Medical Microbiology Lab Syllabus, Spring 2012 Instructors: Behnaz Parhami-Seren, Ph.D. Tamarra Cadd Elmfors, Ph.D. bpseren@gmail.com tlcaddelmfors@pipeline.sbcc.edu BMS Bio-medical Sciences Study of disease-causing organisms and human host response. Bacteriology, virology, mycology and parasitology; biochemistry, immunology and medical control. (TR: CSU/UC) *PREREQ: Chemistry 101 or 104, or 155. *SKILLS ADVISORY: Eligibility for English 110 or 110H or 110GB. Books/Course Materials 1. Microbiology W/Diseases By Taxonomy, Robert Bauman, ISBN: 9780321640437, 3rd Ed. 2. Microbiology Lab Manual, Cappuccino & Sherman Microbiology, ISBN: 9780321651334, 9th Ed. 3. Photographic Atlas For Microbiology Laboratory,Leboffe and Pierce, ISBN: 9780895828729, 4th Ed. 1 Materials: A $40 materials fee is charged that covers expendable laboratory supplies, lecture and laboratory packets, handouts and miscellaneous lab items Course Corequisites: NONE Title: BMS 127 Laboratory Medical Microbiology CRN: 55014 Instructor: Behnaz Parhami-Seren, Ph.D. Tel: 805-965-0581 Ex 2311 Office: EBS 211 Section Corequisites: NONE Bldg/Room: EBS 313 Earth and Biological Sciences Meeting Time M W 12:45 PM-02:05 PM + 0.6 HR Room EBS 301 M W 9:35 AM-12:25 PM (Lab group 1) Room EBS 313 M W 02:20 PM-05:10 PM (Lab group 2) Room EBS 313 M W 6:00 PM-8:50 PM (Lab group 3) Room EBS 313 Note to Lab Group 3: the on-line class has been cancelled. You must attend the 12:45 lecture sessions. 01/23/12 - 05/19/12 Critical Dates for this Course Term: Last day to add class: Last day to drop with an enrollment refund: Last day to drop without a "W": Last day to drop with a "W": 23-MAR-12 Spring 2012 04-FEB-12 04-FEB-12 05-FEB-12 2 3 4 5 6 Date Jan 23 Jan 25 Jan 30 Feb 1 Lecture topic Schedule Spring 2012 Introduction, Scope of Microbiology, History of Microbiology Eukaryotic Cells (Quiz #1 – Prefix and Suffix) Prokaryotic cells and Classification (Quiz #2 - Vocabulary) Molecules of Carbohydrates and Lipids reading assignments Chapter 1 Chapter 3 + handout Chapter 3/4/12 + handout Chapter 2 + handout Growth and Physiology of Microorganisms Feb 6 Feb 8 Feb 13 Feb 15 Feb 20 Feb 22 Feb 27 Protein and Enzymes Glycolysis, Krebs, ETS Nucleic Acid and Protein Synthesis Growth Reproduction and Genetics Holiday Interaction Between Host & Infectious Agents and Virulence Mechanisms Midterm #1 (covers material through February 15th) Chapter 2 + handouts Chapter 5 + handouts Chapter 3/7 + handouts Chapter 7 + handouts Chapter 14 Introduction to the Bacterial Diseases Feb 29 Mar 5 Mar 7 Mar 12 Mar 14 Diseases Caused by Gram Positive Bacteria Gram Negative Cocci Continue Gram Negatives and Begin Enterics Diseases Caused by Gram Negative Fastidious Bacilli Diseases Caused by Sporeformers, Gram Positive Rods, Spirochete Mar 19 Diseases Caused by Curved Rods, Acid Fast Bacilli and Mycoplasma Mar 21 Isolation Procedures and Nosocomial Infections March 26-April 1 Spring Break Apr 2 Midterm #2 (covers material through March 19th) Apr 4 Physical, Chemical and Chemotherapeutic Control of Microorganisms Apr 9 Sulfa Drugs and Antibiotics Apr 11 Antibiotics and Man-made Drugs Chapter 19 Chapter 19/20 Chapter 20 Chapter 20 Chapter 19/21 Chapter 19/21 handouts Chapter 9/10 Chapter 10 + handouts Chapter 10 + handouts Introduction to immunology and Resistance to Disease Apr 16 Apr 18 Blood Components and the First & Second Lines of Defense The Third Line of Defense / Autoimmune Disease Chapter 15 + handouts Chapter 16/17/18 Introduction to Viral, Rickettsial and Chlamydial Diseases Apr 23 Apr 25 Apr 30 May 2 May 7 Rickettsia and Chlamydia and Viruses Introduced Selected DNA Viral Diseases Midterm #3 (covers material through April 23rd) Discussion on Selected Viral Diseases Discussion on Selected Viral Diseases Chapter 21 Chapter 24/25 Chapter 24/25 Chapter 24/25 Introduction to Medical Mycology May 9 Introduction on Medical Mycology and Dermatophytes and Selected Fungal Diseases Chapter 22 FINAL EXAM, MAY 14, 2012, 11 a.m. to 1 p.m. in EBS 301 7 Lab Date 1 Jan 23 Laboratory Schedule Spring 2012 Introduction, Film “Coming Plague” (introduction to computer and Scientific Methods) 2 Jan 25 Ex 4, 5 & 6-Introduction to the microscope (Bring cutting board and sponges next lab) 3 Jan 30 Ex 1 & 2- Culturing 4 Feb 1 Handout-Metric System. (Read out Lab 3) 5 Feb 6 Ex 7, 8, 10, 11 Staining 6 Feb 8 Assigned Slide Box (use circle I-XII in packet) 7 Feb 13 Ex 15, 16, 42 & handout- Temperature and Physical Effects 8 Feb 15 Ex 17, 18, & 42 Relationship of Oxygen & Effects of Osmotic Pressure (Read out Lab 7) Feb 20 Holiday 9 Feb 22 Ex 14, 61-65 &67 – Throat, Urine and Feces (Read out Lab 8) 10 Feb 27 Ex 21-23, 29, & 30 – Enzyme Studies (Read out Lab 9) 11 Feb 29 Ex 23-26, & 31- More Enzyme Studies (Read out Lab 10) 12 Mar 5 Read out Enzymes; Schemes for Unknowns 13 Mar 7 Ex 32, 69 – Unknowns Handed Out: Observe, stain and do the indicated enzymes 14 Mar 12 Work on Unknowns and hand in Unknown results at the end of lab 15 Mar 14 Ex 45- Antiseptics and Disinfectants. Discuss Midterm #1 16 Mar 19 MIDTERM # 1 Labs begin at 10 AM, 2:30 PM and 6:00 PM 17 Mar 21 Ex 43- Antibiotics- Film on Antibiotics. (Read out Lab 16) March 26-April 1 Spring Break 18 Apr 2 Ex 48 & 49- Water Microbiology (Read out Lab 17) 19 Apr 4 Ex 47 & 60 – Teeth and Food (Read out Lab 18) 20 Apr 9 Ex 38 & 39 – Viruses. Film on Small Pox (Read out Lab 19) 21 Apr 11 Ex 35-37 – Molds and Yeast Start end of year project. Use cells in back of packets 22 Apr 16 Continue with Molds and Yeast 23 Apr 18 Ex 33 & 34 – Protizoa (Handout) 24 Apr 23 Continue with Protozoa & Start Tapeworms and Flukes (Handout) 25 Apr 25 Continue with Tapeworms and Flukes & Start Roundworms (Handout) 26 Apr 30 Continue Roundworms & Start Medical Entomology 27 May 2 Medical Entomology. 28 May 7 Finish up Projects Review for Midterm 2 29 May 9 MIDTERM #2 Labs begin at 10 AM, 2:30 PM and 6:00 PM 8 Assignment to hand in Card #1 Quiz #1 Card #2 Quiz #2 Quiz #3 Card #3 Quiz #4 Card #4 Quiz #5 Card #5 9 10 11 12 13 14 15 16 17 18 19 20