TENTATIVE LAB SCHEDULE WEEK OF APRIL 15
TUESDAY
CULTIVATION AND GROWTH OF BACTERIAL CULTURES –SECTION 2
LAB EXERCISE 2-1 BACTERIAL COLONY MORPHOLOGY
YOU WILL NOT DO THE ACTIVITIES FOR DAY ONE. INSTEAD USE THE PLATES
PROVIDED TO DO THE ACTIVITIES FOR DAY TWO.
EACH STUDENT PAIR WILL NEED:
CHROMOBACTERIUM VIOLACEUM (Chr. Violaceum)
1 PLATE CULTURE
MICROCOCCUS ROSEUS (M. roseus)
1 PLATE CULTURE
PSEUDOMONAS AERUGINOSA (Ps. Aeruginosa) )
1 PLATE CULTURE
SERRATIA MARCESENS (S. marcesens)
1 PLATE CULTURE
CLOSTRIDIUM SPP. (C. spp)
1 BROTH CULTURE
MORAXELLA SPP (M. spp) or
BRAHAMELLA SPP (B.spp)
1 BROTH CULTURE
E. COLI
1 BROTH CULTURE
EACH STUDENT WILL NEED:
1 MARKING PEN
1 PLASTIC RULER
LAB EXERCISE 2-2
GROWTH PATTERNS IN BROTH
YOU WILL USE THE BACTERIAL CULTURES PROVIDED FOR LAB EXERCISE 2-1
EACH STUDENT WILL NEED
1 INOCULATING NEEDLE
1 INOCULATING LOOP
1 TE ST TUBE RACK
5 NUTRIENT BROTH TUBES
LAB EXERCISE 2-3
AEROTOLERANCE
YOU WILL USE THE BACTERIAL CULTURES PROVIDE FOR LAB EXERCISE 2-1
EACH STUDENT WILL NEED:
2
NUTRIENT AGAR DEEPS
LAB EXERCISE 2-4
YOUR INSTRUCTOR WILL INSTRUCT YOU ON WHICH ORGANISMS TO USE TO
INOCULATE.
EACH STUDENT WILL NEED:
2 THIOGLYCOLATE BROTHS
TENTATIVE LAB SCHEDULE WEEK OF 15 APRIL
THURSDAY
YOU WILL INTERPRET YOUR RESULTS FROM TUESDAY’S LAB
MICROSCOPY AND STAINING --- SECTION 3
BASIC LIGHT MICROSCOPY
EXERCISE 3-1 EXAMINATION OF PREPARED MICROSCOPE SLIDES
EXERCISE 3-2 CALIBRATION OF OCULAR MICROMETER
EACH TABLE WILL NEED:
SLANT CULTURE OF :
MICROCOCCUS LUTEUS (M. luteus)
STAPHYLOCOCCUS EPIDERMIS (S. epidermis)
BACILLUS CEREUS (B. cereus)
ESCHERICHIA COLI (E. coli)
MYCOBACTERIUM PHLEI (M. phlei)
KLEBSIELLA PNUEMONIA (K. pneumoniae)
PROTEUS VULGARIS (P. vulgaris)
MORAXELLA (M. catarrhalis) OR
BRAHEMELLA CATARRHALIS (B. catarrhalis)
gram +, acid fast –
gram +, acid fast –
gram +, acid fast –
gram -, acid fast gram +, acid fast +
gram -, acid fast –
gram – acid fast gram- acid fast -
EXERCISE 3-3 PREPARING A BACTERIAL SMEAR
EXERCISE 3-4 SIMPLE STAIN
EXERCISE 3-5 NEGATIVE STAIN
EXERCISE 3-6 GRAM STAIN
EXERCISE 3-7 ACID FAST STAIN
EXERCISE 3-8 CAPSULAR STAIN (USE HANDOUT RATHER THAN BOOK)
K. pneumoniae gives a positive result
S. epidermis gives a negative result
EXERCISE 3-9
SPORE STAIN
P. vulgaris gives a negative result
B. cereus gives a positive result
BE SURE TO MAKE SLIDES THAT SHOW BOTH NEGATIVE AND POSITIVE RESULTS.
HANGING DROP AND WET MOUNT PREPARATIONS
EXERCISE 3-11