Name_____________________
1. Obtain a leaf and paint a small portion of the leaf with clear nail
polish. While you are waiting for the nail polish to dry complete
Steps 2 and 3.
2. Calculate the area of your leaf in mm using the
equation Area = . Assume that the leaf is a circle
and use a ruler to measure the diameter of the leaf
in mm.
Area = 3.14 x (½ of the leaf diameter) (Remember the
radius is ½ of the diameter)
Area of the leaf = ___________________mm
3. The diameter of the field of view for the high power
objective (65x) is 0.7mm. Use the diameter to
calculate the area of the field of view with the
equation Area = 
Area = 3.14 x (0.35)(Remember the radius is ½ of the diameter)
Area of the field of view = ___________________mm
4. If your nail polish is dry put a piece of clear tape over the nail
polish. Press on the tape to make sure it is stuck on the nail
polish. Pull the tape off the leaf and stick it to a slide. View the
slide under the microscope and count and record the number of
stomata that you can see in one field of view using high power.
Repeat the count two more times for two different fields of view and
then calculate the average number of stomata per field of view.
Name_____________________
1. Obtain a leaf and paint a small portion of the leaf with clear nail
polish. While you are waiting for the nail polish to dry complete
Steps 2 and 3.
2. Calculate the area of your leaf in mm using the
equation Area = . Assume that the leaf is a circle
and use a ruler to measure the diameter of the leaf
in mm.
Area = 3.14 x (½ of the leaf diameter) (Remember the
radius is ½ of the diameter)
Area of the leaf = ___________________mm
3. The diameter of the field of view for the high power objective
(65x) is 0.7mm. Use the diameter to calculate the area of the field
of view with the equation Area = 
Area = 3.14 x (0.35)(Remember the radius is ½ of the diameter)
Area of the field of view = ___________________mm
4. If your nail polish is dry put a piece of clear tape over the nail
polish. Press on the tape to make sure it is stuck on the nail
polish. Pull the tape off the leaf and stick it to a slide. View the
slide under the microscope and count and record the number of
stomata that you can see in one field of view using high power.
Repeat the count two more times for two different fields of view and
then calculate the average number of stomata per field of view.
Field of view 1:___________________ stomata
Field of view 2:___________________ stomata
Field of view 3:___________________ stomata
Average =___________________ stomata
5. Take the average number of stomata (from step 4) and divide it by
the area of your field of view (from step 3). This gives you the
number of stomata per mm
Average number of stomata/Area of field of view = stomata per mm
________________________stomata per mm
6. Multiply the leaf area (from step 2)
mm (from step 5) to get an estimate
the lower surface of one leaf.
Leaf area x number of stomata per mm
leaf
________________________ = number
surface of one leaf
by the number of stomata per
of the number of stomata on
= number of stomata on one
of stomata on the lower
7. What are stomata and what is their function?
8. Where are stomata found on a leaf?
9. Draw a picture of a stoma that you observed under the microscope.
Field of view 1:___________________ stomata
Field of view 2:___________________ stomata
Field of view 3:___________________ stomata
Average =___________________ stomata
5. Take the average number of stomata (from step 4) and divide it by
the area of your field of view (from step 3). This give you the
number of stomata per mm
Average number of stomata/Area of field of view = stomata per mm
________________________stomata per mm
6. Multiply the leaf area (from step 2) by the number of stomata per
mm (from step 5) to get an estimate of the number of stomata on
the lower surface of one leaf.
Leaf area x number of stomata per mm = number of stomata on one
leaf
________________________ = number of stomata on the lower surface of
one leaf
7. What are stomata and what is their function?
8. Where are stomata found on a leaf?
9. Draw a picture of a stoma that you observed under the
microscope.