THE MINISTRY OF NATIONAL EDUCATION
GANESHA UNIVERSITY OF EDUCATION
SYLLABUS
SUBJECT MATTER
:
BIOTEKHNOLOGI
CODE
:
CU/HOURS
:
2/2
LECTURER
:
Prof. Dr NI PUTU RISTIATI, M.Pd
Dr. NI LUH PUTU MANIK WIDIYANTI, M.Kes
STUDY PROGRAM
:
PENDIDIKAN SAINS
PROGRAM
:
PASCA SARJANA
1
1. Standar Kompetensi:
Mahasiswa memahami tentang prinsip dasar dan prosedur serta penerapan aktivitas biotekhnologi dalam dunia kehidupan
2. Kompetensi Dasar:
1. Mahasiswa menguasai pemahaman teoritik tentang bentuk-bentuk tekhnologi yang dapat menambah nilai kemanfaatan sumber daya
hayati bagi kehidupan manusia
2. Mahasiswa memahami dan menguasai rekayasa genetika
3. Mahasiwa memahami kloning molekuler untuk menghasilkan keturunan yang memiliki sifat unggul
3. Deskripsi Mata Kuliah:
1. Bentuk tekhnologi yang dapat menambah nilai kemanfaatan sumber daya hayati bagi kehidupan manusia : prinsip dasar biotekhnologi,
peranan biotekhnologi dalam perbaikan sifat makhluk hidup secara fenotif dan genotif, peranan biotekhnologi dalam memproduksi
bahan-bahan alami, keamanan penggunaan OHMG dan produknya
2. Rekayasa genetika : prinsip rekayasa genetika dan aplikasinya dalam kehidupan
3. Kloning molekuler untuk menghasilkan keturunan yang memiliki sifat unggul, system cloning gen, vektor plasmid, vektor bakteriofage,
cosmid, vektor eukariotik, cDNA pustaka, prosedur penyaringan, karakterisasi klon, pengurutan asam nukleat, Polymerase Chain
Reaction, Klon gen, mutagenesis klon, aplikasi kloning dalam bidang farmasi dan dalam bidang kedokteran
4. Evaluasi
No
1
2
3
Jenis Evaluasi
Makalah dan Presentasi
Ujian Tengah Semester
Ujian Akhir Semester
Jumlah
Metode Evaluasi
Asesmen autentik
Tes Komprehensif
Tes Komprehensif
Jenis Instrumen
Presentasi and Laporan
Tes Terstruktur
Test terstruktur
Persentase (%)
20
35
45
100
2
5. Referensi
1. Abbas, A.K., Lichman, A.H., Pober, J.S. 2000. Cellular and Molecular Immunology. 4 ed. Philadelphia, Pennsylvania: W.B. Saunders
Company
2. Alberts, B., Bray, D., Hopkins, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. 2010. Cell Biology. New York. 2 Park
Square. Milton Park. Abingdon : Garland Science, Taylor & Francis Group
3. Blincko, S., Edwards, R., Howes, I., Little, J. 1996. Immunoassays. Edited by Edwards, R. Series Editors : Rickwood, D., Hames, B.D.
Chichester. New York. Brisbane. Toronto. Singapore : John Wiley & Sons
4. Brock, Madigan, Martinko. 2003. Biology of Microorganism. New York : Prentice Hall
5. Cano, C. 1986. Microbiology. St. Paul : West Publishing Company
6. Hyde, R.M. 1995. Immunology. 3rd.ed. Philadelphia. Baltimore. Hong Kong. London. Munich. Sydney. Tokyo : Williams & Wilkins
7. Nasir, M. 2002. Biotekhnologi. Potensi dan Keberhasilannya dalam Bidang Pertanian.Jakarta : PT RadjaGrafindo Persada.
8. Turner, P.C., Bates, A.D., White, M.R.H. 2000. Molecular Biology. Oxford : BIOS. Scientific Limited
9. Weaver, R.F. 2003. Molecular Biology. Singapore : McGraw-Hill
3
THE MINISTRY OF NATIONAL EDUCATION
GANESHA UNIVERSITY OF EDUCATION
LESSON UNIT
SUBJECT MATTER
:
BIOTECHNOLOGY
CODE
:
CU/HOURS
:
2/2
LECTURER
:
Prof. Dr NI PUTU RISTIATI, M.Pd
Dr. NI LUH PUTU MANIK WIDIYANTI, M.Kes
STUDY PROGRAM
:
PENDIDIKAN SAINS
PROGRAM
:
PASCA SARJANA
4
Standard of Competency
Week
Basic
Competency
1-7
Understanding
basic principles
biotechnology
: Understanding basic principles and procedure application biotechnology in human being
Learning Materials
1. Biotechnology
 Concept
 Role of gene in cell
 DNA : the main
genetics material
 Genetics code
 A part of genetics to
control gene
expression
 Method for produce
DNA molecule
recombinant
Understanding
role of
biotechnology to
produce natural
product
2. Several types
production genetics
engineering of
organisms including
industrial pharmacy,
veterinary, plant,
foodstuff, industry,
and environment
3. Potency movement
Indicator
Explain the concept
biotechnology
Explain role of gene in
cell
Explain DNA as the
main genetics material
Explain genetics code
Explain a part of
genetics to control
gene expression
Explain method for
produce DNA
molecule recombinant
Learning
Method/
Time
Learning
Experience
Strategy
Allotment
Resource
Lecture-based,
reading-based and
inquiry-based
discussion on
some fundamental
concepts of
biotechnology
including role of
gene and DNA,
genetic code and
expression,
several types of
result genetics
engineering in
organisms and
mutation
Presentation 7 x 2 x 50’
Discussion
Give examples several
types production
genetics engineering of
organisms
Explain potency
movement gene of
5
and transfer gene in
organisms resulted
genetics engineering
organisms resulted
genetics engineering
Explain potency
genotype drift
4. Potency genotipe drift
Understanding
safety products
of production
genetics
engineering of
organisms
8
9-10
Middle test
Understanding
genetics
engineering and
its application
11-12
Understanding
molecular
cloning to
1. Genetics engineering
 Concept
 Genetics engineering
of insect resistance
in plant
 Genetics engineering
of herbicide
resistance
 Genetics engineering
nitrogen fixation in
plant
 Genetics
engieneering virus
resistance in plant
1. System cloning gene
 Concept
Explain concept of
genetics engineering
Explain genetics
engineering of insect
resistance in plant
Explain genetics
engineering of
herbicide resistance
Explain genetics
engineering nitrogen
fixation in plant
Explain genetics
engineering virus
resistance in plant
Lecture-based,
reading-based and
inquiry-based
discussion on
concept of
genetics
engineering and
application in
plant
Explain the concept of
system gene cloning
Explain vectors for
Lecture-based,
reading-based and
inquiry-based
1 x 2 x 50’
Presentation 2 x 2 x 50’
Discussion
Presentation 2 x 2 x 50’
Discussion
6
producing
characteristics
superior
inheritance
13
 Vectors
 Library cDNA
 Procedure selection
discussion on
system cloning
gene

Lecture-based,
reading-based and
inquiry-based
discussion on
characteristic
clone, DNA
sequencing, PCR,
gene clone,
mutagenesis
clone
Lecture-based,
reading-based and
inquiry-based
discussion of
molecular cloning
in pharmacy and
medicine




 Cloning molecular Explain molecular
cloning in pharmacy
in pharmacy
 Cloning molecular and medicine.
in medicine
14-15
16
cloning
Explain library cDNA
Explain selection
procedure
Explain characteristic
Characteristic
clone
clone
Explain DNA
DNA sequencing
Polymerase Chain sequencing
Explain PCR
Reaction
Explain gene clone
Clone gene
Mutagenesis clon Explain mutagenesis
clone
gene
Final Exam
Presentation 1x2x50’
Discussion
Presentation 2 x 2 x 50’
Discussion
1 x 2 x 50’
7
THE EVALUATION OF LEARNING RESULTS
No
Type of Evaluation
Evaluation Method
Type of Instrument
1
Assignments
Individual Assignments
Presentation and Reports
2
3
Middle test
Final Exam
Total
Comprehension Test
Comprehension Test
Structured Test
Structured Test
Percentage
(%)
20
35
45
100
Implementation Time
The end of specific
topics
Week-8
Week-16
Referencies:
1. Abbas, A.K., Lichman, A.H., Pober, J.S. 2000. Cellular and Molecular Immunology. 4 ed. Philadelphia, Pennsylvania: W.B. Saunders
Company
2. Alberts, B., Bray, D., Hopkins, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. 2010. Cell Biology. New York. 2 Park
Square. Milton Park. Abingdon : Garland Science, Taylor & Francis Group
3. Blincko, S., Edwards, R., Howes, I., Little, J. 1996. Immunoassays. Edited by Edwards, R. Series Editors: Rickwood, D., Hames, and
B.D. Chichester. New York. Brisbane. Toronto. Singapore: John Wiley & Sons
4. Brock, Madigan, Martinko. 2003. Biology of Microorganism. New York : Prentice Hall
5. Cano, C. 1986. Microbiology. St. Paul : West Publishing Company
6. Hyde, R.M. 1995. Immunology. 3rd.ed. Philadelphia. Baltimore. Hong Kong. London. Munich. Sydney. Tokyo : Williams & Wilkins
7. Jawetz, Maelnick, Adelberg. 2004. Medical Microbiology. Third edition. New York : Appleton &Lange
8. Mims, Dockrell, Goering, Roitt, Wakelin, Zuckerman. 2004. Medical Microbiology. New York : Mosby
9. Nasir, M. 2002. Biotekhnologi. Potensi dan Keberhasilannya dalam Bidang Pertanian.Jakarta: PT RadjaGrafindo Persada.
10. Turner, P.C., Bates, A.D., White, M.R.H. 2000. Molecular Biology. Oxford: BIOS. Scientific Limited
11. Weaver, R.F. 2003. Molecular Biology. Singapore : McGraw-Hill
8