
such as phenylmethylsulfonyl fluoride and soybean trypsin inhibitor; moreover, the
activity could be blocked by treatment with 20 mM of 1,10-phenanthroline, but could
not be restored by adding metal ions. These results indicated that protense A is a serine
protease that requires metal. The 12 N-terminal residues Of protease A showed a high
degree of identity (81%) to the sequence of Vibrio metschnikovii VapT serine protease.
The purified protease had significant effects on the growth of Chinese hamster ovary,
HeLa, Vero and Caco-2 cells and its cytotoxic activity was not blocked by
gangliosides. Protease A lysed erythrocytes well but its hemolytic activity was
unstable after heat treatment, indicating that protease A is able to cause hemolysis but
is a heat-labile protein. The purified protease caused tissue hemorrhage and death in
mice when injected both intraperitoneally and intravenously. In conclusion, this is the
First report of a serine protease Purified directly from the supernatant of V
parahaemolyticus and identifying it as a potential virulence factor. (C) 2002
Federation of European Microbiological Societies.

Chen, I.M., F.C. Chang, M.F. Hsu and Y.S. Wang. 2001. Comparisons of PCBs
dechlorination occurences in various contaminated sediments. Chemosphere
43(7): 649-654. (SCI)
Abstract:
A comparison was made of reductive dechlorination occurrences of
polychlorinated biphenyls (PCBs) by microorganisms collected from contaminated
sediments including Er-Jen River (Tainan, Taiwan), Hudson River (Ft. Edward, NY),
Silver Lake (Pittsfield, MA) and Puget Sound (Washington State). Comparisons was
made in terms of chromatographic data (referring to the biological activity, including
microbial availability) and thermodynamic data (demonstrating the selectivity of
anaerobic microorganisms in the dechlorination of chlorinated compounds).
Chromatographic data was established in terms of difference in relative retention time
(Delta ln RRT) and thermodynamic data was estimated as heat of reaction
(DeltaH(r)(0)). Both were calculated and correlated to occurrences of dechlorination
reactions. Observed dechlorination reactions for individually introducing PCB
congener had Delta ln RRT levels measured as >0.47 (Er-Jen River), >0.29 (Hudson
River), >0.36 (Silver Lake) and >0.45 (Puget Sound, for Aroclor 1254 dechlorination).
Critical of DeltaH(r)(0) and Delta ln RRT values showed that Hudson River and Silver
Lake microorganisms were capable of dechlorinating PCBs through reactions with
larger H-r(0) value (lower levels of released energy) and smaller Delta ln RRT value
compared with those found in Er-Jen River and Puget Sound sediments. Differences in
the critical Delta ln RRT values of these sediments may be due to differences in their
levels of PCB contamination.

Chen, I.M., F.C. Chang and Y.S. Wang. 2001. Correlation of gas chromatographic
— 32 —
properties of chlorobenzenes and polychlorinated biphenyls with the occurrence
of reductive dechlorination by untamed microorganisms. Chemosphere 45(2):
223-229. (SCI)
Abstract:
To understand the dechlorination ability of chlorobenzenes (CBs) and
polychlorinated biphenyls (PCBs) by untamed microorganisms under anaerobic
condition and to correlate gas chromatographic properties with the occurrence of
reductive dechlorination, introduction of CBs and PCBs in the culture medium
inoculated with microorganisms from sludge and sediment, respectively, were
performed. Three kinds of culture media preparing from sludge, river water and a
synthetic medium were used in the experiments. HCB was degraded to
1,3,5-trichlorobenzene (1,3,5-TCB) and 1,3-dichlorobenzene (1,3-DCB) in both
sludge medium and synthetic medium with inoculated microorganisms. Three PCB
congeners including 2,3,4-, 3,4,5- and 2,3,4,5-CBp (chlorinated biphenyl) were not
found to be dechlorinated in the river water medium with inoculation culture but to be
dechlorinated in the synthetic medium. MNDO methodology was used to compute
theoretical dechlorination reaction heats and GC-ECD techniques were used to
estimate chromatographic data of CB and PCB congeners. Both CB and PCB
congeners showed that dechlorination by untamed microorganisms under anaerobic
mixed cultures were more likely to occur when larger amounts of energy were released
and greater Delta ln RRT value between the parent congener and the daughter product
was observed. Delta ln RRT provided a more precise information on the singularity of
PCBs ortho-dechlorination in an aspect of thermodynamic favorable rule.

Yen, J.H., C.C. Tsai, C.C. Su and Y.S. Wang. 2001. Environmental dissipation of
fungicide triphenyltin acetate and its potential as a groundwater contaminatant.
Ecotoxicol. Environ. Safe. 49: 164-170. (SCI)
Abstract:
The effects of various factors on fungicide triphenyltin acetate dissipation in
selected soil types were studied; temperature is clearly the most important factor of
these. Residues of this fungicide in Fangtzupo clay loam and Lanyang loam incubated
at 30 degreesC for 32 days (water content 100, 70, or 40% of field capacity) were 25%
of the original amounts. In soil samples incubated at 10, 30, or 40 degreesC with
100% field capacity water content, residues were 80, 26, and 5.5% for Fangtzupo soil,
respectively, and 83, 26, and 7.0% for Lanyang soil, respectively. No significant
difference in dissipation rate was found between sterilized and unsterilized soil,
suggesting that microbial degradation is not an important factor in this process. Rapid
degradation rates were observed-half-lives of 8.3 to 19.4 days in Fangtzupo clay loam
and 8.0 to 16.3 days in Lanyang loam at temperatures ranging from 30 to 40 degreesC
(soil water content of 100, 70, and 40% of field capacity). Significant increases in
— 33 —
half-life (approximately 150 days) occurred at lower temperatures (10 degreesC) in
both soil types. The potential of triphenyltin acetate to contaminate groundwater was
tested using the behavior assessment and groundwater pollution-potential models.
Results indicate that the leaching rate of the fungicide is very slow (1.0 cm day(-1))
under normal conditions.

Chen, I.M., B.V. Chang, S.Y. Yuan and Y.S. Wang. 2001. Reductive
dechlorinatioon of hexachlorobenzene under various additions. Water, Air, and
Soil Pollution 139 (1-4): 61-74(SCI)
Abstract:
Microorganisms collected from sediments of Ho-Tsin River in southern Taiwan were
used in this study. The ability to dechlorinate hexachlorobenzene (HCB) was induced by
enrichment incubation in yeast extract amended culture and acclimation with HCB.
Addition of lactate or replacement of yeast extract by lactate did not enhance the
dechlorination ability. With strong electron capturing capability, denitrifying bacteria
resulted in complete inhibition of dechlorination in the mixed culture contained nitrate. In
the culture amended with sulfate, sulfate reducing bateria shared electrons and nutrient
with HCB-dechlorinating consortium but grabbed more electrons when treated with
vancomycin. Results from multi-factors tests indicate that the influences of factors on
dechlorination were complicated. Dechlorinating microbes, electron suppliers, sulfate
reducing bacteria and denitrifying bacteria, all possibly caused a great effect on
dechlorination.

Su, N.W. and M.H. Lee. 2001. Purification and characterization of a Novel
Salt-tolerant Protease from Aspergillus sp. FC-10, a soy sauce koji mold. Journal
of Industrial Microbiology and Biotechnology 26: 253-258. (SCI)
Abstract:
A novel salt-tolerant protease produced by Aspergillus sp. FC-10 was purified to
homogeneity through anionexchange chromatography, preparative isoelectric
-focusing electrophoresis, and gel filtration chromatography, with an overall recovery
of 12.7%. This protease demonstrated an optimum pH range of 7.0-9.0 for activity,
with a stable pH range of 5.0-9.0. The optimum process temperature at pH 7.0 was
65degreesC. The enzyme has a molecular mass of 28 kDa and was deduced as a
monomer with an isoelectric point of 3.75. Enzyme activity was strongly inhibited by
5 mM of HgCl2 and FeCl3, and significantly inhibited by 5 MM Of CUSO4, FeSO4,
and MnCl2. The activity of this purified protease was inhibited by Na-2.EDTA;
however, leupeptin, pepstatin A, PMSF, and E-64 did not affect the activity. Based on
the N-terminal amino acid sequence and amino acid composition, this purified
protease should be classified as a member of the deuterolysin family
— 34 —

Su, N.W. and M.H. Lee. 2001. Screening and characterization of koji molds
producing saline-tolerant protease. Journal of Industrial Microbiology and
Biotechnology 26: 230-234. (SCI)
Abstract:
Three mold strains isolated from soil in the Taipei area of Taiwan were compared
with a commercial strain of Aspergillus oryzae for their proteolytic activities in an
18% NaCl aqueous solution system. Among these strains, the one subsequently
identified and designated as Aspergillus sp, FC-10 produced protease with superior
saline tolerance. In aflatoxin tests, this strain did not generate detectable aflatoxin after
growing on steamed grain polished rice substrate for 24 days. Two types of
extracellular proteases were preliminary fractionated by column chromatography on
DEAE Sepharose CL-6B. Proteolytic activity of the nonadsorbed protease (P-I) was
reduced to 9.4% in the 18% NaCl solution compared to its original activity determined
in the buffer solution, However, the adsorbed protease (P-II) was particularly salt
tolerant and stable, with 50% proteolytic activity retained throughout the 6-h stability
test in 18% NaCl solution.

Hseu, Z.Y. and Z.S. Chen. 2001. Quantifying soil hydromorphology of a
rice-growing Ultisol toposequence in Taiwan. Soil Sci. Soc. Am. J. 65 (1): 270-278.
(SCI)
Abstract:
Seasonally irrigated flooding and fluctuation of groundwater level control the
redoximorphic features, saturation, and reduction of rice-growing Ultisols in different
landscape positions of Taiwan, The objective of this study was to quantify soil
hydromorphology of an Ultisol toposequence with different saturation and reduction
conditions. Three study soils, along a toposequence at the red earth terrace, were
selected for monitoring of the water table, matric potential, and redox potential (Eh) at
various soil depths in 1996 and 1997, The three soils are Houhu (Typic Plinthaquult)
in the toeslope, Hsinwu (Typic Plinthaquult) in the footslope, and Lungchung
(Plinthaquic Paleudult) in the lower backslope, The Houhu and Lungchung soils were
seasonally flooded for rice (Oryza sativa L.) production and produced perched water
tables from March to October, The Hsinwu soil, where rice has not been planted since
1991, still revealed seasonally high water levels and perched water tables during the
study period. Redox concentrations originally occurred as soft masses and concrete
nodules associated with seasonally high water levels, but irrigation and drainage
processes also influenced the development of redoximorphic features. The abundance
of Fe-Mn concretions and Fe depletions increased as cycling of oxidation and
reduction conditions increased in rice production. The durations of saturation and
reduction in the Btv horizons of the Houhu soil in the toeslope position were more
than 80% of the year, and the soil had approximate to 10% of Fe-Mn concretions. The
— 35 —
Btv horizons of the Hsinwu soil in the footslope position were saturated for 50% of the
year and reduced for 25% of the year, and the soil had approximate to 20% Fe-Mn
concretions. The Btv horizons of the Lungchung soil in the lower backslope position
were saturated for 40% of the year and reduced for only approximate to 10% of the
year, and the soil had 15% Fe-Mn concretions, The Houhu and Hsinwu soils had
anthraquic conditions and the Lungchung soil, with less reduction, was proposed as
having oxyaquic conditions as defined in U,S, soil taxonomy.

Hseu, Z.Y. and Z.S. Chen. 2001. Quantifying soil hydromorphology of a
rice-growing Ultisol toposequence in Taiwan. Soil Sci. Soc. Am. J. 65(1): 270-278.
(SCI)
Abstract:
Seasonally irrigated flooding and fluctuation of groundwater level control the
redoximorphic features, saturation, and reduction of rice-growing Ultisols in different
landscape positions of Taiwan, The objective of this study was to quantify soil
hydromorphology of an Ultisol toposequence with different saturation and reduction
conditions. Three study soils, along a toposequence at the red earth terrace, were
selected for monitoring of the water table, matric potential, and redox potential (Eh) at
various soil depths in 1996 and 1997, The three soils are Houhu (Typic Plinthaquult)
in the toeslope, Hsinwu (Typic Plinthaquult) in the footslope, and Lungchung
(Plinthaquic Paleudult) in the lower backslope, The Houhu and Lungchung soils were
seasonally flooded for rice (Oryza sativa L.) production and produced perched water
tables from March to October, The Hsinwu soil, where rice has not been planted since
1991, still revealed seasonally high water levels and perched water tables during the
study period. Redox concentrations originally occurred as soft masses and concrete
nodules associated with seasonally high water levels, but irrigation and drainage
processes also influenced the development of redoximorphic features. The abundance
of Fe-Mn concretions and Fe depletions increased as cycling of oxidation and
reduction conditions increased in rice production. The durations of saturation and
reduction in the Btv horizons of the Houhu soil in the toeslope position were more
than 80% of the year, and the soil had approximate to 10% of Fe-Mn concretions. The
Btv horizons of the Hsinwu soil in the footslope position were saturated for 50% of the
year and reduced for 25% of the year, and the soil had approximate to 20% Fe-Mn
concretions. The Btv horizons of the Lungchung soil in the lower backslope position
were saturated for 40% of the year and reduced for only approximate to 10% of the
year, and the soil had 15% Fe-Mn concretions, The Houhu and Hsinwu soils had
anthraquic conditions and the Lungchung soil, with less reduction, was proposed as
having oxyaquic conditions as defined in U,S, soil taxonomy.

Chen, Z.S., T.C. Tsou, V.B. Asio and C.C. Tsai. 2001. Genesis of Inceptisols on a
— 36 —
volcanic landscape in Taiwan. Soil Sci. 166(4): 255-266. (SCI)
Abstract:
Very little data are available regarding the genesis of Inceptisols derived from pyroclastic
materials in Taiwan because most of the earlier research focused on Andisols. This study
was conducted to explain the formation of Inceptisols derived from the pyroclastic
material in northern Taiwan. Three pedons representing the summit, backslope, and
footslope positions of a volcanic landscape were examined and sampled for physical,
chemical, and mineralogical analyses. Results revealed that the soils were moderately
developed and characterized by an A-BA-Bw-BC horizon sequence, loam to clay loam
texture, firm and subangular blocky structure, low bulk density, high water, and P
retention. The soils were very acid, with low exchangeable bases but very high
exchangeable Al as well as considerable amounts of Al associated with humus. Gibbsite
and quartz were found to be dominant in the sand fraction, whereas gibbsite, kaolinite,
and 2:1. minerals were the major minerals in the clay fraction. Formation of the soils was
relatively fast because of the easy weatherability of the pyroclastic materials, perhumid
climate, and good drainage of the volcanic landscape. Since these Inceptisols possessed
several Andic soil features, we hypothesize that they probably developed from earlier
formed Andisols. Therefore, it is likely that the sequence of soil formation was Entisol
--> Andisol --> Inceptisol. The most important soil-forming processes in the formation of
the Inceptisols were likely structure formation, loss of bases and acidification,
braunification, bioturbation, organic matter accumulation, weathering, and clay mineral
formation.

Chen, M.C., M.K. Wang, C.Y. Chiu, P.M. Huang and H.B. King. 2001.
Determination of low molecular weight dicarboxylic acids and organic functional
groups in rhizosphere and bulk soils in a temperate forest soils. Plant and Soil. 31:
37- 44. (SCI)
Abstract:
Low molecular weight organic acids (LMWOAs) derived from root exudates,
decomposing organic matter, and other sources are important ligands. The species of
these LMWOAs in the Tsuga rhizosphere soil (TRS), and Yushania rhizosphere soil
(YRS), and bulk soil (BS) from an alpine forest region were identified. LMWOA and
organic functional groups were used to those fresh twigs and leaves, litters, and roots
as comparison. The objectives of this study were to (i) develop a method that could be
used to determine LMWOAs in soil solution by gas chromatography (GC), (ii) assess
methods for processing LMWOAs in soil samples, and (iii) determine the relative
proportions of organic carbon functional groups in the TRS, YRS and BS, and fresh
plant materials with C-13 nuclear magnetic resonance (C-13 NMR) analysis. The
proportion of organic acid contents followed the order of YRS > TRS > BS, and also
showed significant differences (P < 0.05) from GC analysis. The amounts of malonic,
— 37 —
fumaric and succinic acids in the YRS samples were greater than in the TRS and BS.
Samples analyzed after 1 month of deep freeze storage (-24 degreesC) showed no
signs of decomposition. The proportion of organic functional groups in the rhizosphere
and bulk soils quantified by C-13 NMR analyses followed the general order: alkyl-C >
O-alkyl-C > N-alkyl-C > acetal-C > aromatic-C > carboxylic-C > phenolic-C.

Juang, T.C., M.K. Wang, H.C. Chen and C.C. Tan. 2001. Ammonium fixation by
surface soils and clays. Soil Sci. 166: 345-356. (SCI)
Abstract:
Ammonium fixation by soil clays may influence N availability to plants; however,
the nature of the clay minerals responsible for NH4-fixation is unclear. The first
objective of this study was to investigate how ammonium concentration and
temperature affect ammonium fixation. The second objective was to determine
whether NH4-fixation is caused by tetrahedrally or octahedrally charged 2:1
phyllosilicates. Twelve surface (0-30 cm) soils were collected in Taiwan, An alkaline
potassium hypobromite (KOBr-KOH) solution was employed to remove exchangeable
and organic NH4+, and a 5 M HF 1 M HCl solution was used to extract fixed NH4
from the soil clays. The results indicated that the amount of NH4 fixed by soils and
clays increased with temperature and NH4 concentration. Characterization of soil
clays by X-ray diffraction (XRD) with K- and Mg-saturated clays indicated the
presence of montmorillonite, illite, kaolinite, and vermiculite. X-ray diffraction
analysis of dodecylammonium-saturated samples indicated a mean layer charge of
0.40-0.56 e/O-10(OH)(2) for the montmorillonite, Heating LiCl-saturated clays at 300
degreesC neutralized the octahedral layer charge. The correlation between fixed
ammonium and the octahedral layer charge NH4+ fixation (R-2 = 0.86) suggests a
possible explanation for the fixed ammonium.

Chang, C.M., M.K. Wang, T.W. Chang and C. Lin. 2001. Transport modeling of
copper and cadmium with linear and nonlinear retardation factors. Chemosphere
43: 1133-1139. (SCI)
Abstract:
The predictive accuracy of using the one-dimensional advection-dispersion
equation to evaluate the fate and transport of solute in a soil column is usually
dependent on the proper determination of chemical retardation factors. Typically, the
distribution coefficient (K-d) obtained by fitting the linear sorption isotherm has been
extensively used to consider general geochemical reactions on solute transport in a
low-concentration range. However, the linear distribution coefficient cannot be
adequately utilized to describe the solute fate at a higher concentration level. This
study employed the nonlinear equilibrium-controlled sorption parameters to determine
the retardation factor used in column leaching experiments. Copper and cadmium
— 38 —
transportation in a lateritic silty-clay soil column was examined. Through the explicit
finite-difference calculations with a third-order total-variation-diminishing (TVD)
numerical solution scheme, all results of the theoretical copper and cadmium
breakthrough curves (BTCs) simulated by using the Freundlich nonlinear retardation
factors revealed good agreement with the experimental observations.

Yang, D.S. and M.K. Wang. 2001. Characterization and a fast method to
synthesis of sub-micron lithiophorite. Chemical of Materials 13: 2589-2594. (SCI)
Abstract:
A modified oxidation-deprotonation reaction (ODPR) was developed to
synthesize well-crystallized birnessite. We compared the conventional methods for
synthesis of birnessite with the ODPR approach. The structural chemistry of birnessite
was evaluated by high-resolution transmission electron microscopy (HRTEM), using
an energy-dispersive spectrometer (EDS) to determine the Na+ content. The average
oxidation states (AOS) of birnessite were determined by potentiometric titration.
Infrared spectrometric (IR) and X-ray diffraction (XRD) analytical techniques were
used to characterize birnessite. The HRTEM lattice image and low Na+ content
suggest that there are no vacancies in the octahedral layers of birnessite. The AOS
values of birnessite ranged from 3.77 to 3.87 and the presence of IR bands at 1153 and
1084 cm(-1) were attributed to the Mn3+-OH deformation. The results show evidence
for the presence of the Mn3+ or Mn2+ in the structure of birnessite.

Chen, P.Y., M.K. Wang and D.S. Yang. 2001. Mineralogy of dickite and nacrite
from northern Taiwan. Clays and Clay Minerals 49,(6):586-595. (SCI)
Abstract:
A modified oxidation-deprotonation reaction (ODPR) was developed to
synthesize well-crystallized birnessite. We compared the conventional methods for
synthesis of birnessite with the ODPR approach. The structural chemistry of birnessite
was evaluated by high-resolution transmission electron microscopy (HRTEM), using
an energy-dispersive spectrometer (EDS) to determine the Na+ content. The average
oxidation states (AOS) of birnessite were determined by potentiometric titration.
Infrared spectrometric (IR) and X-ray diffraction (XRD) analytical techniques were
used to characterize birnessite. The HRTEM lattice image and low Na+ content
suggest that there are no vacancies in the octahedral layers of birnessite. The AOS
values of birnessite ranged from 3.77 to 3.87 and the presence of IR bands at 1153 and
1084 cm(-1) were attributed to the Mn3+-OH deformation. The results show evidence
for the presence of the Mn3+ or Mn2+ in the structure of birnessite.

Liu, C.L., M.K. Wang and C.C. Yang. 2001. Determination of cation-exchange
capacity by one-step soil leaching column method. Comm. Soil Sci. and Plant
— 39 —
Analy. 32(15&16): 2359-2372. (SCI)
Abstract:
Many methods have been proposed for measuring exchangeable cations and
cation exchange capacity (CEC) in soils. Most of these methods are multi-step
operations, which are time-consuming and, therefore, not applicable for routine soil
tests. Speed and simplicity of operations are necessary. The objective of this study was
to develop a one-step soil column leaching method to determine CEC using 0.01 M
SrCl2 solution [CEC(SrCl2)]. Measured CEC(SrCl2) values correlate to the
conventional CEC [I M NH4-acetate (NH4OAc, pH 7)] in slightly acidic, alkaline, and
neutral pH soils. CEC(SrCl2)was also compared to the effective CEC (ECEC by 0.01
M SrCl2 and pH 7, 1 M NH4OAc solutions (soil column leaching method) in red soils.
Here, ECEC(SrCl2) and ECEC(NH4OAc) are the sum of exchangeable K+, Na+,
Ca2+, Mg2+, and Al3+ in soil column leachates. The correlation between CEC(SrCl2)
versus ECEC(SrCl2), and CEC(SrCl2) versus ECEC(NH4OAc) of seven red soils
showed a linear relationship (r(2) values being 0.99 and 0.98, respectively). The
correlation between CEC(SrCl2) and conventional CEC(NH4OAc) of five soils (i.e.,
slightly acidic, alkaline or neutral pH soils) was 0.99. However, a comparison of
CEC(SrCl2) to conventional CEC(NF4OAc) shows significant differences (p < 0.01)
for seven red soils. but not for five other soils (p > 0.05). Thus, these results indicate
that the advantage of the CEC(SrCl2) from one-step soil leaching column technique
can consider it as ECEC of red soils and CEC of the other slightly acidic, alkaline and
neutral pH soils. The one-step soil leaching column method is a rapid, simple
technique for determining CEC of most soils and ECEC of red soils.

Chiu, C.Y., M.K. Wang, H.B. King and J.L. Hwong. 2001. Physical and chemical
properties of rhizosphere and bulk soils of Tsuga and Yushania in a temperate
forest soils. Commun. Soil Sci. Plant Anal. 33 (11-12): 1723-1735. (SCI)
Abstract:
The objectives of this study were to investigate selected physical and chemical
properties, and availability of metals in rhizosphere and bulk soils of Tsuga and
Yushania of alpine forests. The pH of rhizosphere soils was lower than bulk soils.
Mean CEC, organic carbon, carbon/nitrogen (C/N) ratio, and clay contents in
rhizosphere soils were greater than in bulk soils (P < 0.05). Concentrations of
exchangeable potassium (K) and magnesium (Mg) were also greater in rhizosphere
soils than in bulk soils. The availability of aluminum (Al), iron (Fe), manganese (Mn),
copper (Cu), and zinc (Zn) in rhizospheres was influenced by the ionic species and
concentrations, which depend on the pH change and chemical compositions of root
exudates.

Chang, T.W., M.K. Wang and C. Lin. 2001. Adsorption in the different
— 40 —
sorbent/water ratios of soil systems. Water, Air, and Soil Pollution. 138 (1-4):
199-209. ( SCI)
Abstract:
While sorbate/sorbent, sorbate/water, and sorbent/water (S/W) ratios in a batch system
are known to affect the adsorption of sorbate, the effect of different S/W ratios on the
adsorption of inorganic sorbates has seldom been addressed. This study investigates the
adsorption phenomena of Cu2+ in different sorbate/sorbent/water ratios in a batch
system. Batch experiments are performed to examine the adsorption, and the linear
(K-D), Langmuir (K-L), and Freundlich (K-F) adsorption coefficients of Cu2+ in
lateritic soil. These experiments are conducted using solutions with initial
concentrations of 0.5 to 50 mg Cu2+ L-1 equilibrated with an appropriate amount of
soil to give S/W ratios of 0.1 to 2 g mL(-1). Although the variations in the sorbed
amount and adsorption coefficients apparently originate from a change in the
sorbate/sorbent/water ratio, only the equilibrium concentration significantly affects
adsorption. On the other hand, the linear and Langmuir isotherm cannot adequately
describe the adsorption data. In this study, the Freundlich equation gave an excellent fit
to the adsorption data with a goodness-of-fit (R-2) > 0.984. However, adsorption
isotherms should be regarded as only a curve-fitting model or a mathematical tool and
cannot be employed to interpret any particular adsorption mechanism. Meanwhile, the
solids effect reveals that K-F and maximum adsorption (b) of the Langmuir equation
increase when S/W ratio decreases. The sorbate in the stagnant phase must be
considered as part of the equilibrium concentration in the solid phase to avoid
underestimating the sorbed amount at a lower S/W ratio and/or a higher sorbate
concentration level.

Juang, K.W., D.Y. Lee and T.R. Ellsworth. 2001. Using rank order geostatistics
for spatial interpolation of highly skewed data in a heavy metal contaminated site.
J. Environ. Qual. 30: 894-903. (SCI)
Abstract:
The spatial distribution of a pollutant in contaminated soils is usually highly
skewed. As a result, the sample variogram often differs considerably from its regional
counterpart and the geostatistical interpolation is hindered. In this study, rank-order
geostatistics with standardized rank transformation was used for the spatial
interpolation of pollutants with a highly skewed distribution in contaminated soils
when commonly used nonlinear methods, such as logarithmic and normal-scored
transformations, are not suitable. A real data set of soil Cd concentrations with great
variation and high skewness in a contaminated site of Taiwan was used for illustration.
The spatial dependence of ranks transformed from Cd concentrations was identified
and kriging estimation was readily performed in the standardized-rank space. The
estimated standardized rank was back-transformed into the concentration space using
— 41 —
the middle point model within a standardized-rank interval of the empirical
distribution function (EDF). The spatial distribution of Cd concentrations was then
obtained. The probability of Cd concentration being higher than a given cutoff value
also can be estimated by using the estimated distribution of standardized ranks. The
contour maps of Cd concentrations and the probabilities of Cd concentrations being
higher than the cutoff value can be simultaneously used for delineation of hazardous
areas of contaminated soils.

Lai, H.M. 2001. Effects of hydrothermal treatment on the physicochemical
properties of pregelatinized rice flour. Food Chemistry 72: 455-463. (SCI)
Abstract:
Effects of hydrothermal treatment on the pasting, thermal and hydration properties
of rice flour prepared from three Indica varieties of milled rice [Taichung Sen
Glutinous (TCSW) 1, Taichung Sen (TCS) 10, and TCS 17], which contained 1.2, 17.9
and 28.8% amylose, were studied. Three hydrothermally-treated factors, soaking time,
steaming temperature and steaming time were investigated. From the statistical results
of an experimental design of L-27 orthogonal array, the soaking time is a significant
factor that affects the pasting properties of three varieties of rice with various degrees
of influence. However, the increase of pasting temperature, reduction or. peak
viscosity and elevation of final viscosity were investigated for all treated non-waxy
rice and resulted in the reduction of its breakdown and total setback. The differences
of pasting and hydration properties of treated rice flour were attributed to the changes
of rigidity of starch granules under treatment. The results of DSC showed that the
degree of gelatinization was higher in waxy rice than in non-waxy rice when subjected
to the same hydrothermal conditions.

Wu, H.L., R.S. Hseu and L.P. Lin. 2001. Utilization of ribosomal DNA sequences
to identify Chlorella spp. isolates. Bot. Bull. Acad. Sin. 42:115-121. (SCI)
Abstract:
Members of the Chlorella species are very simple unicellular algae, easy to
cultivate and widely used in various physiological studies, Their morphological and
physiological characteristics, however, normally change with the environment, making
species identification difficult. TO elucidate the relationship between various strains of
Chlorella, this investigation analyzed the nuclear-encoded and chloroplast-encoded
small-subunit rDNA sequences of four strains of Chlorella using PCR techniques.
These strains were isolated from different rivers and ponds in Taiwan and Indonesia,
and then compared and identified using stock strains of Chlorella spp. from the culture
collection centers, and published DNA sequence data from Genbank. Experimental
results attributed the isolated strains mainly to C. sorokiniana, a common species of
green algae which grows in freshwater ecosystems at around 36 degreesC. In addition,
— 42 —
phylogenetic analysis of nuclear-encoded and chloroplast-encoded small-subunit
rDNA sequences from spherical green algae of the genera Chlorella revealed the
sequences to closely resemble each other. Further analyses indicated that Chlorella spp.
216 was dose to Chlorella spp. 21 and I. Generally, the chloroplast data sets supported
the lineages more than the nuclear data sets did. Strains 21 and 216 were closer to I.
Comparisons with some of the morphological and biochemical data indicated that the
phylogenetic analysis of rDNA sequences was in line with results obtained by
conventional methods.

Liu, C.P. and L.P. Lin. 2001. Ultrastructural study and DHA production of
Isochrysis sp. CCMP1324. Bot. Bull. Acad. Sin. 42: 207-214. (SCI)
Abstract:
This study investigates methods for extracting lipids from microalgae and
analyzes the effects of culture media as well as culture conditions on PUFA yields and
total fatty acid contents. Experimental results of an optimal culturing of Isochtysis spp.
were based on a 3.2% salinity culture medium. These microalgae were cultured in a
1-2 L Roux's flat-flask and a 5 L jar fermentor. The optimum culture temperature and
initial pH for DHA production were 25 degreesC and 8.0, respectively. Pigments
included chlorophylls a and c. The DHA yield increased with cultivation time until the
eighth day. Optimum DHA amounts in the cells were reached under aeration with 10%
CO2 and with continuous illumination of 10 klux. The biomass dry weight reached 4 g
per liter of culture, and the DHA production reached 16 mg per liter of culture. Lipid
bodies in Isochrysis spp. and related genera were observed during culture by light and
transmission electron microscopy; 0.5 similar to3.0 pm sized lipid bodies were
confirmed by staining with Sudan Black B in cells from log stage to stationary stage
cultures. These results demonstrated that DHA-containing lipid bodies in cells can be
produced and accumulated in marine Isochrysis spp.

Yang, S.S. and H.L. Chang. 2001. Methane emission from paddy fields in Taiwan.
Biology and Fertility of Soils 33: 157-165. (SCI)
Abstract:
In order to investigate the effect of environmental conditions on CH4 emission
from paddy fields in Taiwan, four locations, two cropping seasons and two irrigation
systems were studied. CH4 emission was high at the active tillering and the booting
stages in the first cropping season, whereas it was low at the transplanting and the
ripening stages with an intermittent irrigation system. CH4 emission was high at the
transplanting stage in the second cropping season, and decreased gradually during rice
cultivation. Daily temperature and light intensity increased gradually during rice
growth in the first cropping season (February-June), while it was reversed in the
second cropping season (August-December). The seasonal CH4 emission from paddy
— 43 —
fields ranged from 1.73 to 11.70 g m(-2), and from 10.54 to 39.50 g m(-2) in the first
and second cropping seasons, respectively. The seasonal CH4 emission in the second
cropping season was higher than that in the first cropping season in all test fields. The
seasonal CH4 emission was 32.65 mg m(-2) in the first cropping season of the
National Taiwan University paddy field with continuous flooding, and it was 28.85 mg
m(-2) in the second cropping season. The annual CH4 emission ranged from 12.3 to
49.3 g m(-2) with an intermittent irrigation system, and the value was 61.5 g m(-2)
with a continuous flooding treatment. The annual CH4 emission from paddy fields
was estimated to be 0.034 Tg in 1997 from 364,212 ha of paddy fields with an
intermittent irrigation system, which was less than the 0.241 Tg calculated by the
IPCC method with a continuous flooding treatment.

Yang, S.S. and C.M. Lei. 2001. Effect of culture media on protease and
oxytetracycline production in Streptomyces rimosus. World Journal of
Microbiology & Biotechnology 17: 403-410. (SCI)
Abstract:
For the simultaneous production of protease and oxytetracycline, mycelium and
protoplasts of Streptomyces rimosus TM-55 were cultivated in basal medium
containing soluble starch, corn steep liquid, ammonium sulphate, calcium carbonate,
sodium chloride and soybean oil. Protease and oxytetracycline production increased
with decreasing in ratio of culture broth to vessel volume from 1:2 to 1:5. Each ml of
broth with 0.286 mg fresh mycelia yielded 168-204 units of protease and 785-972 mug
of oxytetracycline after replacement of corn steep liquor, sodium chloride and soybean
oil with beef extract and sunflower oil, while each ml of broth with 7.5 x 10(7)
protoplasts produced 141-153 units of protease and 504-615 mug of oxytetracycline.
Protease and oxytetracycline production were low when the pH was less than or equal
to5.1 or greater than or equal to9.0. Soluble starch and ammonium sulphate were the
best carbon and nitrogen sources, respectively. Supplementation with calcium
carbonate enhanced protease and oxytetracycline production. The productivity of
protoplasts decreased sharply when the incubation temperature increased from 28 to
34 degreesC, while the productivity of mycelium was almost unchanged.

Yang, C.K. and S.S. Yang. 2001. Microbial ecology of soils surrounding nuclear
and thermal power plants in Taiwan. Environment International 26:
315-322.(SCI)
Abstract:
This paper reports a study of the effect of three nuclear and one thermal power
plants on the microbial ecology of soils. Populations of bacteria, actinomycetes, fungi,
cellulolytic microbes, phosphate-solubilizing microbes and nitrogen-fixing microbes
in the soil in the vicinity of each plant were studied. Soils were acidic at three sites,
— 44 —
and moisture contents of the power plant soils were lower than those of the
surrounding areas, Microbial populations of the topsoils (0-20 cm deep) were higher
than the subsoils (21-40 cm deep), and only 10-15% of them showed significant
difference (P < .05). Thirty-three percent of the samples from the surrounding areas
had higher microbial population than those from the power plant areas, but 19% was
the reverse. Populations of cellulolytic, phosphate-solubilizing and nitrogen-fixing
microbes varied with sampling locations, season and environmental conditions. Ratios
of cellulolytic, phosphate-solubilizing and nitrogen-fixing microbes to total viable
counts in some samples of the surrounding areas were significantly higher than in the
power plant areas. Although the microbial populations of power plant soil and its
surrounding area were somewhat different, it cannot be attributed as an effect of power
plant operation. as the differences were not consistent.

Chen, W.L., D.J. Hung, H.L. Wang, J.C. Su and P.D. Lee. 2001. Purification and
characterization of sucrose phosphase synthase from sweet potato tuber roots.
Bot. Bull. Acad. Sin. 42 (2): 123-129.(SCI)
Abstract:
Sucrose phosphate synthase (SPS) is one of the key enzymes in the sucrose
biosynthesis pathway. SPS was purified 40 fold from crude extract of sweet potato
tuberous roots by the methods of batch elution from DEAE-Sephacel, PEG
precipitation, omega -aminohexyl Sepharose 4B affinity and Mono Q union exchange
chomatographies. The native- and SDS-PAGE analyses revealed SPS to have a native
molecular mass of about 540 kDa, and it may therefore be homotetramer composed of
subunit with a mass of 130-140 kDa. The isoelectric point of the purified enzyme as
determined by IEF was 5.29. SPS fi-om the sweet potato tuberous root, which differs
from the SPS of photosynthetic tissues, was not allosterically regulated by G6P and Pi.
The Km for F6P and UDPG was 5.3 and 31.3 mM, respectively. The enzyme was
activated by Mn2+, Mg2+, and Ca2+, while being inhibited by Hg2+ The nucleotides
AMP, ADP, ATP, UMP, UDP, UTP, and TDP inhibited the enzyme about 30 similar to
50%. The enzyme was sensitive to sulfydryl reagents, but activity could be restored
with DTT or beta -ME. The enzyme was activated by glucose, glucosamine, maltose,
and lactose, but was inhibited by delta -gluconolactone. SPS could also be inhibited by
PCMBS and Cibacron blue F3G-A.

Chao, P.M., C.Y. Chao, F.J. Lin and C.J. Huang. 2001. Oxidized frying oil
up-regulates hepatic acyl coa oxidase and cytochrome P-450 4 A1 genes in rats
and activate PPAR-. J. Nutr. 131: 3166-3174. (SCI)
Abstract:
Oxidized LDL (oxLDL) and its component hydroxy fatty acids were shown to
activate peroxisome proliferator-activating receptor alpha (PPAR alpha) and gamma
— 45 —
(PPAR gamma). To test the hypothesis that lipid oxidation products in oxidized frying
oil (OFO) can activate PPAR alpha and up-regulate its target genes, a feeding
experiment and a transactivation experiment were conducted. Based on a 2 x 2
factorial design, four groups of Sprague-Dawley male weanling rats were fed diets
containing either high (20 g/100 g, HO and HF) or low (5 g/100 g, LO and LF) levels
of oxidized frying soybean oil (HO and LO) or fresh soybean oil (HF and LF) for 6 wk.
The OFO sample was prepared by frying wheat dough sheets in soybean oil at 205 +/5 degreesC for 24 h. OFO dose dependently and significantly increased (P < 0.05)
mRNA of acyl-CoA oxidase (ACO) and cytochrome P-450 4A1(CYP4A1) in liver of
rats. Dietary OFO also dose dependently increased liver microsomal CYP4A protein
(P < 0.05). The activity of hepatic ACO of the HO group was sixfold that of the HF
group (P < 0.05). Plasma total lipids, liver triglycerides, cholesterol and total lipids
were reduced in rats fed the LO and HO diets (P < 0.05). Through the ligand binding
domain of PPAR alpha, the hydrolyzed OFO enhanced the expression of alkaline
phosphatase (ALP) reporter gene to a significantly greater extent (P < 0.05) than the
hydrolyzed fresh soybean oil in a transactivation assay using a clone of CHO K1 cells
stably expressing Gal4-PPAR<alpha> chimeric receptor and UAS(4)-ALP reporter.
The results support our hypothesis that dietary OFO, by activating PPAR alpha,
up-regulates the expression of PPAR alpha downstream genes and alters lipid
metabolism in rats.

Chen, H.M., S.C. Chang, C.C. Wu, T.S. Cuo, J.S. Wu and R.H. Juang. 2001. The
catalytic behavior of L-form starch phosphorylase from sweet potato roots is
regulated by proteolysis. Physiol. Plantarum. 114(4): 506-515. (SCI)
Abstract:
Starch phosphorylase (SP) is an enzyme used for the reversible phosphorolysis of
the alpha-glucan in plant cells. When compared to its isoform in an animal cell,
glycogen phosphorylase, a peptide containing 78 amino acids (L78) is inserted in the
centre of the low-affinity type starch phosphorylase (L-SP). We found that the amino
acid sequence of L78 had several interesting features including the presence of a PEST
region, which serves as a signal for rapid degradation. Indeed, most L-SP molecules
isolated from mature sweet potato roots were nicked in the middle of a molecule, but
still retained their tertiary or quaternary structures, as well as full catalytic activity. The
nicking sites on the L78 were identified by amino acid sequencing of these peptides,
which also enabled us to propose a proteolytic process for L-SP. Enzyme kinetic
studies of L-SP in the direction of starch synthesis indicated that the K-m decreased
during the proteolytic process when starch was used as the limiting substrate, but the
K-m for the other substrate (Glc-1-P) increased. On the other hand, the maximum
velocities (V-max) increased for both substrates. Mobility of the nicked L-SP was
retarded on a native polyacrylamide gel containing soluble starch, indicating the
— 46 —
increased affinity for starch. Results in this study suggested that L78 and its
proteolytic modifications might play a regulatory role on the catalytic behaviour of
L-SP in starch biosynthesis.

Chen, C.Y., W.T. Wu, M.H. Lin, C.K. Ch’ang, H.J. Huang, J.M. Liao, L.Y. Chen
and Y.T. Liu. 2001. A common precusor for the three subunits of L-glutamate
oxidase from Streptomycess platensis NTU3304. Canadian Journal of
Microbiology. 47:269-275. (SCI)
Abstract:
A segment of DNA containing the L-glutamate oxidase (gm) gene from
Streptomyces platensis NTU3304 was cloned. The entire nucleotide sequence of the
protein-coding portion consisting of 2130 bp (710 codons, including AUG and UGA)
of the cloned DNA fragment was determined. The gm gene contained only one open
reading frame (ORF) which coded for a 78-kDa polypeptide, the precursor of active
extracellular Cox. Mature Cox is composed of three subunits, designated as alpha, beta,
and gamma, with molecular masses of 39, 19, and 16 kDa, respectively. Analyses of
the N-terminal amino acid sequences of the subunits revealed that the order of
subunits in the precursor polypeptide encoded by the ORF, from N-terminus to
C-terminus, is alpha-gamma-beta. The presence of the flavin adenine dinucleotide
(FAD)binding motif place Cox as a member of the flavoenzyme family. Furthermore,
a negative effect of glucose on the biosynthesis of Gox was observed when it was used
as carbon source.

Chou, S.F. and C.Y. Chen. 2001. Production and purification of monoclonal and
polyclonal antibodies against human ferritin, a nonspecific tumor marker.
Hybridoma 20(1):59-62. (SCI)
Abstract:
The aim of this study was to produce monoclonal and polyclonal antibodies
against a nonspecific tumor marker, human ferritin, Hyperimmune ICR mice produced
polyclonal antibodies after injection with 0.5 mL pristane, and were injected with
NS-1 myeloma cells 2 weeks later. Hyperimmune Balb/c mice were used for the
production of monoclonal antibodies (MAbs), Mice were immunized four times, given
a final boost, and their spleen cells were collected and fused with NS-1 myeloma cells
under the presence of PEG 1500, The fused cells were then selected in the
HAT-RPMIX medium. Anti-ferritin antibody-secreting hybridoma cell lines with high
titer were cloned by enzyme-linked immunoadsorbent assay (ELISA) and then
subcloned by limiting dilution in 15% fetal bovine serum (FBS) HT-RPMIX medium.
Five murine hybridoma-producing anti-ferritin MAbs were obtained and designated
1AD11F9, 1AD11E11, 2AD11D2, 2AD11A5, and 3AD11G8, Isotypes of these MAbs
— 47 —
were identified as IgM heavy chain and kappa light chain. Hitrap Protein A and Hitrap
IgM purification column were used for the purification of polyclonal and monoclonal
antibodies, respectively.

Chou, S.F., W.L. Hsu, C.M. Hwang and C.Y. Chen. 2001. Development of an
immunosensor of human ferritin, a nonspecific tumor marker. Analytica Chimica
Acta, 453 (2): 181-189. (SCI)
Abstract:
A new human ferritin immunosensor was developed using anti-human ferritin
antibodies (Abs) immobilized on the gold disc of a quartz crystal microbalance
(QCM). Two kinds of self-assembled monolayers (SAMs) prepared by
cystamine-glutaraldehyde and cystamine method were applied to immobilize
anti-ferritin monoclonal antibodies (MoAbs) and polyclonal antibodies (PoAbs) on the
quartz, respectively. The reusabilities of quartz crystal adopting the SAMs were found
to be better than those of the other immobilization methods used. The 10 cycles of
measurements could be performed on the gold surface of the same crystal regenerated
with a solution of glycine.HC1. This sensor system could be continuously performed
for 15 days, the relative frequency shifts (the frequency shifts measured are relative to
the response at the first day) were all found to be above 95%. A linear relationship
existed between the frequency shifts (Hz) and the log values of human ferritin
concentrations in the range from 0.1 to 100 ng/ml in buffer and mouse serum. This
human ferritin immumosensor had some advantages: high sensitivity, high specificity,
low sample requirement, high reusability, no label and no pretreatment etc.

Chen, P.Y., B.G. Gopalacushina, C.C. Yang, S.I. Chan and P.A. Evans. 2001. The
role of a -buldge in the folding of the –hairpin structure in ubiquitin. Protein
Sci. 10: 2075-2082. (SCI)
Abstract:
It is known that the peptide corresponding to the N-terminal beta -hairpin of
ubiquitin, U(1-17), can populate the monomeric beta -hairpin conformation in aqueous
solution. In this study, we show that the Gly-10 that forms the bulge of the beta -turn
in this hairpin is very important to the stability of the hairpin. The deletion of this
residue to desG10(1-16) unfolds the structure of the peptide in water. Even under
denaturing conditions, this bulge appears to be important in maintaining the residual
structure of ubiquitin, which involves tertiary interactions within the sequence 1 to 34
in the denatured state. We surmise that this residual structure functions as one of the
nucleation centers in the folding process and is important in stabilizing the transition
state. In accordance with this idea, deleting Gly-10 slows down the refolding and
unfolding rate by about one half.
— 48 —

Lee, K.T., T. Hirano, T. Yamakawa, T. Kodama, Y. Igarashi and K. Shimomura.
2001. Responses of transformed root culture of Atropa belladonna to salicylic
acid. J. Biosci. Bioeng. 91: 586-589. (SCI)
Abstract:
The effect of salicylic acid (SA) on tropane alkaloid production and the responses
to SA stress of transformed root cultures of Atropa belladonna (belladonna) were
investigated. Treatment of A. belladonna transformed roots with 0.2 mM SA did not
have any effect on tropane alkaloid production, but two compounds were produced in
the medium. These were identified as the SA derivatives methylsalicylate and
methyl-o-methoxybenzoate by high-resolution mass spectrometry and UV
spectrometry. In contrast, treatment with 2 mM salicylic acid stimulated tropane
alkaloid release from the transformed roots into the medium by up to 35% of the total
alkaloids after 24 h, and the SA derivatives were not observed in the medium. These
results revealed that transformed root of A. belladonna exhibits distinct by different
responses to SA stress depending on the SA concentration.
— 49 —
第七項
學生活動

九十學年度(2001 年 8 月 1 日~2002 年 7 月 31 日)

系學會幹部名單：
會
長：蔡孟男
副 會 長：郭榮彥
總務部長：吳婉如
活動部長：張愷芬
庶務部長：張恩誠
學術部長：林育新
美宣部長：張家禎
體育部長：吳宗諭
公關部長：張韶芩
系學會從九十學年度起由大二的同學擔任幹部，除了負責舉辦定期性的活動
外，也擔任同學與系上的溝通管道，傳達老師們的意見及學生們的心聲。當然，本
系的學生對運動也是相當的熱衷，志同道合的同學們及老師們自行組成球隊為系上
爭取榮耀。

籃球隊：
隊長：吳宗諭
隊員：凌啟鴻、李啟睿、蔡孟男、郭榮彥、吳泰均、黃冠綸、張耀仁、張益昌、
江中成、謝秉宏、徐柏瑋、廖健廷、林洋鼎、許銘志、郭憲季、張惟程、
林文謙、楊明唐、李培儒、季彥達、余忠佑。

排球隊：
隊長：陳泰宏
隊員：莊楷煦、鄭傑洋、蕭凇云、陳泰邦、劉楊威、陳佑誠、周大為、林煒祥、
潘士賢、鄭培益、徐柏瑋。
我們的排球隊在一位博士班學長的帶領及一群熱衷於排球的學長們苦心經
營之下，歷年來不管是在台大盃和食營盃等校際或是全國性的排球比賽中，時
常打入決賽，爭奪冠亞軍。

壘球隊：
隊長：鍾祖弘
隊員：李家興、宗培倫、王仁彥、謝清洲、賴鴻裕、簡士濠、高政毅、陳碧子、
梁雅芳、江博能、楊登旭、鄒裕民、馮志峰、蘇立賢、劉曜文、謝淑敏、
— 50 —
吳森博、吳佳其、陳世勳、張恩誠、張耀仁、陳俊帆、吳勝哲。
我們的壘球隊也是個新成立的球隊，由一些研究所的學長及老師共同和大
學部的學生主成的一支壘球隊，在第一次的比賽中(台大慢壘盃)就獲得的殿軍。

2001 年系學會活動一覽表：
活動名稱及日期
杜鵑花節
2001/4/30
活動內容
地點
這一年一度的重大節目，讓我們共襄盛舉，了解農化系，讓家長 台大舊體育
以及學生們都驚嘆不已！
館
系服設計及製作 這一次我們的系服強調全方位的農化系，經由大家的票選，圖案
2001/5/3
由染色體，蛋糕等圖案當選為 2001 農化系服。
系刊編輯及出刊 編輯系刊一直是一項很種要的活動，不僅是對外宣傳我們的系，
2001/3 月~5 月
更是與系友傳達訊息的一個重要的工作。
送舊晚會
2001/5/24
驪歌響起，又是這個悲傷的時刻。師長，同學們，我們即將各奔 天湘園
前程。風蕭蕭兮易水寒，狀士一去不復返！
農化營
為了使高中生了解本系所涵蓋的領域，引發其對於生化，微生物，台大校總區
土壤環境，食品營養的興趣，並且提供他們在選擇大學科系時能
有更開闊的眼界，本系特於暑假期間辦兩梯次的農化營，盼能以
寓教於樂的方式使高中生了解農化系的前景。
2001/7/5~13
迎新宿營
2001/9/11~12
新生入學指導
2001/9/13
食品營養盃
2001/12/13~16
農化之夜
為了迎接學弟妹們，我們特地的籌畫了這活動，讓他們能在開學 金山青年活
先認識彼此及學長姐們。
動中心
為了讓學弟妹們能在開學前對自己的系所有所了解，系上的老師 台大總圖書
精心為新生們準備了系所的介紹，引領學弟妹們進入農化的世界。館會議室
由嘉義大學食品系所舉辦的全國大專院校食品營養盃運動大會。 嘉義大學
由大學部二年級的同學為全系帶來的精采演出。
2001/12/26
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台大視聽小
劇場

九十年度各項獎項得獎名單：
台灣石化合成股份有限公司獎學金：黃依倫、張瑋成
財團法人農友社社會福利基金會獎學金：馮志峰、鄭江敏
蔡錫舜教授獎學金：鄭江敏、何貞瑩
何芳陔、劉伯文、陳振鐸教授獎學金：周明鈺
陳安泰醫師獎學金：黃嘉佳
郭錫琉先生文教基金會獎學金：王信傑、陳盟靜、常振鎧

應屆畢業生服務課程成績優良：江承先、李聖聿、蔡宏基、吳尚璈

大學部成績優良(書卷獎)：
89 學年度第 2 學期
大一：張峰銓、張書綸、楊舒婷、陳思維、張意苓
大二：林采菱、曾苔薇、江宜臻、張瑋成、周明鈺
大三：黃依倫、黃筑君、楊伊瑋、黃文寧、李幸芳
90 學年度第 1 學期
大一：張哲源、蘇育逸、范嫻蘄、賈德韻、陳永如、陳彥鴒
大二：張書綸、許弼強、簡文俐、楊舒婷、陳思維
大三：劉雨庭、高歆嵐、江宜臻、曾光靖、謝依庭、駱亭
大四：黃依倫、黃維君、楊乃縈、楊伊瑋、謝孟荔
— 52 —