Biology 205 Cellular and Developmental Biology -- Fall 2012 M, W, F 10:00, Coker 201 Part I: CELL BIOLOGY Instructors: Dr. E. D. Salmon Dr. Erin Friedman (Part 2, Dev. Biol.) 608 Fordham Hall 241 Wilson Hall 962-2354 962-4563 Office Hours: TBA Office Hours: TBA Email:[email protected] Email: [email protected] Recitation: Wilson - W: 1:00 (Rm133), 2:00 (Rm213), 3:00 (Rm133), 4:00 (Rm213); Th: 1:00 (Rm213), 2:00 (Rm213), 3:00 (Rm213), 4:00 (Rm213) TAs: Sophia Tentori, Office: ?, Email: [email protected] Rebecca Cohen, Office: ?, Email: [email protected] Office Hours: TBA Assigned text: 1) Cell Half Essential Cell Biology, Alberts et al., 3rd edition (2009) (all reading assignments are from the assigned text unless otherwise noted); 2) Development Half (starting Oct 10) Prin. of Develop., Wolpert et al., 3rd edition (2007) Course Pak: For Cell Biology at UNC Book Store Tentative syllabus: Date Aug. Sept. Oct. 22 24 27 29 31 05 07 10 12 14 17 19 21 24 26 28 01 03 05 08 W F M W F W F M W F M W F M W F M W F M Subject Introduction to Cell Biology Microscopy Biochemistry (Review) Protein Structure Membrane Structure “ Membrane Transport; Junctions Intracellular Compartments/ Protein Sorting Endocytosis and Secretion EXAM I Mitochondria “ The Cytoskeleton - Muscle The Cytoskeleton – Microtubules The Cytoskeleton – Actin The Cell Division Cycle Mitosis Mechanics “ and Spindle Assembly Checkpoint “ and Cytokinesis EXAM II Readings Chapter 1 Chapter 1 Chapter 2 Chapter 4 Chapter 11 “ Chapter 12 Chapter 15 Chapter 14 to page 477 Chapter 14 to page 477 Chapter 17 “ “ Chapter 18 Chapter 18 “ “ Recitation Schedule: Bio 205 Fall 2011 *All recitations are in Wilson 134 unless otherwise noted. Aug. 29, 30 1) GFP (http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/index.html; http://en.wikipedia.org/wiki/Green_fluorescent_protein; http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm; http://www.microscopyu.com/articles/fluorescence/index.html); 2) How to make a “good” antibody for indirect immunofluorescence microscopy Sept. 5, 6 Red Blood Cell Experiment Sept. 12, 13 TA Review problem set questions Sept. 13 Late Afternoon: REVIEW Exam #1 (TBA) -go over OLD exam questions with Dr. Salmon 1st EXAM…Friday Sept. 14 Sept. 19, 20 How cells make ATP (review of experiments) Sept. 26, 27 “Where there is life, there is motion”—Movies Oct. 3, 4 TA Review of problem set questions Oct. 7 Late Afternoon: REVIEW Exam #2 ( #TBA) -go over old exam questions with Dr. Salmon 2nd EXAM…Monday Oct. 8 Part of First Recitation Assignment to be turned-in at your first Recitation August 29 or 30: Provide typed paper about the Green Fluorescent Protein (GFP) answering the following questions: 1. In what organism was GFP discovered, how was it discovered, and what is its natural function in the organism. 2. What is the molecular weight, and 3-D shape of GFP? What is the wavelength of light that excites its green fluorescence, and what are the wavelengths of this fluorescence? 3. Who first discovered the gene for GFP and made cDNA for protein expression. 4. Who were the three scientists that shared last years Nobel Prize in chemistry for their work on GFP; what were the significant contributions of each that won them a share of the prize? 5. Who else do you think should have also been awarded a share of the prize?